scholarly journals Asian Admixture in European Echinococcus multilocularis Populations: New Data From Poland Comparing EmsB Microsatellite Analyses and Mitochondrial Sequencing

2021 ◽  
Vol 7 ◽  
Author(s):  
Gérald Umhang ◽  
Jenny Knapp ◽  
Marion Wassermann ◽  
Vanessa Bastid ◽  
Carine Peytavin de Garam ◽  
...  
Keyword(s):  
North America ◽  
Echinococcus Multilocularis ◽  
Nuclear Genome ◽  
Microsatellite Analysis ◽  
Northern Eurasia ◽  
Mitochondrial Haplotype ◽  
Red Foxes ◽  
Asian Origin ◽  
Eastern European Countries ◽  
The Impact

The cestode Echinococcus multilocularis is the causative agent of a severe zoonotic disease: alveolar echinococcosis (AE). The parasite is distributed over a vast area in northern Eurasia and North America, but the impact of AE on human health is highly uneven between different regions. One hypothetical reason for this difference in virulence may be the genetic structure of E. multilocularis which—based on mitochondrial sequences and EmsB microsatellite profiles—forms four distinct clades. These clades correspond approximately to their continents of origin: Asia, Europe, and North America, with a fourth clade apparently restricted to Mongolia and neighboring regions, even though this clade has not yet been described by EmsB genotyping. However, there are various records of genetic variants from the “wrong” region, e.g., “European” haplotypes in Western Canada, which may be the result of introduction or natural migration of host animals. One such example, prompting this study, is the recent record of an “Asian” mitochondrial haplotype in worms from foxes in Poland. At the time, this could not be confirmed by EmsB microsatellite analysis, a method that has proven to possess greater discriminatory power with the E. multilocularis nuclear genome than sequencing of mitochondrial markers. Therefore, worms collected from foxes in Poland were examined both by EmsB analysis and sequencing of the full mitochondrial cox1 gene in order to allocate the samples to the European or Asian cluster. Based on EmsB analyses of 349 worms from 97 Polish red foxes, 92% of the worms clearly showed “European-type” EmsB profiles, but 27 worms (8%) from seven foxes showed profiles that clustered with samples of Asian origin. According to cox1 sequences, a total of 18 worms from 8 foxes belonged to the Asian cluster of haplotypes. The two methods did not fully agree: only 13 worms from three foxes belonged to Asian clusters by both EmsB and cox1, whereas 18 worms from nine foxes belonged to different clusters, according to each marker. Cross-fertilization between worms of Asian origin and those from the European Polish population may explain these conflicting results. The presence of clearly Asian elements in the Polish E. multilocularis population could be the result of introduction of E. multilocularis with host animals (e.g., domestic dogs), or the migration of foxes. In the absence of genetic data from eastern European countries, especially those bordering Poland, it cannot be concluded whether this Asian admixture is typical for a larger area toward central/eastern Europe, or the Polish parasite population is the western extreme of a gradient where both European and Asian elements mingle. Further studies are needed on this subject, preferably using both mitochondrial sequencing and EmsB microsatellite analysis.

scholarly journals Insights into the Mitochondrial and Nuclear Genome Diversity of Two High Yielding Strains of Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 825
Author(s):  
Clara Heumann-Kiesler ◽  
Vera Sommerfeld ◽  
Hanna Iffland ◽  
Jörn Bennewitz ◽  
Markus Rodehutscord ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Genetic Background ◽  
Laying Hens ◽  
Nuclear Genome ◽  
Nuclear Interaction ◽  
Mitochondrial Haplotype ◽  
Essential Components ◽  
Different Strains ◽  
Eukaryotic Organisms ◽  
The Impact

