scholarly journals Hematological and Biochemical Reference Values in Healthy Captive Tigers (Panthera tigris)

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3440
Author(s):  
Daniela Proverbio ◽  
Roberta Perego ◽  
Luciana Baggiani ◽  
Giuliano Ravasio ◽  
Daniela Giambellini ◽  
...  
Keyword(s):  
Endangered Species ◽  
Blood Cells ◽  
White Blood Cells ◽  
Panthera Tigris ◽  
Free Living ◽  
Mean Cell Volume ◽  
Mean Values ◽  
Normal Ranges ◽  
The Mean ◽  
Tiger Population

The tiger (Panthera tigris) is an endangered species. The health of individuals is important and any data on hematological and biochemical blood values can provide valuable information; when combined with physical assessment. This data assists in both the diagnosis of disease and some conservation strategies. The behavior of wild tigers makes it is extremely difficult to obtain biological samples from free-living subjects, therefore, data collected from captive tigers is highly valuable. The aim of this study was to provide additional information for the values of hematological and serum biochemical parameters in healthy captive tigers. Blood samples were collected from 22 clinically healthy tigers (Panthera tigris). The following parameters were analyzed: glucose, urea, creatinine, alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein (TP) and red blood cells (RBCs), hemoglobin (Hb), hematocrit (Hct) and red cell indices; such as mean cell volume (MCV), mean cell Hb (MCH), mean cell Hb concentration (MCHC), platelet (PLT) and white blood cells (WBCs). The mean hematological values in our tiger population were not significantly different when compared with the same parameters in the previously studied tiger population. The mean values of RBCs and PLT were statistically significantly higher and the mean values of Hb, PCV, MCV, MCH, MCHC, and WBC were lower than the mean values obtained in previous studies on the Amur tiger. Further investigation of captive and free-living tigers is needed to identify the normal ranges for parameters in this endangered species.

scholarly journals Propiscin — A safe anaesthetic for pikeperch (Sander Lucioperca L.)

2016 ◽  
Vol 64 (4) ◽  
pp. 415-424 ◽  
Author(s):  
Maciej Rożyński ◽  
Elżbieta Ziomek ◽  
Krystyna Demska-Zakęś ◽  
Agata Kowalska ◽  
Zdzisław Zakęś
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Blood Parameters ◽  
Control Group ◽  
Sander Lucioperca ◽  
Mean Values ◽  
Recirculating Aquaculture ◽  
Calcium Magnesium ◽  
The Mean ◽  
The Impact

The aim of this work was to determine the impact of etomidate (Propiscin) dose (1 and 2 ml l−1) and exposure time (2 and 10 min) on the biochemical and haematological parameters of juvenile pikeperch (Sander lucioperca) [mean body length (Lc) 25.9 cm; body weight (W) 189.9 g] that were reared in a recirculating aquaculture system (RAS). Significant changes in the mean values of total protein, globulin, calcium, magnesium, and ammonia were noted in all groups immediately following exposure. The greatest changes in the haematological indicators were observed in groups subjected to 10-min exposure at both doses of the anaesthetic. The specimens from these groups had higher values for white blood cells (WBC), red blood cells (RBC), haemoglobin (HGB), haematocrit (HCT), and mean corpuscular volume (MCV). Statistically significant differences in these same parameters were also noted in the groups of fish exposed to the anaesthetic for 2 min at a dose of 2 ml l−1, but they were not as pronounced. Twenty-four h following exposure to etomidate, all blood parameters in the experimental groups were comparable to those of the control group. Etomidate can be recommended as a safe, effective anaesthetic for pikeperch.

scholarly journals ENVIRONMENTAL PESTICIDE RESIDUES AND HEALTH BIOMARKERS AMONG FARMERS FROM GREENHOUSES OF ERBIL CUCUMBER CROPS

2020 ◽  
Vol 51 (5) ◽  
pp. 1357-1366
Author(s):  
Othman & Kakey
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Total Serum ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Normal Ranges ◽  
Groundwater Samples ◽  
Control Serum ◽  
Function Test ◽  
Greenhouse Cucumber ◽  
The Mean

