scholarly journals Sustainable Agronomic Valorization of Unsulfured Molasses and Defatted Soybean Meal as an Optimized Formulation of Bio-Organic Fertilizer Enriched with High Cell Density P-Solubilizing Bacteria

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 996
Author(s):  
Muhamad Aidilfitri Mohamad Roslan ◽  
Izzalan Sohedein ◽  
Puan Sheau Ling ◽  
Zulfazli M. Sobri ◽  
Ali Tan Kee Zuan ◽  
...  
Keyword(s):  
Cell Density ◽  
Soybean Meal ◽  
Large Scale ◽  
Organic Fertilizer ◽  
Viable Cell ◽  
Viable Cell Density ◽  
Phosphate Solubilizing Bacteria ◽  
Maximum Cell Density ◽  
Defatted Soybean Meal ◽  
Phosphate Solubilizing

The application of plant beneficial bioinoculants such as phosphate solubilizing bacteria is a sustainable approach to expanding crop performance in agriculture. However, bioinoculant strains, particularly non-sporulating bacteria are often exposed to detrimental conditions throughout the production process and a long period of storage. This will negatively influence their viable cell density and eventually limit its efficacy in the field. To overcome such a scenario, an optimal formulation of biofertilizer should be prioritized. In this report, a sustainable valorization of molasses and defatted soybean meal as formulation of biofertilizer enriched with Enterobacter hormaechei 40a was proposed. Through the two-level factorial design and central composite design, the optimal formulation and fermentation conditions of bio-organic fertilizer to achieve maximum cell density of strain 40a were achieved. The highest cell density of strain 40a in the optimized molasses-DSM (OMD) medium was 12.56 log CFU/mL after 24 h which was 99.7% accuracy towards the predicted value. Interestingly, the solubilized P was increased by 62.4% in the OMD medium (174.07 µg/mL P) as compared to the standard P medium (65.38 µg/mL P). The shelf life of strain 40a after 180 days of storage was improved significantly around 10 log CFU/mL, when the OMD medium was amended with 0.1% sodium alginate. The strategy described here offers opportunities for agronomic formulation and large-scale bio-organic fertilizer production in the agriculture industry.

scholarly journals Mapping the molecular basis for growth related phenotypes in industrial producer CHO cell lines using differential proteomic analysis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Laura Bryan ◽  
Michael Henry ◽  
Ronan M. Kelly ◽  
Christopher C. Frye ◽  
Matthew D. Osborne ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Density ◽  
Cell Lines ◽  
Viable Cell ◽  
Viable Cell Density ◽  
High Peak ◽  
Proteomic Profiling ◽  
Cho Cell ◽  
Extended Culture ◽  
Key Pathways

Abstract Background The ability to achieve high peak viable cell density earlier in CHO cell culture and maintain an extended cell viability throughout the production process is highly desirable to increase recombinant protein yields, reduce host cell impurities for downstream processing and reduce the cost of goods. In this study we implemented label-free LC-MS/MS proteomic profiling of IgG4 producing CHO cell lines throughout the duration of the cell culture to identify differentially expressed (DE) proteins and intracellular pathways associated with the high peak viable cell density (VCD) and extended culture VCD phenotypes. Results We identified key pathways in DNA replication, mitotic cell cycle and evasion of p53 mediated apoptosis in high peak VCD clonally derived cell lines (CDCLs). ER to Golgi vesicle mediated transport was found to be highly expressed in extended culture VCD CDCLs while networks involving endocytosis and oxidative stress response were significantly downregulated. Conclusion This investigation highlights key pathways for targeted engineering to generate desirable CHO cell phenotypes for biotherapeutic production.

scholarly journals Directional Selection of Microbial Community Reduces Propionate Accumulation in Glycerol and Glucose Anaerobic Bioconversion Under Elevated pCO2

2021 ◽  
Vol 12 ◽  
Author(s):  
Pamela Ceron-Chafla ◽  
Yu-ting Chang ◽  
Korneel Rabaey ◽  
Jules B. van Lier ◽  
Ralph E. F. Lindeboom
Keyword(s):  
Microbial Community ◽  
Cell Viability ◽  
Cell Density ◽  
Viable Cell ◽  
Viable Cell Density ◽  
Directional Selection ◽  
Adaptive Laboratory Evolution ◽  
Glycerol Conversion ◽  
Microbial Groups ◽  
Selection Of

Volatile fatty acid accumulation is a sign of digester perturbation. Previous work showed the thermodynamic limitations of hydrogen and CO2 in syntrophic propionate oxidation under elevated partial pressure of CO2 (pCO2). Here we study the effect of directional selection under increasing substrate load as a strategy to restructure the microbial community and induce cross-protection mechanisms to improve glucose and glycerol conversion performance under elevated pCO2. After an adaptive laboratory evolution (ALE) process, viable cell density increased and predominant microbial groups were modified: an increase in Methanosaeta and syntrophic propionate oxidizing bacteria (SPOB) associated with the Smithella genus was found with glycerol as the substrate. A modest increase in SPOB along with a shift in the predominance of Methanobacterium toward Methanosaeta was observed with glucose as the substrate. The evolved inoculum showed affected diversity within archaeal spp. under 5 bar initial pCO2; however, higher CH4 yield resulted from enhanced propionate conversion linked to the community shifts and biomass adaptation during the ALE process. Moreover, the evolved inoculum attained increased cell viability with glucose and a marginal decrease with glycerol as the substrate. Results showed differences in terms of carbon flux distribution using the evolved inoculum under elevated pCO2: glucose conversion resulted in a higher cell density and viability, whereas glycerol conversion led to higher propionate production whose enabled conversion reflected in increased CH4 yield. Our results highlight that limited propionate conversion at elevated pCO2 resulted from decreased cell viability and low abundance of syntrophic partners. This limitation can be mitigated by promoting alternative and more resilient SPOB and building up biomass adaptation to environmental conditions via directional selection of microbial community.

