Bta-miR-24-3p Controls the Myogenic Differentiation and Proliferation of Fetal, Bovine, Skeletal Muscle-Derived Progenitor Cells by Targeting ACVR1B

MicroRNAs modulate a variety of cellular events, including skeletal muscle development, but the molecular basis of their functions in fetal bovine skeletal muscle development is poorly understood. In this study, we report that bta-miR-24-3p promotes the myogenic differentiation of fetal bovine PDGFRα- progenitor cells. The expression of bta-miR-24-3p increased during myogenic differentiation. Overexpression of bta-miR-24-3p significantly promoted myogenic differentiation, but inhibited proliferation. A dual-luciferase assay identified ACVR1B as a direct target of bta-miR-24-3p. Similarly, knocking down ACVR1B by RNA interference also significantly inhibited proliferation and promoted the differentiation of bovine PDGFRα- progenitor cells. Thus, our study provides a mechanism in which bta-miR-24-3p regulates myogenesis by inhibiting ACVR1B expression.

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bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting MDFIC Gene

Genes ◽

10.3390/genes11101232 ◽

2020 ◽

Vol 11 (10) ◽

pp. 1232

Author(s):

Xin Hu ◽

Yishen Xing ◽

Ling Ren ◽

Yahui Wang ◽

Qian Li ◽

...

Keyword(s):

Skeletal Muscle ◽

Transcription Factor ◽

Progenitor Cells ◽

Myogenic Differentiation ◽

Luciferase Reporter ◽

Down Regulation ◽

Promising Target ◽

Reporter Assays ◽

Fetal Bovine ◽

Bovine Skeletal Muscle

miR-23a, a member of the miR-23a/24-2/27a cluster, has been demonstrated to play pivotal roles in many cellular activities. However, the mechanisms of how bta-miR-23a controls the myogenic differentiation (MD) of PDGFRα− bovine progenitor cells (bPCs) remain poorly understood. In the present work, bta-miR-23a expression was increased during the MD of PDGFRα− bPCs. Moreover, bta-miR-23a overexpression significantly promoted the MD of PDGFRα− bPCs. Luciferase reporter assays showed that the 3’-UTR region of MDFIC (MyoD family inhibitor domain containing) could be a promising target of bta-miR-23a, which resulted in its post-transcriptional down-regulation. Additionally, the knockdown of MDFIC by siRNA facilitated the MD of PDGFRα− bPCs, while the overexpression of MDFIC inhibited the activating effect of bta-miR-23a during MD. Of note, MDFIC might function through the interaction between MyoG transcription factor and MEF2C promoter. This study reveals that bta-miR-23a can promote the MD of PDGFRα− bPCs through post-transcriptional downregulation of MDFIC.

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Shift from slow- to fast-twitch muscle fibres in skeletal muscle of newborn heterozygous and homozygous myostatin-knockout piglets

Reproduction Fertility and Development ◽

10.1071/rd19103 ◽

2019 ◽

Vol 31 (10) ◽

pp. 1628 ◽

Cited By ~ 2

Author(s):

Mei-Fu Xuan ◽

Zhao-Bo Luo ◽

Jun-Xia Wang ◽

Qing Guo ◽

Sheng-Zhong Han ◽

...

Keyword(s):

Skeletal Muscle ◽

Protein Expression ◽

Muscle Development ◽

Transforming Growth Factor ◽

Myogenic Differentiation ◽

Muscle Fibres ◽

Skeletal Muscle Development ◽

Cross Sectional ◽

Gene And Protein Expression ◽

Fast Twitch

Myostatin (MSTN) is a member of the transforming growth factor-β superfamily that negatively regulates skeletal muscle development. A lack of MSTN induces muscle hypertrophy and increases formation of fast-twitch (Type II) muscle fibres. This study investigated muscle development in newborn heterozygous (MSTN+/−) and homozygous (MSTN−/−) MSTN-knockout piglets. Detailed morphological and gene and protein expression analyses were performed of the biceps femoris, semitendinosus and diaphragm of MSTN+/−, MSTN−/− and wild-type (WT) piglets. Haematoxylin–eosin staining revealed that the cross-sectional area of muscle fibres was significantly larger in MSTN-knockout than WT piglets. ATPase staining demonstrated that the percentage of Type IIb and IIa muscle fibres was significantly higher in MSTN−/− and MSTN+/− piglets respectively than in WT piglets. Western blotting showed that protein expression of myosin heavy chain-I was reduced in muscles of MSTN-knockout piglets. Quantitative reverse transcription–polymerase chain reaction revealed that, compared with WT piglets, myogenic differentiation factor (MyoD) mRNA expression in muscles was 1.3- to 2-fold higher in MSTN+/− piglets and 1.8- to 3.5-fold higher MSTN−/− piglets (P<0.05 and P<0.01 respectively). However, expression of myocyte enhancer factor 2C (MEF2C) mRNA in muscles was significantly lower in MSTN+/− than WT piglets (P<0.05). MSTN plays an important role in skeletal muscle development and regulates muscle fibre type by modulating the gene expression of MyoD and MEF2C in newborn piglets.

