scholarly journals Postharvest Preservation of the New Hybrid Seedless Grape, ‘BRS Isis’, Grown Under the Double-Cropping a Year System in a Subtropical Area

Agronomy ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 603 ◽  
Author(s):  
Ahmed ◽  
Ruffo Roberto ◽  
Youssef ◽  
Colombo ◽  
Shahab ◽  
...  

‘BRS Isis’ is a new hybrid seedless table grape tolerant to downy mildew with a good adaptation to the tropical and subtropical climates. Gray mold, caused by Botrytis cinerea Pers. ex Fr. is known as the most important postharvest mold in table grapes, causing extensive losses worldwide. As the postharvest behavior of ‘BRS Isis’ is still unknown, the objective of this work was to evaluate the postharvest preservation and B. cinerea mold control of this new grape cultivar, grown under the double-cropping a year system. Grape bunches were purchased from a field of ‘BRS Isis’ seedless table grapes trained on overhead trellises located at Marialva, state of Parana (South Brazil). Grapes were subjected to the following treatments in a cold room at 1 ± 1°C: (i) Control; (ii) SO2-generating pad; (iii) control with bunches inoculated with the pathogen suspension; (iv) SO2-generating pad with bunches inoculated with the pathogen suspension. The completely randomized experimental design was used with four treatments, each including five replicates. The incidence of gray mold and other physicochemical variables, including bunch mass loss, shattered berries, skin color index, soluble solids (SS), titratable acidity (TA), and SS/TA ratio of grapes, were evaluated at 50 days after the beginning of cold storage and at seven days at room temperature (22 ± 2°C). The ‘BRS Isis’ seedless grape, packaged with SO2-generating pads and plastic liners, has a high potential to be preserved for long periods under cold storage, at least for 50 days, keeping very low natural incidence of gray mold, mass loss, and shattered berries.

Horticulturae ◽  
2018 ◽  
Vol 4 (3) ◽  
pp. 18 ◽  
Author(s):  
Ronan Colombo ◽  
Deived Carvalho ◽  
Maria Cruz ◽  
Ciro Sumida ◽  
Saeed Ahmed ◽  
...  

‘BRS Isis’ is a novel hybrid seedless table grape with large reddish bunches. This cultivar is cultivated in tropical and subtropical areas and has potential to be exported overseas; however, under these growth conditions, grapes can be severely damaged by gray mold (Botrytis cinerea), the main postharvest disease of table grapes. Thus, this study aimed to evaluate different pre- and postharvest strategies to extend the storage period of ‘BRS Isis’ seedless table grapes. The treatments consisted of grapes packed into carton boxes under cold storage (1 °C and high relative humidity), pre- and/or postharvest treatment with potassium bicarbonate or a biological control agent (Bacillus subtilis), all with Botrytis inoculation. Additionally, two controls, with and without Botrytis inoculation, were also evaluated. The experimental design was completely randomized with eight treatments and three replications, and each plot consisted of four bunches. After a 50-day cold storage period, the carton boxes were kept for five days at room temperature (22 °C). Gray mold incidence and water loss, as well as soluble solids (SS), pH, titratable acidity (TA), SS/TA ratio, and color attributes were evaluated during both periods. Cold storage effectively reduced gray mold in ‘BRS Isis’ seedless grape for a period of 50 days, even when grapes were inoculated; however, no difference among treatments was observed. Higher water loss was observed in both non-treated control treatments.


Horticulturae ◽  
2018 ◽  
Vol 4 (4) ◽  
pp. 29 ◽  
Author(s):  
Saeed Ahmed ◽  
Sergio Roberto ◽  
Allan Domingues ◽  
Muhammad Shahab ◽  
Osmar Junior ◽  
...  

