scholarly journals EgmiR5179 Regulates Lipid Metabolism by Targeting EgMADS16 in the Mesocarp of Oil Palm (Elaeis guineensis)

2021 ◽  
Vol 12 ◽  
Author(s):  
Yifei Wang ◽  
Jixin Zou ◽  
Jin Zhao ◽  
Yusheng Zheng ◽  
Dongdong Li
Keyword(s):  
Lipid Metabolism ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Research Work ◽  
Acid Synthesis ◽  
Inhibitory Effect ◽  
Expression Level ◽  
Luciferase Reporter ◽  
Palm Fruit ◽  
Dual Luciferase Reporter Assay

EgMADS16, one of the MADS-box transcription factors in oil palm, has a high expression level in the late fruit development of the oil palm fruit mesocarp. At the same time, it is also predicted to be the target gene of EgmiR5179, which has been identified in previous research. In this paper, we focused on the function and regulatory mechanism of the EgMADS16 gene in oil palm lipid metabolism. The results indicated that the transcription level of EgMADS16 was highest in the fourth stage, and a dual-luciferase reporter assay proved that the EgMADS16 expression level was downregulated by EgmiR5179. In both the OXEgMADS16 Arabidopsis seeds and oil palm embryonic calli, the total lipid contents were significantly decreased, but the contents of C18:0 and C18:3 in OXEgMADS16 lines were significantly increased. As expected, EgmiR5179 weakened the inhibitory effect of EgMADS16 on the oil contents in transgenic Arabidopsis plants that coexpressed EgmiR5179 and EgMADS16 (OXEgmiR5179-EgMADS16). Moreover, yeast two-hybrid and BiFC analyses suggested that there was an interaction between the EgMADS16 protein and EgGLO1 protein, which had been proven to be capable of regulating fatty acid synthesis in our previous research work. In summary, a model of the molecular mechanism by which miRNA5179 targets EgMADS16 to regulate oil biosynthesis was hypothesized, and the research results provide new insight into lipid accumulation and molecular regulation in oil palm.

scholarly journals Occurrence and Severity of Physiological Disorders of Oil Palm (Elaeis guineensis Jacq. L.) in Uganda

2020 ◽  
Vol 12 (10) ◽  
pp. 86
Author(s):  
Fred Bwayo Masika ◽  
Isaac Danso ◽  
Rossette Nangonzi ◽  
Otuba Moses Amugoli ◽  
Alex Asiimwe ◽  
...  
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Industrial Sector ◽  
Smallholder Farmer ◽  
Palm Fruit ◽  
Ecological Zones ◽  
Physiological Disorders ◽  
Adaptive Trials ◽  
Bunch Rot ◽  
Corporate Investors

In Africa, oil palm is grown in 25 countries supported by corporate investors. In Uganda, commercial oil palm cultivation began in 2005 in Bugala Islands. Seedlings were imported from countries with established breeding programs. These seedlings were grown in areas with different environmental conditions which have resulted in a number of physiological disorders. The aim of this research was to determine the major physiological disorders in oil palm fruit bunches in Uganda. The study was carried out in the adaptive trials in Kagadi, Bugiri, Buvuma and Masaka Districts and in the different smallholder farmer blocks in Kalangala District. Data was collected on bunch rot, bunch failure and uneven ripening. Sampling was carried out in oil palm plantations above five years of age. Three fields were selected from each unit and three units from each block by the help of the Agricultural Extension Officers (AEOs). Palms were randomly sampled and assessed for presence of bunch rot, bunch failure and uneven ripening symptoms. The incidence was expressed as a percentage of the total number of palms sampled while the severity of bunch rot disease was scored on a scale of 0-4. From the results, the differences in bunch rot and bunch failure in adaptive trials were statistically significant as well as across seasons (P < 0.05). Uneven ripening was not statistically significant and severity of bunch rot in the different farmer blocks in Kalangala was statistically significant (P = 0.03). Uneven ripening was high across smallholder farmer blocks in Kalangala and was statistically significant (P = 0.05) even across seasons (P < 0.05). These results are important for sensitization of farmers on management of oil palm disorders and essential for guiding policy makers and investors as the oil palm industrial sector is being developed in Uganda. This study calls for determination of water deficit at the various ecological zones and its relationship to physiological disorders as a guide for further oil palm estate development.

