scholarly journals Pollen Flow of Winter Triticale (x Triticosecale Wittmack) Investigated with Transgenic Line Expressing β-Glucuronidase Gene

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 431
Author(s):  
Janusz Zimny ◽  
Sławomir Sowa ◽  
Piotr Otręba ◽  
Janusz Kozdój ◽  
Aleksandra Zimny ◽  
...  
Keyword(s):  
Transgenic Line ◽  
Field Experiments ◽  
Pollen Grains ◽  
Pollen Flow ◽  
Effect Of Temperature ◽  
Atmospheric Conditions ◽  
Gene Encoding ◽  
Winter Triticale ◽  
Transgenic Lines ◽  
Transgenic Pollen

A transgenic winter triticale line expressing the uidA gene, encoding β-glucuronidase, was used to assess the pollen flow in field experiments over two consecutive vegetation seasons in central Poland. The experimental design included two variants of mixed transgenic and non-transgenic lines. Pollen grains were collected using passive traps located at 0, 10, 30, 60 and 85 meters from the transgenic line. GM pollen grains were detected histochemically by staining with x-Gluc. A positive effect of temperature increase, as well as the strength and direction of the wind on the number and spread of pollen grains was observed. Regardless of the experiment year and variant, only few pollen grains were observed at a distance of 85 m. In the first year of the study the amount of pollen grains at 85 m was 300-fold lower than at the source and 140-fold lower in the second year. The number of transgenic pollen grains was two times lower when the field with the transgenic triticale was surrounded by a non-transgenic line, compared to an empty field. On the basis of the obtained results, we suggest 100 m as the distance for triticale pollen migration, although longer flight incidents are possible in extreme atmospheric conditions.

scholarly journals Morpho-Physiological Testing of NaCl Sensitivity of Tobacco Plants Overexpressing Choline Oxidase Gene

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1102
Author(s):  
Galina N. Raldugina ◽  
Sergey V. Evsukov ◽  
Liliya R. Bogoutdinova ◽  
Alexander A. Gulevich ◽  
Ekaterina N. Baranova
Keyword(s):  
Choline Oxidase ◽  
Culture Methods ◽  
Gene Encoding ◽  
Oxidase Gene ◽  
Bacterial Enzyme ◽  
Transgenic Lines ◽  
High Regeneration ◽  
Whole Plants ◽  
Implicit Response

In this study the transgenic lines (TLs) of tobacco (Nicotianatabacum L.), which overexpress the heterologous gene encoding the bacterial enzyme choline oxidase were evaluated. The goal of our work is to study the effect of choline oxidase gene expression on the sensitivity of plant tissues to the action of NaCl. The regenerative capacity, rhizogenesis, the amount of photosynthetic pigments and osmotically active compounds (proline and glycine betaine) were assessed by in vitro cell culture methods using biochemical and morphological parameters. Transgenic lines with confirmed expression were characterized by high regeneration capacity from callus in the presence of 200 mmol NaCl, partial retention of viability at 400 mmol NaCl. These data correlated with the implicit response of regenerants and whole plants to the harmful effects of salinity. They turned out to be less sensitive to the presence of 200 mmol NaCl in the cultivation medium, in contrast to the WT plants.

scholarly journals Breeding of highly productive varieties of winter triticale resistant to pre-harvest germination of grain in the ear

2021 ◽  
Vol 36 ◽  
pp. 01022
Author(s):  
Valentina Rubets ◽  
Irina Voronchikhina ◽  
Vladimir Pylnev ◽  
Viktor Voronchikhin
Keyword(s):  
Central Region ◽  
High Resistance ◽  
Laboratory Tests ◽  
Field Experiments ◽  
Hexaploid Triticale ◽  
Winter Triticale ◽  
Russian State ◽  
Standard Level ◽  
New Varieties ◽  
Variety Testing

