scholarly journals Dynamic of Vascular Streak Dieback Disease Incidence on Susceptible Cacao Treated with Composted Plant Residues and Trichoderma asperellum in Field

Agronomy ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 650 ◽  
Author(s):  
Ade Rosmana ◽  
Muhammad Taufik ◽  
Asman Asman ◽  
Nurul Jihad Jayanti ◽  
Andi Akbar Hakkar
Keyword(s):  
Soil Amendment ◽  
Large Scale ◽  
Disease Incidence ◽  
Organic Fertilizer ◽  
Time Span ◽  
Plant Residues ◽  
First Year ◽  
Trichoderma Asperellum ◽  
Aerial Parts ◽  
Leaf Chlorosis

Trichoderma asperellum, composted plant residues, and its combination can control vascular-streak dieback (VSD) disease caused by fungus Ceratobasidium theobromae in laboratory conditions. In this trial, we evaluated these treatments in two years through the application of foliar spraying and stem infusion for T. asperellum alone, through soil amendment for compost alone, and T. asperellum plus this organic fertilizer in the field on susceptible cacao clone. The disease is characterized by full-leaf chlorosis and necrosis that can develop rapidly to the entire branch, with around 70% incidence in seven months, and we detected the pathogen in branches showing light symptoms. All treatments except for T. asperellum plus composted plant residues three and seven months post application did not have any impact on the reduction of VSD incidence in the first year. In the second year, we observed a significant reduction of the disease by both T. asperellum in combination with compost and compost alone in a time span of three to seven months, and with T. asperellum spraying and infusion in a time span of five to seven months. By comparing to the control, the efficacy of these treatments in controlling the VSD disease seven months post-first application in the last year was 44.4%, 23.5%, 23.1%, and 15.1%, respectively. Detection of trees inoculated with T. asperellum indicated that this fungus was present in root and branch tissues except for treatment through infusions, while in the uninoculated trees, Trichoderma was not present or was present at a very low level. These data showed that combination of T. asperellum and composted plant residues applied through soil amendment was able to control VSD disease effectively and could potentially be used at large scale to control this disease and other diseases infesting aerial parts of cacao, and to improve soil fertility.

scholarly journals Shallot Basal Bulb Rot Management through Integration of Trichoderma asperellum, Composted Plant Residues and Natural Mulch

2020 ◽  
Vol 14 (3) ◽  
pp. 1779-1788
Author(s):  
Nurmasita Ismail ◽  
Ade Rosmana ◽  
Sylvia Sjam ◽  
Ratnawati Ratnawati
Keyword(s):  
Large Scale ◽  
Fungal Colonization ◽  
Plant Residues ◽  
Trichoderma Asperellum ◽  
Organic Mulch ◽  
Allium Cepa L ◽  
Leaf Tissues ◽  
Basal Bulb ◽  
Bulb Rot ◽  
Root Tissues

Basal bulb rot (BBR) of shallot caused by Fusarium oxysporum f. sp. cepae is one of the highly deleterious diseases on shallot, Allium cepa L. var ascalonicum Backer. in Indonesia. This study aimed to assess the potency of organic mulch, composted plant residues, and endophytic Trichoderma asperellum to control this disease. Treatment with mulch alone, mulch plus compost, mulch plus T. asperellum, and combination of all the three provided the decrease of BBR incidence by 15%, 20%, 29%, and 39% and the increase of shallot productivity by 22%, 66%, 84%, and 125%, respectively. Observation of another treatment impact on the fungal occurrence at harvest time indicated that their population in soil increased by 671%, 771%, 257%, and 814% and the fungal colonization in root tissues mounted by 31%, 77%, 77%, and 74%, respectively. The introduced Trichoderma was found predominantly, especially in leaf tissues of inoculated shallot. These data showed that all the treatments were able to control BBR disease. However, the most effective was the mulch in combination with compost and T. asperellum. Therefore, large-scale disease control could take advantage of this integration.