Mitochondria are essential components of eukaryotes as they are involved in several organismic key processes such as energy production, apoptosis and cell growth. Despite their importance for the metabolism and physiology of all eukaryotic organisms, the impact of mitochondrial haplotype variation has only been studied for very few species. In this study we sequenced the mitochondrial genome of 180 individuals from two different strains of laying hens. The resulting haplotypes were combined with performance data such as body weight, feed intake and phosphorus utilization to assess their influence on the hens in five different life stages. After detecting a surprisingly low level of genetic diversity, we investigated the nuclear genetic background to estimate whether the low mitochondrial diversity is representative for the whole genetic background of the strains. Our results highlight the need for more in-depth investigation of the genetic compositions and mito-nuclear interaction in individuals to elucidate the basis of phenotypic performance differences. In addition, we raise the question of how the lack of mitochondrial variation developed, since the mitochondrial genome represents genetic information usually not considered in breeding approaches.

scholarly journals Mathematical Global Dynamics and Control Strategies on Echinococcus multilocularis Infection

2019 ◽  
Vol 2019 ◽  
pp. 1-17 ◽  
Author(s):  
A. S. Hassan ◽  
J. M. W. Munganga
Keyword(s):  
Echinococcus Multilocularis ◽  
Reproduction Number ◽  
Positive Impact ◽  
Control Strategies ◽  
Rank Correlation ◽  
Global Dynamics ◽  
Red Foxes ◽  
Local And Global Dynamics ◽  
The Impact ◽  
Environmental Disinfection

Echinococcus multilocularis, a major cause of echinococcosis in human, is a parasitic sylvatic disease between two major hosts in a predator-prey relation. A new model for the transmission dynamics of Echinococcus multilocularis in the population of red foxes and voles with environment as a source of infection is formulated and rigorously analyzed. The model is used to access the impact of treatment on red foxes and environmental disinfection as control strategies on the disease dynamics. The control reproduction number is computed and is used to rigorously prove the local and global dynamics of models’ equilibria. Using available data on Echinococcus, elasticity indices and partial rank correlation coefficients of control reproduction number and cumulative new cases in red foxes and voles are computed. Parameters that have high influence locally and globally are identified. Numerical experiments indicate that administering disinfection of environment only induces more positive impact than applying treatment only on red foxes in controlling the infection. Generally, interventions towards treating red foxes and environmental disinfection could be sufficient in tackling transmission of disease in the populations.

scholarly journals Deep amplicon sequencing highlights low intra-host genetic variability of Echinococcus multilocularis and high prevalence of the European-type haplotypes in coyotes and red foxes in Alberta, Canada

2021 ◽  
Vol 15 (5) ◽  
pp. e0009428
Author(s):  
Maria A. Santa ◽  
Andrew M. Rezansoff ◽  
Rebecca Chen ◽  
John S. Gilleard ◽  
Marco Musiani ◽  
...  
Keyword(s):  
North America ◽  
Relative Abundance ◽  
Echinococcus Multilocularis ◽  
Population Expansion ◽  
Amplicon Sequencing ◽  
Intraspecific Diversity ◽  
Mixed Infections ◽  
Western Canada ◽  
Red Foxes ◽  
The North

Echinococcus multilocularis (Em) is a zoonotic parasite considered a global emergent pathogen. Recent findings indicate that the parasite is expanding its range in North America and that European-type haplotypes are circulating in western Canada. However, genetic analyses are usually conducted only on a few parasites out of thousands of individuals within each definitive host, likely underestimating the prevalence of less common haplotypes. Moreover, mixed infections with several mtDNA haplotypes in the same host have been reported, but their relative abundance within the host was never estimated. We aimed to 1) estimate the frequency of co-infections of different Em haplotypes in coyotes (Canis latrans) and red foxes (Vulpes vulpes) from western Canada and their relative abundance within the definitive hosts, 2) detect less prevalent haplotypes by sampling a larger proportion of the parasite subpopulation per host, and 3) investigate differences in the distribution of Em haplotypes in these main definitive hosts; foxes and coyotes. We extracted DNA from ~10% of the worm subpopulation per host (20 foxes and 47 coyotes) and used deep amplicon sequencing (NGS technology) on four loci, targeting the most polymorphic regions from the mitochondrial genes cox1 (814 bp), nad1 (344 bp), and cob (387 bp). We detected the presence of mixed infections with multiple Em haplotypes and with different Echinococcus species including Em and E. granulosus s.l. genotypes G8/G10, low intraspecific diversity of Em, and a higher abundance of the European-type haplotypes in both hosts. Our results suggest a population expansion of the European over the North American strain in Alberta and a limited distribution of some European-type haplotypes. Our findings indicate that deep amplicon sequencing represents a valuable tool to characterize Em in multiple hosts, to assess the current distribution and possible origins of the European strain in North America. The potential use of next-generation sequencing technologies is particularly important to understand the patterns of geographic expansion of this parasite.