‘Italia’ grape is one of the most important table grape cultivars grown worldwide. Gray mold, caused by Botrytis cinerea Pers. Fr., is one of the most important causes of postharvest decay of table grapes, and the control of this disease is very difficult because postharvest treatments with synthetic fungicides are not allowed in many countries. The objective of this study was to compare different types of pads releasing different doses of SO2 during cold storage to control gray mold in ‘Italia’ table grapes grown under subtropical conditions. Grape bunches were harvested from a commercial field trained on an overhead trellis located at Cambira, state of Parana (PR), South Brazil. The grapes were packed into carton boxes (capacity, 4.5 kg) and subjected to the following SO2 pad treatments (Uvasys®, Cape Town, South Africa) under cold storage (1.0 ± 1 °C) for 50 days: (i) Control; (ii) SO2 slow release pad; (iii) SO2 dual release pad; (iv) SO2 dual release–fast reduced pad; (v) SO2 slow release pad with grapes inoculated with B. cinerea suspension; (vi) SO2 dual release pad with grapes inoculated with B. cinerea suspension; and (vii) SO2 dual release-fast reduced pad with grapes inoculated with B. cinerea suspension. After cold storage, the grape boxes were maintained for 7 days at room temperature (25 °C). The incidence of gray mold on the grapes, firmness, shattered berries, stem browning, as well as other physicochemical variables, such as bunch mass, bunch mass loss, skin color, soluble solids (SS), titratable acidity (TA) and SS/TA were evaluated. Both SO2 dual release pads were highly efficient in preventing the incidence of gray mold in ‘Italia’ grapes packed in clamshells during the 50-day period of cold storage and at room temperature, even with Botrytis-inoculated berries. The SO2 slow release pad showed lower efficiency, but was higher than the control. The SO2 dual release pad treatments provided the best results with respect to stem browning scores (fresh and green stems) during cold storage, and no differences were observed among the treatments with respect to the other physicochemical evaluations.


Horticulturae ◽  
2018 ◽  
Vol 4 (3) ◽  
pp. 17 ◽  
Author(s):  
Allan Domingues ◽  
Sergio Roberto ◽  
Saeed Ahmed ◽  
Muhammad Shahab ◽  
Osmar José Chaves Junior ◽  
...  

‘BRS Vitoria’ (Vitis spp.) is a novel hybrid seedless table grape recommended for cultivation in tropical and subtropical areas, especially for overseas export. The main postharvest disease of this cultivar is botrytis or gray mold (Botrytis cinerea), which occurs even under low temperatures in cold chambers. Sulfur dioxide (SO2) release pads have been used to control this disease under cold storage, but some grape cultivars are sensitive to certain levels of this compound. The objective of this work was to evaluate different types of SO2 generator pads in order to prevent the incidence of gray mold of ‘BRS Vitoria’ seedless grape, as well to avoid other grape injuries during cold storage. Grape bunches were harvested when fully ripened (16°Brix) from a commercial field trained on overhead trellis and located at Marialva, state of Parana (PR) (South Brazil). Grapes were packed into carton boxes and subjected to the following SO2 pad treatments (Uvasys®, Cape Town, South Africa) in a cold chamber (2 °C): (a) control; (b) SO2 slow release pad; (c) SO2 dual release pad; (d) SO2 dual release–fast reduced pad; (e) SO2 slow release pad with grapes inoculated with B. cinerea; (f) SO2 dual release pad with grapes inoculated with B. cinerea; and (g) SO2 dual release–fast reduced pad with grapes inoculated with B. cinerea. After a 50-day cold chamber period, the grape boxes were kept for 7 days at room temperature at 25 °C. A randomized design was used with seven treatments and four replications, with five bunches per plot. The incidence of gray mold on grapes was evaluated after the 50-day cold storage and after the 7-days-at-room-temperature periods, as well other grape physicochemical variables, such as shattered berries, stem browning, bunch mass, bunch mass loss, skin color, soluble solids (SS), titratable acidity (TA), and SS/TA. The dual release pads were more efficient in preventing the incidence of gray mold and mass loss in ‘BRS Vitoria’ seedless grapes than the slow release pads in both storage periods. The incidence of shattered berries was lower when any type SO2 pad was used during cold storage, and no effects were observed on stem browning, firmness, or berry skin color of ‘BRS Vitoria’ grapes.