This study was aimed to determine the residual concentrations of pesticides used in greenhouse cucumber fields and their effects on the farmers that spray it. Sixteen active cucumber greenhouse fields with 96 people (75 sprayers and 21 volunteers as control) randomly had been selected. In all selected greenhouses, abamectin, thiamethoxam, pyridabin, and spirodiclofen pesticides were used, their cucumber with leaves and groundwater collected in December 2017, March, June, and September 2018 with workers blood samples, were immediately brought to the laboratory. Results showed the occurrence of residual pesticides in the plant and groundwater samples with a highest level in the plant samples, while decreased in water samples. That is in comparison with control, sprayer blood results showed that cholinesterase activity decreased significantly meanwhile, liver function enzymes: serum glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, alkaline phosphatase, direct and total serum bilirubin increased significantly. The kidney function test revealed that the mean serum urea concentration in the sprayers was considerably increased compared to the controls, while there was no significant change between the sprayers and the control serum creatinine. Slight variations in the declining number of red blood cells; however, white blood cells have significantly risen to their upper limit within normal ranges.

scholarly journals The prevalence of leukocyte abnormalities among Sudanese patients with sickle cell disease

2021 ◽  
Vol 9 (1) ◽  
pp. 262-267
Author(s):  
Tarig Osman Khalafallah Ahmed ◽  
Ekhlas Alrasheid Abu Elfadul ◽  
Ahmed A. Agab Eldour ◽  
Omer Ibrahim Abdallah Mohammed
Keyword(s):  
Sickle Cell Disease ◽  
Cell Volume ◽  
Sickle Cell ◽  
Blood Cells ◽  
White Blood Cells ◽  
Blood Film ◽  
Cell Disease ◽  
Mean Cell Volume ◽  
Blood Disorder ◽  
Age Range

Sickle cell disease (SCD) is an inherited blood disorder that affects red blood cells. The study was conducted in Elobied town during the period May 2011 to September 2011. The aim of this study is to detect the abnormalities of leucocytes among sickle cell anemic patients. 40 sickle cell anemic patients; age range between 8 months to 23 years. Blood sample was taken for all patients and the laboratory investigation were performed using automated estimation for: hemoglobin (Hb), Packed cell volume (PCV), red cell count (RBCs), mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell concentration (MCHC), and total white blood cells, comment on blood film using manual methods. The conclusion of this study there is increase in total white blood cells with shift to left in neutrophil precursor in sickle cell patients with complications ,the most immature cells are band form, myelocytes and metamyelocytes, and there also lymphocytosis and neutrophilia which has been increases in response to infections.

Interference of Plasma Fluorophores on the Red Blood Cell Zinc Protoporphyrin : Haemoglobin Ratio as Determined on a Haematofluorimeter

1989 ◽  
Vol 26 (4) ◽  
pp. 368-373 ◽  
Author(s):  
P C Bartels ◽  
P W Helleman ◽  
J B J Soons
Keyword(s):  
Blood Cells ◽  
Extraction Method ◽  
Isotonic Saline ◽  
Zinc Protoporphyrin ◽  
Reference Ranges ◽  
Reference Groups ◽  
Blood Samples ◽  
Mean Values ◽  
Good Relationship ◽  
The Mean

Direct measurement of the zinc protoporphyrin:haemoglobin ratio (ZPP: Hb ratio) in blood samples is performed by using a haematofluorimeter. Interference by non-specific fluorophores can be eliminated by removing the plasma and making the measurement on washed red blood cells (RBCs). After re-suspending RBCs in isotonic saline, haematofluorimeter readings for the ZPP: Hb ratios revealed higher stability in the course of time whereas a good relationship was found with results obtained by application of an extraction method. Separate reference ranges were established for adult male and female subjects. After washing, the mean values calculated for ZPP: Hb ratios of subjects belonging to the reference groups demonstrated a reduction of 0·04 μmol ZPP mol Hb, corresponding with approximately 30%. In the patients' group, application of washing resulted in a variable decrease of ZPP: Hb ratios.

