scholarly journals Interaction Effect Between Copy Number Variation in Salivary Amylase Locus (AMY1) and Starch Intake on Glucose Homeostasis in the Malmö Diet and Cancer Cohort

2021 ◽  
Vol 7 ◽  
Author(s):  
Aida Koder Hamid ◽  
Johanna Andersson-Assarsson ◽  
Ulrika Ericson ◽  
Emily Sonestedt
Keyword(s):  
Type 2 Diabetes ◽  
Copy Number Variation ◽  
Glucose Homeostasis ◽  
Copy Number ◽  
Inverse Association ◽  
Salivary Amylase ◽  
Cross Sectional ◽  
Copy Numbers ◽  
Number Variation

Salivary amylase initiates the digestion of starch and it has been hypothesized that salivary amylase may play a role in the development of insulin resistance and type 2 diabetes. The aim was to examine the interaction between copy number variation in the salivary amylase gene AMY1 and starch intake. We studied 3,624 adults without diabetes or elevated blood glucose in the Malmö Diet Cancer cohort. We assessed the associations and interactions between starch intake, AMY1 copies and glucose homeostasis traits (i.e., fasting plasma glucose, insulin and HOMA-IR) and risk of type 2 diabetes over an average of 18 follow-up years. AMY1 copy number was not associated with glucose, insulin or HOMA-IR. We observed a significant interaction between starch intake and AMY1 copies on insulin and HOMA-IR after adjusting for potential confounders (p < 0.05). The inverse association between starch intake and insulin and HOMA-IR was stronger in the group with 10 or more copies (Ptrend < 0.001). In addition, we observed an inverse association between starch intake and type 2 diabetes in the group with 10 or more copies (ptrend = 0.003), but not in the other groups. This cross-sectional observational study suggests that AMY1 copy numbers might interact with starch intake on glucose homeostasis traits. Interventional studies are required to determine whether individuals with high AMY1 copy numbers may benefit from a high starch intake.

scholarly journals Salivary AMY1 Copy Number Variation Modifies Age-Related Type 2 Diabetes Risk

2020 ◽  
Vol 66 (5) ◽  
pp. 718-726
Author(s):  
Yuwei Liu ◽  
Caren E Smith ◽  
Laurence D Parnell ◽  
Yu-Chi Lee ◽  
Ping An ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Copy Number Variation ◽  
Metabolic Pathway ◽  
Copy Number ◽  
Lipid Lowering ◽  
Pathway Enrichment Analysis ◽  
Copy Numbers ◽  
Number Variation ◽  
The Relationship

Abstract Background Copy number variation (CNV) in the salivary amylase gene (AMY1) modulates salivary α-amylase levels and is associated with postprandial glycemic traits. Whether AMY1-CNV plays a role in age-mediated change in insulin resistance (IR) is uncertain. Methods We measured AMY1-CNV using duplex quantitative real-time polymerase chain reaction in two studies, the Boston Puerto Rican Health Study (BPRHS, n = 749) and the Genetics of Lipid-Lowering Drug and Diet Network study (GOLDN, n = 980), and plasma metabolomic profiles in the BPRHS. We examined the interaction between AMY1-CNV and age by assessing the relationship between age with glycemic traits and type 2 diabetes (T2D) according to high or low copy numbers of the AMY1 gene. Furthermore, we investigated associations between metabolites and interacting effects of AMY1-CNV and age on T2D risk. Results We found positive associations of IR with age among subjects with low AMY1-copy-numbers in both studies. T2D was marginally correlated with age in participants with low AMY1-copy-numbers but not with high AMY1-copy-numbers in the BPRHS. Metabolic pathway enrichment analysis identified the pentose metabolic pathway based on metabolites that were associated with both IR and the interactions between AMY1-CNV and age. Moreover, in older participants, high AMY1-copy-numbers tended to be associated with lower levels of ribonic acid, erythronic acid, and arabinonic acid, all of which were positively associated with IR. Conclusions We found evidence supporting a role of AMY1-CNV in modifying the relationship between age and IR. Individuals with low AMY1-copy-numbers tend to have increased IR with advancing age.

scholarly journals Copy number variation at leptin receptor gene locus associated with metabolic traits and the risk of type 2 diabetes mellitus

BMC Genomics ◽  
2010 ◽  
Vol 11 (1) ◽  
pp. 426 ◽  
Author(s):  
Jae-Pil Jeon ◽  
Sung-Mi Shim ◽  
Hye-Young Nam ◽  
Gil-Mi Ryu ◽  
Eun-Jung Hong ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Copy Number Variation ◽  
Copy Number ◽  
Gene Locus ◽  
Leptin Receptor ◽  
Receptor Gene ◽  
Number Variation

Identification of copy number variation of CAPN10 in Thais with type 2 diabetes by multiplex PCR and denaturing high performance liquid chromatography (DHPLC)

Gene ◽  
2012 ◽  
Vol 506 (2) ◽  
pp. 383-386 ◽  
Author(s):  
Nattachet Plengvidhya ◽  
Kanjana Chanprasert ◽  
Watip Tangjittipokin ◽  
Wanna Thongnoppakhun ◽  
Pa-thai Yenchitsomanus
Keyword(s):  
Type 2 Diabetes ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Copy Number Variation ◽  
Multiplex Pcr ◽  
Copy Number ◽  
High Performance ◽  
Number Variation

scholarly journals Divergent patterns of genic copy number variation in KCNIP1 gene reveal risk locus of type 2 diabetes in Chinese population

