RF Coil Setup for 31P MRSI in Tongue Cancer in vivo at 7 T

Surgery for tongue cancer often results in a major loss in quality of life. While MRI may be used to minimise the volume of excised tissue, often the full tumour extent is missed. This tumour extent may be detected with metabolic imaging. One of the main reasons for the lack of metabolic information on tongue cancer would be the absence of an x-nuclear coil with the tongue as a focus target. Metabolic MRI through 31P MRSI is known as a powerful tool to non-invasively study elevated cell proliferation and disturbed energy metabolism in tumours. Severe magnetic field non-uniformities are inherently caused by the substantial difference in magnetic susceptibilities of tissue and air in the mouth and its environs. Despite this, the wide chemical shift dispersion of 31P could still facilitate precise detection of the cell proliferation biomarkers, phospomonoesters and diesters, as well as energy metabolites ATP, inorganic phosphate, and phosphocreatine potentially mapped over the tongue or tumour in vivo. In this study, we present the first 31P MRSI data of the human tongue in vivo from healthy volunteers and a patient with a tongue tumour at 7 T MRI using a 1H 8-channel transceiver setup placed inside a body 31P transmitter, which is able to get a uniform excitation from the tongue while providing comfortable access to the patient. In addition, a user-friendly external 31P receiver array is used to provide high sensitivity (80%) comparable to an uncomfortable inner mouth loop coil positioned on the tongue. The primary aim is the demonstration of 31P metabolite profiles in the tongue and the differences between healthy and malignant tissue. Indeed, clear elevated cell proliferation expressed as enhanced phosphomonoesters is observed in the tumour vs. the healthy part of the tongue. This can be performed within a total scan duration of 30 min, comparable to clinical scans, with a spatial resolution of 1.5 cm for the 10-min 31P MRSI scan.

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Cucurbitacin B Inhibits Cell Proliferation by Regulating X-Inactive Specific Transcript Expression in Tongue Cancer

Frontiers in Oncology ◽

10.3389/fonc.2021.651648 ◽

2021 ◽

Vol 11 ◽

Author(s):

Boqiang Tao ◽

Dongxu Wang ◽

Shuo Yang ◽

Yingkun Liu ◽

Han Wu ◽

...

Keyword(s):

Cell Proliferation ◽

Tongue Cancer ◽

P53 Protein ◽

Tumor Development ◽

Migration And Invasion ◽

Cucurbitacin B ◽

Specific Transcript ◽

Human Tongue

Cucurbitacin B (CuB), a natural product, has anti-tumor effects on various cancers. In order to investigate the expression of long non-coding RNAs (lncRNA), we carried out RNA sequencing (RNA-seq) and quantitative PCR (qPCR). The data indicated that CAL27 and SCC9 tongue squamous cell carcinoma (TSCC) cells had reduced expression of X-inactive specific transcript (XIST) after CuB treatment. Moreover, our results showed increased expression of XIST in human tongue cancer. In this study, CuB treatment inhibited proliferation, migration and invasion of SCC9 cells, and induced cellular apoptosis. Interestingly, knockdown of XIST led to inhibition of cell proliferation and induced apoptosis in vitro. In addition, reduced expression of XIST suppressed cell migration and invasion. MicroRNA 29b (miR-29b) was identified as a direct target of XIST. Previous reports indicated that miR-29b regulates p53 protein. Our results suggest that increased expression of miR-29b induces cell apoptosis through p53 protein. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) system validated the role of XIST knockout in tumor development in vivo. Together, these results suggest that CuB exerts significant anti-cancer activity by regulating expression of XIST via miR-29b.

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Tumorigenicity and Validity of Fluorescence Labelled Mesenchymal and Epithelial Human Oral Cancer Cell Lines in Nude Mice

BioMed Research International ◽

10.1155/2016/4897986 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Wei Xin Cai ◽

Li Wu Zheng ◽

Li Ma ◽

Hong Zhang Huang ◽

Ru Qing Yu ◽

...

Keyword(s):

Cancer Cells ◽

Cell Lines ◽

Cancer Cell ◽

Nude Mice ◽

Tongue Cancer ◽

Cancer Cell Lines ◽

Fluorescent Imaging ◽

Cell Phenotype ◽

Human Tongue

Tumorigenicity and metastatic activity can be visually monitored in cancer cells that were labelled with stable fluorescence. The aim was to establish and validate local and distant spread of subcutaneously previously injected fluorescence transduced human tongue cancer cell lines of epithelial and mesenchymal phenotype in nude mice. A total of 32 four-week-old male athymic Balb/c nude mice were randomly allocated into 4 groups (n=8). A single dose of 0.3 mL PBS containing 1 × 107 of four different cancer cell-lines (UM1, UM1-GFP, UM2, and UM2-RFP) was injected subcutaneously into the right side of their posterolateral back. Validity assessment of the labelled cancer cells’ tumorigenicity was assessed by physical examination, imaging, and histology four weeks after the injection. The tumor take rate of cancer cells was similar in animals injected with either parental or transduced cancer cells. Transduced cancer cells in mice were easily detectable in vivo and after cryosection using fluorescent imaging. UM1 cells showed increased tumor take rate and mean tumor volume, presenting with disorganized histopathological patterns. Fluorescence labelled epithelial and mesenchymal human tongue cancer cell lines do not change in tumorigenicity or cell phenotype after injection in vivo.