scholarly journals Genetic diversity of Echinococcus multilocularis in red foxes in Poland: the first report of a haplotype of probable Asian origin

2017 ◽  
Vol 64 ◽  
Author(s):  
Jacek Karamon ◽  
Krzysztof Stojecki ◽  
Malgorzata Samorek-Pierog ◽  
Ewa Bilska-Zajac ◽  
Miroslaw Rozycki ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Echinococcus Multilocularis ◽  
Red Foxes ◽  
Asian Origin ◽  
First Report

Pentecostalism and African diaspora : a case study of the Redeemed Christian Church of God (RCCG), in North America

2020 ◽  
Vol 1 (2) ◽  
pp. 77-92
Author(s):  
Rotimi Williams Omotoye
Keyword(s):  
United States ◽  
North America ◽  
United States Of America ◽  
The United States ◽  
Church Of God ◽  
Sources Of Information ◽  
Christian Church ◽  
Secondary Sources ◽  
The Impact ◽  
The Church

Pentecostalism as a new wave of Christianity became more pronounced in 1970's and beyond in Nigeria. Since then scholars of Religion, History, Sociology and Political Science have shown keen interest in the study of the Churches known as Pentecostals because of the impact they have made on the society. The Redeemed Christian Church of God (RCCG) was established by Pastor Josiah Akindayomi in Lagos,Nigeria in 1952. After his demise, he was succeeded by Pastor Adeboye Adejare Enock. The problem of study of this research was an examination of the expansion of the Redeemed Christian Church of God to North America, Caribbean and Canada. The missionary activities of the church could be regarded as a reversed mission in the propagation of Christianity by Africans in the Diaspora. The methodology adopted was historical. The primary and secondary sources of information were also germane in the research. The findings of the research indicated that the Redeemed Christian Church of God was founded in North America by Immigrants from Nigeria. Pastor Adeboye Enock Adejare had much influence on the Church within and outside the country because of his charisma. The Church has become a place of refuge for many immigrants. They are also contributing to the economy of the United States of America. However, the members of the Church were faced with some challenges, such as security scrutiny by the security agencies. In conclusion, the RCCGNA was a denomination that had been accepted and embraced by Nigerians and African immigrants in the United States of America.

scholarly journals Anthropogenic climate change has changed frequency of past flood during 2010-2013

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Yukiko Hirabayashi ◽  
Haireti Alifu ◽  
Dai Yamazaki ◽  
Yukiko Imada ◽  
Hideo Shiogama ◽  
...  
Keyword(s):  
Climate Change ◽  
South America ◽  
Radiative Forcing ◽  
Water Cycle ◽  
Flood Frequency ◽  
Anthropogenic Climate Change ◽  
Climate Change Signal ◽  
Northern Eurasia ◽  
Large Ensemble ◽  
The Impact

