Combination of Bacillus licheniformis and Salinomycin: Effect on the Growth Performance and GIT Microbial Populations of Broiler Chickens

The aim of the study was to investigate the effect of Bacillus licheniformis and salinomycin supplementation in broiler diets as individual factors or in combination on the growth performance, GIT morphometry, and microbiota populations. Four hundred one-day-old Ross 308 chicks were randomly distributed to four dietary treatments (10 replicates, 10 birds each). The following treatments were applied: NC—no additives; NC + SAL—salinomycin addition (60 mg/kg diet), NC + PRO—B. licheniformis DSM 28710 preparation (1.6 × 109 CFU/kg; 500 mg/kg diet), and NC + SAL + PRO—combination of salinomycin and B. licheniformis. Probiotic administration resulted in improvement (p < 0.05) of the performance parameters, including body weight gain (1–10 d, and 11–22 d) and feed conversion ratio (11–22 d, 1–36 d). An interaction (p < 0.05) between experimental factors was observed in terms of lower pH values in the crop (tendency, p = 0.053) and ceca. Both factors lowered the alpha diversity and Enterobacteriaceae and promoted Bacillaceae communities in the jejunum (p < 0.05). Interactions were also observed in terms of reducing Clostridiaceae in the ceca. In conclusion, the combined use of B. licheniformis and salinomycin in broilers’ diets had beneficial effects.

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Effect of processed doum palm (Hyphaene thebaica) pulp meal on growth performance, nutrient digestibility, carcass characteristics and haematological indices of broilers

Journal of Animal Science and Veterinary Medicine ◽

10.31248/jasvm2021.292 ◽

2021 ◽

pp. 182-187

Author(s):

Ibe Emmanuel Agwor ◽

◽

Kudu Yahaya Salihu ◽

Bisi Alex Ayanwale ◽

Malik Abdulganiyu Ayodele ◽

...

Keyword(s):

Growth Performance ◽

Broiler Chickens ◽

Body Weight Gain ◽

Carcass Characteristics ◽

Nutrient Digestibility ◽

Feed Conversion ◽

Treatment Groups ◽

Carcass Parameters ◽

Dietary Treatments ◽

Haematological Indices

The increase in human population, particularly in the developing countries like Nigeria and the demand for animal protein intake has necessitated different approaches of solving protein shortage. A 28-day study was carried out to determine the performance of finishers fed on different hourly soaked doum palm (Hyphaene thebaica) pulp meal (DPPM) diets. Two hundred and sixteen (216) finishers (4 week-old) were randomly allotted to six dietary treatments. Each treatment consisted of 36 birds with three replicates (12 birds per replicate) in a completely randomized design (CRD). Six experimental diets were formulated and designed as follows: T1 (Control) contained 0% DPPM while T2, T3, T4, T5 and T6 contained 12.5% each of 2, 4, 6, 8 and 10 hours soaked DPPM as substitute for maize, respectively. Feeds and water were provided ad libitum for all treatment groups. The average body weight gain (40.44- 42.71 g/b/d), average feed intake (87.52-96.73 g/b/d) and feed conversion ratio (2.14-2.34) were non-significant (p>0.05) regardless of the dietary treatments. Statistical differences (p<0.05) were observed in some carcass parameters such as thigh (13.40-14.98%), neck (5.32-6.55%), shank (3.81-5.2 %), wing (9.80-11.41%), head (2.43-3.74%) and two haematological indices; white blood cell (160.67-189.53 X 103/mm3) and eosinophils (0.33-2.00%) across the treatment groups. The processed DPPM could serve as a rich energy source in broiler feeds with the raw doum palm pulp containing 2987.45 kcal/kg. Also, soaking the pulp for 2 to 10 hours before been included in broiler diet improved the nutritional content, acceptability, palatability and utilization of the feed by the broilers. Thus, up to 12.5% processed DPPM can be included in the diets of broiler chickens without adverse effect on growth performance, nutrient digestibility, carcass characteristics and haematological indices of chickens.