2018 ◽  
Vol 65 (5) ◽  
pp. 537-545 ◽  
Author(s):  
Yao Xu ◽  
Weilin Shi ◽  
Ruhui Song ◽  
Wenlin Long ◽  
Hui Guo ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Copy Number Variation ◽  
Copy Number ◽  
Chinese Population ◽  
Number Variation ◽  
Risk Locus ◽  
I Gene

scholarly journals Analyses of Copy Number Variation of GK Rat Reveal New Putative Type 2 Diabetes Susceptibility Loci

PLoS ONE ◽  
2010 ◽  
Vol 5 (11) ◽  
pp. e14077 ◽  
Author(s):  
Zhi-Qiang Ye ◽  
Shen Niu ◽  
Yang Yu ◽  
Hui Yu ◽  
Bao-Hong Liu ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Copy Number Variation ◽  
Copy Number ◽  
Susceptibility Loci ◽  
Gk Rat ◽  
Diabetes Susceptibility ◽  
Number Variation

scholarly journals Characterization of Large Copy Number Variation in Mexican Type 2 Diabetes subjects

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Iván de Jesús Ascencio-Montiel ◽  
Dalila Pinto ◽  
Esteban J. Parra ◽  
Adán Valladares-Salgado ◽  
Miguel Cruz ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Copy Number Variation ◽  
Copy Number ◽  
Number Variation

scholarly journals Optimization of Droplet Digital Polymerase Chain Reaction (Ddpcr) For Dna Copy Number Variation Analysis of Cnv Esv27061 in Young Adults with Higher Blood Pressure

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Siti Radziah Shaik Alaudeen ◽  
Aszrin Abdullah ◽  
Azarisman Shah Mohd Shah ◽  
Norlelawati Abdul Talib
Keyword(s):  
Blood Pressure ◽  
Young Adults ◽  
Copy Number Variation ◽  
High Blood Pressure ◽  
Dna Sequences ◽  
Copy Number ◽  
Healthy Controls ◽  
Cross Sectional ◽  
Dna Concentration ◽  
Number Variation

Introduction: Copy number variation (CNV) caused by changes in DNA sequences of 1000 or more bases is implicated with susceptibility to common diseases. A study on CNV esv27061 among hypertensive Australian adults reported association with high blood pressure (BP). In Malaysia, no study on CNV among hypertensive young adults is available. Thus, this investigation aimed to assess the CNV esv27061 of young Malaysian adults with high blood pressure using optimized ddPCR. Materials and method: Ten samples each from hypertensive and healthy controls were randomly selected from samples collected for an on-going comparative cross-sectional research project among young adults living in Kuantan. The DNAs were purified using Maxwell RSC Buffy Coat DNA Kit and the concentration was measured using SimpliNano spectrophotometer. Subsequently, restriction digestion of DNAs by EcoRV was performed prior to ddPCR. The products were later subjected to droplet generation (QX100 Droplet Generator), PCR amplification and finally CNV was read by QX100 Droplet reader. Unfortunately, the above method did not yield any result. Thus, an alternative method in which purified DNA concentration was determined by QuantiFluor ONE dsDNA System (Quantus fluorometer). The DNAs (60 ng) and Alu1 were added in master mix during ddPCR and CNV esv27061 analysis was performed as stated above. Results: Optimization of method in this study showed that the detection of CNV esv27061 was possible by the use of more sensitive measurement of DNA concentration, Alu1 restriction enzyme instead of EcoRV and digestion in ddPCR reaction method rather than prior digestion. The finalized protocol run on selected hypertensive and healthy controls has shown to be reproducible and easily interpretable discrimination of gene's copy numbers. Conclusion: This optimized protocol for CNV esv27061 analysis proved useful in identifying CNV and will allow a reproducible assay evaluation and the application of this method to a bigger sample size.

scholarly journals Circulating calcium levels and the risk of type 2 diabetes: a systematic review and meta-analysis

2019 ◽  
Vol 122 (04) ◽  
pp. 376-387 ◽  
Author(s):  
J. Zhu ◽  
P. Xun ◽  
J. C. Bae ◽  
J. H. Kim ◽  
D. J. Kim ◽  
...  
Keyword(s):  
Systematic Review ◽  
Type 2 Diabetes ◽  
Cohort Studies ◽  
Glucose Homeostasis ◽  
De Novo ◽  
Meta Analysis ◽  
Cross Sectional ◽  
Relative Risks ◽  
Ca Homeostasis

AbstractAbnormal Ca homeostasis has been associated with impaired glucose metabolism. However, the epidemiological evidence is controversial. We aimed to assess the association between circulating Ca levels and the risk of type 2 diabetes mellitus (T2DM) or abnormal glucose homeostasis through conducting a systematic review and meta-analysis. Eligible studies were identified by searching electronic database (PubMed, Embase and Google Scholar) and related references withde novoresults from primary studies up to December 2018. A random-effects meta-analysis was performed to estimate the weighted relative risks (RR) and 95 % CI for the associations. The search yielded twenty eligible publications with eight cohort studies identified for the meta-analysis, which included a total of 89 165 participants. Comparing the highest with the lowest category of albumin-adjusted serum Ca, the pooled RR was 1·14 (95 % CI 1·05, 1·24) for T2DM (n51 489). Similarly, serum total Ca was associated with incident T2DM (RR 1·25; 95 % CI 1·10, 1·42) (n64 502). Additionally, the adjusted RR for 1 mg/dl increments in albumin-adjusted serum Ca or serum total Ca levels was 1·16 (95 % CI 1·07, 1·27) and 1·19 (95 % CI 1·11, 1·28), respectively. The observed associations remained with the inclusion of a cohort study with ionised Ca as the exposure. However, data pooled from neither case–control (n4) nor cross-sectional (n8) studies manifested a significant correlation between circulating Ca and glucose homeostasis. In conclusion, accumulated data from the cohort studies suggest that higher circulating Ca levels are associated with an augmented risk of T2DM.

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