scholarly journals Systematic Safety Evaluation of Cold Plasma-Activated Liquid in Rabbits

2021 ◽  
Vol 9 ◽  
Author(s):  
Yujing Xu ◽  
Sansan Peng ◽  
Bing Li ◽  
Shuai Wang ◽  
Hao Zhang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
New Zealand ◽  
Safety Evaluation ◽  
Toxicity Tests ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Acute Toxicity Tests ◽  
Blood Biochemical Indicators ◽  
New Zealand Rabbits

Plasma-activated liquid (PAL) can effectively and selectively kill various types of cancer cells both in superficial and deeper tumors. As a promising novel approach to oncotherapy, the safety of PAL is essential in the clinic but has not been thoroughly assessed. In myeloma and blood tumors, the pathogenesis is in the bone marrow cavity. We have therefore evaluated the safety of PAL in New Zealand rabbits by intra-bone marrow injection, and provide a basis of further clinical research and application of PALs. In this study, both a plasma jet and plasma surface were used to treat saline solution, phosphate-buffered solution, and cell culture medium, to produce PAL. Then, oscillograms and optical emission spectra were evaluated to characterize the plasma discharge. Acute toxicity tests and safety evaluation studies were conducted by intra-bone marrow injection of PAL into New Zealand rabbits, while control rabbits received saline only. Body weight, vital organ coefficient, organ appearance, organ histopathology, blood cell and hemoglobin parameters, and blood biochemical indicators were tested on the 30th day after injection. We found that there was no mortality or loss of mobility throughout the experimental period. Acute toxicity tests showed that there were no PAL-related side effects in rabbits receiving the maximum dose of 700 μL PAL. PAL treatments did not affect body weight, organ coefficient, organ appearance, organ histopathology, or blood biochemical indicators. However, the percentage of lymphocytes decreased while the percentage of neutrophil granulocytes increased compared with the control group. In summary, our results indicate that PAL can be safely injected into bone marrow of New Zealand rabbits without significant toxicity.

Effect of pig housing system on blood biochemical indicators’ level

2018 ◽  
Vol 35 (4) ◽  
pp. 45-51
Author(s):  
Maria Tietze ◽  
Wanda Krupa ◽  
Monika Budzyńska ◽  
Jarosław Kamieniak ◽  
Leszek Sołtys ◽  
...  
Keyword(s):  
Housing System ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Blood Biochemical Indicators

scholarly journals Effect of Various Feed Phosphates on Biochemical Indices of Blood and Mineral Composition of Bones in Finishing Pigs

2010 ◽  
Vol 79 (3) ◽  
pp. 355-361 ◽  
Author(s):  
Zbigniew Dobrzański ◽  
Krystyna Pogoda-Sewerniak ◽  
Szymon Dragan ◽  
Daniel Korniewicz ◽  
Krystyna Hoffmann ◽  
...  
Keyword(s):  
Lactic Dehydrogenase ◽  
Low Density Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Finishing Pigs ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Mineral Components ◽  
Blood Biochemical Indicators ◽  
Biochemical Indices

The aim of this study was to evaluate the effect of three different chemical feed phosphates on the blood biochemical indicators and the content of main minerals of bones in finishing pigs. Over a period of 85 days of fattening, monocalcium (MCP, Finnish product), dicalcium (DCP, Polish product) and calcium-sodium (CSP, Russian product) phosphates were used in fattener feeding. The feeding was based on standard mixtures of starter, grower and finisher type. Dicalcium phosphate was produced according to the new, pro-ecological technology based on phosphoric acid. The content of Ca, Na, P, solubility of P in citric acid, and the concentration of undesirable substances (As, Cd, F, Hg and Pb) were determined in feed phosphates. At the end of the fattening period, blood was collected from 36 finishing pigs (12 from each group) and the following biochemical indicators were determined in the serum: enzymatic activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), γ-glutamyltransferase (GGT), creatine kinase (CK), lactic dehydrogenase (LDH), lactic acid (LA); the concentration of total protein, albumins, glucose, urea, creatinine, content of triglycerides, cholesterol and its high density lipoproteins (HDL) and low density lipoproteins (LDL) fractions, and mineral components concentration (Ca, Cl, Cu, Fe, K, Mg, Na, P, Zn). Basic macroelement content (Ca, Mg, P) was determined in the thigh bones from 30 pigs (10 from each group). Significant differences (p < 0.05) between groups were observed only in some biochemical indicators, i.e. CK, LDH and LA. The highest content of Ca, Mg and P was found in the bones of pigs fed mixtures supplemented with DCP which indicates improved bioavailability of main macroelements from that phosphate.

