scholarly journals Identification of Risk Factors for Lameness Detection with Help of Biosensors

Agriculture ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 610
Author(s):  
Ramūnas Antanaitis ◽  
Vida Juozaitienė ◽  
Gediminas Urbonavičius ◽  
Dovilė Malašauskienė ◽  
Mindaugas Televičius ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Milk Fat ◽  
Milk Protein ◽  
Standard Procedure ◽  
Clinical Signs ◽  
Experimental Period ◽  
Early Lactation ◽  
Protein Ratio ◽  
Walking Activity ◽  
Protein Milk

In this study we hypothesized that the lameness of early lactation dairy cows would have an impact on inline biomarkers, such as rumination time (RT), milk fat (%), milk protein (%), milk fat/protein ratio (F/P), milk lactose (L, %), milk electrical conductivity of all udder quarters, body weight (BW), temperature of reticulorumen content (TRR), pH of reticulorumen content (pH), and walking activity (activity). All 30 lame cows (LCs) used in this experiment had a score of 3–4, identified according to the standard procedure of Sprecher et al.. The 30 healthy cows (HC) showed a lameness score of one. RT, milk fat, MY, milk protein, F/P, L, milk electrical conductivity of all udder quarters, and BW were registered using Lely Astronaut® A3 milking robots each time the cow was being milked. The TRR, cow activity, and pH of the contents of each cow’s reticulorumen were registered using specific smaXtec boluses. The study lasted a total of 28 days. Days “−14” to “−1” denote the days of the experimental period before the onset of clinical signs of lameness (day “0”), and days “1” to “13” indicate the period after the start of treatment. We found that from the ninth day before the diagnosis of laminitis until the end of our study, LCs had higher milk electrical conductivity in all udder quarters, and higher milk fat to protein ratios. On the 3rd day before the onset of clinical signs of the disease until the day of diagnosis, the milk fat of the LC group was reduced. The activity of the LCs decreased sharply from the second day to the first day after treatment. RT in the HC group tended to decrease during the experiment. pH in LCs also increased on the day of the appearance of clinical signs.

scholarly journals Influence of Calving Ease on In-Line Milk Urea and Relationship with Other Milk Characteristics in Dairy Cows

Agriculture ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1159
Author(s):  
Mindaugas Televičius ◽  
Ramūnas Antanaitis ◽  
Vida Juozaitienė ◽  
Algimantas Paulauskas ◽  
Dovilė Malašauskienė ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Dairy Cows ◽  
Somatic Cell ◽  
Milk Fat ◽  
Milk Protein ◽  
Chemical Properties ◽  
Mean Value ◽  
Protein Ratio ◽  
Negative Effect ◽  
Milk Urea

The aim of this research was to identify the relationship of calvin ease and level of in-line milk urea (MU) and other milk components, namely milk yield (MY), electrical conductivity (EC), milk fat (MF), milk protein (MP), milk fat/protein ratio (MF/MP), and somatic cell count (SCC) in dairy cows. The cows for the research were selected following such criteria: cows were tested within the period of up to the first 30 days after calving and had had a range of lactation numbers from two to four. Each selected farm housed more than 500 dairy cows and a total of 4712 calving cases from the eight dairy farms were studied and evaluated. The 4-point scale was used for the evaluation of the calving according to the point value meanings where 1 = easy, unassisted; 2 = easy, assisted; 3 = difficult, assisted; 4 = difficult, requiring veterinary intervention. A total of 4712 calving cases were researched and scored. The chemical properties of milk in all research cows were analyzed during the early phase of lactation (from the onset of calving to 30 days past calving) every day, during each milking. Cows were classified into groups according to the level of urea in milk: Group 1 had MU ≤ 15 mg/dL (12.6% of cows), Group 2 had MU 16–30 mg/dL (62.4% of cows), and Group 3 had MU > 30 mg/dL (25.0% of cows). We found that cows with milk urea levels between 16 and 30 mg/dL had the lowest incidence of dystocia, and also the highest concentration of milk lactose (ML ≥ 4.6%), the lowest mean value of milk electrical conductivity, and the lowest value of milk SCC. Dystocia increased the risk of somatic cell growth in cow’s milk above the herd average (OR = 1.364; 95% CI = 1.184–1.571, p < 0.001), and normal urea in milk reduced this risk (OR = 0.749; 95% CI = 0.642–0.869, p = 0.05). In all groups of cows, according to the level of urea in milk, the productivity of cows without dystocia at calving was higher (2.50–5.51 kg) as well as the milk protein % (0.13–0.21%) and milk lactose % (0.07–0.19%). We concluded that, in all groups of cows, according to the level of urea in milk, the productivity and milk lactose concentration of cows without dystocia at calving was higher, and lower somatic cells count and electrical conductivity values were found in the milk compared with cows diagnosed with dystocia at calving. We can state that dystocia has a negative effect on milk urea concentration and can increase the risk of mastitis.