Use of the mitochondria toxicity assay for quantifying the viable cell density of microencapsulated jurkat cells

2013 ◽  
Vol 29 (4) ◽  
pp. 986-993 ◽  
Author(s):  
M. Werner ◽  
K. Biss ◽  
V. Jérôme ◽  
F. Hilbrig ◽  
R. Freitag ◽  
...  
Keyword(s):  
Cell Density ◽  
Viable Cell ◽  
Viable Cell Density ◽  
Jurkat Cells ◽  
Toxicity Assay ◽  
Mitochondria Toxicity

scholarly journals Effect of temperature and agitation rate variation on the growth of NS0 cell line in monoclonal antibody production

2019 ◽  
Author(s):  
Shamitha Shetty
Keyword(s):  
Cell Density ◽  
Cell Metabolism ◽  
Viable Cell ◽  
Viable Cell Density ◽  
Effect Of Temperature ◽  
Research Report ◽  
Rate Variation ◽  
Agitation Rate ◽  
Shake Flasks ◽  
Culture Growth

The quality and yield of the monoclonal antibodies produced in a cGMP environment is heavily influenced by the bioprocess-related parameters which impact the cell growth and metabolism of the mammalian cell cultures. This research report describes a study conducted to examine the effects of varying temperature and RPM set points on viable cell density and viability of NS0 cultures. All cultures were grown in 250 mL shake flasks (working vol. 100 mL). To separately analyze the effects of temperature and agitation rate on NS0 cell metabolism, flask stage cultures were evaluated in triplicates at two cultivation temperatures (36 °C and 38 °C) and two agitation rates (120 RPM and 160 RPM) while controls were maintained at 37 °C and 140 RPM for both the conditions using an incubator. Flasks were sampled every 24 h and analyzed for viable cell density and % viability. Additional data was collected on pH, pO2, pCO2, osmolality, glucose, lactate, glutamate and glutamine levels in the culture. It was observed that variations in temperature has the greatest effect on viable cell density and viability and varying agitation rates had minimal effect on growth of cultures. A temperature set point of 38 °C is detrimental to the culture growth. The control set points proved to be optimal for this process.

scholarly journals Population of phosphate solubilizing bacteria in the liquid organic fertilizer created from palm oil bunches and pineapple rhizome

2019 ◽  
Vol 20 (11) ◽  
Author(s):  
Dermiyati Dermiyati ◽  
Radix Suharjo ◽  
Mareli Telaumbanua ◽  
Yeyen Ilmiasari ◽  
Rully Yosita ◽  
...  
Keyword(s):  
Palm Oil ◽  
Microbial Population ◽  
Organic Fertilizer ◽  
Phosphate Solubilizing Bacteria ◽  
Anaerobic Conditions ◽  
Bacterial Colony ◽  
Facultative Anaerobic ◽  
Phosphate Solubilizing ◽  
Industrial Company ◽  
Very High

Abstract. Dermiyati, Suharjo R, Telaumbanua M, Ilmiasari Y, Yosita R, Annisa RM, Sari AW, Andayani AP, Yulianti DM. 2019. Population of phosphate solubilizing bacteria in the liquid organic fertilizer created from palm oil bunches and pineapple rhizome. Biodiversitas 20: 3315-3321. Palm oil bunches and pineapple rhizomes are abundant in Indonesia, especially in Lampung Province due to widespread agro-industrial company and they are potential to be used as sources for liquid organic fertilizer. This study was aimed to reveal microbial population within liquid organic fertilizer (LF) which was developed from palm oil bunches (PB) and pineapple rhizome (PR) and their role as phosphate solubilizing bacteria (PSB). The capability to solubilize phosphate was recorded from the clear zone area around the bacterial colony which was cultivated on Pikovskaya’s agar medium. The LF was prepared in three kinds of conditions, namely aerobic, anaerobic, and facultative an aerobic. Isolation was performed every 3 days until 24 days after incubations. The results showed that microbial population in the LF from PR was significantly higher than those developed from PB. Totally, 791 bacterial isolates were obtained from the LF; 490 isolates were achieved from PR (153 isolates from aerobic, 188 isolates from facultative anaerobic, 149 from anaerobic conditions) and 301 isolates were collected from PB (96 isolates from aerobic, 112 isolates from facultative anaerobic, 93 isolates from anaerobic conditions). In the case of isolates that were gained from PB, 166 isolates (55.15%) showed capability to degrade phosphate and 135 isolates (44.85%) did not show any capability as PSB. As for isolates obtained from PR, 269 isolates (54.90%) had capability to solubilize phosphate, meanwhile, the other 221 isolates (45.10%) did not show any capability to solubilize phosphate. Among166 isolates of PSB from PB, 60 isolates had very low capability, 55 isolates had low capability, 34 isolates had medium capability, 10 isolates had high capability and only 7 isolates had very high capability. Meanwhile, from 269 isolates of PSB obtained from PR, 135 isolates had very low capability to solubilize phosphate, 84 isolates had low capability to solubilize phosphate, 32 had medium capability to solubilize phosphate, 14 isolates had high capability to solubilize phosphate and 4 isolates produced very high capability as PSB. In total, 11 chosen isolates were selected from LF developed from both PB (7 isolates) and PR (4 isolates). Therefore, palm oil bunches and pineapple rhizomes contained PSB that can be used as LF.

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