scholarly journals Experimental infections of baboons (Papio spp.) and vervet monkeys (Cercopithecus aethiops) with Trichinella zimbabwensis and successful treatment with ivermectin

2008 ◽  
Vol 75 (2) ◽  
Author(s):  
S. Mukaratirwa ◽  
B. M. Dzoma ◽  
E. Matenga ◽  
S. D. Ruziwa ◽  
L. Sacchi ◽  
...  
Keyword(s):  
Packed Cell Volume ◽  
Post Infection ◽  
Blood Cells ◽  
White Blood Cells ◽  
Clinical Signs ◽  
Blood Parameters ◽  
Cercopithecus Aethiops ◽  
Vervet Monkeys ◽  
Muscular Pain ◽  
Normal Ranges

Experimental Trichinella zimbabwensis infections were established in three baboons (Papios p.)and four vervet monkeys (Cercopithecuase thiops) and the clinical-pathological manifestations assessed. The infected animals showed clinical signs ranging from fever, diarrhoea, periorbitaol edema and muscular pain in varying degrees. One baboon became blind due to the infection. Levels of creatinine phosphokinase and lactated ehydrogenase increased to reach a peak on Day 42 post-infection(pi)for both baboons and monkeys. Blood parameters such as packed cell volume, levels of red blood cells and white blood cells did not change significantly from the normal ranges except for the levels of eosinophils which peaked above the normal ranges at Day 28 and 56 pi in baboons and at Day 56 pi in monkeys.

scholarly journals The Quality Assessment of Stored Red Blood Cells Probed Using Atomic-Force Microscopy

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
I. M. Lamzin ◽  
R. M. Khayrullin
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Morphological Characteristics ◽  
High Increase ◽  
Mean Values ◽  
Force Microscopy ◽  
Atomic Force ◽  
The Mean ◽  
The Moment

At the moment the suitability of stored red blood cells (sRBC) for transfusion is checked by routine methods such as haemoglobin estimation and the level of haemolysis. These methods cannot characterize directly the quality of the membranes of sRBC. The aim of this work is to assess the quality of sRBC based on such criteria as the membrane’s stiffness and the size and the form of sRBC. Materials and Methods. We have investigated 5 series of dry cytosmears of the sRBC which had been kept in blood bank in a period from 1 to 35 days. After AFM imaging, in every specimen, 5 RBC were chosen at random; the diameter, the height, and the stiffness were measured on each of them. Results. The present study shows high increase of the mean values of YM and height of RBC after 35 days of storage and decrease of the mean values of their diameter. Conclusion. Statistically significant high increase of the mean values of YM indicates the decrease of the elasticity of the cells in the course of storing of the RBC. This parameter along with the morphological characteristics can be used as criterion for assessment of applicability of the sRBC for blood transfusion.

scholarly journals Uncomplicated falciparum malaria among schoolchildren in Bajil district of Hodeidah governorate, west of Yemen: association with anaemia and underweight

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Talal S. Alwajeeh ◽  
Rashad Abdul-Ghani ◽  
Amal F. Allam ◽  
Hoda F. Farag ◽  
Safia S. M. Khalil ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Falciparum Malaria ◽  
White Blood Cells ◽  
Significant Independent Predictor ◽  
Cross Sectional ◽  
Mean Values ◽  
Cell Counts ◽  
Normal Ranges ◽  
Mild Anaemia ◽  
Haematological Indices