scholarly journals Large-scale transcriptomics to dissect 2 years of the life of a fungal phytopathogen interacting with its host plant

BMC Biology ◽  
2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Elise J. Gay ◽  
Jessica L. Soyer ◽  
Nicolas Lapalu ◽  
Juliette Linglin ◽  
Isabelle Fudal ◽  
...  
Keyword(s):  
Host Plant ◽  
Large Scale ◽  
Field Experiments ◽  
Plant Residues ◽  
Plant Infection ◽  
Effector Genes ◽  
Controlled Conditions ◽  
Niche Adaptation ◽  
Depth Analysis ◽  
Fungal Genes

Abstract Background The fungus Leptosphaeria maculans has an exceptionally long and complex relationship with its host plant, Brassica napus, during which it switches between different lifestyles, including asymptomatic, biotrophic, necrotrophic, and saprotrophic stages. The fungus is also exemplary of “two-speed” genome organisms in the genome of which gene-rich and repeat-rich regions alternate. Except for a few stages of plant infection under controlled conditions, nothing is known about the genes mobilized by the fungus throughout its life cycle, which may last several years in the field. Results We performed RNA-seq on samples corresponding to all stages of the interaction of L. maculans with its host plant, either alive or dead (stem residues after harvest) in controlled conditions or in field experiments under natural inoculum pressure, over periods of time ranging from a few days to months or years. A total of 102 biological samples corresponding to 37 sets of conditions were analyzed. We show here that about 9% of the genes of this fungus are highly expressed during its interactions with its host plant. These genes are distributed into eight well-defined expression clusters, corresponding to specific infection lifestyles or to tissue-specific genes. All expression clusters are enriched in effector genes, and one cluster is specific to the saprophytic lifestyle on plant residues. One cluster, including genes known to be involved in the first phase of asymptomatic fungal growth in leaves, is re-used at each asymptomatic growth stage, regardless of the type of organ infected. The expression of the genes of this cluster is repeatedly turned on and off during infection. Whatever their expression profile, the genes of these clusters are enriched in heterochromatin regions associated with H3K9me3 or H3K27me3 repressive marks. These findings provide support for the hypothesis that part of the fungal genes involved in niche adaptation is located in heterochromatic regions of the genome, conferring an extreme plasticity of expression. Conclusion This work opens up new avenues for plant disease control, by identifying stage-specific effectors that could be used as targets for the identification of novel durable disease resistance genes, or for the in-depth analysis of chromatin remodeling during plant infection, which could be manipulated to interfere with the global expression of effector genes at crucial stages of plant infection.

scholarly journals The W Model, a Systematic Engineering for Water Source Clearance, Renewable Energy Production, and Ecological Agricuture Development – A Proposal for Sustainable Development

2017 ◽  
Vol 10 (5) ◽  
pp. 143
Author(s):  
Tongyuan Wang
Keyword(s):  
Renewable Energy ◽  
Large Scale ◽  
Organic Fertilizer ◽  
Water Source ◽  
Small Towns ◽  
Living Environment ◽  
Economic Feasibility ◽  
Organic Fertilizers ◽  
Ecological Agriculture ◽  
Agriculture Development

This article proposes a systematic engineering for sustainable economic and ecologic development. This system is deemed to be applicable in any country of the world. The system aims to realize five important objectives: water source clearance, energy saving and emission reduction, renewable energy and organic fertilizer production, and ecological agriculture development, all in large scale and at low cost. The main conception of the new system to reach these goals is the replacement of the conventional sewage treatment approach with more efficient and more ecological process – the natural fermentation of the mixture of the urban sewage and agrarian wastes, such that water body clearance, including water de-eutrophication, green algae prevention and siltation dredging will all be accomplished at virtually a zero cost. Along with this process, the system can produce a vast amount of renewable energy and organic fertilizers, consequently ecological agriculture development in large scale can be realized. As a result, this system will greatly reduce the use of chemical fertilizers thus largely reduce the consumption of fossil energy and the related polluting emissions. This system is thus fully a circular economy model through full west-reuse processes, which ultimately will enhance our life quality with healthier food and living environment. The system is flexible and adaptable to be implemented in either small towns or megacities. The implementation and operation of this system will also benefits employment growth. Lastly, in terms of economic feasibility and profitability, millions to billions of dollars of annual revenue can be generated from the running of this system in a country.