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Protected Organic Acids Improved Growth Performance, Nutrient Digestibility, and Decreased Gas Emission in Broilers

Animals ◽

10.3390/ani10030416 ◽

2020 ◽

Vol 10 (3) ◽

pp. 416 ◽

Cited By ~ 1

Author(s):

Dinh Hai Nguyen ◽

In Ho Kim

Keyword(s):

Organic Acids ◽

Growth Performance ◽

Broiler Chickens ◽

Body Weight Gain ◽

Basal Diet ◽

Nutrient Digestibility ◽

Broiler Chicks ◽

Feed Conversion ◽

Gas Emission ◽

Dietary Treatments

We investigated the effects of a blend of organic acids (OAs) and medium-chain fatty acids (MCFAs) supplementation in 800 1-d-old male Ross 308 broiler chickens (42 ± 0.90 g) in a 7-week study. Broiler chicks were randomly allocated into one of the five dietary treatments (16 birds per pen with 10 pens per treatment). Dietary treatments consisted of corn-soybean meal based basal diet and the basal diet supplemented with blend of OAs and MCFAs at 0.25, 0.5, 0.75 g, and 1 g per kg of feed. In the current study, during the whole experimental period, the inclusion of the blend of OAs and MCFAs in the basal diet linearly improved (p < 0.05) body weight gain (BWG), feed conversion ratio (FCR), and dry matter digestibility. The increasing inclusion of the blend of OA and MCFA levels in the diets linearly decreased (p = 0.002) feed intake during d 1 to 7. Broilers fed diets containing different levels of the blend of OAs and MCFAs showed a linear increase (p = 0.006) in Lactobacillus concentrations and decrease (p = 0.014) in ammonia (NH3) at the end of the experiment. However, the blend of OAs and MCFAs did not affect carcass quality, E. coli, and Salmonella counts, as well as hydrogen sulfide and total mercaptans gas emission (p > 0.05). In conclusion, the blend of OA and MCFA supplementation positively influenced growth performance, DM digestibility, excreta Lactobacillus counts, as well as NH3 gas emission in broiler chickens.

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Sorghum and wheat differentially affect caecal microbiota and associated performance characteristics of meat chickens

PeerJ ◽

10.7717/peerj.3071 ◽

2017 ◽

Vol 5 ◽

pp. e3071 ◽

Cited By ~ 13

Author(s):

Eduardo Crisol-Martínez ◽

Dragana Stanley ◽

Mark S. Geier ◽

Robert J. Hughes ◽

Robert J. Moore

Keyword(s):

Microbial Community ◽

Growth Performance ◽

Broiler Chickens ◽

Body Weight Gain ◽

Rrna Gene ◽

Performance Measurements ◽

Feed Conversion ◽

Nutritional Factors ◽

Dietary Treatments ◽

Caecal Microbiota

This study compared the effects of wheat- and sorghum-based diets on broiler chickens. The growth performance and caecal microbial community of chickens were measured and correlations between productivity and specific gut microbes were observed. Cobb broilers 15 days of age were individually caged and two dietary treatments were used, one with a wheat-based diet (n = 48) and another one with a sorghum-based diet (n = 48). Growth performance measurements were taken over a 10 day period and samples for microbiota analysis were taken at the end of that period. Caecal microbiota was characterised by sequencing of 16S bacterial rRNA gene amplicons. Overall, the results indicated that a sorghum-based diet produced higher apparent metabolisable energy (AME) and body-weight gain (BWG) values in chickens, compared to a wheat-based diet. Nevertheless, sorghum-fed birds had higher feed conversion ratio (FCR) values than wheat-fed birds, possibly because of some anti-nutritional factors in sorghum. Further analyses showed that caecal microbial community was significantly associated with AME values, but microbiota composition differed between dietary treatments. A number of bacteria were individually correlated with growth performance measurements. Numerous OTUs assigned to strains ofLactobacillus crispatusand Lachnospiraceae, which were prevalent in sorghum-fed chickens, were correlated with high AME and BWG values, respectively. Additionally, a number of OTUs assigned to Clostridiales that were prevalent in wheat-fed chickens were correlated with low FCR values. Overall, these results suggest that between-diet variations in growth performance were partly associated with changes in the caecal microbiota.