2019 ◽  
Vol 5 (1) ◽  
pp. 52 ◽  
Author(s):  
Khamis Youssef ◽  
Sergio Roberto ◽  
Ronan Colombo ◽  
Marcelo Canteri ◽  
Kamel Elsalam

The objective of this work was to investigate the effect of the resistance inducer Acibenzolar-S-methyl (ASM), against Botrytis mold on table grapes in vitro and in vivo. To assess the effect of ASM on mycelial growth Botrytis cinerea, different concentrations (0.125, 0.25, 0.5, 1.0, 2.0 and 3% w:v) were tested. Treatments were set up in triplicate, in a completely randomized experimental design, and replicated twice. Results were expressed in minimum inhibitory concentrations and effective dose per 50% response of mycelial growth. Healthy table grapes (cvs. Italia and Benitaka) were harvested at full ripe to evaluate the ASM 1% effect against gray mold under artificial conditions. Bunches were split into two groups in order to perform two types of experiments (spray or immersion). For both trials, treated bunches were arranged in carton boxes and stored at 2±1 °C, for one month, followed by one week of shelf-life at 22±2 °C. In order to evaluate the ASM effect against gray mold under field conditions, ASM 1% was sprayed on both cultivars one week before harvest. Grapes treated with iprodione 0.2% with three applications during the season were included as a standard chemical control. Bunches were harvested at full ripe, arranged in carton boxes and submitted to a cold storage process as described previously. Results for the in vitro experiments showed that the minimum inhibitory concentration of ASM was achieved by 3% and the ED50 was 0.04%. Under artificial conditions, for both cultivars, the efficacy of ASM was higher when the grapes were immersed than sprayed. A significant difference was observed for ASM as compared with control. Regarding the effect of ASM against gray mold in the field, the incidence of gray mold was recorded for both cultivars. For ‘Italia’ and ‘Benitaka’ grapes, ASM, iprodione and sulfur dioxide pad reduced the incidence of gray mold by 85, 79 and 77%, and by 80.5, 73 and 82%, respectively. As for the physico-chemical berry properties, none of the treatments were significantly different from the control for total soluble solids, titratable acidity and color index. A single ASM treatment applied one week before harvest is effective for controlling gray mold in ‘Italia’ and ‘Benitaka’ table grapes.


2020 ◽  
Vol 41 (6supl2) ◽  
pp. 3457-3465
Author(s):  
Ronan Carlos Colombo ◽  
◽  
Deived Uilian de Carvalho ◽  
Maria Aparecida da Cruz ◽  
Ciro Hideki Sumida ◽  
...  

The demand for high-quality nutritional products has increased fruit consumption, as grapes, for this reason postharvest techniques are required to prevent losses, to preserve quality, to extend shelf life, and to attend to consumer needs. In this way, the objective of this study was to evaluate strategies to control gray mold caused by Botrytis cinerea in ‘BRS Nubia’ grapes during cold storage and shelf life periods. Grape bunches were harvested from a commercial vineyard in Marialva, Parana, Brazil. Grapes were subjected to the following treatments: cold storage at 2 ºC (control), cold storage at 2 ºC with SO2-generating pads, cold storage at 2 ºC and inoculated with B. cinerea suspension, and cold storage at 2 ºC with SO2-generating pads and inoculated with B. cinerea suspension. The experiment was conducted in a complete randomized design with five replications per treatment using four bunches per experimental unit. A factorial arrangement (absence/presence of SO2 pads × absence/presence of Botrytis inoculation) was applied. At the end of 30 days of cold storage and 7 days of shelf life (22 ºC), gray mold incidence, shattered berries, and physicochemical parameters were evaluated. The gray mold incidence on ‘BRS Nubia’ grapes decreased when SO2-generating pads were used during cold storage. Berry weight loss was greater in the treatments without SO2-generating pads after 30 days of cold storage followed by 7 days of shelf life. Berry firmness, soluble solids content (SS), total acidity (TA), SS/TA ratio, and anthocyanins concentration were not negatively affected by SO2-generating pad treatments. However, a slight increase in the shattered berries percentage was recorded for the SO2-generating pad treatments. No significant quality loss of ‘BRS Nubia’ grape was evident after 30 days of cold storage followed by 7 days of exposure at room temperature. In this context, SO2-generating pads can be used to control the gray mold incidence on ‘BRS Nubia’ table grapes during cold storage.


Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 271 ◽  
Author(s):  
Osmar Jose Chaves Junior ◽  
Khamis Youssef ◽  
Renata Koyama ◽  
Saeed Ahmed ◽  
Allan Ricardo Dominguez ◽  
...  