scholarly journals Milk fatty acid profile from grazing buffaloes fed a blend of soybean and linseed oils

2015 ◽  
Vol 67 (3) ◽  
pp. 927-934 ◽  
Author(s):  
G.A. Gagliostro ◽  
E.M. Patiño ◽  
M. Sanchez Negrette ◽  
G. Sager ◽  
L. Castelli ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Milk Fat ◽  
Nutritional Value ◽  
Milk Protein ◽  
Vaccenic Acid ◽  
Total Content ◽  
Experimental Period ◽  
Fat Content ◽  
Milk Fatty Acid ◽  
Milk Protein Content

The aim of the study was to examine the changes in milk fatty acid (FA) profile of grazing buffaloes fed either low (L, 276g/d) or high (H, 572g/d) doses of a blend (70:30, wt/wt) of soybean and linseed oils. Fourteen multiparous Mediterranean buffaloes grazing on a native pasture were fed 4 kg/day of a commercial concentrate containing no supplemental oil over a pre-experimental period of ten days. The baseline milk production and composition and milk FA profile were measured over the last three days. After this pre-experimental period the animals received the same concentrate added with either the L or H oil doses for 26 additional days. Milk yield (g/animal/day) did not differ at the start (1776 ± 522 and 1662 ± 291 for L and H, respectively, P<0.622) or at the end of the trial (4590 ± 991 and 4847 ± 447 in L and H, respectively, P<0.543). Baseline milk fat content (g/kg) averaged 77.1 (±20.5) in L and 74.3 (±9.9) in H (P<0.10) and was reduced (P<0.031) to 60.7 (±23.6) and 49.4 (±11.2) (P<0.0031) respectively after L and H with no differences between treatments (P<0.277). Baseline milk protein content (L=43.2 ± 3.4 and H= 44.3 ± 6.9g/kg) increased after oil supplementation (P<0.0001) in both L (73.2 ± 6.0g/kg) and H (68.4 ± 4.9g/kg) without differences between oil doses (P<0.123). Milk fat content of 14:0 decreased after oil supplementation only in the H treatment (5.29 to 4.03, P<0.007) whereas that of 16:0 was reduced (P<0.001) at both L (24.49 to 19.75g/100g FA) and H (25.92 to 19.17g/100g FA) doses. The reduction of total content of 12:0 to 16:0 was higher (P<0.052) in H (32.02 to 23.93g/100g FA) than L (30.17 to 25.45g/100g FA). Vaccenic acid content increased (P<0.001) from 5.70 to 13.24g/100g FA in L and from 5.25 to 16.77 in H, with higher results in the in H treatment (P<0.001). Baseline rumenic acid was sharply increased (P<0.001) in L (1.80 to 4.09g/100g FA, +127%) and H (1.60 to 4.61g/100g FA, +187%) with no differences between L and H (P<0.19). Overall, these results indicate a pronounced improvement in the nutritional value of milk fat from grazing buffaloes fed little amounts (0.276g/day) of a blend of soybean and linseed oils.

scholarly journals Revisiting the Relationships between Fat-to-Protein Ratio in Milk and Energy Balance in Dairy Cows of Different Parities, and at Different Stages of Lactation

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3256
Author(s):  
Edward H. Cabezas-Garcia ◽  
Alan W. Gordon ◽  
Finbar J. Mulligan ◽  
Conrad P. Ferris
Keyword(s):  
Energy Balance ◽  
Dairy Cows ◽  
Milk Fat ◽  
Early Lactation ◽  
Protein Ratio ◽  
Linear Relationships ◽  
Holstein Friesian ◽  
Partial Correlations ◽  
Regression Slopes ◽  
Beta Hydroxybutyrate