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Supplementing Garlic Nanohydrogel Optimized Growth, Gastrointestinal Integrity and Economics and Ameliorated Necrotic Enteritis in Broiler Chickens Using a Clostridium perfringens Challenge Model

Animals ◽

10.3390/ani11072027 ◽

2021 ◽

Vol 11 (7) ◽

pp. 2027

Author(s):

Doaa Ibrahim ◽

Tamer Ahmed Ismail ◽

Eman Khalifa ◽

Shaimaa A. Abd El-Kader ◽

Dalia Ibrahim Mohamed ◽

...

Keyword(s):

Growth Performance ◽

Clostridium Perfringens ◽

Broiler Chickens ◽

Body Weight Gain ◽

Economic Benefits ◽

Production Costs ◽

Broiler Chicks ◽

Necrotic Enteritis ◽

Feed Conversion ◽

Intestinal Lesion

Necrotic enteritis (NE) caused by Clostridium perfringens (C. perfringens) results in impaired bird growth performance and increased production costs. Nanotechnology application in the poultry industry to control NE outbreaks is still not completely clarified. Therefore, the efficacy of dietary garlic nano-hydrogel (G-NHG) on broilers growth performance, intestinal integrity, economic returns and its potency to alleviate C. perfringens levels using NE challenge model were addressed. A total of 1200 male broiler chicks (Ross 308) were assigned into six groups; four supplemented with 100, 200, 300 or 400 mg of G-NHG/kg diet and co-challenged with C. perfringens at 21, 22 and 23 d of age and two control groups fed basal diet with or without C. perfringens challenge. Over the total growing period, the 400 mg/kg G-NHG group had the most improved body weight gain and feed conversion efficiency regardless of challenge. Parallel with these results, the mRNA expression of genes encoding digestive enzymes (alpha 2A amylase (AMY2A), pancreatic lipase (PNLIP) and cholecystokinin (CCK)) and intestinal barriers (junctional adhesion molecule-2 (JAM-2), occludin and mucin-2 (Muc-2)) were increased in groups fed G-NHG at higher levels to be nearly similar to those in the unchallenged group. At 14 d post challenge, real-time PCR results revealed that inclusion of G-NHG led to a dose-dependently decrease in the C. perfringens population, thereby decreasing the birds’ intestinal lesion score and mortality rates. Using 400 mg/kg of G-NHG remarkably ameliorated the adverse effects of NE caused by C. perfringens challenge, which contributed to better growth performance of challenged birds with rational economic benefits.

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Effects of Lactobacillus reuteri and Streptomyces coelicolor on Growth Performance of Broiler Chickens

Microorganisms ◽

10.3390/microorganisms9061341 ◽

2021 ◽

Vol 9 (6) ◽

pp. 1341

Author(s):

Sarayu Bhogoju ◽

Collins N. Khwatenge ◽

Thyneice Taylor-Bowden ◽

Gabriel Akerele ◽

Boniface M. Kimathi ◽

...