Creation of new varieties of winter hexaploid triticale for the central part of Russia is characterized by increased resistance of grain to germination on the root. The phenomenon of germination on the root is one of the limiting traits that limit the spread of triticale culture in areas of excessive moisture, which is the Central Region of the Non-Chernozem zone. The work was carried out at the Russian State Agrarian University – Moscow Agricultural Academy named after K.A. Timiryazev in 2018-2019. Field experiments were conducted in the fields of the Field Experimental and Breeding Stations. Laboratory tests were carried out at the Department of Genetics, Breeding and Seed Production. As a result of a comprehensive assessment of breeding varieties in the preliminary variety testing, the most valuable sample of winter triticale 228h (Castus x Don) was isolated, which formed a yield at the standard level, and during 3 years of study showed relatively high resistance to pre-harvest germination of grain in the ear. This sample is currently being intensively propagated for transfer to the State Variety Testing in 2020.

scholarly journals Modulation of Auxin Levels in Pollen Grains Affects Stamen Development and Anther Dehiscence in Arabidopsis

2018 ◽  
Vol 19 (9) ◽  
pp. 2480 ◽  
Author(s):  
Hernán Salinas-Grenet ◽  
Ariel Herrera-Vásquez ◽  
Samuel Parra ◽  
Allan Cortez ◽  
Lilian Gutiérrez ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Apical Meristem ◽  
Seed Set ◽  
Transgenic Arabidopsis ◽  
Pollen Grains ◽  
Anther Development ◽  
Anther Dehiscence ◽  
Transgenic Lines ◽  
Indole 3 Acetic Acid ◽  
Auxin Accumulation

Auxin regulates diverse aspects of flower development in plants, such as differentiation of the apical meristem, elongation of the stamen, and maturation of anthers and pollen. It is known that auxin accumulates in pollen, but little information regarding the biological relevance of auxin in this tissue at different times of development is available. In this work, we manipulated the amount of free auxin specifically in developing pollen, using transgenic Arabidopsis lines that express the bacterial indole-3-acetic acid-lysine synthetase (iaaL) gene driven by a collection of pollen-specific promoters. The iaaL gene codes for an indole-3-acetic acid-lysine synthetase that catalyzes the conversion of free auxin into inactive indole-3-acetyl-l-lysine. The transgenic lines showed several abnormalities, including the absence of short stamina, a diminished seed set, aberrant pollen tubes, and perturbations in the synchronization of anther dehiscence and stamina development. This article describes the importance of auxin accumulation in pollen and its role in stamina and anther development.

scholarly journals Development of Papaya Breeding Lines with Transgenic Resistance to Papaya ringspot virus

Plant Disease ◽  
2004 ◽  
Vol 88 (4) ◽  
pp. 352-358 ◽  
Author(s):  
Michael J. Davis ◽  
Zhentu Ying
Keyword(s):  
Transgenic Line ◽  
Carica Papaya ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Transgenic Resistance ◽  
Breeding Lines ◽  
Antisense Orientation ◽  
Resistant Lines ◽  
Sense Orientation ◽  
Transgenic Lines

Papaya (Carica papaya) was transformed via Agrobacterium-mediated transformation with four constructs containing either the unmodified or modified coat protein (CP) gene of Florida isolate H1K of Papaya ringspot virus (PRSV). The CP genes were in the sense orientation (S-CP), antisense orientation (AS-CP), sense orientation with a frame-shift mutation (FS-CP), or sense orientation mutated with three-in-frame stop codons (SC-CP). In all, 256 putative transgenic lines with the CP constructs were inoculated mechanically with PRSV H1K. None of the lines was immune to PRSV; however, highly resistant lines were found in each CP transgene group. For breeding purposes, 21 PRSV-resistant lines representing the four transgene constructs were selected and crossed with six papaya genotypes. The lines from the FS-CP and SC-CP transgene groups were highly fertile, but those from the S-CP and AS-CP transgene groups were practically infertile. Plants derived from 54 crosses and representing 17 transgenic lines were planted in the field. After 1 year in the field, 293 of the 1,258 the plants (23.3%) became naturally infected with PRSV; whereas, 29 of 30 of the nontransgenic control plants (96.7%) became infected. The incidence of PRSV infection varied in the R1 progeny depending on both the transgenic line and the nontransgenic parent.

