scholarly journals Oral estrogen leads to falsely low concentrations of estradiol in a common immunoassay

2022 ◽  
Author(s):  
Lauren R. Cirrincione ◽  
Bridgit O. Crews ◽  
Jane A. Dickerson ◽  
Matthew D. Krasowski ◽  
Jessica Rongitsch ◽  
...  
Keyword(s):  
Secondary Analysis ◽  
Transgender Women ◽  
Estradiol Treatment ◽  
Cross Sectional ◽  
Chromatography Tandem Mass Spectrometry ◽  
Low Concentrations ◽  
Care Approach ◽  
Liquid Chromatography Tandem Mass ◽  
Roche Diagnostics ◽  
Estradiol Concentration

Objectives: Recently, an estradiol immunoassay manufacturer (Beckman Coulter, USA) issued an “Important Product Notice” alerting clinical laboratories their assay (Access Sensitive Estradiol) was not indicated for patients undergoing exogenous estradiol treatment. The objective of this analysis was to evaluate immunoassay bias relative to liquid chromatography tandem mass spectrometry (LC-MS/MS) in transgender women and to examine the influence of unconjugated estrone on measurements. Design: Cross sectional secondary analysis. Methods: Estradiol concentrations from 89 transgender women were determined by three immunoassays (Access Sensitive Estradiol [‘New BC’] and Access Estradiol assays [‘Old BC’], Beckman Coulter; Estradiol III assay [‘Roche’], Roche Diagnostics) and LC-MS/MS. Bias was evaluated with and without adjustment for estrone concentrations. The number of participants who shifted between three estradiol concentration ranges for each immunoassay versus LC-MS/MS (>300 pg/mL, 70-300 pg/mL, and <70 pg/mL) was calculated. Results: The New BC assay had the largest magnitude overall bias (median: -34%) and was -40%, -22%, and -10%, among participants receiving tablet, patch, or injection preparations, respectively. Overall bias was -12% and +17% for the Roche and Old BC assays, respectively. When measured with the New BC assay, 18 participants shifted to a lower estradiol concentration range (versus 9 and 10 participants based on Roche or Old BC assays, respectively). Adjustment for estrone did not minimize bias. Conclusions: Immunoassay measurement of estradiol in transgender women may lead to falsely decreased concentrations that have the potential to affect management. A multi-disciplinary health care approach is needed to ensure appropriate analytical methods are available.

Serum diiodotyrosine—a biomarker to differentiate destructive thyroiditis from Graves’ disease

2021 ◽  
Author(s):  
Naoya Fujita ◽  
Yosuke Ono ◽  
Azusa Sano ◽  
Motohiro Kimata ◽  
Seigo Oyama ◽  
...  
Keyword(s):  
Roc Curve ◽  
Operating Characteristic ◽  
Cross Sectional Study ◽  
Curve Analysis ◽  
Diagnostic Methods ◽  
Graves Disease ◽  
Roc Curve Analysis ◽  
Cross Sectional ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Objective: Conventional diagnostic methods are limited in their ability to differentiate destructive thyroiditis from Graves’ disease. We hypothesised that serum diiodotyrosine (DIT) and monoiodotyrosine (MIT) levels could be biomarkers for differentiating destructive thyroiditis from Graves’ disease. Design: Patients with destructive thyroiditis (n = 13) and Graves’ disease (n = 22) were enrolled in this cross-sectional study. Methods: We assayed the serum DIT and MIT levels using liquid chromatography-tandem mass spectrometry. A receiver operating characteristic (ROC) curve analysis was used to determine the sensitivity and specificity of the serum DIT and MIT levels as biomarkers for differentiating destructive thyroiditis from Graves’ disease. Results: The serum DIT and MIT levels were significantly higher in patients with destructive thyroiditis than in those with Graves’ disease. The ROC curve analysis showed that the serum DIT levels (≥ 359.9 pg/mL) differentiated destructive thyroiditis from Graves’ disease, significantly, with 100.0% sensitivity and 95.5% specificity (P < .001). The diagnostic accuracy of the serum MIT levels (≥119.4 pg/mL) was not as high as that of the serum DIT levels (sensitivity, 84.6%; specificity, 77.3%; P = .001). Conclusions: The serum DIT levels may serve as a novel diagnostic biomarker for differentiating destructive thyroiditis from Graves’ disease.