Abstract Background Malaria, malnutrition and anaemia are major public health problems in Yemen, with Hodeidah being the most malaria-afflicted governorate. To address the lack of relevant studies, this study was conducted to determine the prevalence of Plasmodium falciparum and its relation to nutritional status and haematological indices among schoolchildren in Bajil district of Hodeidah governorate, west of Yemen. Methods A cross-sectional study was conducted among 400 schoolchildren selected randomly from four schools in Bajil district. Data about demographic characteristics, risk factors and anthropometric measurements of age, height and weight were collected. Duplicate thick and thin blood films were prepared, stained with Giemsa and examined microscopically for malaria parasites. The density of P. falciparum asexual stages was estimated on thick films. EDTA-blood samples were examined for the haematological indices of haemoglobin (Hb) and blood cell counts. Results Plasmodium falciparum was prevalent among 8.0% (32/400) of schoolchildren with a mean parasite density of 244.3 ± 299.3/µL of blood and most infections showing low-level parasitaemia, whereas Plasmodium vivax was detected in one child (0.25%). Residing near water collections was a significant independent predictor of falciparum malaria [adjusted odds ratio (AOR) = 2.6, 95.0% CI 1.20–5.72; p = 0.016] in schoolchildren. Mild anaemia was prevalent among more than half of P. falciparum-infected schoolchildren and significantly associated with falciparum malaria (AOR = 5.8, 95.0% CI 2.39–14.17; p < 0.001), with a mean Hb concentration of 10.7 ± 1.0 g/dL. Although the mean values of the total white blood cells, monocytes and platelets were significantly lower in infected than non-infected schoolchildren, they were within normal ranges. More than half of the children were malnourished, with stunting (39.3%) and underweight (36.0%) being the most prevalent forms of malnutrition; 6.3% of children were wasted. Underweight (AOR = 5.3, 95.0% CI 2.09–13.62; p < 0.001) but not stunting or wasting, was a significant predictor of falciparum malaria among schoolchildren. Conclusion Asymptomatic falciparum malaria is prevalent among schoolchildren in Bajil district of Hodeidah Governorate, with predominance of low parasitaemic infections and significant association with mild anaemia and underweight. Residence near water collection is a significant predictor of infection with falciparum malaria among schoolchildren. Further studies among children with severe malaria and those with high parasite densities are recommended.

Erythroplateletapheresis: A New Strategy in the Global Apheresis Program

1998 ◽  
Vol 21 (6_suppl) ◽  
pp. 17-19
Author(s):  
G. Menichella ◽  
M. Ciarli ◽  
R. Serafini ◽  
L. Pierelli ◽  
M. Vittori ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Plasma Volume ◽  
Blood Cells ◽  
Storage Time ◽  
Blood Donation ◽  
Mean Values ◽  
Packed Red Blood Cells ◽  
New Strategy ◽  
The Mean

Blood donation allowed by cell separators can offer higher performance and higher yield to guarantee better quality and pureness of collected products. New systems for the collection of platelet concentrate (PC) and packed red blood cells (PRBC) are currently available. The aim of our work was to test the possibility of preparing PC routinely from normal apheresis donors in a minimum amount of time while providing a second product. Over a 3-month period we performed 40 procedures using the Hemonetics MCS3P blood cell separator and the Dideco Excel. The mean values of platelet yield were 2.8 x 1011 (range 1.4-4.1) with the MCS3P and 3.49 x 1011 (range 2.9-3.9) with the Excel, in a plasma volume of 240 ml and 215 ml respectively; the PRBC units were added with SAG-Mannitol allowing a storage time of 42 days. Collection times were 71’ and 48’ respectively. Donor tolerance was analogous to phateletapheresis or plasmapheresis.

scholarly journals Evaluation of Baseline Hematologic Parameters of Steady State Sickle Cell Disease Patients at The Chantal Biya’s Foundation, Yaounde- Cameroon

2019 ◽  
Vol 7 (07) ◽  
Author(s):  
Bih Adelaide ◽  
Ngala Solange Mudih ◽  
Alima Yanda ◽  
Akaba Fergus Ambe ◽  
Jutcha Florent ◽  
...  
Keyword(s):  
Steady State ◽  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Reference Values ◽  
Blood Cells ◽  
Healthy Subjects ◽  
White Blood Cells ◽  
Great Decrease ◽  
Cell Disease ◽  
Mean Values

