scholarly journals Azole Use in Agriculture, Horticulture, and Wood Preservation – Is It Indispensable?

2021 ◽  
Vol 11 ◽  
Author(s):  
Lise Nistrup Jørgensen ◽  
Thies Marten Heick
Keyword(s):  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Control Strategies ◽  
Agricultural Practices ◽  
World Market ◽  
Plant Diseases ◽  
Wood Preservation ◽  
Modern Agriculture ◽  
Market Shares ◽  
High Yields

Plant pathogens cause significant damage to plant products, compromising both quantities and quality. Even though many elements of agricultural practices are an integral part of reducing disease attacks, modern agriculture is still highly reliant on fungicides to guarantee high yields and product quality. The azoles, 14-alpha demethylase inhibitors, have been the fungicide class used most widely to control fungal plant diseases for more than four decades. More than 25 different azoles have been developed for the control of plant diseases in crops and the group has a world market value share of 20-25%. Azoles have proven to provide long-lasting control of many target plant pathogens and are categorized to have moderate risk for developing fungicide resistance. Field performances against many fungal pathogens have correspondingly been stable or only moderately reduced over time. Hence azoles are still, to date, considered the backbone in many control strategies and widely used as solo fungicides or as mixing partners with other fungicide groups, broadening the control spectrum as well as minimizing the overall risk of resistance development. This review describes the historic perspective of azoles, their market shares and importance for production of major crops like cereals, rice, oilseed rape, sugar beet, banana, citrus, and soybeans. In addition, information regarding use in amenity grass, in the wood preservation industry and as plant growth regulators are described. At the end of the review azoles are discussed in a wider context including future threats following stricter requirements for registration and potential impact on human health.

scholarly journals Natural gene drives offer potential pathogen control strategies in plants

2020 ◽  
Author(s):  
Donald M. Gardiner ◽  
Anca Rusu ◽  
Luke Barrett ◽  
Gavin C. Hunter ◽  
Kemal Kazan
Keyword(s):  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Control Strategies ◽  
Control Plant ◽  
Current Control ◽  
Plant Diseases ◽  
List Type ◽  
Gene Drive ◽  
Genetic Management ◽  
Pathogen Populations

SummaryGlobally, fungal pathogens cause enormous crop losses and current control practices are not always effective, economical or environmentally sustainable. Tools enabling genetic management of wild pathogen populations could potentially solve many problems associated with plant diseases.A natural gene drive from a heterologous species can be used in the globally important cereal pathogen, Fusarium graminearum, to remove pathogenic traits from contained populations of the fungus. The gene drive element became fixed in a freely crossing populations in only three generations.Repeat induce point mutation, a natural genome defence mechanism in fungi, may be useful to recall the gene drive following release, should a failsafe mechanism be required.We propose that gene drive technology is a potential tool to control plant pathogens.

scholarly journals Biochar and Trichoderma spp. in management of plant diseases caused by soilborne fungal pathogens: a review and perspective

2021 ◽  
Vol 10 (15) ◽  
pp. e296101522465
Author(s):  
Erika Valente de Medeiros ◽  
Lucas Figueira da Silva ◽  
Jenifer Sthephanie Araújo da Silva ◽  
Diogo Paes da Costa ◽  
Carlos Alberto Fragoso de Souza ◽  
...  
Keyword(s):  
Soil Properties ◽  
Fungal Pathogens ◽  
Plant Disease ◽  
Plant Pathogens ◽  
Plant Diseases ◽  
Management Tool ◽  
Production Plant ◽  
Trichoderma Spp ◽  
Health Concept ◽  
Plant Disease Management

