scholarly journals Rubella Serosurvey Among Future Healthcare Workers

2021 ◽  
Vol 9 ◽  
Author(s):  
Andrea Trevisan ◽  
Paola Mason ◽  
Annamaria Nicolli ◽  
Stefano Maso ◽  
Chiara Bertoncello
Keyword(s):  
Infectious Disease ◽  
Antibody Response ◽  
Antibody Titer ◽  
Healthcare Workers ◽  
Herd Immunity ◽  
High Antibody ◽  
Congenital Rubella ◽  
School Of Medicine ◽  
High Antibody Titer ◽  
Very High

Objective: Rubella is a very diffusive but relatively benign infectious disease unless contracted during pregnancy, when it causes congenital rubella syndrome. The aim of this research was to determine the prevalence and titer of antirubella antibodies in a population of future healthcare workers (students at the school of medicine).Methods: The cohort consisted of 11,022 students who underwent antibody analysis after the presentation of a vaccine certificate.Results: Vaccination compliance was very high, particularly in younger students (born after 1995), reaching almost 100% (at least one dose). Unvaccinated students born before 1990 had high seropositivity (>95%), but this percentage dropped to zero among the youngest students. Variables affecting antibody titer included year of birth and sex. Considering only vaccinated students, a greater antibody response was observed if the vaccine was administered between 8 and 10 years of age. Female sex was associated with more significant (p < 0.0001) positivity and higher antibody titer after one and two doses. However, this difference appeared less consistent in relation to year of birth.Conclusions: The studied population exhibited excellent vaccination compliance, high seropositivity, and high antibody titer. Vaccine and immune coverage were higher than what is deemed necessary to achieve herd immunity.

scholarly journals Neutralizing Anti-SARS-CoV-2 Antibody Titer and Reported Adverse Effects, in a Sample of Italian Nursing Home Personnel after Two Doses of the BNT162b2 Vaccine Administered Four Weeks Apart

Vaccines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 652
Author(s):  
Alberto Modenese ◽  
Stefania Paduano ◽  
Annalisa Bargellini ◽  
Rossana Bellucci ◽  
Simona Marchetti ◽  
...  
Keyword(s):  
Nursing Home ◽  
Adverse Effects ◽  
Antibody Titer ◽  
Healthcare Workers ◽  
Vaccination Campaign ◽  
Northern Italy ◽  
High Antibody ◽  
Protective Level ◽  
High Antibody Titer ◽  
Main Factors

Background: The immunization of healthcare workers (HCWs) plays a recognized key role in prevention in the COVID-19 pandemic: in Italy, the vaccination campaign began at the end of December 2020. A better knowledge of the on-field immune response in HCWs, of adverse effects and of the main factors involved is fundamental. Methods: We performed a study on workers at a nursing home in Northern Italy, vaccinated in January–February 2021 with two doses of the BNT162b2 vaccine four weeks apart, instead of the three weeks provided for in the original manufacturer protocol. One month after the second dose, the serological titer of IgG-neutralizing anti-RBD antibodies of the subunit S1 of the spike protein of SARS-CoV-2 was determined. The socio-demographic and clinical characteristics of the subjects and adverse effects of vaccination were collected by questionnaire. Results: In all of the workers, high antibody titer, ranging between 20 and 760 times the minimum protective level were observed. Titers were significantly higher in subjects with a previous COVID-19 diagnosis. Adverse effects after the vaccine were more frequent after the second dose, but no severe adverse effects were observed. Conclusions: The two doses of the BNT162b2 vaccine, even if administered four weeks apart, induced high titers of anti-SARS-CoV-2 neutralizing IgG in all the operators included in the study.

scholarly journals High Antibody Titer against Apical Membrane Antigen-1 Is Required to Protect against Malaria in the Aotus Model

PLoS ONE ◽  
2009 ◽  
Vol 4 (12) ◽  
pp. e8138 ◽  
Author(s):  
Sheetij Dutta ◽  
JoAnn S. Sullivan ◽  
Katharine K. Grady ◽  
J. David Haynes ◽  
Jack Komisar ◽  
...  
Keyword(s):  
Antibody Titer ◽  
Apical Membrane ◽  
High Antibody ◽  
Apical Membrane Antigen 1 ◽  
Apical Membrane Antigen ◽  
High Antibody Titer

Ascending myelitis with high antibody titer to herpes simplex virus in the cerebrospinal fluid