The tillering pattern in barley varieties: II. The effect of temperature, light intensity and daylength on the frequency of occurrence of the coleoptile node and second tillers in barley

1969 ◽  
Vol 72 (3) ◽  
pp. 423-435 ◽  
Author(s):  
R. Q. Cannell
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
Light Intensity ◽  
Field Experiments ◽  
Tiller Number ◽  
Effect Of Temperature ◽  
Controlled Environment ◽  
Frequency Of Occurrence ◽  
Low Light ◽  
Late Sowing

SUMMARYControlled-environment experiments showed that development of the coleoptile node tiller (T1) was suppressed much more than that of the tiller appearing in the axil of the first true leaf (T2) by high temperature (24/15 °C; 19/10 °C; 10/6 °C), by reduced photoperiod (16 h; 12·5 h) or by low light intensity (1100 ft-c; 1000 ft-c), but minimally in the newest variety, Deba Abed. Unlike previous field experiments, the T1 tiller appeared on more Spratt Archer than Maris Badger plants. Maris Badger plants produced more T1 tillers in a high-low temperature regime (19/10 °C; 10/6 °C) than in continuous low temperature (10/6 °C). In a field experiment T1 tiller number (and yield), but not the number of other major shoots, were severely reduced by late sowing of Spratt Archer, progressively reduced in Maris Badger, but minimally in Deba Abed. This seemed to be associated with higher temperatures at later sowings.

scholarly journals Simian virus 40 (SV40)-transgenic mice that develop tumors are specifically tolerant to SV40 T antigen.

1987 ◽  
Vol 165 (2) ◽  
pp. 417-427 ◽  
Author(s):  
S J Faas ◽  
S Pan ◽  
C A Pinkert ◽  
R L Brinster ◽  
B B Knowles
Keyword(s):  
Immune Tolerance ◽  
Transgenic Line ◽  
Simian Virus 40 ◽  
Humoral Response ◽  
Simian Virus ◽  
T Antigen ◽  
T Cell Response ◽  
Cytotoxic T Cell ◽  
Sv40 T Antigen ◽  
Transgenic Lines

The ability to mount an immune response to simian virus 40 (SV40) T antigen was evaluated using mice from two distinct SV40 transgenic lines derived from injection of the same gene construct. Our studies demonstrate functional immune tolerance to SV40 T antigen in a SV40 transgenic line that consistently develops tumors of the choroid plexus by 7 mo of age. Antibodies to SV40 T antigen are undetectable in the serum of these animals; furthermore, mice from this line are unable to generate SV40-specific CTL after primary or secondary immunization with the virus, although they mount a normal CTL response to vaccinia virus when appropriately immunized. In contrast, we find that mice from a second transgenic line of low tumor incidence can mount a humoral response to SV40 T antigen, and upon immunization they generally respond with a vigorous cytotoxic T cell response to SV40 T antigen. These data suggest that specific immune tolerance to the product of an integrated viral oncogene may be induced, and is likely a reflection of the time in development at which the gene product first appears. Immune tolerance or responsiveness to the endogenous oncogene product may in turn play a role in the tumorigenic potential of such genes.

scholarly journals Temperature Response of Chickpea Cultivars to Races of Fusarium oxysporum f. sp. ciceris, Causal Agent of Fusarium Wilt

Plant Disease ◽  
2006 ◽  
Vol 90 (3) ◽  
pp. 365-374 ◽  
Author(s):  
Blanca B. Landa ◽  
Juan A. Navas-Cortés ◽  
María del Mar Jiménez-Gasco ◽  
Jaacov Katan ◽  
Baruch Retig ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Field Experiments ◽  
Temperature Response ◽  
Sowing Date ◽  
Effect Of Temperature ◽  
Sowing Dates ◽  
High Level ◽  
Moderately Resistant ◽  
Chickpea Cultivars

