scholarly journals Comparison of Antibacterial Activity and Wound Healing in a Superficial Abrasion Mouse Model of Staphylococcus aureus Skin Infection Using Photodynamic Therapy Based on Methylene Blue or Mupirocin or Both

2021 ◽  
Vol 8 ◽  
Author(s):  
Montserrat Pérez ◽  
Pilar Robres ◽  
Bernardino Moreno ◽  
Rosa Bolea ◽  
Maria T. Verde ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Antimicrobial Activity ◽  
Wound Healing ◽  
Photodynamic Therapy ◽  
Skin Infection ◽  
Infection Model ◽  
Impaired Wound Healing ◽  
Superficial Skin ◽  
Histopathological Studies ◽  
Enhance Wound Healing

Background: Antibiotic resistance and impaired wound healing are major concerns in S. aureus superficial skin infections, and new therapies are needed. Antimicrobial photodynamic therapy (aPDT) is a new therapeutic approach for infections, but it also improves healing in many wound models.Objective: To compare the antimicrobial activity and the effects on wound healing of aPDT based on Methylene Blue (MB-aPDT) with mupirocin treatment, either alone or in combination, in superficial skin wounds of S. aureus-infected mice. Additionally, to evaluate the clinical, microbiological, and cosmetic effects on wound healing.Materials and Methods: A superficial skin infection model of S. aureus was established in SKH-1 mice. Infected wounds were treated with MB-aPDT, MB-aPDT with a daily topical mupirocin or only with mupirocin. No treatment was carried out in control animals. Daily clinical and microbiological examinations were performed until complete clinical wound healing. Histopathological studies and statistical analysis were performed at the end of the study.Results: MB-aPDT treatment induced the best wound healing compared to mupirocin alone or to mupirocin plus MB-aPDT. Superficial contraction at 24 h and a greater reduction in size at 48 h, quicker detachment of the crust, less scaling, and absence of scars were observed. Histopathological studies correlated with clinical and gross findings. By contrast, mupirocin showed the highest logaritmic reduction of S. aureus.Conclusions: MB-aPDT and mupirocin treatments are effective in a murine superficial skin infection model of S. aureus. One session of MB-aPDT was the best option for clinical wound healing and cosmetic results. The addition of mupirocin to MB-aPDT treatment improved antimicrobial activity; however, it did not enhance wound healing. No synergistic antibacterial effects were detected.

scholarly journals Evaluation of the Effect of Platelet Rich Plasma on Wound Healing in the Tongue of Normal and Streptozotocin-induced Diabetic Albino Rats: Histological, Immunohistochemical, and Ultrastructural Study

2020 ◽  
Vol 8 (A) ◽  
pp. 666-689
Author(s):  
Mary Moheb Ramzy ◽  
Tarik Ahmed Essawy ◽  
Ali Shamaa ◽  
Saher Sayed Ali Mohamed
Keyword(s):  
Wound Healing ◽  
Single Injection ◽  
Platelet Rich Plasma ◽  
Diabetic Control ◽  
Albino Rats ◽  
Average Weight ◽  
Impaired Wound Healing ◽  
Complete Wound Healing ◽  
Diabetic Wounds ◽  
Enhance Wound Healing

Background: Delayed healing of diabetic wounds has been well-documented. Currently, the use of platelet-rich plasma (PRP) has attracted great attention in many medical fields including wound healing. Aim: Histological, immunohistochemical and ultrastructural evaluation of the effect of PRP on wound healing in the tongue of normal and Streptozotocin-induced diabetic albino rats. Methodology: A total number of 108 adult male albino rats with average weight 200gm, were used in the study. The animals were classified into two main groups: non-diabetic and diabetic groups. Each group was further divided into three subgroups: non- treated wound, PRP-treatment before wound, and PRP-treatment after wound. Tongue specimens were dissected on postoperative days 1, 3, and 7. The specimens were examined histologically by H&E, immunohistochemically by p63 and vimentin, and ultra-structurally by TEM. Results:  The most accelerated wound healing was revealed in the subgroups treated with PRP before the wound, whether non-diabetic or diabetic, which occurred very early at the 3rd day postoperative in both cases. While complete wound healing was revealed at the 7th day postoperative in both the non-diabetic and diabetic subgroups treated with PRP after the wound, which was like the non-diabetic control subgroup. Whilst, the diabetic non-treated subgroup only showed partial wound healing at the 7th day postoperative. Conclusion: A single injection of PRP could be used as a prophylactic to prevent expected impaired wound healing in diabetic oral mucosal wounds and to enhance wound healing in non-diabetic wounds. PRP could be used as a therapeutic to enhance wound healing in diabetic and non-diabetic oral mucosal wounds. Key Words: platelet rich plasma, wound healing, diabetes, rat, tongue, p63, vimentin, TEM BACKGROUND: Delayed healing of diabetic wounds has been well-documented. At present, the use of platelet-rich plasma (PRP) has attracted great attention in many medical fields including wound healing. AIM: Histological, immunohistochemical, and ultrastructural evaluation of the effect of PRP on wound healing in the tongue of normal and streptozotocin-induced diabetic albino rats. METHODOLOGY: A total number of 108 adult male albino rats with average weight 200 g were used in the study. The animals were classified into two main groups: Non-diabetic and diabetic groups. Each group was further divided into three subgroups: Non-treated wound, PRP-treatment before wound, and PRP-treatment after wound. Tongue specimens were dissected on post-operative days 1, 3, and 7. The specimens were examined histologically by H&E, immunohistochemically by p63 and vimentin, and ultrastructurally by TEM. RESULTS: The most accelerated wound healing was revealed in the subgroups treated with PRP before the wound, whether non-diabetic or diabetic, which occurred very early at the 3rd day post-operative in both cases. While complete wound healing was revealed at the 7th day post-operative in both the non-diabetic and diabetic subgroups treated with PRP after the wound, which was like the non-diabetic control subgroup. While, the diabetic non-treated subgroup only showed partial wound healing at the 7th day post-operative. CONCLUSION: A single injection of PRP could be used as a prophylactic to prevent expected impaired wound healing in diabetic oral mucosal wounds and to enhance wound healing in non-diabetic wounds. PRP could be used as a therapeutic to enhance wound healing in diabetic and non-diabetic oral mucosal wounds.