The use of vented clamshells has become popular in the packaging of grapes for local and international markets. The aim of this study is to evaluate the postharvest preservation of ‘Benitaka’ table grapes individually packaged in vented clamshells using different types of SO2-generating pads and perforated plastic liners during cold storage. A completely randomized design with four replications in a two-factor arrangement with an additional treatment [(4 × 3) + 1] was used. The trials were carried out under two situations: Artificial or natural infections with Botrytis cinerea, which is the causal agent of gray mold on table grapes. The incidence of gray mold, shattered berries, and stem browning were evaluated at 30 and 45 days of cold storage at 1 ± 1 °C and 3 days of shelf-life at 22 ± 1 °C after the period of cold storage. Mass loss and berry firmness were also examined at the end of the cold storage period. The use of dual-release SO2-generating pads containing 5 or 8 g of a.i. and slow-release pads with 7 g of a.i. was effective in controlling the incidence of gray mold in grapes packaged in vented clamshells and kept under cold storage for up to 45 days. Under these storage conditions, perforated plastic liners with 0.3% ventilation area or micro-perforated liners with 1.0% ventilation area reduced the percentage of mass loss and shattered berries.


2003 ◽  
Vol 38 (10) ◽  
pp. 1139-1148 ◽  
Author(s):  
Luiz Carlos Argenta ◽  
Juliana Golin Krammes ◽  
Clarice Aparecida Megguer ◽  
Cassandro Vidal Talamini Amarante ◽  
James Mattheis

The inhibition of ethylene action by 1-methylcyclopropene (1-MCP) extends shelf and storage life of many climacteric fruits. However, 1-MCP appears to have limited effects on stone fruit depending on specie and cultivar. The effects of 1-MCP on ripening and quality of 'Laetitia' plums were determined during ripening at 23ºC following harvest and cold storage. Japanese plums (Prunus salicina, cv. Laetitia) were harvested at mature pre-climacteric stage, cooled to 2ºC within 36 hours of harvest and then treated with 0, 0.05, 0.10, 0.50 or 1.00 muL L-1 of 1-MCP at 1°C for 24 hours. Following treatment, fruits were either held at 23ºC for 16 days or stored at 1ºC for 50 days. Fruits were removed from cold storage at 10-day intervals and allowed to ripe at 23°C for five days. A delay of climacteric respiration and ethylene production by 1-MCP treatment during ripening following harvest and cold storage was associated to a slow rate of fruit softening. 1-MCP treatment also delayed the loss of titratable acidity and changes of flesh and skin color, whereas it had little or no effect on soluble solids content. 1-MCP effects were concentration- and storage duration-dependent and, generally, a saturation fruit response to 1-MCP occurred between 0.5 and 1.0 muL L-1. During ripening, 1-MCP treated fruits attained quality similar to that of controls. Results indicated that 1-MCP treatment may extend shelf life (23ºC) and storage life (1ºC) of 'Laetitia' plums by approximately six and 20 days, respectively.


2017 ◽  
Vol 39 (5) ◽  
Author(s):  
MARIUCCIA SCHLICHTING DE MARTIN ◽  
CRISTIANO ANDRÉ STEFFENS ◽  
CASSANDRO VIDAL TALAMINI DO AMARANTE ◽  
AURI BRACKMANN ◽  
MARÍLIA FARIAS RODRIGUES ◽  
...  