Effect of thiocarbamate, urea, and uracil herbicides on lipid metabolism in groundnut (Arachis hypogaea) leaves

1990 ◽  
Vol 68 (2) ◽  
pp. 567-573 ◽  
Author(s):  
R. Rajasekharan ◽  
P. S. Sastry
Keyword(s):  
Lipid Metabolism ◽  
Arachis Hypogaea ◽  
Acid Synthesis ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
The Other ◽  
Acetate Incorporation ◽  
Phosphatidic Acid Phosphatase ◽  
Choline Incorporation ◽  
Plant Lipids

The effect of thiocarbamates (S-ethyldipropylthiocarbamate and diallate), substituted ureas (monuron and diuron), and uracils (bromacil and terbacil) on lipid metabolism in groundnut (Arachis hypogaea) leaves was investigated under nonphotosynthetic conditions. The uptake of [1-14C]acetate by leaf disks was inhibited by the thiocarbamates and marginally by the substituted ureas, but not by the uracil herbicides. The uptake of [methyl-14C]choline was inhibited to a lesser extent by thiocarbamates, while the other herbicides showed a slight stimulation. The thiocarbamates almost completely inhibited uptake of [32P]orthophosphate at 1.0 mM concentration, while diuron and terbacil showed significant inhibition. [1-14C]Acetate incorporation into lipids was inhibited only by diallate. [methyl-14C]Choline incorporation into the choline phosphoglycerides was inhibited by diallate, diuron, and bromacil. The incorporation of [32P]orthophosphate into phospholipids was substantially inhibited (over 90% at 1.0 mM) by the thiocarbamates, but not by the other herbicides. [35S]Sulfate incorporation into sulfoquinovosyl diglycerides was markedly inhibited only by the thiocarbamates. Fatty acid synthesis by isolated chloroplasts was inhibited 40–85% by thiocarbamates, substituted ureas, and bromacil, but not by terbacil. The inhibitory effect of the urea derivatives was reversible, but that of thiocarbamates was irreversible. sn-Glycerol-3-phosphate acyltransferase(s) of the chloroplast and microsomal fractions were profoundly inhibited by thiocarbamates, but not by the other two groups of herbicides. Phosphatidic acid phosphatase was insensitive to all the herbicides tested.Key words: herbicides, thiocarbamates, substituted ureas, uracils, effects on plant lipids.

scholarly journals Morphogenesis of Oil Palm Fruit (Elaeis guineensis Jacq.) in Mesocarp and Endocarp Development

2013 ◽  
Vol 7 (2) ◽  
Author(s):  
Hermine Bille Ngalle ◽  
Joseph Martin Bell ◽  
Georges Franck Ngando Ebongue ◽  
Lambert Nyobe ◽  
Félix Chancelin Ngangnou ◽  
...  
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Palm Fruit ◽  
Elaeis Guineensis Jacq

scholarly journals YOUTHS’ INVOLVEMENT IN OIL PALM (ELAEIS GUINEENSIS) FRUIT PROCESSING ACTIVITIES

2021 ◽  
Vol 53 (4) ◽  
pp. 384-399
Author(s):  
Julius Olatunde AYINDE ◽  
Michael FAMAKINWA ◽  
Babatunde Opeyemi AKEREDOLU
Keyword(s):  
Oil Palm ◽  
Agricultural Development ◽  
Formal Education ◽  
Elaeis Guineensis ◽  
Sampling Procedure ◽  
Palm Fruit ◽  
Level Of Involvement ◽  
Fruit Processing ◽  
Fresh Fruit Bunches ◽  
Multistage Sampling