1982 ◽  
Vol 227 (3) ◽  
pp. 187-191 ◽  
Author(s):  
M. -L. Koskiniemi ◽  
A. Vaheri ◽  
V. Manninen ◽  
P. Nikki
Keyword(s):  
Cerebrospinal Fluid ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Antibody Titer ◽  
High Antibody ◽  
High Antibody Titer ◽  
Simplex Virus

scholarly journals KEMAMPUAN NETRALISASI KEKEBALAN TUBUH INDUK MELALUI KUNING TELUR AYAM PETELUR PADA VIRUS AI (H5N1) ISOLAT LAPANGAN

2020 ◽  
Vol 6 (2) ◽  
pp. 89
Author(s):  
Agung Puji Haryanto
Keyword(s):  
Antibody Titer ◽  
Neutralization Test ◽  
Egg Yolk ◽  
Maternal Antibody ◽  
Control Group ◽  
High Antibody ◽  
Field Isolates ◽  
High Antibody Titer ◽  
Serum Neutralization Test ◽  
Group 2

Day old chick in the first week of their life got the immunity from maternal antibody / immunoglobulin trough egg yolk. Hens with high antibody titer will decended the immunoglobulin to their harvest trough egg yolk. The research studied the capability of antibody anti H5N1 from egg yolk to neutralized H5N1 AIV.  Twenty hens were devided to five group, one group as  a control group were unvaccinated group and four other groups were vaccinated with H5N1 AI inactive vaccine produced by several  Indonesia vaccine company. Four H5N1 AI inactive vaccines code VS1, VC1, VV1 and VM1 were vaccinated twice to group 2, 3, 4 and 5. A week after second vaccination the egg were collected and analyzed the antibody titer against H5N1 AIV  by hemagglutination test using H5N1 AIV field isolates as standard virus (NG and LW)). The egg yolk were contain high antibody titer (above 26) collected and tested against H5N1 AIV field isolates NG and LW by serum neutralization test.  The result showed that H5N1 AIV NG isolate were able to neutralized by antibody anti H5N1 AIV from egg yolk produced by hens vaccinated with VS1, VC1, VV1 and VM1, but LW isolate were able to neutralized by showed that H5N1 AIV NG isolate were able to neutralized by antibody anti H5N1 AIV from egg yolk produced by hens vaccinated with VS1, VC1, and VM1.  It’s concluded that hens were vaccinated with H5N1 AI inactive vaccine were able to protect their off spring against H5N1 AIV from the field by transferred maternal antibody trough the egg yolk with titer above 26.

scholarly journals Development of the scheme of obtaining antibodies to the ribonucleoprotein of attenuated rabies virus

2019 ◽  
pp. 3-8 ◽  
Author(s):  
Yu. K. Gavrilova ◽  
S. V. Generalov ◽  
M. N. Kireev ◽  
N. A. Sharapova ◽  
E. G. Abramova ◽  
...  
Keyword(s):  
Cell Culture ◽  
Rabies Virus ◽  
Antibody Titer ◽  
Colloidal Gold ◽  
Target Component ◽  
High Antibody ◽  
Experimental Animals ◽  
Colloidal Gold Nanoparticles ◽  
High Antibody Titer ◽  
Dot Immunoassay

Aim. Isolation of ribonucleoprotein (RNP) of an attenuated rabies virus, develop schemes for immunizing animals with RNP-based preparations and determine the most effective scheme that allows obtaining serum with a high antibody titer to the RNP.Materials and methods. We used the transplantable cell line Vero, the strain rabies virus «Moscow 3253 Vero», adapted for reproduction on Vero, rabbits of the chinchilla breed. In order to obtain serums containing antibodies to the RNP of the rabies virus, experimental schemes have been proposed for immunizing animals with RNP, including with adjuvants: polyoxidonium and colloidal gold. The dynamics of the accumulation of antibodies to the RNP of the rabies virus in the blood serum of experimental animals was studied by dot-immunoassay.Results. The target component (RNP of the rabies virus) was isolated directly from the cytoplasm of the Vero cell culture infected with the rabies virus according to the modified M. Dastkhosh (2014) method, lyophilized and used in the development of preparations for immunizing experimental animals. In the study of the dynamics of the formation of antibodies to RNP of the rabies virus by the method of dot-immunoassay, the effectiveness of an adjuvant is established — colloidal gold nanoparticles ranging in size from 15 to 17 nm, the use of which makes it possible to increase the antibody titer by 2 times.Conclusion. The results obtained are of interest for further research related to the design of diagnostic products and the development of methodological techniques using such preparations.

