scholarly journals Plasma Proteomics Characteristics of Subclinical Vitamin E Deficiency of Dairy Cows During Early Lactation

2021 ◽  
Vol 8 ◽  
Author(s):  
Weidong Qian ◽  
Hongyi Yu ◽  
Cuiyu Zhang ◽  
Hongyou Zhang ◽  
Shixin Fu ◽  
...  
Keyword(s):  
Vitamin E ◽  
Western Blot ◽  
Dairy Cows ◽  
Signaling Pathway ◽  
Post Partum ◽  
Healthy Controls ◽  
Vitamin E Deficiency ◽  
Potential Biomarker ◽  
Plasma Proteomics ◽  
Post Partum Period

Vitamin E (VE) is an essential fat-soluble nutrient for dairy cows. Vitamin E deficiency leads to immune suppression and oxidative stress and increases the susceptibility of cows to reproductive disorders in the early post-partum period. However, studies on plasma proteomics of VE deficiency have not been reported so far. Therefore, the purpose of this study was to understand the changes of blood protein profile in cows with subclinical VE deficiency in the early post-partum period. In this study, plasma protein levels of 14 healthy cows (>4 μg/ml α-tocopherol) and 13 subclinical VE-deficient cows (2–3 μg/ml α-tocopherol) were analyzed by tandem mass tag (TMT). The results showed that there were 26 differentially expressed proteins (DEPs) in the plasma of cows with subclinical VE deficiency compared with healthy controls. Twenty-one kinds of proteins were downregulated, and five kinds were upregulated, among which eight proteins in protein–protein interactions (PPI) network had direct interaction. These proteins are mainly involved in the MAPK signaling pathway, pantothenic acid and coenzyme A (CoA) biosynthesis, PPAR signaling pathway, and glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The top four DEPs in PPI (APOC3, APOC4, SAA4, PHLD) and one important protein (VNN1) by literature review were further verified by ELISA and Western blot. The expression levels of APOC3, VNN1, and SAA4 were significantly lower than those of healthy controls by ELISA. VNN1 was significantly lower than those of healthy controls by Western blot. VNN1 is closely related to dairy cow subclinical VE deficiency and can be a potential biomarker. It lays a foundation for further research on the lack of pathological mechanism and antioxidative stress of VE.

scholarly journals AKNA Is a Potential Prognostic Biomarker in Gastric Cancer and Function as a Tumor Suppressor by Modulating EMT-Related Pathways

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Gang Wang ◽  
Dan Sun ◽  
Wenhui Li ◽  
Yan Xin
Keyword(s):  
Gastric Cancer ◽  
Cell Adhesion ◽  
Tumor Suppressor ◽  
Western Blot ◽  
Signaling Pathway ◽  
Gene Set Enrichment Analysis ◽  
Mesenchymal Transition ◽  
Qrt Pcr ◽  
Potential Biomarker ◽  
Cell To Cell Adhesion

The AT-hook transcription factor, AKNA, is a nuclear protein that affects a few physiological and pathological processes including cancer. Here, we investigated the role of AKNA in gastric cancer (GC). By using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot assays, AKNA was found deregulated in both GC cell lines and 32 paired GC tissues. Subsequently, Kaplan-Meier analysis and clinicopathological analysis were conducted using both 32 GC cases’ data above and RNA-Seq data of AKNA in 354 GC patients and the corresponding clinical-pathological data obtained from The Cancer Genome Atlas (TCGA), and AKNA expression was found closely related to location, metastasis, and TNM staging of GC. Then, the potential molecular mechanisms of AKNA in GC were explored by gene set enrichment analysis (GSEA), qRT-PCR, and Western blot assays. AKNA was found to be a hub gene related to homotypic cell to cell adhesion, regulation of cell to cell adhesion, leukocyte cell to cell adhesion, and regulation of T cell proliferation in GC. GO analysis revealed that AKNA involved in the regulation of epithelial-mesenchymal transition (EMT)-related pathways including chemokine signaling pathway, cytokine to cytokine receptor interaction, cell adhesion molecules, and jak-stat signaling pathway in GC. To explore the regulation of AKNA expression, Targetscan and TargetMiner were used to predict the possible miRNA which targeted AKNA and found the expression of AKNA was negatively correlated to miR-762 which could be sponged by circTRNC18. In conclusion, AKNA could function as a tumor suppressor by modulating EMT-related pathways in GC. The expression of AKNA might be regulated by circTRNC18/miR-762 axis. AKNA could serve as a potential biomarker and an effective target for GC diagnosis and therapy.

