Transcriptomic and Physiological Evidence for the Relationship between Unsaturated Fatty Acid and Salt Stress in Peanut

The synthesis of triacylglycerols was investigated in microsomes (microsomal fractions) prepared from the developing cotyledons of sunflower (Helianthus annuus). Particular emphasis was placed on the mechanisms involved in controlling the C18- unsaturated-fatty-acid content of the oils. We have demonstrated that the microsomes were capable of: the transfer of oleate from acyl-CoA to position 2 of sn-phosphatidylcholine for its subsequent desaturation and the return of the polyunsaturated products to the acyl-CoA pool by further acyl exchange; the acylation of sn-glycerol 3-phosphate with acyl-CoA to yield phosphatidic acid, which was further utilized in diacyl- and tri-acylglycerol synthesis; and (3) the equilibrium of a diacylglycerol pool with phosphatidylcholine. The acyl exchange between acyl-CoA and position 2 of sn-phosphatidylcholine coupled to the equilibration of diacylglycerol and phosphatidylcholine brings about the continuous enrichment of the glycerol backbone with C18 polyunsaturated fatty acids for triacylglycerol production. Similar reactions were found to operate in another oilseed plant, safflower (Carthamus tinctorius L.). On the other hand, the microsomes of avocado (Persea americana) mesocarp, which synthesize triacylglycerol via the Kennedy [(1961) Fed. Proc. Fed. Am. Soc. Exp. Biol. 20, 934-940] pathway, were deficient in acyl exchange and the diacylglycerol in equilibrium phosphatidylcholine interconversion. The results provide a working model that helps to explain the relationship between C18- unsaturated-fatty-acid synthesis and triacylglycerol production in oilseeds.

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The relationship between altered membrane composition, eicosanoids and TNF-induced IL1 and IL6 production in macrophages of rats fed fats of different unsaturated fatty acid composition

Molecular and Cellular Biochemistry ◽

10.1007/bf00229475 ◽

1996 ◽

Vol 165 (2) ◽

Cited By ~ 7

Author(s):

ParamjitS. Tappia ◽

RobertF. Grimble

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Unsaturated Fatty Acid ◽

Membrane Composition ◽

The Relationship

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Water Deficit Modulates the Relationship between Temperature and Unsaturated Fatty Acid Profile in Soybean Seed Oil

Crop Science ◽

10.2135/cropsci2017.04.0214 ◽

2017 ◽

Vol 57 (6) ◽

pp. 3179-3189 ◽

Cited By ~ 3

Author(s):

Constanza S. Carrera ◽

Julio L. Dardanelli

Keyword(s):

Fatty Acid ◽

Water Deficit ◽

Fatty Acid Profile ◽

Unsaturated Fatty Acid ◽

Seed Oil ◽

Soybean Seed ◽

The Relationship

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Relations between Cold Resistance and Composition and Content of Fatty Acids in Leaves of Toona sinensis from Different Provenances

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.726-731.4473 ◽

2013 ◽

Vol 726-731 ◽

pp. 4473-4479 ◽

Cited By ~ 1

Author(s):

Guo Xia Wang ◽

Yu Zhen Yang

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Unsaturated Fatty Acid ◽

Acid Content ◽

Cold Resistance ◽

Fatty Acid Content ◽

Dodecanoic Acid ◽

Toona Sinensis ◽

The Relationship

The annual seedlings of ten Toona Sinensis provenances were selected for the study of the relationship between cold resistance and fatty acid composition and content of leaves. The results indicated that the fatty acid composition was the same among different provenances. The main ones were Dodecanoic acid, Hexadecanoic acid, (Z,Z)-9,12-Octadecadienoic acid, (Z,Z,Z)-9,12,15-Octadecatrienoic acid, Octadecanoic acid and Eicosanoic acid. But there were evident differences in fatty acid content and index of unsaturated fatty acid (IUFA) among different provenances. The content of unsaturated fatty acid and the IUFA was smaller in southern provenances of Xiapu of Fujian, Qianxinan of Guizhou and Dongkou of Hunan than those of the other provenances from north .The fatty acid content and IUFA were higher in provenances whose cold resistance were stronger. And the results showed that the fatty acid content and IUFA had a positive correlation with cold resistance of Toona sinensis.

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IDENTIFICATION AND CHARACTERIZATION OF A PURPLE NONSULFUR BACTERIUM ISOLATED FROM COASTAL AREA OF HAI PHONG FOR USING IN PRODUCTION OF UNSATURATED FATTY ACID (OMEGA 6, 7, 9)

Vietnam Journal of Science and Technology ◽

10.15625/2525-2518/57/6/13711 ◽

2019 ◽

Vol 57 (6) ◽

pp. 665 ◽

Cited By ~ 1

Author(s):

Yen Thi Hoang ◽

Quynh Thi Thu Tran ◽

Ha Hoang Chu ◽

Tuyen Thi Do ◽

Thanh Tat Dang ◽

...