Individual-level characteristics associated with oral HIV test acceptability among Peruvian men who have sex with men and transgender women: a cross-sectional study

2018 ◽  
Vol 94 (7) ◽  
pp. 528-533 ◽  
Author(s):  
Juan A Flores ◽  
Brandon Brown ◽  
Segundo R León ◽  
Hugo Sánchez ◽  
Jerome T Galea
Keyword(s):  
Health Centre ◽  
Secondary Analysis ◽  
Prevalence Ratio ◽  
Transgender Women ◽  
Cross Sectional ◽  
Individual Level ◽  
Factors Associated ◽  
Hiv Test ◽  
Sex With Men ◽  
The Individual

ObjectivesPeruvian men who have sex with men (MSM) and transgender women (TGW) are highly vulnerable to HIV infection (HIV), but stigma, access issues and fear of venipuncture hamper testing. The oral HIV test—which uses oral fluids and provides results in 20 minutes—could reduce these barriers. The objective of this study was to determine the acceptability of the oral HIV test and the individual-level factors associated with its acceptability among MSM and TGW.MethodsWe conducted a cross-sectional secondary analysis among Peruvian MSM and TGW attending a community-based health centre between February 2012 and February 2013 to determine the individual-level factors associated with oral HIV test acceptability.ResultsOf 334 participants, 88% were MSM and 12% TGW. Overall, 85% of participants indicated their acceptability of the oral HIV test. Acceptability was higher in MSM than TGW (85.7% vs 80.0%) but this difference was not significant. Factors associated with acceptability in MSM were: tertiary or higher education (prevalence ratio (PR)=1.18, 95% CI 1.06 to 1.32 and PR=1.16, 95% CI 1.03 to 1.30, respectively); sex with drug use (PR=1.19, 95% CI 1.05 to 1.36); believing that HIV is transmitted by saliva (PR=1.20, 95% CI 1.08 to 1.33); and potential use of the oral test at home (PR=1.56, 95% CI 1.32 to 1.85). The only factor associated with lower acceptability was having had first anal intercourse between 14 and 19 years of age (PR=0.89, 95% CI 0.80 to 0.98).ConclusionsWe identified the individual factors associated with oral HIV test acceptability among Peruvian MSM and TGW. Expanded use of the oral HIV test to increase testing rates among Peruvian MSM and TGW is recommended.Trial registration numberNCT01387412, post-results.

scholarly journals Determination of macroline and tetracyclines group in water by liquid chromatography tandem mass spectrometry (LC-MS/MS)

2020 ◽  
Vol 3 (3) ◽  
pp. 173-182
Author(s):  
Trong Le Van ◽  
Thanh Hang Nguyen Thi ◽  
Huy Do Quang ◽  
Khai Nguyen Manh ◽  
◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Accuracy ◽  
Method Detection Limit ◽  
Chromatography Tandem Mass Spectrometry ◽  
Low Concentrations ◽  
Liquid Chromatography Tandem Mass ◽  
Trace Concentrations ◽  
Positive Ion ◽  
Tetracycline Group

Antibiotics are contaminants at low concentrations and found in water with the range of ng/L. Determination of antibiotics at trace concentrations requires modern and high accuracy equipment. Liquid chromatography mass tandem spectrometry (LC-MS/MS) able to determine contaminants at ppt and ppb levels, which is suitable for quantification of the macrolide and tetracycline group in water. The Analytical method using Agilent Triple Quard 6460 LC-MS/MS system with positive ion mode. Agilent XDB C18 column (1.8 &micro;m &times; 2.1 &times; 100 mm) and Agilent Eclipse SDB C18 pre-column (2.1 &times; 5 mm &times; 1.8 &micro;m) were used. Mobile phase was acetonitrile and HCOOH 0.1%. Samples were cleaned with Oasis PRiME HLB 3cc SPE column (150 mg). The method was evaluated based on specificity, recovery, repeatability and estimation of the uncertainty. The method detection limit (MDL) for the analytes is 0.03 &micro;g/L. Recovery ranges from 85.23 -117.70%; repeatability (RSDr) is between 2.20% and 12.45%.

scholarly journals Estrone Is a Strong Predictor of Circulating Estradiol in Women Age 70 Years and Older