scholarly journals Resumption of follicular activity in the early post-partum period of dairy cows

Reproduction ◽  
1990 ◽  
Vol 88 (2) ◽  
pp. 569-579 ◽  
Author(s):  
J. D. Savio ◽  
M. P. Boland ◽  
N. Hynes ◽  
J. F. Roche
Keyword(s):  
Dairy Cows ◽  
Post Partum ◽  
Post Partum Period ◽  
Follicular Activity

Studies on plasma progesterone concentrations and fertility in Friesland dairy cows during the post-partum period

1981 ◽  
Vol 97 (3) ◽  
pp. 649-654 ◽  
Author(s):  
D. H. Holness ◽  
G. W. Sprowson ◽  
Carole Sheward ◽  
Amanda Geel
Keyword(s):  
Dairy Cows ◽  
Early Pregnancy ◽  
Post Partum ◽  
Ovarian Activity ◽  
Plasma Progesterone ◽  
Low Concentrations ◽  
The Mean ◽  
Post Partum Period ◽  
Embryo Loss

SUMMARYPlasma progesterone concentrations were monitored in 69 lactating Friesland dairy cows during the post-partum period. Cyclic ovarian activity resumed in most cows within 40 days of calving. Forty-eight per cent of cows remained pregnant to their first insemination, 41% returned to oestrus before day 30 after insemination, and a further 11% returned to oestrus between days 30 and 88 after insemination.The mean of progesterone values on days 11 to 4 before insemination tended to be higher in cows that conceived than in cows that did not conceive. These differences were signficant on day –8 (P <0·05) and on days –8, –7, –6 and –5 combined (P <0·01). Cows in which the concentration of progesterone in plasma did not rise above 4·5 ng/ml during this period did not conceive. After insemination, mean progesterone concentrations between pregnant and non-pregnant cows diverged after day +8. Relatively low concentrations of plasma progesterone (< 1 ng/ml) up to day +8 after insemination did not preclude conception. There was no indication that embryo loss between days +30 and +88 after insemination was related to an insufficiency of ovarian progesterone during early pregnancy.

scholarly journals Chronic Vitamin E Deficiency in Rural Bangladeshi Women

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 648-648
Author(s):  
Sabi Gurung ◽  
Kerry Jean Schulze ◽  
Abu Ahmed Shamim ◽  
Saijuddin Shaikh ◽  
Hasmot Ali ◽  
...  
Keyword(s):  
Vitamin E ◽  
Early Pregnancy ◽  
Post Partum ◽  
Reproductive Age ◽  
Rural Setting ◽  
Women Of Reproductive Age ◽  
Vitamin E Deficiency ◽  
Tocopherol Concentration ◽  
Pregnancy And Postpartum ◽  
Cluster Randomized

Abstract Objectives Vitamin E deficiency (VED) appears to be common in rural areas of South Asia, with prevalence of ≥ 50% reported among women of reproductive age. Long term deficiency may impart neurological damage, but little information exists about persistence of VED. We provide initial estimates of chronic VED (CVED) in women of reproductive age living in Gaibandha District, a typical rural setting in NW Bangladesh. Methods We assessed α-tocopherol concentrations by HPLC in early pregnancy and 3-month postpartum (PP) plasma samples of women participating in biochemical sub-studies of JiVitA-1 (n = 2,319), a cluster-randomized, placebo-controlled, weekly vitamin A or β-carotene supplementation trial from 2001–7, and JiVitA-3 (N = 2,073), a cluster-randomized, daily multiple micronutrient (MMS) versus iron-folic acid (IFA) supplementation trial from 2008–12. VED was conventionally defined by an α-tocopherol concentration &lt; 12 μmol/L and CVED as α-tocopherol &lt; 12 μmol/L at both early pregnancy and postpartum assessments, an interval spanning a median (IQR) of 9.4 (2.8) months. Results Across both trials combined (N = 4,392), the mean ± SD plasma α-tocopherol concentration and prevalence of VED in early pregnancy and at 3 months post-partum was 11.28 ± 4.07 and 10.86 ± 4.41 μmol/L, and 53.5% and 42.2%, respectively. In JiVitA-3, the post-partum prevalence of VED among women not previously receiving MMS (containing 15 mg of vitamin E as all-rac-α-tocopheryl acetate) was 57.5% (vs 42.5% among MMS recipients, p &lt; 0.001). The prevalence of CVED among women in both trials combined was 32.0% (34.45% in JiVitA-1 and 26.65% in JiVitA-3, IFA group only). Across a maternal age range of 11–43 [median: 20 (IQR: 8) years, risk of CVED decreased comparably per year of age in the JiVitA-1 [OR = 0.94 (95% CI: 0.93–0.95)] and JiVitA-3 [OR = 0.93 (95% CI:0.92–0.95)] trials. Conclusions In rural NW Bangladesh, where over half of women enter pregnancy vitamin E-deficient, about one-third are also deficient postpartum, ∼10 months later, providing a provisional estimate of chronic maternal vitamin E deficiency in a South Asian setting. Funding Sources Bill & Melinda Gates Foundation (Grant GH614, OPP1141435) and US Agency for International Development (AID (HRN-A-00–97-00015–00).