Introduction Sickle cell disease is a genetic abnormality involving the hemoglobin. Although it is primarily a red blood cell disorder, the white blood cells and platelets are also affected by the mutation. The consequence hemoglobin S causing polymerization of hemoglobin, results in hemolysis and anemia. This study aims to provide baseline hematologic parameters in steady state sickle cell disease patients compared with the reference values of normal healthy subjects used at the Chantal Biya Foundation (CBF), in order to monitor other sicklers in Cameroon. Methodology A comparative analysis of sickle cell hematologic parameters with control hematologic parameters of normal healthy subjects (reference values of healthy subjects used at the Chantal Biya Foundation) was carried out. Results A total of 62 sickle cell disease patients in steady state who complied with the selection criteria were recruited. Of the 62, 29 were females and 33 were males. The age range was from 1 year to 19 years and an average age of 6 ± 4.19 SD. Results from sickle cell patients showed an increase in white blood cells (WBCs), neutrophils and lymphocytes and a great decrease in the mean values of hematocrit Hct. as well as RBC indices, but no great or slight difference in the values of basophils compared with the reference values of normal healthy subjects in the CBF Yaoundé, 2015. Conclusion Sickle cell disease patients in steady state have lower values of red cells parameters, but higher values of white cells and platelets count when compared with the reference values of normal healthy subjects used at the Chantal Biya Foundation.

scholarly journals Uncomplicated falciparum malaria among schoolchildren in Bajil district of Hodeidah governorate, west of Yemen: Association with anaemia and underweight

2020 ◽  
Author(s):  
Talal S Alwajeeh ◽  
Rashad Abdul-Ghani ◽  
Amal F. Allam ◽  
Hoda F. Farag ◽  
Safia S.M. Khalil ◽  
...  
Keyword(s):  
Falciparum Malaria ◽  
White Blood Cells ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Mean Values ◽  
Cell Counts ◽  
Normal Ranges ◽  
Mild Anaemia ◽  
Hb Concentration ◽  
Haematological Indices

Abstract Background Malaria, malnutrition and anaemia are major public health problems in Yemen, with Hodeidah being the most malaria-afflicted governorate. To address the lack of relevant studies, this study was conducted to determine the prevalence of Plasmodium falciparum and its relation to nutritional status and haematological indices among schoolchildren in Bajil district of Hodeidah governorate, west of Yemen. Methods This cross-sectional study was conducted among 400 schoolchildren selected randomly from four schools in Bajil district. Data about demographic characteristics, risk factors and anthropometric measurements of age, height and weight were collected. Duplicate thick and thin blood films were prepared, stained with Giemsa and examined microscopically for malaria parasites. The density of P. falciparum asexual stages was estimated on thick films. EDTA-blood samples were examined for the haematological indices of haemoglobin (Hb) and blood cell counts. Results P. falciparum was prevalent among 8.0% (32/400) of schoolchildren with a mean parasite density of 244.3 \(\pm\)299.3/µl of blood and most infections showing low-level parasitaemia, whereas Plasmodium vivax was detected in one child (0.25%). Residing near water collections was a significant predictor of falciparum malaria (OR = 2.4, 95% CI = 1.14–5.05; p = 0.019) in schoolchildren. Mild anaemia was prevalent among more than half of P. falciparum-infected schoolchildren and significantly associated with falciparum malaria, with a mean Hb concentration of 10.7 \(\pm\)1.0 g/dL. Although the mean values of the total white blood cells, monocytes and platelets were significantly lower in infected than non-infected schoolchildren, they were within normal ranges. More than half of the children were malnourished, with stunting (39.3%) and underweight (36.0%) being the most prevalent forms of malnutrition; 6.3% of children were wasted. Underweight (OR = 3.3, 95% CI = 1.55–6.93; p = 0.001), but not stunting or wasting, was a significant predictor of falciparum malaria among schoolchildren. Conclusion Asymptomatic falciparum malaria is prevalent among schoolchildren in Bajil district of Hodeidah Governorate, with predominance of low parasitaemic infections and significant association with mild anaemia and underweight. Residence near water collection is a significant predictor of infection with falciparum malaria among schoolchildren. Further studies among children with severe malaria and those with high parasite densities are recommended.

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