scholarly journals BIOGAS PRODUCTION FROM AMARANTH BIOMASS

2013 ◽  
Vol 10 (2) ◽  
pp. 59-62
Author(s):  
Vladimír Sitkey ◽  
Ján Gaduš ◽  
Ľubomír Kliský ◽  
Alexander Dudák
Keyword(s):  
Dry Matter ◽  
Large Scale ◽  
Biogas Production ◽  
Organic Fertilizer ◽  
Matter Content ◽  
Raw Material ◽  
Dry Matter Content ◽  
Optimum Conditions ◽  
Average Yield ◽  
Amaranthus Spp

Abstract Energy variety of amaranth (Amaranthus spp.) was grown in large-scale trials in order to verify the capability of its cultivation and use as a renewable energy source in a biogas plant. The possibility of biogas production using anaerobic co-fermentation of manure and amaranth silage was verified in the experimental horizontal fermentor of 5 m3 volume, working at mesophilic conditions of 38-40 °C. The goal of the work was also to identify the optimum conditions for growth, harvesting and preservation of amaranth biomass, to optimize biogas production process, and to test the residual slurry from digestion process as a high quality organic fertilizer. The average yield of green amaranth biomass was 51.66 t.ha-1 with dry matter content of 37%. Based on the reached results it can be concluded that amaranth silage, solely or together with another organic materials of agricultural origin, is a suitable raw material for biogas production.

scholarly journals Cambridge Servicemen’s Memorial (Breuer, 1945): espacio público para el recuerdo

2019 ◽  
Vol 6 (2) ◽  
pp. 27
Author(s):  
Salvador José Sanchís Gisbert ◽  
Pedro Ponce Gregorio ◽  
Ignacio Peris Blat
Keyword(s):  
Public Space ◽  
Large Scale ◽  
Residential Buildings ◽  
Small Scale ◽  
First Year ◽  
Residential Land ◽  
The Public ◽  
International Recognition ◽  
The World ◽  
Strong Commitment

Marcel Breuer was in the first year of architectural technicians to graduate from Bauhaus School. The peculiar education he received there allowed him to explore the concept of design in its broadest sense. In his European stage we find, on the most private and small scale, unique solutions for furniture. In his first American stage we see a strong commitment with solutions related to the residential land and, when he earned international recognition, he developed large scale solutions for his public non-residential buildings and urban equipments in locations all over the world. It is strange to see that an architect like him did not have the opportunity to materialize any of his proposals associated with the public space. The 1945 Cambridge Servicemen’s Memorial project, also known as the Memorial War, is the most significant one he developed in his last years in Cambridge. Had it been built, it would have been a valuable example of modernity and contemporary reinterpretation of the monument in the public space.

scholarly journals Influence of green manure phytomass treatment by microbial preparations on grain productivity and quality indicators of winter wheat

2020 ◽  
Author(s):  
A.V. Prikhodko ◽  
◽  
I.A. Kameneva ◽  
A.I. Yakubovskaya ◽  
N.V. Karaeva ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Green Manure ◽  
Organic Fertilizer ◽  
Point Of View ◽  
Plant Residues ◽  
Net Income ◽  
Economic Point ◽  
Yield Increase ◽  
Stage Of Development ◽  
Biological Preparation

