scholarly journals Endophytic Fungi of Citrus Plants

Agriculture ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 247 ◽  
Author(s):  
Rosario Nicoletti

Besides a diffuse research activity on drug discovery and biodiversity carried out in natural contexts, more recently, investigations concerning endophytic fungi have started considering their occurrence in crops based on the major role that these microorganisms have been recognized to play in plant protection and growth promotion. Fruit growing is particularly involved in this new wave, by reason that the pluriannual crop cycle likely implies a higher impact of these symbiotic interactions. Aspects concerning occurrence and effects of endophytic fungi associated with citrus species are revised in the present paper.

Author(s):  
Rosario Nicoletti

Besides a diffuse research activity on drug discovery and biodiversity carried out in natural contexts, more recently investigations concerning endophytic fungi have started considering their occurrence in crops based on the major role that these microorganisms have been recognized to play in plant protection and growth promotion. Fruit growing is particularly involved in this new wave, by reason that the pluriannual crop cycle implies a likely higher impact of these symbiotic interactions. Aspects concerning occurrence and effects of endophytic fungi associated with citrus species are revised in the present paper.


2020 ◽  
Vol 8 (9) ◽  
pp. 1321 ◽  
Author(s):  
Rosario Nicoletti ◽  
Claudio Di Vaio ◽  
Chiara Cirillo

In addition to the general interest connected with investigations on biodiversity in natural contexts, more recently the scientific community has started considering occurrence of endophytic fungi in crops in the awareness of the fundamental role played by these microorganisms on plant growth and protection. Crops such as olive tree, whose management is more and more frequently based on the paradigm of sustainable agriculture, are particularly interested in the perspective of a possible applicative employment, considering that the multi-year crop cycle implies a likely higher impact of these symbiotic interactions. Aspects concerning occurrence and effects of endophytic fungi associated with olive tree (Olea europaea) are revised in the present paper.


Author(s):  
I.V. Grynyk ◽  
◽  
V.V. Filiov ◽  

The paper is devoted to the centenary of the L.P. Symyrenko Research Station of Pomology foundation. The authors have depicted the main stages of the institution formation and development and elucidated its principal scientific achievements. The history of the station began with the creation of the greatest in Russian Empire nursery farm by the outstanding Ukrainian pomologist Levko Platonovych Symyrenko near Mliiv (the now Cherkasy region). As early as in 1912 the cultivars of different plants numbered almost to 3 thousand here. On November 25, 1920 according to the decision of the Board of the People's Commissariat of Agriculture of the Ukrainian SSR the Mliiv Horticultural and Truck Gardening Research Station was created on the basis of the above mentioned nursery. Its first director was L.P. Symyrenko’s son Volodymyr Levkovych, who embodied the creative ideas of the great-grandfather, grandfather and father, realized the cause of several generations of the Symyrenkos. He was a real founder of the Ukrainian horticulture, namely: created and developed all the attributes of Ukrainian pomology, systems of research institutions and state nurseries and the State "Pomological Book". V.L. Symyrenko, professors L.M. Ro and V.P. Popov, research worker M.A. Grossheim and others were major organizers of the station. With their direct participation the departments of horticulture, vegetable growing, entomology, agricultural meteorology, phytopathology, technical processing of fruits and small fruits, agrochemistry ets. were founded and functioned by 1927 already. In 1958 the station was named after L.P. Symyrenko. In the April of 1989 on the initiative of its then director M.M. Artemenko and with the support of the scientific collective it was reorganized as the Mliiv L.P. Symyrenko Research Institute of Horticulture of the Lisosteppe of Ukraine, in 1992 in accordance with tho order of the Presidium of the Ukrainian Academy of Agrarian Sciences (UAAS) renamed as Mliiv L.P. Symyrenko Institute of Horticulture of UAAS and in 2006 as L.P. Symyrenko Institute of Pomology of UAAS. In 2017 it was reorganized as the L.P. Symyrenko Research Station of Pomology of the Institute of Horticulture of the National Academy of Agrarian Sciences (NAAS). Today, it is one of the leading scientific institutions of the NAAS system in the horticultural branch. The scientific and research works are carried out in conformity with the four scientific and technical programmes of NAAS: “Genetical Resources of Plants”, “Horticulture and Small-Fruit Growing”, “Plant Protection” and “Agrarian Economics”. The main direction of the institution research activity is the elaboration of the scientific basis of the preservation and spread of the genetic versatility of the fruit, small fruit, nuciferous and minor crops. At present more than 3,000 varieties of fruit, small fruit, nuciferous and ornamental crops are studied in the collectional plantations. That is the valuable genetic fund of Ukraine. Today 72 varieties bred by the Mliiv Research Station have been entered into the State Register of the Plant Cultivars Favourable for the Spread in Ukraine.


Author(s):  
Cun Yu ◽  
Ying Yao

Endophytic fungi were isolated from Phoebe bournei and their diversity and antimicrobial and plant growth-promoting activities were investigated. Of the 389 isolated endophytic fungi, 88.90% belonged to phylum Ascomycota and 11.10% to phylum Basidiomycota. The isolates were grouped into four taxonomic classes, 11 orders, 30 genera, and 45 species based on internal transcribed spacer sequencing and morphologic analysis. The host showed a strong affinity for the genera Diaporthe and Phyllosticta. The diversity of the fungi was highest in autumn, followed by spring and summer, and was lowest in winter. The fungi exhibited notable tissue specificity in P. bournei, and the species richness and diversity were highest in the root across all seasons. Five isolates showed antimicrobial activity against eight plant pathogens, and reduced the incidence of leaf spot disease in P. bournei. Additionally, 9 biocontrol isolates showed plant growth-promoting activity, with five significantly promoting P. bournei seedling growth. This is the first report on the endophytic fungi of P. bournei and their potential applicability to plant disease control and growth promotion.