A better understanding of the use of biochar with Trichoderma spp. (TRI), considered the most studied tool for biological control, would increase our ability to set priorities. However, no studies exist using the two inputs on plant disease management. Here, we hypothesized that biochar and TRI would be used for the management of soilborne plant pathogens, mainly due to changes in soil properties and its interactions. To test this hypothesis, this review assesses papers that used biochar and TRI against plant diseases and we summarize the handling mechanisms for each input. Biochar acts by mechanisms: induction to plant resistance, sorption of allelopathic and fungitoxic compounds, increase of beneficial microorganisms, changes the soil properties that promote health and nutrient availability. Trichoderma as biocontrol agents by different mechanisms: mycoparasitism, enzyme and secondary metabolic production, plant promoter agent, natural decomposition agent, and biological agent of bioremediation. Overall, our findings expand our knowledge about the reuse of wastes transformed in biochar combined with Trichoderma has potential perspective to formulate products as alternative management tool of plant disease caused by soilborne fungal pathogen and add important information that can be suitable for development of strategy for use in the global health concept.

scholarly journals Fungal Pathogens Affecting the Production and Quality of Medical Cannabis in Israel

Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 882
Author(s):  
Shachar Jerushalmi ◽  
Marcel Maymon ◽  
Aviv Dombrovsky ◽  
Stanley Freeman
Keyword(s):  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Pathogenic Fungi ◽  
Plant Diseases ◽  
Soilborne Pathogens ◽  
Medical Cannabis ◽  
Plant Parts ◽  
Commercial Cultivation

The use of and research on medical cannabis (MC) is becoming more common, yet there are still many challenges regarding plant diseases of this crop. For example, there is a lack of formal and professional knowledge regarding fungi that infect MC plants, and practical and effective methods for managing the casual agents of disease are limited. The purpose of this study was to identify foliar, stem, and soilborne pathogens affecting MC under commercial cultivation in Israel. The predominant major foliage pathogens were identified as Alternaria alternata and Botrytis cinerea, while the common stem and soilborne pathogens were identified as Fusarium oxysporum and F. solani. Other important fungi that were isolated from foliage were those producing various mycotoxins that can directly harm patients, such as Aspergillus spp. and Penicillium spp. The sampling and characterization of potential pathogenic fungi were conducted from infected MC plant parts that exhibited various disease symptoms. Koch postulates were conducted by inoculating healthy MC tissues and intact plants with fungi isolated from infected commercially cultivated symptomatic plants. In this study, we report on the major and most common plant pathogens of MC found in Israel, and determine the seasonal outbreak of each fungus.

Morphological and molecular based identification of Antifungal bacillus licheniformis

2017 ◽  
Vol 17 (1) ◽  
pp. 31-35
Author(s):  
B Oyuntogtokh ◽  
M Byambasuren
Keyword(s):  
Bacillus Licheniformis ◽  
Fungal Pathogens ◽  
Crop Production ◽  
Plant Pathogens ◽  
Control Plant ◽  
Plant Diseases ◽  
Bacillus Species ◽  
Bacterial Strains ◽  
Fusarium Spp ◽  
Bacterial Cultures

At present, plant diseases caused by soil borne plant pathogens have major constraints on crop production. Which include genera Fusarium spp, Phytophtora spp, Sclerotinia and Altenaria. Due to this reason, chemical fungicides are routinely used to control plant disease, which is also true in Mongolian case. However, use of these chemicals has caused various problems including environmental pollution with consequence of toxicity to human health also resistance of some pathogens to these fungicides are present. Fortunately, an alternative method to reduce the effect of these plant pathogens is the use of antagonist microorganisms. Therefore, some species of the genus Bacillus are recognized as one of the most effective biological control agent.Our research was focused to isolate Bacillus licheniformis, with antifungal potential, from indigenous sources. In the current study, 28 bacterial cultures were isolated from soil and fermented mare’s milk also named as koumiss. Isolated bacterial cultures were identified according to simplified key for the tentative identification of typical strain of Bacillus species. As a result 8 strains were positive and further screened for antifungal activity against Fusarium spp and Alternaria solani. Out of these 8 strains 5 strains are selected based on their high effectiveness against fungal pathogens and for further confirmation Polymerase Chain reaction run for effective bacterial strains using specific primers B.Lich-f and B.Lich-r. 