Use of resistant cultivars and adjustment of sowing dates are important measures for management of Fusarium wilt in chickpeas (Cicer arietinum). In this study, we examined the effect of temperature on resistance of chickpea cultivars to Fusarium wilt caused by various races of Fusarium oxysporum f. sp. ciceris. Greenhouse experiments indicated that the chickpea cultivar Ayala was moderately resistant to F. oxysporum f. sp. ciceris when inoculated plants were maintained at a day/night temperature regime of 24/21°C but was highly susceptible to the pathogen at 27/25°C. Field experiments in Israel over three consecutive years indicated that the high level of resistance of Ayala to Fusarium wilt when sown in mid- to late January differed from a moderately susceptible reaction under warmer temperatures when sowing was delayed to late February or early March. Experiments in growth chambers showed that a temperature increase of 3°C from 24 to 27°C was sufficient for the resistance reaction of cultivars Ayala and PV-1 to race 1A of the pathogen to shift from moderately or highly resistant at constant 24°C to highly susceptible at 27°C. A similar but less pronounced effect was found when Ayala plants were inoculated with F. oxysporum f. sp. ciceris race 6. Conversely, the reaction of cultivar JG-62 to races 1A and 6 was not influenced by temperature, but less disease developed on JG-62 plants inoculated with a variant of race 5 of F. oxysporum f. sp. ciceris at 27°C compared with plants inoculated at 24°C. These results indicate the importance of appropriate adjustment of temperature in tests for characterizing the resistance reactions of chickpea cultivars to the pathogen, as well as when determining the races of isolates of F. oxysporum f. sp. ciceris. Results from this study may influence choice of sowing date and use of chickpea cultivars for management of Fusarium wilt of chickpea.

scholarly journals Risk Assessment Methods for the Ringspot Pathogen Mycosphaerella brassicicola in Vegetable Brassica Crops

Plant Disease ◽  
2010 ◽  
Vol 94 (7) ◽  
pp. 851-859 ◽  
Author(s):  
Alison J. Wakeham ◽  
Roy Kennedy
Keyword(s):  
Field Experiments ◽  
Effect Of Temperature ◽  
Enzyme Linked Immunosorbent Assay ◽  
Average Wind Speed ◽  
Brussels Sprouts ◽  
Air Sampler ◽  
The United Kingdom ◽  
Average Wind ◽  
Bait Plants ◽  
Controlled Environments

Mycosphaerella brassicicola causes ringspot on Brussels sprouts, which can result in substantial yield loss in commercial production. Brussels sprout buttons are downgraded if this pathogen occurs on them. In this study, the effect of temperature and wetness duration was investigated on infection of Brussels sprouts using controlled environments (CE). The effect of temperature and wetness duration on inoculum production and ascospore discharge was also investigated. Infection by M. brassicicola was described using a mathematical model and was compared to estimates of ascospore availability obtained via a volumetric air sampler and immunofluorescence (IF). Infection of M. brassicicola was correlated (r = 0.92) with temperature during leaf wetness periods. The relationship between temperature and time to discharge of 5 and 50% of the cumulative total number of ascospores from ringspot lesions was r = 0.99 and 0.98, respectively (P < 0.001). In field experiments, an optimal wind run (the product of the average wind speed and the period over which that average speed was measured) of 250 to 500 km day-1 was required for the dissemination of ringspot inoculum to field bait plants. Quantification of M. brassicicola inoculum in collected field aerosols was possible using a monoclonal antibody in a plate-trapped antigen enzyme-linked immunosorbent assay. Precoating of the air sampler wells with sodium azide prevented trapped spores from germinating. Ringspot inoculum could be detected and quantified in air samples from commercial crops of Brussels sprouts in the United Kingdom. Low levels of ringspot inoculum measured within crops did not lead to disease development.

Sign in / Sign up

Export Citation Format

Share Document