AbstractThe ongoing increases in anthropogenic radiative forcing have changed the global water cycle and are expected to lead to more intense precipitation extremes and associated floods. However, given the limitations of observations and model simulations, evidence of the impact of anthropogenic climate change on past extreme river discharge is scarce. Here, a large ensemble numerical simulation revealed that 64% (14 of 22 events) of floods analyzed during 2010-2013 were affected by anthropogenic climate change. Four flood events in Asia, Europe, and South America were enhanced within the 90% likelihood range. Of eight snow-induced floods analyzed, three were enhanced and four events were suppressed, indicating that the effects of climate change are more likely to be seen in the snow-induced floods. A global-scale analysis of flood frequency revealed that anthropogenic climate change enhanced the occurrence of floods during 2010-2013 in wide area of northern Eurasia, part of northwestern India, and central Africa, while suppressing the occurrence of floods in part of northeastern Eurasia, southern Africa, central to eastern North America and South America. Since the changes in the occurrence of flooding are the results of several hydrological processes, such as snow melt and changes in seasonal and extreme precipitation, and because a climate change signal is often not detectable from limited observation records, large ensemble discharge simulation provides insights into anthropogenic effects on past fluvial floods.

scholarly journals Is there an imbalance in the supply and demand for universal accessibility knowledge? Twenty years of UAIS papers viewed through the lens of WCAG

2021 ◽  
Author(s):  
Frode Eika Sandnes
Keyword(s):  
Latin America ◽  
North America ◽  
Supply And Demand ◽  
Research Focus ◽  
Universal Accessibility ◽  
Geographical Regions ◽  
Research Profile ◽  
The Impact ◽  
Over Time

AbstractPurpose: Some universal accessibility practitioners have voiced that they experience a mismatch in the research focus and the need for knowledge within specialized problem domains. This study thus set out to identify the balance of research into the main areas of accessibility, the impact of this research, and how the research profile varies over time and across geographical regions. Method: All UAIS papers indexed in Scopus were analysed using bibliometric methods. The WCAG taxonomy of accessibility was used for the analysis, namely perceivable, operable, and understandable. Results: The results confirm the expectation that research into visual impairment has received more attention than papers addressing operable and understandable. Although papers focussing on understandable made up the smallest group, papers in this group attracted more citations. Funded research attracted fewer citations than research without funding. The breakdown of research efforts appears consistent over time and across different geographical regions. Researchers in Europe and North America have been active throughout the last two decades, while Southeast Asia, Latin America, and Middle East became active in during the last five years. There is also seemingly a growing trend of out-of-scope papers. Conclusions: Based on the findings, several recommendations are proposed to the UAIS editorial board.

scholarly journals Does a National Innovation System Encourage Sustainability? Lessons from the Construction Industry in Serbia

2021 ◽  
Vol 13 (7) ◽  
pp. 3591
Author(s):  
Marija Mosurović Ružičić ◽  
Mirjana Miletić ◽  
Marina Dobrota
Keyword(s):  
Energy Efficiency ◽  
Construction Industry ◽  
Innovation System ◽  
Business Environment ◽  
National Innovation System ◽  
Eastern European ◽  
System Perspective ◽  
Modern Business ◽  
Eastern European Countries ◽  
The Impact

Influences from the modern business environment indicate the need for the incorporation of sustainability concepts from an innovation system perspective. In the presented research, we emphasize the energy efficiency concept within the frame of sustainability and innovation. The aim of this research was to underline and explore the relationships between innovation, energy efficiency, and sustainability in the construction industry. To answer the research questions, a questionnaire was created to explore the impact of the energy efficiency certification process on the innovation behavior of construction industry enterprises in Serbia. The results show that energy efficiency has supported innovation, and that there exists a relationship between sustainability and innovativeness in the construction industry. Applying energy efficiency passports has influenced the co-operation of enterprises in the construction sector and other actors in the national innovation system in Serbia. The innovation concept demonstrates that enterprises in the construction industry should be observed as a part of the wider picture—the national innovation system. In turn, the specific context of a particular national innovation system should be seen within the wider picture of national innovation systems of Central and Eastern European Countries (CEECs).

scholarly journals A Link between the Hiatus in Global Warming and North American Drought