2020 ◽  
Vol 105 (9) ◽  
pp. e3348-e3354
Author(s):  
Susan R Davis ◽  
Alejandra Martinez-Garcia ◽  
Penelope J Robinson ◽  
David J Handelsman ◽  
Reena Desai ◽  
...  
Keyword(s):  
Health Outcomes ◽  
Postmenopausal Women ◽  
Limit Of Detection ◽  
Strong Predictor ◽  
Serum Concentrations ◽  
Community Based ◽  
Cross Sectional ◽  
A Value ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Abstract Importance After menopause, estradiol (E2) is predominately an intracrine hormone circulating in very low serum concentrations. Objective The objective of this work is to examine determinants of E2 concentrations in women beyond age 70 years. Design and Setting A cross-sectional, community-based study was conducted. Participants A total of 5325 women participated, with a mean age of 75.1 years (± 4.2 years) and not using any sex steroid, antiandrogen/estrogen, glucocorticoid, or antiglycemic therapy. Main Outcome Measures Sex steroids were measured by liquid chromatography–tandem mass spectrometry. Values below the limit of detection (LOD; E2 11 pmol/L [3 pg/mL] were assigned a value of LOD/√2 to estimate total E2. Results E2 and estrone (E1) were below the LOD in 66.1% and 0.9% of women, respectively. The median (interdecile ranges) for E1 and detectable E2 were 181.2 pmol/L (range, 88.7-347.6 pmol/L) and 22.0 pmol/L (range, 11.0-58.7 pmol/L). Women with undetectable E2 vs detectable E2 were older (median age 74.1 years vs 73.8, P = .02), leaner (median body mass index [BMI] 26.8 kg/m2 vs 28.5, P &lt; .001), and had lower E1, testosterone and DHEA concentrations (P &lt; .001). A linear regression model, including age, BMI, E1, and testosterone, explained 20.9% of the variation in total E2, but explained only an additional 1.2% of variation over E1 alone. E1 and testosterone made significant contributions (r2 = 0.162, P &lt; .001) in a model for the subset of women with detectable E2. Conclusions Our findings support E1 as a principal circulating estrogen and demonstrate a robust association between E1 and E2 concentrations in postmenopausal women. Taken together with prior evidence for associations between E1 and health outcomes, E1 should be included in studies examining associations between estrogen levels and health outcomes in postmenopausal women.

scholarly journals Advances in Analysis and Detection of Major Mycotoxins in Foods

Foods ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 518 ◽  
Author(s):  
Sofia Agriopoulou ◽  
Eygenia Stamatelopoulou ◽  
Theodoros Varzakas
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Performance ◽  
Analytical Techniques ◽  
Molecular Techniques ◽  
Extraction Techniques ◽  
Detection Techniques ◽  
Chromatography Tandem Mass Spectrometry ◽  
Low Concentrations ◽  
Liquid Chromatography Tandem Mass

Mycotoxins are the most widely studied biological toxins, which contaminate foods at very low concentrations. This review describes the emerging extraction techniques and the current and alternatives analytical techniques and methods that have been used to successfully detect and identify important mycotoxins. Some of them have proven to be particularly effective in not only the detection of mycotoxins, but also in detecting mycotoxin-producing fungi. Chromatographic techniques such as high-performance liquid chromatography coupled with various detectors like fluorescence, diode array, UV, liquid chromatography coupled with mass spectrometry, and liquid chromatography-tandem mass spectrometry, have been powerful tools for analyzing and detecting major mycotoxins. Recent progress of the development of rapid immunoaffinity-based detection techniques such as immunoassays and biosensors, as well as emerging technologies like proteomic and genomic methods, molecular techniques, electronic nose, aggregation-induced emission dye, quantitative NMR and hyperspectral imaging for the detection of mycotoxins in foods, have also been presented.