Keyword(s):

Broiler Chickens ◽

Lactobacillus Reuteri ◽

Body Weight Gain ◽

Streptomyces Coelicolor ◽

Control Diet ◽

Probiotic Bacteria ◽

Feed Consumption ◽

Feed Conversion ◽

Wheat Middlings ◽

Dietary Treatments

There are well documented complications associated with the continuous use of antibiotics in the poultry industry. Over the past few decades, probiotics have emerged as viable alternatives to antibiotics; however, most of these candidate probiotic microorganisms have not been fully evaluated for their effectiveness as potential probiotics for poultry. Recent evaluation of a metagenome of broiler chickens in our laboratory revealed a prevalence of Lactobacillus reuteri (L. reuteri) and Actinobacteria class of bacteria in their gastrointestinal tract. In this study Lactobacillus reuteri and Streptomyces coelicolor (S. coelicolor) were selected as probiotic bacteria, encapsulated, and added into broiler feed at a concentration of 100 mg/kg of feed. In an 8-week study, 240 one day-old chicks were randomly assigned to four dietary treatments. Three dietary treatments contained two probiotic bacteria in three different proportions (L. reuteri and S. coelicolor individually at 100 ppm, and mixture of L. reuteri and S. coelicolor at 50 ppm each). The fourth treatment had no probiotic bacteria and it functioned as the control diet. L. reuteri and S. coelicolor were added to the feed by using wheat middlings as a carrier at a concentration of 100 ppm (100 mg/kg). Chickens fed diets containing L. reuteri and S. coelicolor mixture showed 2% improvement in body weight gain, 7% decrease in feed consumption, and 6–7% decrease in feed conversion ratios. This research suggests that L. reuteri and S. coelicolor have the potential to constitute probiotics in chickens combined or separately, depending on the desired selection of performance index.

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Kebutuhan Kalsium Ayam Broiler Fase Starter dengan Penambahan Enzim Fitase

Buletin Peternakan ◽

10.21059/buletinpeternak.v40i3.12491 ◽

2016 ◽

Vol 40 (3) ◽

pp. 170

Author(s):

Eny Sholikhatin ◽

Ferry Poernama ◽

Nanung Danar Dono ◽

Zuprizal .

Keyword(s):

Body Weight ◽

Growth Performance ◽

Feed Efficiency ◽

Broiler Chickens ◽

Bone Mineralization ◽

Body Weight Gain ◽

Basal Diet ◽

Beneficial Effects ◽

Randomized Design ◽

Bone Ash

The aim of this study was to determine the calcium requirements of broiler chickens in starter phase with the addition of phytase enzyme. This experiment used 1,200 broiler chickens (mixed sex) for 21 days rearing period in a closed house. The basal diets was corn and soybean meal which contained 0.097% Ca and 0.123% P-av. The treatments consisted of 6 diets treatment: P1 (basal diet with the addition of 0.42% non-phytate phosphorus (NPP), and then P2 to P6 were basal diet with the addition of 0.22% NPP and 1,000 FTU/kg phytase and the addition of calcium (Ca) at different levels: 0.82% (P2); 0.74% (P3); 0.66% (P4); 0.58% (P5) and 0.50% (P6). The growth performance, feed efficiency, and bone mineralization parameters were studied using Oneway ANOVA in a Completely Randomized Design. Duncan's new Multiple Ranges Test was used to separate means with significant differences. Results showed that 0.90% Ca supplementation without phytase and the reduction levels of Ca from 0.82% to 0.50% increased the amount of feed consumed by birds in all growth phases (P<0.05). The addition of phytase had beneficial effects on increasing body weight (P<0.05) and average of body weight gain (P<0.05), and decreased the value of FCR in 11 - 21 days and 1 - 21 days. The addition of phytase increased protein and energy consumption, followed by increased in the value of PER and EER (P<0.05). When phytase was added in the diets, reduction levels of Ca in the diets up to 0.50% did not give any adverse effect on the tibia bone ash. It can be concluded that feeding with phytase can sustain growth performance, feed efficiency, and bone mineralization.