scholarly journals Inducing Fungus-Resistance into Plants through Biotechnology

2010 ◽  
Vol 2 (2) ◽  
pp. 14-21 ◽  
Author(s):  
Shabir Hussain WANI
Keyword(s):  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Hydrolytic Enzymes ◽  
Defense Responses ◽  
Stilbene Synthase ◽  
Fungal Disease ◽  
Plant Diseases ◽  
Fungal Cell ◽  
Oxidase Gene ◽  
Enhanced Resistance

Plant diseases are caused by a variety of plant pathogens including fungi, and their management requires the use of techniques like transgenic technology, molecular biology, and genetics. There have been attempts to use gene technology as an alternative method to protect plants from microbial diseases, in addition to the development of novel agrochemicals and the conventional breeding of resistant cultivars. Various genes have been introduced into plants, and the enhanced resistance against fungi has been demonstrated. These include: genes that express proteins, peptides, or antimicrobial compounds that are directly toxic to pathogens or that reduce their growth in situ; gene products that directly inhibit pathogen virulence products or enhance plant structural defense genes, that directly or indirectly activate general plant defense responses; and resistance genes involved in the hypersensitive response and in the interactions with virulence factors. The introduction of the tabtoxin acetyltransferase gene, the stilbene synthase gene, the ribosome-inactivation protein gene and the glucose oxidase gene brought enhanced resistance in different plants. Genes encoding hydrolytic enzymes such as chitinase and glucanase, which can deteriorate fungal cell-wall components, are attractive candidates for this approach and are preferentially used for the production of fungal disease-resistant plants. In addition to this, RNA-mediated gene silencing is being tried as a reverse tool for gene targeting in plant diseases caused by fungal pathogens. In this review, different mechanisms of fungal disease resistance through biotechnological approaches are discussed and the recent advances in fungal disease management through transgenic approach are reviewed.

scholarly journals Microbiota Associated with Sclerotia of Soilborne Fungal Pathogens – A Novel Source of Biocontrol Agents Producing Bioactive Volatiles

2019 ◽  
Vol 3 (2) ◽  
pp. 125-136 ◽  
Author(s):  
Pascal Mülner ◽  
Alessandro Bergna ◽  
Philipp Wagner ◽  
Dženana Sarajlić ◽  
Barbara Gstöttenmayr ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Control Strategies ◽  
Antagonistic Activity ◽  
Biocontrol Agents ◽  
High Yield ◽  
Rrna Gene ◽  
Strain Collection ◽  
Antifungal Volatiles ◽  
Differential Imaging

Soilborne plant pathogens are an increasing problem in modern agriculture, and their ability to survive long periods in soil as persistent sclerotia makes control and treatment particularly challenging. To develop new control strategies, we explored bacteria associated with sclerotia of Sclerotinia sclerotiorum and Rhizoctonia solani, two soilborne fungi causing high yield losses. We combined different methodological approaches to get insights into the indigenous microbiota of sclerotia, to compare it to bacterial communities of the surrounding environment, and to identify novel biocontrol agents and antifungal volatiles. Analysis of 16S rRNA gene fragment amplicons revealed significant compositional differences in the bacterial microbiomes of Rhizoctonia sclerotia, the unaffected tuber surface and surrounding soil. Moreover, distinctive bacterial lineages were associated with specific sample types. Flavobacteriaceae and Caulobacteraceae were primarily found in unaffected areas, while Phyllobacteriaceae and Bradyrhizobiaceae were associated with sclerotia of R. solani. In parallel, we studied a strain collection isolated from sclerotia of the pathogens for emission of bioactive volatile compounds. Isolates of Bacillus, Pseudomonas, and Buttiauxella exhibited high antagonistic activity toward both soilborne pathogens and were shown to produce novel, not yet described volatiles. Differential imaging showed that volatiles emitted by the antagonists altered the melanized sclerotia surface of S. sclerotiorum. Interestingly, combinations of bacterial antagonists increased inhibition of mycelial growth up to 60% when compared with single isolates. Our study showed that fungal survival structures are associated with a specific microbiome, which is also a reservoir for new biocontrol agents.

scholarly journals Electrospun Polymer-Fungicide Nanocomposites for Grapevine Protection