Genetic parameters for the prediction of abdominal fat traits using blood biochemical indicators in broilers

2017 ◽  
Vol 59 (1) ◽  
pp. 28-33 ◽  
Author(s):  
H. L. Zhang ◽  
Z. Q. Xu ◽  
L. L. Yang ◽  
Y. X. Wang ◽  
Y. M. Li ◽  
...  
Keyword(s):  
Genetic Parameters ◽  
Abdominal Fat ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Blood Biochemical Indicators

scholarly journals Comparison of the Metabolites of Water Polo Players before and after Competition by the Metabolomic Approach

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Jingjing Wang ◽  
Mohammed Abdella Kemal
Keyword(s):  
Ketone Bodies ◽  
Urine Ph ◽  
Blood Samples ◽  
Biochemical Indicators ◽  
Water Polo ◽  
Metabolic Characteristics ◽  
Blood Biochemical ◽  
Before And After ◽  
Blood Biochemical Indicators ◽  
Metabolomic Approach

Background. The metabolic characteristics of body fluids of excellent water polo players before and after competition have not been reported. The purpose of the study was to compare the metabolites in the urine of water polo players before and after competition by 1H-NMR-based metabolomic approach. Methods. Twenty-six young water polo players participated in the study voluntarily. The urine and blood samples of players were collected one week before competition (A), immediately after competition (B), and one week after competition (C). Metabolomic analysis was conducted on the urine samples. Urine routine items and biochemical indicators in blood samples were detected. Results. Metabolomic results showed that the contents of eleven metabolites including lactic acid, acetoacetate, and succinic acid in the urine of the subjects increased and four metabolites such as dimethylamine, choline, and glucose decreased at stage B. Most metabolites at stage C had basically returned to the levels at stage A. Pyruvate metabolism, pantothenate and CoA biosynthesis, synthesis, and degradation of ketone bodies were mainly involved in the above process. Urine conventional analysis results showed that the urine pH decreased dramatically and the levels of PRO and URO significantly increased at stage B, and the three indicators had similar values between stages A and C. The other indicators did not have obvious difference among the three stages. Analysis of blood biochemical indicators showed that the levels of LDH, BUN, CK, and AST significantly increased at stage B and did not show an obvious difference between stages A and C. The results are helpful for coaches to arrange the athletes’ diet reasonably and to conduct scientific training for athletes.

scholarly journals The relationship between urinary Alzheimer-associated neuronal thread protein and blood biochemical indicators in the general population

Aging ◽  
2020 ◽  
Vol 12 (15) ◽  
pp. 15260-15280
Author(s):  
Yuxia Li ◽  
Shaochen Guan ◽  
He Jin ◽  
Hongjun Liu ◽  
Meimei Kang ◽  
...  
Keyword(s):  
General Population ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Blood Biochemical Indicators ◽  
The Relationship

scholarly journals HEPATO-NEPHROPROTECTIVE ACTIVITY OF NIGELLA SATIVA OIL ON PARACETAMOL-INDUCED NEW ZEALAND RABBITS (ORYCTOLAGUS CUNICULUS)

2017 ◽  
Vol 9 (10) ◽  
pp. 225
Author(s):  
Nadhira M. Dinar ◽  
Sheila Pratiwi ◽  
Rain Kihara ◽  
Nadia G. Paramita ◽  
Nazilla R. Fathurrahman ◽  
...  
Keyword(s):  
Body Weight ◽  
New Zealand ◽  
Oryctolagus Cuniculus ◽  
Nigella Sativa ◽  
Treated Group ◽  
Nigella Sativa Oil ◽  
Oral Group ◽  
Nephroprotective Activity ◽  
Per Oral ◽  
New Zealand Rabbits