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Cordycepin induces apoptosis in human tongue cancer cells in vitro and has antitumor effects in vivo

Archives of Oral Biology ◽

10.1016/j.archoralbio.2020.104846 ◽

2020 ◽

Vol 118 ◽

pp. 104846

Author(s):

Qingwei Zheng ◽

Jing Sun ◽

Wenli Li ◽

Shuangnan Li ◽

Kai Zhang

Keyword(s):

Cancer Cells ◽

Tongue Cancer ◽

Antitumor Effects ◽

Human Tongue

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Assessment of mouse liver [1-13C]pyruvate metabolism by dynamic hyperpolarized MRS

Journal of Endocrinology ◽

10.1530/joe-19-0159 ◽

2019 ◽

Vol 242 (3) ◽

pp. 251-260

Author(s):

Kasper Faarkrog Høyer ◽

Christoffer Laustsen ◽

Steffen Ringgaard ◽

Haiyun Qi ◽

Christian Østergaard Mariager ◽

...

Keyword(s):

Real Time ◽

Mr Spectroscopy ◽

High Sensitivity ◽

High Specificity ◽

Metabolic Imaging ◽

Pyruvate Metabolism ◽

Nonalcoholic Fatty Liver ◽

Constant Rate ◽

Real Time Tracking

Hyperpolarized [1-13C]pyruvate magnetic resonance (MR) spectroscopy has the unique ability to detect real-time metabolic changes in vivo owing to its high sensitivity compared with thermal MR and high specificity compared with other metabolic imaging methods. The aim of this study was to explore the potential of hyperpolarized MR spectroscopy for quantification of liver pyruvate metabolism during a hyperinsulinemic–isoglycemic clamp in mice. Hyperpolarized [1-13C]pyruvate was used for in vivo MR spectroscopy of liver pyruvate metabolism in mice. Mice were divided into two groups: (i) non-stimulated 5-h fasted mice and (ii) hyperinsulinemic-isoglycemic clamped mice. During clamp conditions, insulin and donor blood were administered at a constant rate, whereas glucose was infused to maintain isoglycemia. When steady state was reached, insulin-stimulated mice were rapidly infused with hyperpolarized [1-13C]pyruvate for real-time tracking of the dynamic distribution of metabolic derivatives from pyruvate, such as [1-13C]lactate, [1-13C]alanine and [13C]bicarbonate. Isotopomer analysis of plasma glucose confirmed 13C-incorporation from [1-13C]pyruvate into glucose was increased in fasted mice compared with insulin-stimulated mice, demonstrating an increased gluconeogenesis in fasted mice. The AUC ratios for [1-13C]alanine/[1-13C]pyruvate (38.2%), [1-13C]lactate/[1-13C]pyruvate (41.8%) and [13C]bicarbonate/[1-13C]pyruvate (169%) all increased significantly during insulin stimulation. Hyperpolarized [1-13C]pyruvate can be used for in vivo MR spectroscopy of liver pyruvate metabolism during hyperinsulinemic-isoglycemic clamp conditions. Under these conditions, insulin decreased gluconeogenesis and increased [1-13C]alanine, [1-13C]lactate and [13C]bicarbonate after a [1-13C]pyruvate bolus. This application of in vivo spectroscopy has the potential to identify impairments in specific metabolic pathways in the liver associated with obesity, insulin resistance and nonalcoholic fatty liver disease.

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Co-registered photoacoustic and ultrasound imaging for tongue cancer detection

Journal of Innovative Optical Health Sciences ◽

10.1142/s1793545818500086 ◽

2018 ◽

Vol 11 (03) ◽

pp. 1850008 ◽

Cited By ~ 2

Author(s):

Heng Guo ◽

Weizhi Qi ◽

Ming He ◽

Jian Rong ◽

Lei Xi

Keyword(s):

Ultrasound Imaging ◽

Structural Information ◽

Tongue Cancer ◽

Early Stage ◽

Signal To Noise Ratio ◽

Imaging Techniques ◽

Common Disease ◽

High Morbidity ◽

Human Tongue

Tongue cancer is an increasingly common disease with high morbidity. Besides clinical observation, biomedical imaging techniques have been investigated for early detection of tongue cancer. In this paper, we proposed a co-registered dual-modality photoacoustic (PA) and ultrasound imaging technique to simultaneously map the functional and structural information of human tongue, which has the potential to detect and diagnose tongue cancer in early stage. The imaging probe comprises a 20-MHz side-view focused transducer for ultrasound imaging and PA detection, a light path constructed by a multimode optical fiber, and a prism for PA illumination. Phantom experiments were conducted to evaluate the performance of the system including penetration depth, spatial resolution and signal-to-noise ratio. In vivo imaging of animal tumor and human tongue was carried out to show the feasibility of the proposed technique to detect tumor lesions in human tongue. The results of phantom and in vivo experiments suggest that the proposed technique has the potential to detect the early-stage cancer lesions in human tongue.

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