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Effect of dietary supplementation of vitamin E, zinc, and chromium supplementation on growth performance and hematological characteristics of broiler chickens

Indian Journal of Animal Research ◽

10.18805/ijar.b-3293 ◽

2017 ◽

Author(s):

Nazim Ali ◽

Mohammad Akram ◽

Ahmad Fahim ◽

Balvir Singh ◽

Mohammad Imran

Keyword(s):

Body Weight ◽

Vitamin E ◽

Growth Performance ◽

Broiler Chickens ◽

Body Weight Gain ◽

White Blood Cells ◽

The Body ◽

Broiler Chicks ◽

Feed Conversion ◽

Wood Shavings

The study investigated the effect of supplementation of Vitamin E, Zn and Cr on growth performance and hematological characteristics of broiler chickens. The 240 day old Cobb 400 chicks were randomly distributed in eight groups consisting of 30 chicks in each. These chicks were kept on deep litter houses comprising of wood shavings. The supplementation of Vitamin E, zinc and chromium either individually or in combination improved the feed conversion ratio and body weight gain in broiler chicks. The body weight of broiler chickens at different age from day old to the 6th week were significantly different among treatments. The highest body weight was recorded in T8 groups. Heamoglobin, red blood corpuscles and white blood cells levels increased whereas heterophil: lymphocyte ratio statistically decreased with vitamin E, zinc or chromium supplemented groups either individually or in combinations.

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Evaluation of feeding value of rice based Distillers Dried Grains with Solubles (DDGS) for broiler chickens

Indian Journal of Animal Research ◽

10.18805/ijar.b-3607 ◽

2018 ◽

Author(s):

O. P. Dinani ◽

Pramod Kumar Tyagi ◽

A. B. Mandal ◽

Praveen Kumar Tyagi ◽

Narayan Dutta

Keyword(s):

Growth Performance ◽

Broiler Chickens ◽

Body Weight Gain ◽

Nitrogen Retention ◽

Live Weight ◽

Nutrient Utilization ◽

Distillers Dried Grains ◽

Inclusion Level ◽

Dietary Treatments

A biological experiment of 160 day old chicks for six weeks was undertaken with completely randomized design (CRD) divided into five treatments, each consisting of 4 replicates and 8 chicks in each replicate. Five experimental diets as per ICAR (2013) were prepared by incorporating rice distillers dried grains with solubles (rDDGS) at inclusion level of (0, 7.5, 10.0, 12.5 and 15.0%). Chemical analysis revealed that rice DDGS on as such basis is high in both crude protein 44.68% and gross energy 4232 kcal/kg. In vitro pepsin-pancreatin digestibility (IVPPD) of rDDGS was found 79.81%. At the inclusion level of 7.5, 10 and 12.5 %, growth performance and nutrient utilization did not show any significant (P>0.05) difference as compared to control but at 15% rDDGS inclusion level, growth performance (body weight gain, feed intake and FCR) and nutrient utilization (dry matter and energy metabolizability, nitrogen retention %) were significantly (Pless than0.01) lower in 15% rDDGS (T5) group as compared to control (T1) and other dietary treatments. Carcass traits did not show any significant (P>0.05) difference between control and other dietary treatments. Cost of production per kg live weight and meat yield were significantly (Pless than 0.01) lower in 7.5 % and above rDDGS levels. Thus, it is concluded that rDDGS can be safely incorporated in broiler chickens diets up to the inclusion level of 12.5% as alternate protein meal for economic broiler production.

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L-carnitine supplementation decreases lipid peroxidation and improves cardiopulmonary function in broiler chickens reared at high altitude

Acta Veterinaria Hungarica ◽

10.1556/avet.2014.022 ◽

2014 ◽

Vol 62 (4) ◽

pp. 489-499 ◽

Cited By ~ 5

Author(s):

Fariborz Khajali ◽

Zahra Khajali

Keyword(s):