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Overexpression of the Rybp Gene Inhibits Differentiation of Bovine Myoblasts into Myotubes

International Journal of Molecular Sciences ◽

10.3390/ijms19072082 ◽

2018 ◽

Vol 19 (7) ◽

pp. 2082

Author(s):

Xiaotong Su ◽

Yanfang Zhao ◽

Yaning Wang ◽

Le Zhang ◽

Linsen Zan ◽

...

Keyword(s):

Skeletal Muscle ◽

Muscle Development ◽

Molecular Breeding ◽

Theoretical Foundation ◽

Negative Control ◽

Myoblast Differentiation ◽

Rna Seq ◽

Skeletal Muscle Development ◽

Morphological Differences ◽

Bovine Skeletal Muscle

RING1 and YY1 binding protein (Rybp) genes inhibit myogenesis in mice, but there are no reports on the effects of these genes in cattle. The aim of this study is to investigate the roles of the Rybp gene on bovine skeletal muscle development and myoblast differentiation. In the present study, the Rybp gene was overexpressed in bovine myoblasts via adenovirus. RNA-seq was performed to screen differentially expressed genes (DEGs). The results showed that overexpressing the Rybp gene inhibits the formation of myotubes. The morphological differences in myoblasts began on the second day and were very significant 6 days after adenovirus induction. A total of 1311 (707 upregulated and 604 downregulated) DEGs were screened using RNA-seq between myoblasts with added negative control adenoviruses (AD-NC) and Rybp adenoviruses (AD-Rybp) after 6 days of induction. Gene ontology (GO) and KEGG analysis revealed that the downregulated DEGs were mainly involved in biological functions related to muscle, and, of the 32 pathways, those associated with muscle development were significantly enriched for the identified DEGs. This study can not only provide a theoretical basis for the regulation of skeletal muscle development in cattle by exploring the roles of the Rybp gene in myoblast differentiation, but it can also lay a theoretical foundation for molecular breeding of beef cattle.

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Profiling and Functional Analysis of Long Noncoding RNAs and mRNAs during Porcine Skeletal Muscle Development

International Journal of Molecular Sciences ◽

10.3390/ijms22020503 ◽

2021 ◽

Vol 22 (2) ◽

pp. 503

Author(s):

Ya Tan ◽

Mailin Gan ◽

Linyuan Shen ◽

Liang Li ◽

Yuan Fan ◽

...

Keyword(s):

Gene Expression ◽

Skeletal Muscle ◽

Inflection Point ◽

Muscle Development ◽

Noncoding Rnas ◽

Long Noncoding Rnas ◽

Functional Enrichment ◽

Differentially Expressed ◽

Luciferase Assay ◽

Skeletal Muscle Development

Gene transcripts or mRNAs and long noncoding RNAs (lncRNAs) are differentially expressed during porcine skeletal muscle development. However, only a few studies have been conducted on skeletal muscle transcriptome in pigs based on timepoints according to the growth curve for porcine. Here, we investigated gene expression in Qingyu pigs at three different growth stages: the inflection point with the maximum growth rate (MGI), the inflection point of the gradually increasing stage to the rapidly increasing stage (GRI), and the inflection point of the rapidly increasing stage to the slowly increasing stage (RSI). Subsequently, we explored gene expression profiles during muscle development at the MGI, GRI and RSI stages by Ribo-Zero RNA sequencing. Qingyu pigs reached the MGI, GRI and RSI stages at 156.40, 23.82 and 288.97 days of age with 51.73, 3.14 and 107.03 kg body weight, respectively. A total of 14,530 mRNAs and 11,970 lncRNAs were identified at the three stages, and 645, 323 differentially expressed genes (DEGs) and 696, 760 differentially expressed lncRNAs (DELs) were identified in the GRI vs. MGI, and RSI vs. MGI, comparisons. Functional enrichment analysis revealed that genes involved in immune system development and energy metabolism (mainly relate to amino acid, carbohydrate and lipid) were enriched at the GRI and MGI stages, respectively, whereas genes involved in lipid metabolism were enriched at the RSI stage. We further characterized G1430, an abundant lncRNA. The full-length sequence (316 nt) of lncRNA G1430 was determined by rapid amplification of cDNA ends (RACE). Subcellular distribution analysis by quantitative real-time PCR (qRT-PCR) revealed that G1430 is a cytoplasmic lncRNA. Binding site prediction and dual luciferase assay showed that lncRNA G1430 directly binds to microRNA 133a (miR-133a). Our findings provide the basis for further investigation of the regulatory mechanisms and molecular genetics of muscle development in pigs.