Author(s):  
Martino Bolognesi

New theoretic and experimental approaches to drug discovery. Environmental, demographic and ecological reasons suggest that either novel or known viruses will continue to emerge worldwide, posing new threats to the human population. Additionally, therapeutic interventions present different outcomes; for example, vaccination campaigns in the Third World often encounter local distribution problems and reach an insufficient fraction of the population. For many viruses no vaccine is available, such that the toll death is high, particularly in tropical countries and among infants. On the other hand, resistance of bacteria to know antibiotics is increasingly a serious threat, often associated to nosocomial infections. As a result, new ideas and approaches to the discovery or design of new effective drugs are a high priority in all civilized countries, to prevent and be ready to face potential pandemics. In this context, our group at the University of Milano, in collaboration with several European and extra- European labs, has been exploring the structural and functional properties of enzymes involved in the replication of (+)stranded RNA viruses as targets for the design of antiviral drugs. The rationale behind the choice of specific target viral is the idea that these are structurally and functionally sufficiently different from their human counterparts; thus, blocking the virus enzyme with a new drug should not be reflected by adverse reactions in the human host. The discovery approach applied in our laboratory has been based on a series of specific experimental steps: i) the analysis of crystal structure of the free enzymes, through X-ray crystallography; ii) in silico (computational) preliminary screening of selected enzyme regions towards which the drug search is targeted (e.g. mostly the enzyme active sites); iii) biochemical and biophysical tests of enzyme inhibition; iv) crystal structure analyses of enzyme/inhibitor complexes; v) in cell/in vivo testing; vi) inference for drug-lead optimization. This research method proved effective in discovering low molecular weight inhibitors of two key enzymes from Yellow fever virus (and partly for Dengue virus), and for Norovirus. Specifically, we targeted Norovirus studying the long known drug Suramin (used in the therapy of ‘sleeping sickness’), which was selected through the procedure described above through our in silico docking screenings. Our crystallographic and inhibition assays allowed to highlight the inhibitor binding mode and satisfactory functional inhibitory parameters. Subsequently, in the context of an international collaboration, we could test a series of Suramin molecular fragments, in search of new active compounds endowed with suitable pharmacological parameters. The described research activity, which is based on new conceptual and multidisciplinary approaches to drug discovery, has led to the production of several small molecules that will be further developed into antiviral compounds.


2005 ◽  
Vol 11 (4) ◽  
Author(s):  
Mark J Belsey ◽  
Alex K Pavlou

As part of Datamonitor's alliance and licensing strategic analysis, the authors have completed a two year survey of the trends underlying early-stage drug discovery and development collaborations between October 2002 and September 2004, which included 524 early-stage deals. Deal analysis shows that the leading pharma and biotech companies (fully integrated players) are the principal collaboration seekers, and that target and product innovation is driving the new wave of 21st century deals. These deals cover all phases of early-stage drug development, with lead product/target identification/validation accounting for the greatest proportion of collaborations. This represents a shift away from initial-stage collaborations, which are primarily focused on technologies such as genomics, as a result of the lack of tangible results that such technologies have delivered in the past. Following the continuously increasing demand for late-stage high-value products, the aim of the money and time invested in these early-stage collaborations is to reverse the pipeline productivity crisis currently affecting the industry's leaders over the mid to long term.


Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1250
Author(s):  
Markéta Kulišová ◽  
Maria Vrublevskaya ◽  
Petra Lovecká ◽  
Blanka Vrchotová ◽  
Milena Stránská ◽  
...  

Endophytes are microorganisms that live asymptomatically inside plant tissues. They are beneficial to their host in many aspects, especially as a defense against foreign phytopathogens through the production of a variety of metabolites. These substances can serve as sources of new natural products for medicinal, agricultural, and industrial purposes. This article is focused on endophytic fungi from Vitis vinifera. The purpose of the research was their isolation and identification during the Vitis vinifera growing season. Subsequently, the isolates were tested for the production of biotechnologically interesting metabolites (siderophores, antioxidants, and antifungal compounds). In total, 24 endophytic fungi were isolated, the most represented genus was Cladosporium sp. The results of the test for antioxidant and antifungal properties, as well as siderophore production, have shown that the population of Vitis vinifera endophytic microscopic fungi could serve as a promising source of metabolites with a wide range of applications.


2011 ◽  
Vol 21 (7) ◽  
pp. 992-996 ◽  
Author(s):  
Young-Hyun You ◽  
Hyeok-Jun Yoon ◽  
Gil-Seong Lee ◽  
Ju-Ri Woo ◽  
Soon-Ok Rim ◽  
...  

2019 ◽  
Vol 6 (2) ◽  
pp. 232-242 ◽  
Author(s):  
Pooja Goswami ◽  
Sonali Yadav ◽  
Jyoti Mathur

Nanotechnology is the promising field with its wide applications in biotechnology, pharmaceutical science, drug targeting, nano-medicine and other research areas. This review highlights the positive and negative impact of nanoparticles on plants and its wide applications in agricultural sciences. Effect of NPs in terms of seed germination, growth promotion and enhancement of metabolic rate has been evaluated by several scientific researches. However, NPs also exert their negative effects such as suppression of plant growth, inhibition of chlorophyll synthesis, photosynthetic efficiency etc. Effects of NPs can be either positive or negative it depending upon the plant species and type of nanoparticles used & its concentration. Modern nano-biotechnological tools have a great potential to increase food quality, global food production, plant protection, detection of plant and animal diseases, monitoring of plant growth nano-fertilizers, nano-pesticide, nano-herbicides and nano-fungicides.


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