scholarly journals Combination of photodynamic therapy and phototherapy for the treatment of cutaneous open wounds in dogs - case reports

2021 ◽  
Vol 91 (5) ◽  
pp. 559-564
Author(s):  
Alexandre B. A. Sampaio ◽  
◽  
Sheila C. Rahal ◽  
Marina P. Branco ◽  
Rebeca B. Abibe ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Methylene Blue ◽  
Wound Healing ◽  
Photodynamic Therapy ◽  
Sodium Chloride ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Case Reports ◽  
Light Emitting ◽  
Red Leds

The evolution of cutaneous open wounds was evaluated in five client-owned dogs treated with photodynamic therapy (PDT), mediated by methylene blue and phototherapy with light-emitting diodes (LED) as the light source. On the first day of treatment, the wound was irrigated with sterile 0.9% sodium chloride solution, the surface was dried, and 3% solution of hydrogen peroxide soaked for 1 minute. The hydrogen peroxide was mechanically removed and, immediately after, 0.01% methylene blue was used in the wound for five minutes, after which a cluster of red LEDs (650 - 670 nm wavelength, 350 mW power, and irradiance of 200 mW/cm2) was used. Subsequently, single phototherapy sessions, every 48 hours or more, were applied until wound healing. In general, 24J was used for PDT and 12J for phototherapy. In all cases, the cutaneous open wounds treated with PDT associated with phototherapy healed without complications, and it may be considered as a treatment option, especially for extensive lesions.

Methylene blue photodynamic therapy in rats' wound healing: 21 days follow-up

2015 ◽  
Author(s):  
Vanda Sanderana Macêdo Carneiro ◽  
Maria Helena Chaves de Vasconcelos Catao ◽  
Rebeca Ferraz Menezes ◽  
Natália Costa Araújo ◽  
Marleny Elizabeth Martinez Gerbi
Keyword(s):  
Methylene Blue ◽  
Wound Healing ◽  
Photodynamic Therapy

scholarly journals Antimicrobial and wound healing properties of the methanol extract of Ficus platyphylla Del. (Moraceae) stem bark

2021 ◽  
Vol 17 (2) ◽  
pp. 121-130
Author(s):  
Azeez Raji Sheidu ◽  
Abdulkadir Umar Zezi ◽  
Abubakar Ahmed ◽  
Ben Ahmed Chindo ◽  
Garba Mohammed Magaji
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Methanol Extract ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Scientific Basis ◽  
Klebsiella Pneumonia ◽  
Corynebacterium Ulcerans ◽  
Superficial Skin ◽  
Skin Excision