Lipid Peroxidation ◽

High Altitude ◽

Broiler Chickens ◽

Body Weight Gain ◽

Weight Ratio ◽

Control Group ◽

Carnitine Supplementation ◽

Feed Conversion ◽

Cardiopulmonary Function ◽

Beneficial Effects

An experiment was carried out to examine the effects of L-carnitine supplementation on growth performance and cardiopulmonary function of broiler chickens reared at high altitude (2100 m above sea level). A total of 96 day-old male chicks (Cobb 500) were randomly assigned into two dietary treatments containing 0 (control group) and 200 mg/kg L-carnitine. The experimental diets were fed for a period of 42 days consisting of the starting (days 1 to 21) and growing periods (days 21 to 42). Nutrient requirements of chickens met the NRC (1994) recommendations. The results showed that dietary L-carnitine had no significant influence on body weight gain, feed intake and feed conversion ratio. L-carnitine reduced plasma concentration of malondialdehyde, packed cell volume (PCV) and abdominal fat deposition compared to the control (P < 0.05). A significant (P < 0.05) decrease was observed in the right to total ventricular weight ratio (RV:TV) in birds receiving L-carnitine when compared to the control. Supplementation of L-carnitine increased plasma nitric oxide and immune responsiveness, which manifested in an increased toe-web thickness index measured at 24 h following the injection of phytohaemagglutinin P. In conclusion, supplementation of L-carnitine had beneficial effects on preventing lipid peroxidation and pulmonary hypertension in broiler chickens raised at high altitude.

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Effect of Dietary Modulation of Selenium Form and Level on Performance, Tissue Retention, Quality of Frozen Stored Meat and Gene Expression of Antioxidant Status in Ross Broiler Chickens

Animals ◽

10.3390/ani9060342 ◽

2019 ◽

Vol 9 (6) ◽

pp. 342 ◽

Cited By ~ 6

Author(s):

Doaa Ibrahim ◽

Asmaa T.Y. Kishawy ◽

Safaa I. Khater ◽

Ahmed Hamed Arisha ◽

Haiam A. Mohammed ◽

...

Keyword(s):

Growth Performance ◽

Broiler Chickens ◽

Body Weight Gain ◽

Frozen Storage ◽

Storage Period ◽

Dietary Supplementation ◽

Broiler Chicks ◽

Feed Conversion ◽

Fresh State

This study compares between different selenium forms (sodium selenite; SeS, selenomethionine; Met-Se or nano-Se) and levels on growth performance, Se retention, antioxidative potential of fresh and frozen meat, and genes related to oxidative stress in Ross broilers. Birds (n = 450) were randomly divided into nine experimental groups with five replicates in each and were fed diets supplemented with 0.3, 0.45, and 0.6 mg Se/kg as (SeS, Met-Se), or nano-Se. For overall growth performance, dietary inclusion of Met-Se or nano-Se significantly increased (p < 0.05) body weight gain and improved the feed conversion ratio of Ross broiler chicks at the level of 0.45 and 0.6 mg/kg when compared with the group fed the same level of SeS. Se sources and levels significantly affected (p < 0.05) its concentrations in breast muscle, liver, and serum. Moreover, Se retention in muscle was higher (p < 0.05) after feeding of broiler chicks on a diet supplemented with Met-Se or nano-Se compared to the SeS group, especially at 0.6 mg/kg. Additionally, higher dietary levels from Met-Se or nano-Se significantly reduced oxidative changes in breast and thigh meat in the fresh state and after a four-week storage period and increased muscular pH after 24 h of slaughter. Also, broiler’s meat in the Met-Se and nano-Se groups showed cooking loss and lower drip compared to the SeS group (p < 0.05). In the liver, the mRNA expression levels of glutathione peroxidase, superoxide dismutase, and catalase were elevated by increasing dietary Se levels from Met-Se and nano-Se groups up to 0.6 mg/kg when compared with SeS. Therefore, dietary supplementation with 0.6 mg/kg Met-Se and nano-Se improved growth performance and were more efficiently retained than with SeS. Both sources of selenium (Met-Se and nano-Se) downregulated the oxidation processes of meat during the first four weeks of frozen storage, especially in thigh meat, compared with an inorganic source. Finally, dietary supplementation of Met-Se and nano-Se produced acceptable Se levels in chicken meat offered for consumers.

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