This study assessed the youths’ involvement in oil palm fruit processing activities in Ondo State, Nigeria. It described the socio-economic characteristics of youths involved in oil palm fruit processing activities, determined their level of involvement, examined their perception and identified constraints associated with their involvement. Multistage sampling procedure was employed to select 120 respondents from the study area. Interview schedule was employed to collect relevant data, which was analysed with SPSS software package. Descriptive statistics were used to summarise the data while inferential statistics were used to draw inference on hypothesis. The results show that majority (63.3%) were male, 95.8% had formal education with a mean age of 27.2 ± 2.7 years. Picking of fresh fruit bunches ( = 2.71), packaging ( = 2.60) and gathering of bunches ( = 2.50) were the major activities youth involved in. Higher percentage (57.9%) of the youth had favourable perception towards involvement in oil palm fruit processing activities. Lack of modern processing facilities ( =3.65) and funds/inadequate credit facilities ( = 3.65) were the prime constraints to their involvement. Number of labour (r = 0.7460; p≤0.01) and income (r = 0.601; p≤0.01) of the respondents were significantly related to youth involvement. The study concluded that youth had moderate involvement in oil palm processing activities. However, agricultural development stakeholders like government should provide adequate and functional credits facilities to these youths to encourage their involvement.

scholarly journals A RIPENESS STUDY OF OIL PALM FRESH FRUIT AT THE BUNCH DIFFERENT POSITIONS

2020 ◽  
Vol 8 (1) ◽  
pp. 9-14
Author(s):  
Rismen Sinambela
Keyword(s):  
Water Content ◽  
Oil Palm ◽  
Oil Content ◽  
Elaeis Guineensis ◽  
Soxhlet Extraction ◽  
Fresh Fruit ◽  
Test Method ◽  
Central Position ◽  
Fresh Fruit Bunch ◽  
Palm Fruit

The paper aims to study the position of the optimum oil palm ripeness at the bunch different positions. This information is essential to complete a measurement procedure to detect oil palm fresh fruit bunch (FFB) maturity so that the detection devices can directly measure the optimal mature position as a representative of the entire FFB characteristics. In this study, the oil palm FFB (Elaeis guineensis Jacq. var. tenera) with the various ripeness stages (4 until 22 weeks after anthesis) were collected and divided from three positions, i.e., proximal, central and distal. Moreover, each fruit in each of these positions was subjected to sample preparation to identify water and oil content. The water and oil content were completed based on the oven test method and the Soxhlet extraction technique, respectively. The optimum ripeness position is determined based on the lowest water content and the highest oil content. Based on the analysis, during the process of oil palm maturation occurs a decrease in water content and an increase in oil content. In addition, the average water content of palm fruit varies greatly depending on its position based on the analysis, i.e., proximal (45.38±5.62%), central (35.30±3.34%) and distal (41.98±2.57%). The average oil content of oil palm fruit in the central position is higher oil content (25.10±1.72%) compared to the proximal (10.00±0.77%) and distal position (13.77±1.22%). We suspect that the chemical content differences of palm fruit in various positions are due to the inequality of the respiration rate and ethylene production throughout FFB. In addition, overall it can be concluded that the fruit in the central FFB position has an optimal ripeness level compared to the proximal and distal position. Thus, the measurement position recommended in evaluating palm maturity is at the central position of FFB.

scholarly journals Fluorescent Mitoxantrone Hydrochloride Nanoparticles Inhibit the Malignant Behavior of Giant Cell Tumor of Bone via miR-125b/PTH1R Axis

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Baohui Su ◽  
Yanguang Yuan ◽  
Junshan Zhang ◽  
Yuezhong Li ◽  
Qihui Zhang
Keyword(s):  
Adsorption Capacity ◽  
Giant Cell Tumor ◽  
Giant Cell ◽  
Inhibitory Effect ◽  
Cell Tumor ◽  
Expression Level ◽  
Luciferase Reporter ◽  
Therapeutic Effects ◽  
Expression Levels ◽  
Malignant Behavior