Polymers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3673
Author(s):  
Nasko Nachev ◽  
Mariya Spasova ◽  
Petya Tsekova ◽  
Nevena Manolova ◽  
Iliya Rashkov ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Water Solubility ◽  
Fungal Growth ◽  
Plant Diseases ◽  
Fibrous Materials ◽  
Phaeomoniella Chlamydospora ◽  
Chemical Fungicides

Nowadays, diseases in plants are a worldwide problem. Fungi represent the largest number of plant pathogens and are responsible for a range of serious plant diseases. Esca is a grapevine disease caused mainly by fungal pathogens Phaeomoniella chlamydospora (P. chlamydospora) and Phaeoacremonium aleophilum (P. aleophilum). The currently proposed methods to fight esca are not curative. In this study, polymer composites based on biodegradable polymer containing chemical fungicides with antifungal activity were successfully prepared by electrospinning. The obtained materials were hydrophobic with good mechanical properties. In vitro studies demonstrated that the fungicide release was higher from PLLA/K5N8Q fibrous mats (ca. 72% for 50 h) compared to the released drug amount from PLLA/5-Cl8Q materials (ca. 52% for 50 h), which is due to the better water-solubility of the salt. The antifungal activity of the fibrous materials against P. chlamydospora and P. aleophilum was studied as well. The incorporation of the fungicide in the biodegradable fibers resulted in the inhibition of fungal growth. The obtained materials are perspective candidates for the protection of vines from the penetration and growth of fungal pathogens.

scholarly journals Current status of plant pathogens of agricultural importance for Colombia. A review

2021 ◽  
Vol 15 (2) ◽  
Author(s):  
Tiago Miguel Marques Monteiro Amaro ◽  
Jonathan Cope ◽  
Bárbara Franco-Orozco
Keyword(s):  
Agricultural Production ◽  
Crop Production ◽  
Plant Pathogens ◽  
Agricultural Sector ◽  
Control Strategies ◽  
Plant Diseases ◽  
Current Status ◽  
Phytophthora Palmivora ◽  
Extensive Literature ◽  
Food And Agriculture

Plant disease still plays a major role in limiting agricultural production worldwide. Pathogens and pests reduce crop yield and can cause large reductions in crop quality. Colombia is no exception as it contends with many devastating pathogens that present a major threat to the country’s agricultural sector. This review is important because it highlights four of the more damaging pathogens that affect the economics of important crops in Colombia - Xanthomonas phaseoli pv. manihotis (Xpm), Fusarium oxysporum f. sp. cubense (Foc), Phytophthora palmivora, and Hemileia vastatrix. This paper was based on an extensive literature search for plant diseases in Colombia in databases such as PubMed and Google Scholar. Moreover, this search was complemented with research on crop production in the country in databases made available by the Food and Agriculture Organization of the United Nations (FAO). The four pathogens reviewed in this paper were chosen not only because of their current devastating effects on Colombia’s agricultural production but also because of their potential to cause further damage in the near future. Understanding the current situation of these crop pathogens in Colombia is imperative for state directives aimed at developing informed and efficient control strategies.

scholarly journals Control Of Plant Diseases By An Allium-based Antimicrobial Formulation

2022 ◽  
Author(s):  
Oladejo Oluwashina ◽  
Jafargholi Imani
Keyword(s):  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Fruits And Vegetables ◽  
Antimicrobial Properties ◽  
Field Application ◽  
Plant Diseases ◽  
In Planta ◽  
Xanthomonas Fragariae ◽  
Alternaria Dauci