Vaccination against follistatin affects reproductive potential in cycling gilts

2000 ◽  
Vol 80 (2) ◽  
pp. 337-342
Author(s):  
C. R. Christensen ◽  
M. J. Redmond ◽  
B. Laarveld
Keyword(s):  
Key Words ◽  
Litter Size ◽  
Antibody Titer ◽  
High Titer ◽  
Reproductive Potential ◽  
High Antibody ◽  
High Antibody Titer ◽  
Antibody Titers

Primiparous sows (gilts) were actively vaccinated against follistatin in an attempt to modify litter size. Forty-seven gilts were vaccinated four times against a recombinant porcine follistatin (FS) or a sham vaccine (CTL) and were allowed to mature naturally prior to breeding. At breeding, FS antibody titers ranged from 0 to 1:6400 in the FS vaccinated gilts, and were not detectable in the CTL gilts. Overall, follistatin vaccination did not affect the total number of pigs born live (FS = 10.9 ± 0.5, CTL = 10.3 ± 0.4), stillborn (FS = 0.4 ± 0.1, CTL = 0.4 ± 0.2) or mummified (FS = 0.1 ± 0.1, CTL = 0.3 ± 0.1). However, separation of the FS vaccinated gilts into low (≤1:400, n = 16) and high (>1:400, n = 7) titer groups revealed significant differences in piglets born alive (FS high titer = 12.9 ± 0.9, FS low titer = 10.0 ± 0.5: P = 0.01) and total number of piglets born (FS high titer = 13.0 ± 0.8, FS low titer = 10.8 ± 0.6: P = 0.08). This study shows that vaccination of gilts against follistatin increased litter size in those gilts which achieved a high antibody titer to follistatin. Key words: Swine, follistatin, immunoneutralization, fecundity, litter size

scholarly journals Serum Antibody Response Comparison and Adverse Reaction Analysis in Healthcare Workers Vaccinated with the BNT162b2 or ChAdOx1 COVID-19 Vaccine

Vaccines ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1379
Author(s):  
Sung-Hee Lim ◽  
Seong-Hyeok Choi ◽  
Bora Kim ◽  
Ji-Youn Kim ◽  
Young-Sok Ji ◽  
...  
Keyword(s):  
Adverse Events ◽  
Antibody Response ◽  
Healthcare Workers ◽  
Serum Antibody ◽  
Neutralizing Antibody ◽  
And Gender ◽  
Gender Categories ◽  
Antibody Levels ◽  
Very High

The COVID-19 pandemic is changing rapidly and requires different strategies to maintain immunization. In Korea, different COVID-19 vaccines are recommended and available for various populations, including healthcare workers (HCWs) at high risk of SARS-CoV-2 infection. We plan to evaluate the adverse events (AEs) and immunogenicity of the BNT162b2 and ChAdOx1 vaccines in HCWs at a single center. This cohort study included HCWs fully vaccinated with either BNT162b2 or ChAdOx1. Blood samples were taken eight weeks after the second vaccination with both COVID-19 vaccines and six months after the second vaccination from participants with the BNT162b2 vaccine. The primary endpoint for immunogenicity was the serum neutralizing antibody responses eight weeks after vaccination. The secondary endpoint was the incidence of various AEs within 28 days of each vaccination. Between 16 March and 23 June 2021, 115 participants were enrolled (65 in the ChAdOx1 group and 50 in the BNT162b2 group). Significantly higher surrogate virus neutralization test (sVNT) inhibition was observed in participants vaccinated with two doses of BNT162b2 (mean (SD) 91.4 (9.68)%) than in those vaccinated with ChAdOx1 (mean (SD) 73.3 (22.57)%). The effectiveness of the BNT162b2 vaccine was maintained across all age and gender categories. At six months after the second dose, serum antibody levels declined significantly in the BNT162b2 group. The main adverse events, including fever, myalgia, fatigue, and headache, were significantly higher in the ChAdOx1 group after the first dose, whereas, after the second dose, those AEs were significantly higher in the BNT162b2 group (p < 0.05). Two doses of either the ChAdOx1 or the BNT162b2 COVID-19 vaccine resulted in very high seropositivity among the HCWs at our center. The quality of the antibody response, measured by sVNT inhibition, was significantly better with the BNT162b2 vaccine than with the ChAdOx1 vaccine. There was no significant association between neutralizing antibody response and AE after each vaccination in our cohort.

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