Treatment of the triticale phytomass with the complex biological preparation KBP-5M before incorporation into the soil contributed to the activation of microbiological processes in the 0-30 cm layer. In the variants when the plant was in the phenological stage of spike emergence, we noted an increase in the content of N-NO3 by 0.19-0.46 mg per 100 g of soil; Р2О5 – up to 2.97; K2О – up to mg/100 g. The reason for that was the maximum accumulation of organic matter in the phytomass of triticale at the aforementioned stage of development (8.25-8.52 t/ha). The use of KBP-5M had a positive effect on the development of winter wheat when green manure was the preceding crop. There is a tendency to increase the density of the stalk by 13.3 %, including productive stems – by 3.9 % and the number of grains from 10 plants – by 1.7 % compared to the use of triticale plants as an organic fertilizer without the treatment with biological preparation. Despite the trend of increasing yields from 4.32 to 4.43 t/ha, no reliable yield increase was obtained. When applying KBP-5M on triticale plant residues, there was an increase in protein content by 0.3 % and gluten – by 1.1 % compared to variants without the biological preparation. According to the indicators of 1000-grain weight and hectolitre weight, there were no significant differences between the studied variants. The use of KBP-5M has been justified from an economic point of view since 277 to 1535 rubles per ha of contingent net income was obtained and the payback ratio for the use of the biological product was 1.05–5.79.

scholarly journals Inhibitory Effect of Trichoderma asperellum Isolate against Ralstonia solanacearum Causing Brinjal Wilt

2021 ◽  
Vol 24 (2) ◽  
pp. 107-120
Author(s):  
SMN Islam ◽  
SS Siddique ◽  
MZH Chowdhury ◽  
NJ Mishu
Keyword(s):  
Ralstonia Solanacearum ◽  
Disease Incidence ◽  
Its Region ◽  
Inhibitory Effect ◽  
Dual Culture ◽  
Trichoderma Asperellum ◽  
In Planta ◽  
Quantitative Test ◽  
Trichoderma Isolate

A native Trichoderma isolate was collected from the agricultural soil of Gazipur. This isolate was identified as a Trichoderma asperellum through morphology and analysis of internal transcribed spacer (ITS) region of ribosomal RNA gene sequence and reconstruction of the phylogenetic tree. The antagonistic effects of the newly identified T. asperellum isolate were assessed against brinjal bacterial wilt caused by Ralstonia solanacearum both in vitro and in planta. Both qualitative and quantitative bioassays were conducted in vitro. For qualitative tests, dual culture and antibacterial activity were carried out, and pathogen growth was observed visually. The antagonism of T. asperellum cell free culture filtrate on the growth of R. solanacearum was conducted in a quantitative test. Successful antagonism was recorded after both in vitro qualitative tests. In addition, the lowest colony forming unit was recorded in 100% of CFC (2.4±0.51 ×103 cfu/ml) in quantitative test. The T. asperellum inoculated plant showed low disease incidence (13.33%) when seedlings were challenged with R. solanacearum in planta experiment. Disease incidence was 100% for seedlings when treated with only R. solanacearum. The results showed that the isolated and identified T. asperellum isolate suppressed R. solanacearum growth in vitro and protected the seedling from wilting in planta. Therefore, this isolate could be considered as a potential isolate. Ann. Bangladesh Agric. (2020) 24(2) : 107-120

Regional Spatial-Temporal Spread of Citrus Huanglongbing Is Affected by Rain in Florida

2018 ◽  
Vol 108 (12) ◽  
pp. 1420-1428 ◽  
Author(s):  
M. M. Shimwela ◽  
T. S. Schubert ◽  
M. Albritton ◽  
S. E. Halbert ◽  
D. J. Jones ◽  
...  
Keyword(s):  
Large Scale ◽  
Disease Incidence ◽  
Kernel Density ◽  
Candidatus Liberibacter Asiaticus ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Density Analysis ◽  
Liberibacter Asiaticus ◽  
Kernel Density Analysis ◽  
Temporal Spread