A statistical re-assessment of aggregated individual cow data was conducted to examine trends in fat-to-protein ratio in milk (FPR), and relationships between FPR and energy balance (EB, MJ of ME/day) in Holstein-Friesian dairy cows of different parities, and at different stages of lactation. The data were collected from 27 long-term production trials conducted between 1996 and 2016 at the Agri-Food and Biosciences Institute (AFBI) in Hillsborough, Northern Ireland. In total, 1321 lactations (1 to 20 weeks in milk; WIM), derived from 840 individual cows fed mainly grass silage-based diets, were included in the analysis. The energy balance was calculated daily and then averaged weekly for statistical analyses. Data were further split in 4 wk. intervals, namely, 1–4, 5–8, 9–12, 13–16, and 17–20 WIM, and both partial correlations and linear regressions (mixed models) established between the mean FPR and EB during these periods. Three FPR score categories (‘Low’ FPR, <1.0; ‘Normal’ FPR, 1.0–1.5; ‘High’ FPR, >1.5) were adopted and the performance and EB indicators within each category were compared. As expected, multiparous cows experienced a greater negative EB compared to primiparous cows, due to their higher milk production relative to DMI. Relatively minor differences in milk fat and protein content resulted in large differences in FPR curves. Second lactation cows displayed the lowest weekly FPR, and this trend was aligned with smaller BW losses and lower concentrations of non-esterified fatty acids (NEFA) until at least 8 WIM. Partial correlations between FPR and EB were negative, and ‘greatest’ in early lactation (1–4 WIM; r = −0.38 on average), and gradually decreased as lactation progressed across all parities (17–20 WIM; r = −0.14 on average). With increasing parity, daily EB values tended to become more negative per unit of FPR. In primiparous cows, regression slopes between FPR and EB differed between 1–4 and 5–8 WIM (−54.6 vs. −47.5 MJ of ME/day), while differences in second lactation cows tended towards significance (−57.2 vs. −64.4 MJ of ME/day). Irrespective of the lactation number, after 9–12 WIM, there was a consistent trend for the slope of the linear relationships between FPR and EB to decrease as lactation progressed, with this likely reflecting the decreasing milk nutrient demands of the growing calf. The incidence of ‘High’ FPR scores was greatest during 1–4 WIM, and decreased as lactation progressed. ‘High’ FPR scores were associated with increased energy-corrected milk (ECM) yields across all parities and stages of lactation, and with smaller BW gains and increasing concentrations (log transformed) of blood metabolites (non-esterified fatty acid, NEFA; beta-hydroxybutyrate, BHB) until 8 WIM. Results from the present study highlight the strong relationships between FPR in milk, physiological changes, and EB profiles during early lactation. However, while FPR can provide an indication of EB at a herd level, the large cow-to-cow variation indicates that FPR cannot be used as a robust indicator of EB at an individual cow level.

Milk production by dairy cows fed legume grains or barley grain with or without urea as supplements to a cereal hay based diet

1990 ◽  
Vol 30 (1) ◽  
pp. 7 ◽  
Author(s):  
SC Valentine ◽  
BD Bartsch
Keyword(s):  
Milk Production ◽  
Faba Bean ◽  
Milk Fat ◽  
Dry Matter ◽  
Milk Protein ◽  
Barley Grain ◽  
Early Lactation ◽  
Milk Protein Content ◽  
Holstein Friesian ◽  
Friesian Cows

Milk production and composition was determined in Holstein-Friesian cows fed either 3.5 or 7.0 kg dry matter (DM) daily of lupin grain, pea grain, faba bean grain or barley grain with or without 1.5% added urea, as supplements to an oaten hay based diet. All the grains were hammermilled. Daily yields of milk (L), fat (kg) and protein (kg) were significantly (P<0.05) higher for cows fed lupin (20.0, 0.81, 0.57), pea (18.9, 0.80, 0.56) and faba bean (18.9, 0.79,0.55) grains compared with those of cows fed barley grain with (17.8, 0.73, 0.51) or without (18.0, 0.71, 0.52) urea. Yields of milk (L), fat (kg) and protein (kg) and milk protein content (g/kg) were significantly (P<0.05) higher when 7.0 kg DM (19.5, 0.80, 0.57, 29.6) compared with 3.5 kg DM (18.0,0.73,0.51,29.0) of grain was fed. There were no significant differences between treatments in hay DM intake by cows fed 3.5 kg DM of grain daily. A significantly (P<0.05) higher milk fat production per unit DM intake was recorded for cows fed legume compared with barley grain. There were generally higher returns above grain cost for cows fed legume compared with barley grain and for cows fed 4 kg compared to 8 kg daily of grain. It was concluded that it was more economical to feed 3.5 kg DM of legume grain compared with 3.5 kg DM of barley grain, with or without urea, as supplements for cows offered cereal hay in early lactation. However, the economics of feeding 7.0 kg DM of legume grain compared with 7.0 kg DM of barley grain and 7.0 kg DM compared with 3.5 kg DM of grain will depend on the costs of relative changes in hay intake associated with these practices.

