scholarly journals Synovial Concentration of Trimethoprim-Sulphadiazine Following Regional Limb Perfusion in Standing Horses

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2085
Author(s):  
Kajsa Gustafsson ◽  
Amos J. Tatz ◽  
Roee Dahan ◽  
Wiessam Abu Ahmad ◽  
Malka Britzi ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Adverse Effects ◽  
Inhibitory Concentration ◽  
Cephalic Vein ◽  
Routes Of Administration ◽  
Chromatography Tandem Mass Spectrometry ◽  
Limb Perfusion ◽  
Low Concentrations ◽  
Liquid Chromatography Tandem Mass ◽  
Over Time

The aim of this study was to investigate the safety and pharmacokinetics of trimethoprim-sulphadiazine administered via intravenous regional limb perfusion (IVRLP) into the cephalic vein. According to the hypothesis, the drug could be administered without adverse effects and the synovial concentrations would remain above the minimum inhibitory concentration (MIC) for trimethoprim-sulphadiazine (0.5 and 9.5 µg/mL) for 24 h. Ten (n = 10) horses underwent cephalic vein IVRLP with an Esmarch tourniquet applied for 30 min. Four grams (4 g) of trimethoprim-sulphadiazine (TMP-SDZ) were diluted at 0.9% NaCl for a total volume of 100 mL. Synovial fluid and blood samples were obtained immediately before IVRLP and at 0.25, 0.5, 2, 6, 12 and 24 h after the initiation of IVRLP. Trimethoprim and sulphadiazine concentrations were determined using a method based on liquid chromatography/tandem mass spectrometry. The Cmax (peak drug concentration) values were 36 ± 31.1 and 275.3 ± 214.4 µg/mL (TMP and SDZ). The respective tmax (time to reach Cmax) values were 20 ± 7.8 and 26.4 ± 7.2 min. The initial synovial fluid concentrations were high but decreased quickly. No horse had synovial concentrations of trimethoprim-sulphadiazine above the MIC at 12 h. Severe vasculitis and pain shortly after IVRLP, lasting up to one week post-injection, occurred in five out of 10 horses. In conclusion, IVRLP with trimethoprim-sulphadiazine cannot be recommended due to the low concentrations of synovial fluid over time and the frequent severe adverse effects causing pain and discomfort in treated horses. Thus, in cases of septic synovitis with bacteria sensitive to trimethoprim-sulphadiazine, other routes of administration should be considered.

scholarly journals Quantification of peptides in human synovial fluid using liquid chromatography–tandem mass spectrometry

Talanta ◽  
2018 ◽  
Vol 186 ◽  
pp. 124-132 ◽  
Author(s):  
Araceli Garcia-Ac ◽  
Sung Vo Duy ◽  
Sébastien Sauvé ◽  
Florina Moldovan ◽  
V. Gaëlle Roullin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Synovial Fluid ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Human Synovial Fluid

scholarly journals Determination of macroline and tetracyclines group in water by liquid chromatography tandem mass spectrometry (LC-MS/MS)

2020 ◽  
Vol 3 (3) ◽  
pp. 173-182
Author(s):  
Trong Le Van ◽  
Thanh Hang Nguyen Thi ◽  
Huy Do Quang ◽  
Khai Nguyen Manh ◽  
◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Accuracy ◽  
Method Detection Limit ◽  
Chromatography Tandem Mass Spectrometry ◽  
Low Concentrations ◽  
Liquid Chromatography Tandem Mass ◽  
Trace Concentrations ◽  
Positive Ion ◽  
Tetracycline Group

Antibiotics are contaminants at low concentrations and found in water with the range of ng/L. Determination of antibiotics at trace concentrations requires modern and high accuracy equipment. Liquid chromatography mass tandem spectrometry (LC-MS/MS) able to determine contaminants at ppt and ppb levels, which is suitable for quantification of the macrolide and tetracycline group in water. The Analytical method using Agilent Triple Quard 6460 LC-MS/MS system with positive ion mode. Agilent XDB C18 column (1.8 µm × 2.1 × 100 mm) and Agilent Eclipse SDB C18 pre-column (2.1 × 5 mm × 1.8 µm) were used. Mobile phase was acetonitrile and HCOOH 0.1%. Samples were cleaned with Oasis PRiME HLB 3cc SPE column (150 mg). The method was evaluated based on specificity, recovery, repeatability and estimation of the uncertainty. The method detection limit (MDL) for the analytes is 0.03 µg/L. Recovery ranges from 85.23 -117.70%; repeatability (RSDr) is between 2.20% and 12.45%.