2015 ◽  
Vol 28 (9) ◽  
pp. 3834-3845 ◽  
Author(s):  
Thomas L. Delworth ◽  
Fanrong Zeng ◽  
Anthony Rosati ◽  
Gabriel A. Vecchi ◽  
Andrew T. Wittenberg
Keyword(s):  
Global Warming ◽  
North America ◽  
Surface Temperature ◽  
North American ◽  
Radiative Forcing ◽  
Tropical Pacific ◽  
Global Warming Hiatus ◽  
Warming Hiatus ◽  
The Impact ◽  
The Tropical Pacific

Abstract Portions of western North America have experienced prolonged drought over the last decade. This drought has occurred at the same time as the global warming hiatus—a decadal period with little increase in global mean surface temperature. Climate models and observational analyses are used to clarify the dual role of recent tropical Pacific changes in driving both the global warming hiatus and North American drought. When observed tropical Pacific wind stress anomalies are inserted into coupled models, the simulations produce persistent negative sea surface temperature anomalies in the eastern tropical Pacific, a hiatus in global warming, and drought over North America driven by SST-induced atmospheric circulation anomalies. In the simulations herein the tropical wind anomalies account for 92% of the simulated North American drought during the recent decade, with 8% from anthropogenic radiative forcing changes. This suggests that anthropogenic radiative forcing is not the dominant driver of the current drought, unless the wind changes themselves are driven by anthropogenic radiative forcing. The anomalous tropical winds could also originate from coupled interactions in the tropical Pacific or from forcing outside the tropical Pacific. The model experiments suggest that if the tropical winds were to return to climatological conditions, then the recent tendency toward North American drought would diminish. Alternatively, if the anomalous tropical winds were to persist, then the impact on North American drought would continue; however, the impact of the enhanced Pacific easterlies on global temperature diminishes after a decade or two due to a surface reemergence of warmer water that was initially subducted into the ocean interior.

Potential remedy against Echinococcus multilocularis in wild red foxes using baits with anthelmintic distributed around fox breeding dens in Hokkaido, Japan

Parasitology ◽  
2002 ◽  
Vol 125 (2) ◽  
pp. 119-129 ◽  
Author(s):  
H. TSUKADA ◽  
K. HAMAZAKI ◽  
S. GANZORIG ◽  
T. IWAKI ◽  
K. KONNO ◽  
...  
Keyword(s):  
Echinococcus Multilocularis ◽  
Red Fox ◽  
Red Foxes ◽  
Clethrionomys Rufocanus ◽  
Efficient Control ◽  
Positive Rate ◽  
Potential Remedy ◽  
Patent Infection

The effect of bait-delivered anthelmintic to reduce the prevalence of Echinococcus multilocularis in wild red foxes was evaluated in Koshimizu, in the eastern part of Hokkaido, Japan. The study area (200 km2) was divided into baited and non-baited sections. The anthelmintic baits were distributed around fox den sites in the baited section every month for 13 months. After 1 year of the anthelmintic bait distribution, the prevalence of E. multilocularis in foxes, evaluated either by the parasite egg examination (from 27.1 to 5.6%) or coproantigen ELISA (from 59.6 to 29.7%), decreased in the baited section contrasting to that in the non-baited section (parasite egg: from 18.8 to 24.2%; ELISA: from 41.9 to 45.8%). The prevalence of E. multilocularis in grey red-backed vole Clethrionomys rufocanus, caught around fox dens, born after bait distribution also decreased and was significantly lower than that in non-baited section. However, within the study periods, the coproantigen-positive rate in fox faeces sporadically increased, while egg-positive rate constantly decreased. Since coproantigen ELISA can detect pre-patent infection, this observation indicates that reinfection pressure in the baited section was still high even after the 13 months of anthelmintic bait distribution. Therefore, the bait distribution longer than our study period is required for the efficient control of E. multilocularis in wild red fox population.

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