Ficus platyphylla (Fp) has been used in Nigerian traditional medicine for the management of pain, ulcer, wound, epilepsy and inflammation. The efficacy of its gum from the stem bark for the management of wound has been widely acclaimed among the Hausa communities of Northern Nigeria and therefore, this study was aimed at examining the antimicrobial and wound healing potential of methanol extract of F. platyphylla stem bark (MEFpSB) in order to provide scientific basis for its antimicrobial and wound healing properties. Preliminary phytochemical screening was done and antimicrobial activity using some pathogenic micro-organisms were evaluated following its wound healing effectiveness in Wistar rats using the model of superficial skin excision wound. Data were analysed using one-way analysis of variance (ANOVA) followed by Dunnet’s t-test. The results of preliminary phytochemical screeningrevealed the presence of carbohydrate, cardiac glycosides, saponins, flavonoids, triterpenes, tannins, alkaloids with anthraquinones absent. The extract showed antimicrobial activity on Staphylococcus aureus, Corynebacterium ulcerans, Escherichia coli, Klebsiella pneumonia and Salmonella typhi and significant (p< 0.05, p< 0.001) decrease in wound measurement for 11 days. This study demonstrated that MEFpSB possesses antimicrobial activity with wound healing properties that justifies the ethno-medicinal use of the plant in wound/ulcer healings. Keywords: Wound healing; Antimicrobial; Ficus platyphylla; Formulation; Topical

Absorbable Intradermal Closure of Elective Craniotomy Wounds

2008 ◽  
Vol 62 (suppl_5) ◽  
pp. ONS490-ONS492 ◽  
Author(s):  
Sergio Paolini ◽  
Roberta Morace ◽  
Giuseppe Lanzino ◽  
Paolo Missori ◽  
Giovanni Nano ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Infection ◽  
Wound Care ◽  
Bed Rest ◽  
Cerebrospinal Fluid Leakage ◽  
Consecutive Series ◽  
Oral Antibiotics ◽  
Superficial Skin ◽  
Closure Method

Abstract Objective: More and more commonly, craniotomies are being performed with minimal hair shaving to improve cosmesis and facilitate return to a normal life. In such patients, traditional sutures or metal staples are used for scalp closure. This practice requires suture removal, often perceived as a cause of discomfort by the patient. We investigate the safety and efficacy of intradermal sutures in a large, consecutive series of patients undergoing elective craniotomy. Methods: Wound healing complications were investigated in a consecutive series of 208 patients who underwent elective craniotomy during a 2-year period. In all patients, minimal shaving, performed by shaving a small strip of hair along the planned wound, was used. Scalp closure was achieved with only absorbable intradermal running sutures. All wounds were covered with sterile adhesive strips, which were kept in place for 24 hours postoperatively. Patients were followed for a mean follow-up period of 10.6 months (range, 1–23 mo). Results: All patients except two had satisfactory wound healing. One patient (0.48%) developed cerebrospinal fluid leakage, which responded to bed rest and lumbar drainage. Another patient (0.48%) had a superficial skin infection, which was successfully treated with topical wound care and oral antibiotics. Conclusion: The closure method described is safe and effective. The absence of visible sutures in the postoperative course reinforces the cosmetic advantage of no shaving and decreases discomfort associated with the removal of sutures or staples.

scholarly journals Antimicrobial and Wound Healing Activity of Gardenia aqualla Stem Bark Methanol Extract

2021 ◽  
pp. 30-38
Author(s):  
Michael Anyekema ◽  
Lesley Sahber Kindzeka ◽  
Aristide Dzelamonyuy
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Methanol Extract ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Scientific Basis ◽  
Klebsiella Pneumonia ◽  
Phytochemical Screening ◽  
Corynebacterium Ulcerans ◽  
Superficial Skin

Aim: This study was aimed at examining the antimicrobial and wound healing activities of Gardenia aqualla stem bark methanol extract (GASBME) in order to provide scientific basis for its antimicrobial and wound healing properties. Methodology: Preliminary phytochemical screening was done and antimicrobial activity determined using some pathogenic micro-organisms were evaluated following its wound healing effectiveness in Wistar rats using the model of superficial skin excision wound. Data were analysed using one-way analysis of variance (ANOVA) followed by Dunnet’s t-test. Results: The results of preliminary phytochemical screening revealed the presence of carbohydrate, cardiac glycosides, saponins, flavonoids, triterpenes, tannins, alkaloids with anthraquinones being absent. The extract showed antimicrobial activity on Staphylococcus aureus, Corynebacterium ulcerans, Escherichia coli, Klebsiella pneumonia and Salmonella typhi and significant (p< 0.05, p< 0.001) decrease in wound measurement by the 11th day. Conclusion: This study demonstrated that GASBME possesses antimicrobial activity with wound healing properties that justifies the ethno-medicinal use of the plant in wound/ulcer healings.

Photodynamic Therapy Mediated by Methylene Blue Dye in Wound Healing

2010 ◽  
Vol 28 (5) ◽  
pp. 581-587 ◽  
Author(s):  
Felipe Fornias Sperandio ◽  
Alyne Simões ◽  
Ana Cecília Corrêa Aranha ◽  
Luciana Corrêa ◽  
Suzana C. Orsini Machado de Sousa
Keyword(s):  
Methylene Blue ◽  
Wound Healing ◽  
Photodynamic Therapy ◽  
Blue Dye ◽  
Methylene Blue Dye
Sign in / Sign up

Export Citation Format

Share Document