scholarly journals Time to Peak Concentration of Amikacin in the Antebrachiocarpal Joint Following Cephalic Intravenous Regional Limb Perfusion in Standing Horses

2020 ◽  
Vol 33 (05) ◽  
pp. 327-332
Author(s):  
Kajsa Gustafsson ◽  
Amos J. Tatz ◽  
Roee Dahan ◽  
Malka Britzi ◽  
Stefan Soback ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Healthy Adult ◽  
Time To Peak ◽  
Limb Perfusion ◽  
Mixed Breed ◽  
Radiocarpal Joint ◽  
Liquid Chromatography Tandem Mass ◽  
Antibiotic Solution ◽  
23 Gauge ◽  
Mean Concentration

Abstract Objective The aim of this study was to determine the time (Tmax) to the maximum concentration (Cmax) of amikacin sulphate in synovial fluid of the radiocarpal joint (RCJ) following cephalic intravenous regional limb perfusion (IVRLP) using 2 g of amikacin sulphate. Methods Cephalic IVRLP was performed with 2 g of amikacin sulphate diluted in 0.9% NaCl to a total volume of 100 mL in six healthy adult mixed breed mares. An Esmarch's rubber tourniquet was applied for 30 minutes and the antibiotic solution was infused through a 23-gauge butterfly catheter. Synovial fluid was collected from the RCJ prior to the infusion and at 5, 10, 15, 20, 25 and 30 minutes after completion of IVRLP. The tourniquet was removed after the last arthrocentesis. Synovial fluid amikacin sulphate concentrations were determined by liquid chromatography/tandem mass spectrometry. Results The calculated mean Tmax occurred at 15 minutes (range: 10–20 minutes) post-perfusion. The highest synovial fluid amikacin sulphate concentration was noted at 10 minutes in 2 horses, 15 minutes in 2 horses and 20 minutes in 2 horses. The highest mean concentration was 1023 µg/mL and was noted at 20 minutes. Synovial mean concentrations were significantly different between 15 and 30 minutes. Clinical Significance In this study no Tmax occurred after 20 minutes; thus, 30 minutes of tourniquet application time appear to be excessive. The 20 minutes duration of tourniquet application appears sufficient for the treatment of the RCJ in cephalic IVRLP using 2 g amikacin sulphate in a total volume of 100 mL.

scholarly journals Stability Evaluation of DMT and Harmala Alkaloids in Ayahuasca Tea Samples

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2072 ◽  
Author(s):  
Gabriela de Oliveira Silveira ◽  
Rafael Guimarães dos Santos ◽  
Felipe Rebello Lourenço ◽  
Giordano Novak Rossi ◽  
Jaime Hallak ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Stability Evaluation ◽  
Glass Containers ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Harmala Alkaloids ◽  
Health Disorders ◽  
The Stability ◽  
Over Time

Ayahuasca tea is a hallucinogenic beverage used for religious purposes in Brazil and many other countries that has therapeutic potential in the treatment of some mental health disorders. In the context of psychedelic research, quantification of the tea’s main alkaloids prior to its administration in animal or human studies is essential. For this reason, this study aims to provide information regarding the stability of the main ayahuasca alkaloids (dimethyltryptamine, DMT; harmine, HRM; tetrahydroharmine, THH; harmaline, HRL) in three different conditions: (1) A year stored in a refrigerator either in plastic or glass containers, (2) seven days at 37 °C to reproduce usual mail transportation, and (3) after three freeze–thaw cycles. Samples were quantified after a dilute-and-shoot procedure using liquid chromatography tandem mass spectrometry (LC-ESI-MS/MS). There was no significant degradation of DMT concentration over time in all tested conditions. Harmala alkaloids (THH, HRL, and HRM) showed important variations after long-term and high-temperature storages. Although DMT has proven to be stable in all studied conditions, the harmala alkaloids revealed intense degradation and even concentration increment. This may be caused by degradation, alkaloid inter-conversion, and leaching from tea precipitate material. Therefore, ayahuasca quantification before administration in controlled sets is mandatory.