ABSTRACT The storage of ‘Rocha’ pears under controlled atmosphere (CA) preserves fruit quality for long periods. However, inadequate CA conditions might impair ripening and lead to flesh browning. This research was carried out to assess the effects of CA with ultra-low (ULO), and low O2 (LO) associated with different CO2 levels on ripening and occurrence of flesh browning in ‘Rocha’ pears. Treatments evaluated were: pO2 = 0.5 kPa (ULO) and pCO2 < 0.03 kPa; pO2 = 1.0 kPa (LO) and pCO2 < 0.03 kPa; pO2 = 1.0 kPa and pCO2 = 1.0 kPa; pO2 = 1.0 kPa and pCO2 = 2.0 kPa; and pO2 = 1.0 kPa and pCO2 = 3.0 kPa. A completely randomized experimental design was used, with four replicates. Fruits were harvested in Vacaria, RS, and stored under five CA conditions during 270 days (-0.5±0.1 ºC and relative humidity of 96±2%). Fruits were assessed after CA storage for respiratory rate, ethylene production, skin color, flesh firmness, texture, titratable acidity (TA), soluble solids content (SSC), sensory attributes, flesh browning incidence and severity and flesh color. Fruits stored under LO with pCO2 < 0.03 kPa had higher flesh firmness and more yellow skin color than fruits stored under other CA conditions. Sensory attributes, SS and TA of fruits were not affected by CA conditions. Fruit stored under LO with pCO2 = 3.0 kPa had the highest incidence (48%) and severity of flesh browning. The pO2 = 0.5 kPa and pCO2 < 0.03 kPa and pO2 = 1.0 kPa and pCO2 = 1.0 kPa conditions are the most suitable for the CA storage of ‘Rocha’ pears.


Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 792
Author(s):  
Antonino Pisciotta ◽  
Diego Planeta ◽  
Simone Giacosa ◽  
Maria Alessandra Paissoni ◽  
Rosario Di Lorenzo ◽  
...  

The aim of this study was to evaluate the influence of paper bagging of grape bunches on the morphological, mechanical, and chemical characteristics of berries of three table grapes varieties as an environmentally-friendly technique for protecting clusters from biotic and abiotic agents. Clusters of Italia, Autumn Royal, and Regal Seedless grape cultivars were bagged and compared to a not-bagged control. Air temperature inside and outside the bags was monitored. Bunch weight and length, number of berries per bunch, berry longitudinal and transversal diameter, berry mass, number of seeds per berry (normal in size and aborted), soluble solid content, titratable acidity, and skin color by CIEL*a*b* parameters were determined on four points of each berry. Berries were evaluated using texture analysis, and the main texture profile analysis parameters were compared. The air temperatures around not-bagged clusters were slightly higher than inside the bags. In all the cultivars under evaluation, bagged bunches were heavier compared with not-bagged ones. In Autumn Royal and Regal Seedless, these differences were mainly owing to the higher number of berries and higher berry weight of the bagged bunches. Regarding mechanical properties, in seedless varieties, the bagging treatment increased berry hardness (related to the berry firmness) and decreased berry cohesiveness and resilience, whereas an opposite behavior was found in cv. Italia. Berry skin break force was lower in the bagging treatment in all the analyzed varieties, indicating a softer and easier-to-chew berry skin. The findings demonstrate that the bagging technique affected the three variety parameters to different extents. The main differences were found in the seedless varieties in terms of berry size and bunch characteristics. For all varieties, bagged bunches achieved the quality level required by the market, confirming the suitability of this technique. However, the bag industry is proposing many different bag types (differing in material, shape, color, and closing system); therefore, further studies are needed to obtain more complete and exhaustive technical information.


2015 ◽  
Vol 45 (3) ◽  
pp. 386-391 ◽  
Author(s):  
Josuel Alfredo Vilela Pinto ◽  
Márcio Renan Weber Schorr ◽  
Fabio Rodrigo Thewes ◽  
Deiverson Luiz Ceconi ◽  
Vanderlei Both ◽  
...  

This study aimed to evaluate the effect of different levels of relative humidity (RH) on the maintenance of Postharvest quality of 'Niagara Rosada' table grapes after cold storage at 0.5°C for 21 days. A completely randomized design was used, with five replicates of 18 clusters per treatment. The selected levels of relative humidity were 85, 90, 95 and 100%, obtained by means of electronic humidity controllers. The fruits were evaluated in relation to rot incidence, berry cracking, browning rate, respiratory rate, titratable acidity, soluble solids, resistance to abscission, berry drop and weight loss. The evaluations were assessed at 20 days of storage and after two and four days of shelf-life (20°C; 85% RH). The results showed that as higher as relative humidity, higher is rot incidence and that the variables rachis browning and weight loss were inversely related to relative humidity. Resistance to abscission at the end of storage was higher when humidity ranged between 90 and 95%, but it was not affected after transfer to shelf-life from cold storage. Relative humidity ranging between 90 and 95% offer the best conditions to maintain the Postharvest quality of 'Niagara Rosada' table grapes


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