Automated Measurement of 25-OH Vitamin D3 on the Roche Modular E170 Analyzer

2008 ◽  
Vol 54 (12) ◽  
pp. 2059-2062 ◽  
Author(s):  
Aila Leino ◽  
Ursula Turpeinen ◽  
Pertti Koskinen
Keyword(s):  
Vitamin D3 ◽  
Tandem Mass ◽  
Plasma Samples ◽  
Automated Measurement ◽  
Serum Samples ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Electrochemiluminescence Immunoassay ◽  
Automated Immunoassay ◽  
Roche Diagnostics

AbstractBackground: The first commercial direct automated immunoassay specific for 25-OH vitamin D3 (25-OH-D3) was recently introduced for use on Roche Diagnostics immunoassay analyzers. We assessed the analytical performance of the Elecsys 25-OH-D3 assay on a Roche Modular E 170 analyzer.Methods: The Elecsys 25-OH-D3 assay is a direct electrochemiluminescence immunoassay for human serum or plasma. It is a competitive assay in which the binding protein of vitamin D is inactivated during incubation. The assay employs a polyclonal antibody directed against 25-OH vitamin D3. We compared the 25-OH-D3 assay to assays performed with RIA, HPLC, and liquid chromatography–tandem mass spectrometry (LC-MS/MS).Results: At concentrations of 48, 76, and 124 nmol/L, within-run CVs were 5.1%, 3.1%, and 7.1% and total CVs were 12.1%, 7.4%, and 10.6%, respectively. A comparison of Elecsys 25-OH vitamin D3 with RIA yielded the regression equation: Elecsys = 1.114 × RIA – 6.15 (Sy|x = 15.7 nmol/L; n = 163). The corresponding equation with HPLC was: Elecsys = 1.077 × HPLC + 5.442 (Sy|x = 13.9 nmol/L; n = 67) and with LC-MS/MS: Elecsys = 0.887 × LC-MS/MS + 5.046 (Sy|x =12.4 nmol/L; n = 64). Contrary to LC-MS/MS, with the cutoff of 50 nmol/L (deficiency vs normal), approximately 10% of samples were misclassified as normal with RIA and Elecsys. Plasma samples were observed to have markedly higher concentrations than serum samples.Conclusions: The Elecsys concentrations of 25-OH-D3 were in good overall agreement with those determined with LC-MS/MS and RIA. However, large between-method variation was observed in individual patient samples. Use of serum rather than plasma is preferred owing to the higher results observed with plasma samples.

scholarly journals Synovial Concentration of Trimethoprim-Sulphadiazine Following Regional Limb Perfusion in Standing Horses

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2085
Author(s):  
Kajsa Gustafsson ◽  
Amos J. Tatz ◽  
Roee Dahan ◽  
Wiessam Abu Ahmad ◽  
Malka Britzi ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Adverse Effects ◽  
Inhibitory Concentration ◽  
Cephalic Vein ◽  
Routes Of Administration ◽  
Chromatography Tandem Mass Spectrometry ◽  
Limb Perfusion ◽  
Low Concentrations ◽  
Liquid Chromatography Tandem Mass ◽  
Over Time

The aim of this study was to investigate the safety and pharmacokinetics of trimethoprim-sulphadiazine administered via intravenous regional limb perfusion (IVRLP) into the cephalic vein. According to the hypothesis, the drug could be administered without adverse effects and the synovial concentrations would remain above the minimum inhibitory concentration (MIC) for trimethoprim-sulphadiazine (0.5 and 9.5 µg/mL) for 24 h. Ten (n = 10) horses underwent cephalic vein IVRLP with an Esmarch tourniquet applied for 30 min. Four grams (4 g) of trimethoprim-sulphadiazine (TMP-SDZ) were diluted at 0.9% NaCl for a total volume of 100 mL. Synovial fluid and blood samples were obtained immediately before IVRLP and at 0.25, 0.5, 2, 6, 12 and 24 h after the initiation of IVRLP. Trimethoprim and sulphadiazine concentrations were determined using a method based on liquid chromatography/tandem mass spectrometry. The Cmax (peak drug concentration) values were 36 ± 31.1 and 275.3 ± 214.4 µg/mL (TMP and SDZ). The respective tmax (time to reach Cmax) values were 20 ± 7.8 and 26.4 ± 7.2 min. The initial synovial fluid concentrations were high but decreased quickly. No horse had synovial concentrations of trimethoprim-sulphadiazine above the MIC at 12 h. Severe vasculitis and pain shortly after IVRLP, lasting up to one week post-injection, occurred in five out of 10 horses. In conclusion, IVRLP with trimethoprim-sulphadiazine cannot be recommended due to the low concentrations of synovial fluid over time and the frequent severe adverse effects causing pain and discomfort in treated horses. Thus, in cases of septic synovitis with bacteria sensitive to trimethoprim-sulphadiazine, other routes of administration should be considered.

Sign in / Sign up

Export Citation Format

Share Document