Fluorous solid-phase extraction (F-SPE) as a pilot tool for quantitative determination of perfluorochemicals in water samples coupled with liquid chromatography-tandem mass spectrometry

RSC Advances ◽  
2015 ◽  
Vol 5 (17) ◽  
pp. 13192-13199 ◽  
Author(s):  
Chen Xu ◽  
Jiajun Zhu ◽  
Yan Li ◽  
Yingjia Yu ◽  
Gengli Duan
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Adverse Effects ◽  
Solid Phase ◽  
Tandem Mass ◽  
Phase Extraction ◽  
Organofluorine Compounds ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

As a large group of stable existing organofluorine compounds widely present in the environment, perfluorochemicals (PFCs) could pose potential adverse effects on human health.

Failure of Intraarticular Ceftazidime in a Patient with Pseudomonas Aeruginosa Septic Arthritis

1988 ◽  
Vol 22 (4) ◽  
pp. 315-316 ◽  
Author(s):  
Elizabeth A. Kay ◽  
Peter S. Klimiuk ◽  
Gareth Ronson ◽  
George R. Bailie
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Minimum Inhibitory Concentration ◽  
Synovial Fluid ◽  
Septic Arthritis ◽  
Inhibitory Concentration ◽  
Single Patient ◽  
Patient Study ◽  
Routes Of Administration

This report describes a patient with Pseudomonas aeruginosa septic arthritis who received ceftazidime by the intravenous and intraarticular routes. Concentrations of ceftazidime in the synovial fluid following both routes of administration were measured and found to be above the minimum inhibitory concentration. Despite this the organism was not eradicated. We were unable to find other literature describing the disposition of ceftazidime in synovial fluid and therefore this single-patient study provides novel information. We were unable to account for the apparent failure of therapy.

scholarly journals Oral estrogen leads to falsely low concentrations of estradiol in a common immunoassay

2022 ◽  
Author(s):  
Lauren R. Cirrincione ◽  
Bridgit O. Crews ◽  
Jane A. Dickerson ◽  
Matthew D. Krasowski ◽  
Jessica Rongitsch ◽  
...  
Keyword(s):  
Secondary Analysis ◽  
Transgender Women ◽  
Estradiol Treatment ◽  
Cross Sectional ◽  
Chromatography Tandem Mass Spectrometry ◽  
Low Concentrations ◽  
Care Approach ◽  
Liquid Chromatography Tandem Mass ◽  
Roche Diagnostics ◽  
Estradiol Concentration

Objectives: Recently, an estradiol immunoassay manufacturer (Beckman Coulter, USA) issued an “Important Product Notice” alerting clinical laboratories their assay (Access Sensitive Estradiol) was not indicated for patients undergoing exogenous estradiol treatment. The objective of this analysis was to evaluate immunoassay bias relative to liquid chromatography tandem mass spectrometry (LC-MS/MS) in transgender women and to examine the influence of unconjugated estrone on measurements. Design: Cross sectional secondary analysis. Methods: Estradiol concentrations from 89 transgender women were determined by three immunoassays (Access Sensitive Estradiol [‘New BC’] and Access Estradiol assays [‘Old BC’], Beckman Coulter; Estradiol III assay [‘Roche’], Roche Diagnostics) and LC-MS/MS. Bias was evaluated with and without adjustment for estrone concentrations. The number of participants who shifted between three estradiol concentration ranges for each immunoassay versus LC-MS/MS (>300 pg/mL, 70-300 pg/mL, and <70 pg/mL) was calculated. Results: The New BC assay had the largest magnitude overall bias (median: -34%) and was -40%, -22%, and -10%, among participants receiving tablet, patch, or injection preparations, respectively. Overall bias was -12% and +17% for the Roche and Old BC assays, respectively. When measured with the New BC assay, 18 participants shifted to a lower estradiol concentration range (versus 9 and 10 participants based on Roche or Old BC assays, respectively). Adjustment for estrone did not minimize bias. Conclusions: Immunoassay measurement of estradiol in transgender women may lead to falsely decreased concentrations that have the potential to affect management. A multi-disciplinary health care approach is needed to ensure appropriate analytical methods are available.

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