The objective of this work was to determine the antimicrobial properties of an allium-based antimicrobial formulation named VEG’LYS (https://phytoauxilium.com/) on the growth of plant pathogenic microorganisms such as fungi, oomycetes, and bacteria. Two anthracnose-related species of the fungal genus Colletotrichum, C. gloeosporioides, and C. fragariae, the oomycete Phytophthora cactorum and the bacterium Xanthomonas fragariae associated with strawberry plants and two fungi Alternaria dauci and Botrytis cinerea, associated with carrot plants were tested in vitro. In in planta experiments, A. dauci and B. cinerea were used.. VEG’LYS inhibited the growth of all plant pathogens tested. We found that both curative and preventive in planta treatments with VEG’LYS inhibited the growth of A. dauci and B. cinerea in carrot. Furthermore, after spraying VEG’LYS on carrot plants the expression of the Pathogenesis-related (PR) 10 gene correlated with the magnitude of infection both in treated and untreated plants. Additionally, it has been shown, that the field application of VEG’LYS on strawberry plants results in a reduction of bacterial and fungal pathogens of strawberry fruits stored in refrigerator. In summary, VEG’LYS is a potential resistance inducer that seems to be suitable for use in both curative and preventive treatments to reduce the diseases and rotting of fruits and vegetables caused by different plant pathogens.

scholarly journals Aplicaciones de la bacteria entomopatógena Bacillus thuringiensis en el control de fitopatógenos

2011 ◽  
Vol 12 (2) ◽  
pp. 129 ◽  
Author(s):  
Bertha Carreras S.
Keyword(s):  
Bacillus Thuringiensis ◽  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Control Strategies ◽  
Wide Spectrum ◽  
Control Plant ◽  
Trichoderma Spp ◽  
Pseudomonas Spp ◽  
Biological Insecticide ◽  
Bacillus Spp

<p>El uso excesivo de plaguicidas químicos provoca resis tencia en los fitopatógenos, influencia negativa sobre el ambiente y la salud humana, por lo que se impone la implantación de estrategias de control de microorganismos benéficos, como Trichoderma spp., Bacillus spp., Pseudomonas spp. y otros agentes promisorios. Bacillus thuringiensis es el insecticida biológico más utilizado en el mundo para controlar diversos insectos y organismos plaga que afectan la agricultura, la actividad forestal y que transmiten patógenos a humanos y animales. En Cuba, los productos a base de B. thuringiensis se obtienen y se utilizan desde la década de los setenta del pasado siglo, y representan más del 40% de todos los controles biológicos. Actualmente, en el Instituto de Investigaciones de Sanidad Vegetal (INISAV) se cuenta con cepas de esta especie con efectos contra diferentes insectos y organismos plaga, lo cual permite disponer de una colección que cubre un espectro de numerosas plagas que afectan varios cultivos agrícolas; sin embargo, a pesar de la especificidad, virulencia, seguridad y potencia de estas cepas contra organismos patógenos, su potencial antifúngico es desconocido, aunque se sabe que esta bacteria produce una gran diversidad de metabolitos que resultan inhibitorios de hongos fitopatógenos. En esta revisión se citan las aplicaciones de B. thuringiensis en el control de organismos fitopatógenos y de la misma se deriva la importancia de explorar estas potencialidades en las cepas que conforman la colección de B. thuringiensis del INISAV como una alternativa más al control de fitopatógenos en Cuba.</p><p> </p><p><strong>Applications of entomopathogenic bacteria Bacillus thuringiensis to control phytopathogens.</strong></p><p>The overuse of chemical pesticides causes esistance in phytopathogens and negative influences on the environment and human health; therefore, the implementation of control strategies of beneficial microorganisms such as Trichoderma spp., Bacillus spp., Pseudomonas spp., and other promising agents is compelling. Bacillus thuringiensis is the most widely used biological insecticide in the world; it controls various insects and pests that affect agriculture and forestry and transmit pathogens to humans and animals. In Cuba, products based on B. thuringiensis were obtained and have been used since the 1970s, and they represent over 40% of all biological controls. Currently, the Plant Health Research Institute (INISAV) has various strains of this species that affect insects and pest organisms in different ways. This means they have a collection that covers a wide spectrum of the many diseases affecting various agricultural crops at their disposal. However, in spite of the recognized specificity, virulence, safety, and potency of these strains against pathogens, their antifungal potential is unknown, although it is known that this bacterium produces a variety of metabolites that inhibit fungal pathogens. In this review, we explore applications B. thuringiensis to control plant pathogenic organisms; from this, we derive the importance of exploring this potential in strains that make up the INISAV collection of B. thuringiensis as an alternative method to control plant pathogens in Cuba.</p>

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