scholarly journals Behavioural phenomena related to normal and difficult deliveries in dairy cows.

1987 ◽  
Vol 35 (2) ◽  
pp. 87-101
Author(s):  
J. Metz ◽  
J.H.M. Metz
Keyword(s):  
Dairy Cows ◽  
Post Partum ◽  
Fetal Membranes ◽  
Final Phase ◽  
Recumbent Position ◽  
Black And White ◽  
Spontaneous Expulsion ◽  
Post Partum Period ◽  
Behavioural Aspects

Behavioural aspects of parturition, as well as the behaviour of cows and calves in the earliest post-partum period were studied in a group of 75 Polish Black and White cows. Distinction was made between unassisted deliveries, deliveries that required a little help (traction by not more than 2 persons) and difficult deliveries, requiring veterinary help. Of the cows 14% gave birth without prior behavioural signs of an approaching delivery, 72% manifested clear signs of preparturient unrest and 70% showed outflow of allantoic fluids before the calf appeared. A large variation was seen in the timing of these preparatory phases. Prolonged unrest, a cessation of labour and no appearance of the calf's feet were the clearest signs that a difficult delivery would follow. After the appearance of the feet, only 48% of the cows were able to expell the fetus within 1 h. Spontaneous expulsion of fetal membranes occurred characteristically within 12 h after parturition. Of the cows whose deliveries were unaided or aided, 86 were in recumbent position during the final phase of expulsion of fetus. The majority (64%) of cows with difficult delivery were standing. Cows separated from their calves lie down earlier after calving than those kept with their newborn. From the latter category those after a difficult delivery spent most time licking their calves, partly in lying position. After a difficult delivery calves started their first attempts to stand later, stood later, and when kept with their mothers, suckled later, than did other calves. The behavioural differences between calves from unaided and partly aided deliveries were small. (Abstract retrieved from CAB Abstracts by CABI’s permission)

MiR-92a-3p promotes the malignant progression of hepatocellular carcinoma by mediating the PI3K/AKT/mTOR signaling pathway

2021 ◽  
Vol 27 ◽  
Author(s):  
Libing Wang ◽  
Mingxin Cui ◽  
Fengzhi Qu ◽  
Daming Cheng ◽  
Jingkun Yu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Western Blot ◽  
Signaling Pathway ◽  
Epithelial Mesenchymal Transition ◽  
Mtor Signaling ◽  
Mtor Signaling Pathway ◽  
Mesenchymal Transition ◽  
Diagnosis And Prognosis ◽  
Potential Biomarker ◽  
Hcc Cells

Background: As one of the most common cancers in the world, hepatocellular carcinoma (HCC) usually has a poor prognosis. Many HCC patients are usually diagnosed at advanced stages. Therefore, new potential biomarkers for the diagnosis and prognosis of HCC are urgently needed. More and more studies have shown that miR-92a-3p can regulate the occurrence and development of a variety of cancers, but its clinical significance and molecular mechanism in HCC are still elusive. Here, we tried to clarify the regulatory mechanism of miR-92a-3p in HCC. Methods: In this study, we conducted qRT-PCR and revealed that miR-92a-3p was notably upregulated in HCC cells. MTT, flow cytometry, wound healing, Transwell invasion assays and western blot were conducted to uncover that overexpressed miR-92a-3p could boost the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of HCC cells while inhibiting cell apoptosis. In addition, the proteins associated with PI3K/AKT/mTOR pathway were also detected by western blot. Results: It was suggested that miR-92a-3p could activate the PI3K/AKT/mTOR signaling pathway. Conclusion: These results suggest that miR-92a-3p plays a tumor-promoting role in HCC and may be a potential biomarker for the diagnosis and prognosis of HCC.

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