Keyword(s):

Fatty Acid ◽

Unsaturated Fatty Acid ◽

Unsaturated Fatty Acids ◽

Nitrogen Sources ◽

16S Rrna Analysis ◽

Rhodovulum Sulfidophilum ◽

Purple Nonsulfur Bacterium ◽

High Lipid ◽

Physiological Properties ◽

Omega 6

Purple nonsulfur bacteria are a group that has so much biotechnological applications, particularly in producing of functional food rich with unsaturated fatty acids. A purple nonsulfur bacterium (named HPB.6) was chosen based on its strong growth, high lipid and synthesis of unsaturated fatty acid (omega 6,7,9). Studying on basic biological characteristics showed that the cells of HPB.6 were observed as ovoid-rod shape, none motility, Gram negative staining. The diameter of single bacterium was about 0.8-1.0 µm. The cells divide by binary fission and had bacteriochlorophyll a (Bchl a). This bacterium grew well on medium with carbon and nitrogen sources such as acetate, succinate, pyruvate, butyrate, glutamate, arginine, leucine, tyrosine, alanine, methionine, threonine, glutamine, yeast extract and NH4Cl. This selected strain grew well on medium with salt concentrations from 1.5 - 6.0% (optimum 3%), pH from 5.0 to 8.0 (optimum at pH 6.5) and could withstand Na2S at 4.0 - 5.2 mM. Based on morphological, physiological properties and 16S rRNA analysis received demonstrated that HPB.6 strain belongs to the species Rhodovulum sulfidophilum.

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TRIGLYCERIDE COMPOSITION OF SIXTEEN STRAINS OF MARINE DIATOM

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.29244/jitkt.v5i1.7762 ◽

2013 ◽

Vol 5 (1) ◽

Author(s):

Lily M.G. Panggabean ◽

Abdullah Rasyid ◽

Zarrah Duniani ◽

Yana Meliana ◽

Indah Kurniasih

Keyword(s):

Mass Spectrometry ◽

Fatty Acid ◽

Double Bond ◽

Unsaturated Fatty Acid ◽

Ion Trap ◽

Carbon Chain ◽

Highly Unsaturated Fatty Acid ◽

Marine Diatom ◽

Ion Trap Mass Spectrometry ◽

Spectra Analysis

Trigliceride or triacylglicerol (TAG) composition in crude oil of sixteen strain of marine diatom has been detected by spectra analyses on an Electrospray - Ion Trap – Mass Spectrometry (ESI-IT-MS) HCT Bruker-Daltonic GmbH instrument with AgNO3 used as coordination ionization agent. Biomass samples of each microalga strain were taken from early and late stationary cultures in f/2 enriched seawater and algal oils were extracted according to Bligh and Dyer. Results from spectra analysis showed that P-Pt-P (C16:0-C16:1-C16:0) were distinguished in TAG from diatom strains Chaetoceros sp.1, Chaetoceros sp.2, Thalasiossira sp.1, Thalasiossira sp.2, Thalasiossira sp.3, Navicula sp. 1, Navicula sp. 2, Navicula sp. 3, Navicula sp. 4, Nitzschia sp. 2 and Amphora sp. In contrast, TAGs in Melosira sp. included P-P-P (C16:0-C16:0-C16:0) and P-P-O (C16:0-C16:0-C18:1) were identified. TAGs from Chaetoceros sp. were the most varies among samples, i.e. P-Pt-P (C16:0-C16:1-C16:0), A-P-M (C20:4-C16:0-C14:0), P-Pt-Lt (C16:0-C16:1-C18:3), P-Pt-A (C16:0-C16:1-C20:4), D-P-P (C22:6-C16:0-C16:0), A-Ln-P (C20:4-C18:2-C16:0). Various TAGs were also detected in Nitzschia sp.2, i.e. P-Pt-M (C16:0-C16:1-C14:0), P-Pt-P (C16:0-C16:1-C16:0), P-Pt-S (C16:0-C16:1-C18:0), P-Pt-A (C16:0-C16:1-C20:4). TAGs composition in Skeletonema strains that similar to those in Nitzschia sp.1 has longer carbon, i.e. P-P-O (C16:0-C16:0-C18:1), P-O-O (C16:0-C18:1-C18:1) and O-O-O (C18:1-C18:1-C18:1). TAGs with longer carbon chain and more double bond including highly unsaturated fatty acid C20:4 were increased with culture age in diatoms Chaetoceros sp.1, Chaetoceros sp.2, Thalasiossira sp.2, Navicula sp.1 and Nitzschia sp. 2.Keywords: diatom, TAG, ESI-IT-MS, f/2, early and late stationary

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