Objective: The objective of this work was to investigate the hepato-nephroprotective activity of Nigella sativa (Ranunculaceae) oil on paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus).Methods: Hepato-nephroprotective activity of Nigella sativa oil was demonstrated on six groups of paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus) aged 3-4 mo, three in each group (2 males, 1 female). Group I was normal control (water 1.0 ml/kg of body weight per oral), group II was negative control (water 1.0 ml/kg of body weight per oral), group III was positive control (silymarin 100 mg/kg of body weight per oral), group IV-VI were treated with Nigella sativa oil (NSO) dose of 0.5 mg/kg of body weight, 1.0 mg/kg of body weight, and 2.0 mg/kg of body weight per oral, respectively, for 15 d. At the 16th day, rabbits in group II-VI were induced with paracetamol at a dose of 600 mg/kg of body weight per oral. At the 23rd day the animals were measured for their clinical biochemistry parameters and histological examination.Results: Paracetamol administration dose of 600 mg/kg of BW resulted in the elevation of serum glutamic-oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), and ureum-N levels of the animals, particularly in group II which was treated only with paracetamol. Normal histology of the liver defines the clear shape of the terminal hepatic venule (THV)/central vein (CV) and sinusoids, whereas that of the kidney defines clear shape of the Bowman capsule and glomerulus shape. Qualitative histological examination of the liver showed that the THV/CV in all groups was normal, however in the paracetamol-treated group, the sinusoids were dilated, necrosis and mass apoptosis were detected. Dilated sinusoids were observed in the silymarin group and in the lower and medium doses of NSO groups. In the highest dose of NSO group the THV/CV and sinusoids were normal, but a local apoptosis and fat degeneration were detected. Qualitative histological examination of the kidney indicated that there was no abnormality of the glomerulus shape, however, mass apoptosis and local necrosis of the kidney were found in the paracetamol-treated group and the silymarin-treated group. The lowest dose of the NSO-treated group showed a normal shape of glomerulus and Bowman capsule, normal apoptosis. No necrosis was observed in the rabbit’s kidney. Higher doses of NSO groups indicated a normal glomerulus shape and Bowman capsule, mass apoptosis and local necrosis.Conclusion: In this study, Nigella sativa oil could maintain the normality of the THV/CV and sinusoids in the liver of paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus). Normal glomerulus shape and Bowman capsule were also confirmed in the kidney of paracetamol-induced animals.

Effects of Water Temperature on Biochemical Characteristics of Mandarin Fish during Live Transportation

2014 ◽  
Vol 941-944 ◽  
pp. 1092-1098
Author(s):  
Shou Kun Cheng ◽  
Shun Sheng Chen ◽  
Li Juan Wang ◽  
Shuo Lin Huang
Keyword(s):  
Respiratory Rate ◽  
Water Temperature ◽  
Short Distance ◽  
Ammonia Nitrogen ◽  
Mandarin Fish ◽  
Long Distance ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Opposite Trend ◽  
Blood Biochemical Indicators

Objective and Methods: The effect of water temperature on Mandarin fish during live transportation was studied through testing the respiratory rate, ammonia nitrogen in water, glycogen and lactic acid in flesh and blood biochemical indicators at 20°C, 15°C,10°C and 5°C after transported for 0h, 3h, 10h and 24h. Results: The respiratory rate declined with the decreasing temperature and extending transportation time; the ammonia nitrogen in water increased with the temperature falling and lapse of time; the glycogen dropped as time went by and 24h later it declined with the temperature falling; but lactic presented a completely opposite trend; The cortisol decreased with the temperature declined, and rose first and then fell with the time; AST had a negative correlation with water temperature but GLU had a positive correlation; UREA decreased significantly with the declined temperature between 20°C and 10°C, and the level of it at 5°C was close to at 20°C.Conclutions: All these results revealed that 5°C should be selected for short distance transportation less than 10h and 10-15°C was the best choice for long distance transportation.

Age-related Changes of Selected Blood Biochemical Indicators in Dairy Calves during Their First Week of Life

2011 ◽  
Vol 59 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Agnieszka Herosimczyk ◽  
Adam Lepczyński ◽  
Alicja Dratwa-Chałupnik ◽  
Anna Kurpińska ◽  
Agnieszka Klonowska ◽  
...  
Keyword(s):  
Dairy Calves ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Age Related ◽  
Blood Biochemical Indicators ◽  
Age Related Changes

Synthesis and Safety Evaluation of a New Enteric Coating Material

2011 ◽  
Vol 266 ◽  
pp. 157-160
Author(s):  
Xiao Lin Lai ◽  
Hua Jiang Deng ◽  
Zong Hua Wang ◽  
Jian Hua Guo
Keyword(s):  
Acute Toxicity ◽  
Model Compound ◽  
Safety Evaluation ◽  
Coating Material ◽  
Toxicity Tests ◽  
Enteric Coating ◽  
Coating Materials ◽  
Catalyst Amount ◽  
Acute Toxicity Tests ◽  
Molar Percent

A series of styrene-maleic anhydride (SMA)-ethanol polymers with different degree of esterification (DE) were synthesized, they had different pH-sensitive values and could be used as enteric coating materials. SMA-ethanol (DE50%) was used as the model compound to evaluate the safety as an enteric coating material, the results of acute toxicity tests showed that there was no acute toxicity. The reaction condition such as catalysts, catalyst amount, reaction time and temperature were also evaluated, the results indicated that the triethylamine (TEA) was the optimal catalyst, when methyl ethyl ketone (MEK) was the solvent, the catalyst amount was 15-20%(molar percent), the reaction at 80 OC for 8h could gain the highest conversation.

Sign in / Sign up

Export Citation Format

Share Document