Objective. To explore the therapeutic effects and mechanism of fluorescent mitoxantrone hydrochloride nanoparticles on giant cell tumor of bone. Methods. The adsorption capacity of nanoparticles to hydroxyapatite (HA), cell adsorption capacity, encapsulation rate, particle size, and potential of the nanoparticles were determined by HPLC and Zetasizer Nano ZS nanomicelle potentiometer. MTT assay was used to determine the toxicity of nanoparticles to cells. The fluorescent intensity of the nanoparticles and their location in the cells were observed under a fluorescence microscope. RT-qPCR and Western blotting were then used to measure the expression levels of miRNA, mRNA, and proteins in cells. Transwell and Annexin V-FITC/PI staining tests were used to study the cell invasion and apoptotic rate, respectively. The dual-luciferase reporter gene experiment was then carried out to verify the binding relationship between miR-125b and its predicted target. Results. ALN-FOL-MTO-NLC nanoparticles showed a stronger adsorption capacity for HA and stronger toxicity to GCTB28 cells. Compared to normal tissues, the expression level of miR-125b in giant bone tumor tissue and cells was significantly downregulated, and the expression level of miR-125b was upregulated to some extent after treatment. Overexpression of miR-125b or treatment of ALN-FOL-MTO-NLC nanoparticles can inhibit the malignant behavior of GCTB28 cells, whereas the inhibition of the expression of miR-125b can promote the malignant behavior of GCTB28 cells. The result showed that parathyroid hormone receptor 1 (PTH1R) was a downstream target gene for miR-125b. Rescue experiment showed that the treatment of GCTB28 with ALN-FOL-MTO-NLC nanoparticles while inhibiting miR-125b expression can reduce the inhibitory effect of miR-125b on the malignant behavior of GCTB28 cells, whereas upregulating the expression levels of miR-125b and PTH1R in GCTB28 cells had no significant effect on the malignant behavior of GCTB28 cells. Conclusion. ALN-FOL-MTO-NLC nanoparticles have a certain inhibitory effect on the malignant behavior of giant cell tumor of bone through the miR-125b/PTH1R molecular axis.

scholarly journals microRNA-96 promotes occurrence and progression of colorectal cancer via regulation of the AMPKα2-FTO-m6A/MYC axis

2020 ◽  
Vol 39 (1) ◽  
Author(s):  
Caifeng Yue ◽  
Jierong Chen ◽  
Ziyue Li ◽  
Laisheng Li ◽  
Jugao Chen ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Growth Cycle ◽  
Inhibitory Effect ◽  
Luciferase Reporter ◽  
Loss Of Function ◽  
Cycle Progression ◽  
Dependent Inhibition ◽  
The Relationship ◽  
Dual Luciferase Reporter Assay

Abstract Background Colorectal cancer (CRC) is one of the frequently occurred malignancies in the world. To date, several onco-microRNAs (miRNAs or miRs), including miR-96, have been identified in the pathogenesis of CRC. In the present study, we aimed to corroborate the oncogenic effect of miR-96 on CRC and to identify the specific mechanisms related to AMPKα2/FTO/m6A/MYC. Methods RT-qPCR and Western blot analysis were performed to examine the expression pattern of miR-96, AMPKα2, FTO and MYC in the clinical CRC tissues and cells. The relationship between miR-96 and AMPKα2 was then predicted using in silico analysis and identified by dual-luciferase reporter assay. Gain- or loss-of-function approaches were manipulated to evaluate the modulatory effects of miR-96, AMPKα2, FTO and MYC on cell growth, cycle progression and apoptosis. The mechanism of FTO-mediated m6A modification of MYC was analyzed via Me-RIP and PAR-CLIP analysis. The mediatory effects of miR-96 antagomir on cancerogenesis were validated in vivo. Results miR-96, FTO and MYC were upregulated, while AMPKα2 was downregulated in CRC tissues and cells. miR-96 could down-regulate AMPKα2, which led to increased expression of FTO and subsequent upregulated expression of MYC via blocking its m6A modification. This mechanism was involved in the pro-proliferative and anti-apoptotic roles of miR-96 in CRC cells. Besides, down-regulation of miR-96 exerted inhibitory effect on tumor growth in vivo. Conclusions Taken together, miR-96 antagomir could potentially retard the cancerogenesis in CRC via AMPKα2-dependent inhibition of FTO and blocking FTO-mediated m6A modification of MYC, highlighting novel mechanisms associated with colorectal cancerogenesis.

Sign in / Sign up

Export Citation Format

Share Document