Citrus huanglongbing (HLB), associated with ‘Candidatus Liberibacter asiaticus’ (Las), disseminated by Asian citrus psyllid (ACP), has devastated citrus in Florida since 2005. Data on HLB occurrence were stored in databases (2005 to 2012). Cumulative HLB-positive citrus blocks were subjected to kernel density analysis and kriging. Relative disease incidence per county was calculated by dividing HLB numbers by relative tree numbers and maximum incidence. Spatiotemporal HLB distributions were correlated with weather. Relative HLB incidence correlated positively with rainfall. The focus expansion rate was 1626 m month−1, similar to that in Brazil. Relative HLB incidence in counties with primarily large groves increased at a lower rate (0.24 year−1) than in counties with smaller groves in hotspot areas (0.67 year−1), confirming reports that large-scale HLB management may slow epidemic progress.

scholarly journals A novel neural prosthesis providing long-term electrocorticography recording and cortical stimulation for epilepsy and brain-computer interface

2019 ◽  
Vol 130 (4) ◽  
pp. 1166-1179 ◽  
Author(s):  
Pantaleo Romanelli ◽  
Marco Piangerelli ◽  
David Ratel ◽  
Christophe Gaude ◽  
Thomas Costecalde ◽  
...  
Keyword(s):  
Nonhuman Primate ◽  
Large Scale ◽  
Brain Activity ◽  
Threshold Value ◽  
Time Span ◽  
Cortical Stimulation ◽  
Neural Prosthesis ◽  
Lower Limbs ◽  
Custom Made

OBJECTIVEWireless technology is a novel tool for the transmission of cortical signals. Wireless electrocorticography (ECoG) aims to improve the safety and diagnostic gain of procedures requiring invasive localization of seizure foci and also to provide long-term recording of brain activity for brain-computer interfaces (BCIs). However, no wireless devices aimed at these clinical applications are currently available. The authors present the application of a fully implantable and externally rechargeable neural prosthesis providing wireless ECoG recording and direct cortical stimulation (DCS). Prolonged wireless ECoG monitoring was tested in nonhuman primates by using a custom-made device (the ECoG implantable wireless 16-electrode [ECOGIW-16E] device) containing a 16-contact subdural grid. This is a preliminary step toward large-scale, long-term wireless ECoG recording in humans.METHODSThe authors implanted the ECOGIW-16E device over the left sensorimotor cortex of a nonhuman primate (Macaca fascicularis), recording ECoG signals over a time span of 6 months. Daily electrode impedances were measured, aiming to maintain the impedance values below a threshold of 100 KΩ. Brain mapping was obtained through wireless cortical stimulation at fixed intervals (1, 3, and 6 months). After 6 months, the device was removed. The authors analyzed cortical tissues by using conventional histological and immunohistological investigation to assess whether there was evidence of damage after the long-term implantation of the grid.RESULTSThe implant was well tolerated; no neurological or behavioral consequences were reported in the monkey, which resumed his normal activities within a few hours of the procedure. The signal quality of wireless ECoG remained excellent over the 6-month observation period. Impedance values remained well below the threshold value; the average impedance per contact remains approximately 40 KΩ. Wireless cortical stimulation induced movements of the upper and lower limbs, and elicited fine movements of the digits as well. After the monkey was euthanized, the grid was found to be encapsulated by a newly formed dural sheet. The grid removal was performed easily, and no direct adhesions of the grid to the cortex were found. Conventional histological studies showed no cortical damage in the brain region covered by the grid, except for a single microscopic spot of cortical necrosis (not visible to the naked eye) in a region that had undergone repeated procedures of electrical stimulation. Immunohistological studies of the cortex underlying the grid showed a mild inflammatory process.CONCLUSIONSThis preliminary experience in a nonhuman primate shows that a wireless neuroprosthesis, with related long-term ECoG recording (up to 6 months) and multiple DCSs, was tolerated without sequelae. The authors predict that epilepsy surgery could realize great benefit from this novel prosthesis, providing an extended time span for ECoG recording.

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