Effects of dietary clinoptilolite supplementation on β-hydroxybutirate serum level and milk fat to protein ratio during early lactation in Holstein-Friesian cows

2020 ◽  
Vol 292 ◽  
pp. 109766 ◽  
Author(s):  
Dražen Đuričić ◽  
Blanka Beer Ljubić ◽  
Silvijo Vince ◽  
Romana Turk ◽  
Hrvoje Valpotić ◽  
...  
Keyword(s):  
Serum Level ◽  
Milk Fat ◽  
Early Lactation ◽  
Protein Ratio ◽  
Holstein Friesian ◽  
Friesian Cows

0147 Evaluating milk fat to protein ratio and milk fat to lactose ratio as indicators for early lactation disease

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 69-70 ◽  
Author(s):  
S. Paudyal ◽  
F. P. Maunsell ◽  
C. A. Risco ◽  
A. Donovan ◽  
A. De Vries ◽  
...  
Keyword(s):  
Milk Fat ◽  
Early Lactation ◽  
Protein Ratio

scholarly journals Inline Milk Lactose Concentration as Biomarker of the Health Status and Reproductive Success in Dairy Cows

Agriculture ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 38
Author(s):  
Mindaugas Televičius ◽  
Vida Juozaitiene ◽  
Dovilė Malašauskienė ◽  
Ramunas Antanaitis ◽  
Arūnas Rutkauskas ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Health Status ◽  
Reproductive Success ◽  
Dairy Cows ◽  
Somatic Cell ◽  
Milk Fat ◽  
Metabolic Disorders ◽  
Protein Ratio ◽  
Cell Counts ◽  
Lactose Concentration

In this study, cow reticulorumen content pH and temperature together with the activity were registered using smaXtec boluses, specially designed for animal care. Body weight, rumination time, milk fat/protein ratio, milk yield, milk lactose, milk electrical conductivity, milk somatic cell count and consumption of concentrated feed were registered by Lely Astronaut® A3 milking robots. The cows in this study were assigned into two groups according to the milk lactose concentration: group 1—milk lactose < 4.70% (n = 20), group 2—milk lactose ≥ 4.70% (n = 15). The following cows were further classified according to milk fat and protein ratio: F/P < 1.2 (class 1), F/P = 1.2 (class 2) and F/P > 1.2 (class 3). According to our results, we can conclude that inline registered milk lactose concentration can be used to indicate the health status and reproductive success of fresh dairy cows. Cows with an increased lactose concentration (≥4.70%) showed more activity (54.47%) and had less risk of mastitis (determined by lower milk electrical conductivity (EC) and somatic cell counts (SCC)) and metabolic disorders, determined by milk F/P. A higher glucose concentration was also apparent in the cows with higher lactose concentration. Registered lower levels of milk lactose can be used for early identification of metabolic disorders and mastitis (set at milk SCC ≥ 100 thousand/mL). Lactose levels in cows’ milk were positively associated with their reproductive success.

scholarly journals Changes in milk fatty acid composition in relation to indicators of energy balance in Holstein cows

2012 ◽  
Vol 60 (1) ◽  
pp. 29-38 ◽  
Author(s):  
Jaromír Ducháček ◽  
Mojmír Vacek ◽  
Luděk Stádník ◽  
Jan Beran ◽  
Monika Okrouhlá
Keyword(s):  
Fatty Acids ◽  
Experimental Period ◽  
Fat Content ◽  
Milk Fatty Acid ◽  
Early Lactation ◽  
Low Carbon ◽  
Protein Ratio ◽  
A Value ◽  
Significant Difference ◽  
Over Time

The contents of protein (P), fat (F), a F:P ratio, and content of individual fatty acids (FA) and groups of FA, including ratio of SFA:UFA (saturated FA-to-unsaturated FA) and MUFA:PUFA (ratio monounsaturated FA-to-polyunsaturated FA) in milk during the first 17 weeks of lactation with respect to the development of NEB demonstrated by the change in cows’ body condition. The differences in fat content were more pronounced than those in protein content. The resulting changes of the fat-to-protein ratio over time are associated with adipose tissue mobilization due to NEB, which was also confirmed by the changes in the BCS during the experimental period. The results indicate the possibility of using both fat content and fat-to-protein ratio as indicators of NEB in early lactation cows. The highest concentrations of saturated FA (SFA) were observed for C14:0, C16:0, C18:0, and also for the SFA with low carbon numbers. The highest concentrations of unsaturated FA (UFA) were determined for C18:1, C18:2, and C16:1. The significant difference in SFA:UFA between cows with decreasing of the BCS and cows without big change in the BCS after calving were not found. The changes in MUFA:PUFA in milk during the first 5 weeks of lactation differed between the cows with low and high decrease of BCS one month after calving. In cows with a greater NEB effect was observed ratio of PUFA:MUFA in milk with a value above 6.5. The results suggest the possibility of using MUFA:PUFA ratios as suitable indicators of NEB in cows after calving.

scholarly journals Influence of Days in Milk and Parity on Milk and Blood Fatty Acid Concentrations, Blood Metabolites and Hormones in Early Lactation Holstein Cows

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2081
Author(s):  
Quynh Chau Dang Van ◽  
Emilie Knapp ◽  
Jean-Luc Hornick ◽  
Isabelle Dufrasne
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Dairy Cows ◽  
Milk Fat ◽  
Blood Cholesterol ◽  
Blood Metabolites ◽  
Early Lactation ◽  
Milk Fatty Acids ◽  
Lactation Stage ◽  
Protein Milk

The objective of this paper is to study the influence of physiological factors that affect the energy balance, such as lactation stage and parity, on milk yield and composition, milk and blood fatty acid concentrations, blood metabolites and hormones in healthy early lactation cows. Thirty-two Holstein dairy cows from five Belgian commercial farms were followed. The grass silage-based diets fed to cows fell within normal composition ranges typically offered to dairy cows on commercial dairy farms in the region. Milk and blood were sampled at each official milk recording and used for the determination of milk fat and protein, milk and blood fatty acids, blood metabolites and hormones concentrations. The considered period was 7 to 150 days in milk. As lactation progressed, concentrations of milk 18:0 and 18:1c9, as well as blood non-esterified fatty acids and β-hydroxybutyrate, decreased, and those of milk C4–C14, blood cholesterol, triglycerides, insulin and IGF-I increased, agreeing with the extensive mobilization of body reserves in early lactation. Lower concentrations of milk C4–C14 and 16:0 and concomitant higher concentrations of milk 18:0 and 18:1c9 suggest a larger body reserve mobilization in first parity cows compared with greater than or equal to second parity cows. This study confirms that early lactation stage along with parity significantly influence milk fatty acids, such as 18:1, and blood metabolites and hormones, such as NEFA and insulin.

scholarly journals Effects of rumen-protected methionine supplementation on the performance of high production dairy cows in the tropics

2020 ◽  
Author(s):  
Valdir Chiogna Junior ◽  
Fernanda Lopes ◽  
Charles George Schwab ◽  
Mateus Zucato Toledo ◽  
Edgar Alain Collao-Saenz
Keyword(s):  
Body Weight ◽  
Milk Yield ◽  
Milk Fat ◽  
Milk Protein ◽  
Body Condition Score ◽  
Block Design ◽  
Energy Levels ◽  
Basal Diet ◽  
Experimental Period ◽  
Milk Composition

AbstractIncreasing methionine availability in dairy cow diets during the first third of lactation may enhance their performance and health. The aim of this study was to determine the effect of supplementing rumen-protected methionine (RPMet) in a lactation diet containing adequate protein and energy levels. Seventy-six multiparous Holstein cows (39.1 ± 6.8 kg of milk/d and 65 ± 28 DIM) were assigned to 1 of 2 dietary treatments (38/treatment) according to a randomized complete block design with a 2-wk (covariate) and 10-wk experimental period. Treatments were a basal diet (CON; 3.77 Lys:1Met); and CON + 14 g RPMet (2.97 Lys:1 Met). Individual milk samples were taken every 2 weeks to determine milk composition. Blood was collected from 24 cows on d+30 d to measure plasma AA levels. Body weight and body condition score (BCS) were measured at the beginning and the end of the experiment. The SM diet promoted higher milk yield (41.7 vs. 40.1 kg/d; P = 0.026), energy-corrected milk yield (41.0 vs. 38.0 kg/d; P ≤ 0.01), milk protein yield (1.30 vs. 1.18 kg/d; P ≤ 0.01), and milk fat yield (1.42 vs. 1.29 kg/d; P = 0.02). Higher milk protein (3.14% vs. 2.97%; P ≤ 0.01) and casein (2.39% vs. 2.28%; P≤ 0.01) and a trend (P = 0.06) for higher milk fat % (3.41% vs. 3.21%) were also observed. Both diets resulted in similar body weight, but CON-fed cows tended (P = 0.08) to have higher BCS. Higher plasma methionine levels were determined with SM compared with CON (29.6 vs. 18.4 μM; P < 0.01), but lysine and histidine were not different. Dietary supplementation of RPM improved productive performance by increasing milk yield and milk components yields, suggesting better dietary AA utilization when Met levels are adjusted in Lys-adequate lactation diets.

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