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The SMYD3 methyltransferase promotes myogenesis by activating the myogenin regulatory network

Scientific Reports ◽

10.1038/s41598-019-53577-5 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 4

Author(s):

Roberta Codato ◽

Martine Perichon ◽

Arnaud Divol ◽

Ella Fung ◽

Athanassia Sotiropoulos ◽

...

Keyword(s):

Skeletal Muscle ◽

Muscle Development ◽

Myogenic Differentiation ◽

Muscle Differentiation ◽

Skeletal Muscle Differentiation ◽

Skeletal Muscle Development ◽

Genome Wide ◽

Coordinated Expression

AbstractThe coordinated expression of myogenic regulatory factors, including MyoD and myogenin, orchestrates the steps of skeletal muscle development, from myoblast proliferation and cell-cycle exit, to myoblast fusion and myotubes maturation. Yet, it remains unclear how key transcription factors and epigenetic enzymes cooperate to guide myogenic differentiation. Proteins of the SMYD (SET and MYND domain-containing) methyltransferase family participate in cardiac and skeletal myogenesis during development in zebrafish, Drosophila and mice. Here, we show that the mammalian SMYD3 methyltransferase coordinates skeletal muscle differentiation in vitro. Overexpression of SMYD3 in myoblasts promoted muscle differentiation and myoblasts fusion. Conversely, silencing of endogenous SMYD3 or its pharmacological inhibition impaired muscle differentiation. Genome-wide transcriptomic analysis of murine myoblasts, with silenced or overexpressed SMYD3, revealed that SMYD3 impacts skeletal muscle differentiation by targeting the key muscle regulatory factor myogenin. The role of SMYD3 in the regulation of skeletal muscle differentiation and myotube formation, partially via the myogenin transcriptional network, highlights the importance of methyltransferases in mammalian myogenesis.

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Stem and Progenitor Cells in Skeletal Muscle Development, Maintenance, and Therapy

Molecular Therapy ◽

10.1038/mt.sj.6300145 ◽

2007 ◽

Vol 15 (5) ◽

pp. 867-877 ◽

Cited By ~ 406

Author(s):

Bruno Péault ◽

Michael Rudnicki ◽

Yvan Torrente ◽

Giulio Cossu ◽

Jacques P Tremblay ◽

...

Keyword(s):

Skeletal Muscle ◽

Progenitor Cells ◽

Muscle Development ◽

Skeletal Muscle Development ◽

Stem And Progenitor Cells

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Profiling and Functional Analysis of Long Noncoding RNAs and mRNAs during Porcine Skeletal Muscle Development

10.21203/rs.3.rs-104074/v1 ◽

2020 ◽

Author(s):

Ya Tan ◽

Mailin Gan ◽

Linyuan Shen ◽

Liang Li ◽

Yuan Fan ◽

...

Keyword(s):

Gene Expression ◽

Skeletal Muscle ◽

Inflection Point ◽

Muscle Development ◽

Noncoding Rnas ◽

Long Noncoding Rnas ◽

Functional Enrichment ◽

Differentially Expressed ◽

Luciferase Assay ◽

Skeletal Muscle Development

Abstract BackgroundGene transcripts or mRNAs and long noncoding RNAs (lncRNAs) are differentially expressed during porcine skeletal muscle development. However, only a few studies have been conducted on skeletal muscle transcriptome in pigs based on timepoints according to the growth curve for porcine. Here, we investigated gene expression in Qingyu pigs at three different growth stages: of the inflection point with the maximum growth rate (MGI), inflection point of gradual increase stage to rapid increasing stage (GRI) and inflection point of rapid increasing stage to slowly increasing stage (RSI). Subsequently, we explored gene expression profiles during muscle development at the MGI, GRI and RSI stages by Ribo-Zero RNA sequencing. ResultsQingyu pigs reached the MGI, GRI and RSI stages at 156.40, 23.82 and 288.97 days of age with 51.73, 3.14 and 107.03 kg body weight, respectively. A total of 14,530 mRNAs and 11,970 lncRNAs were identified at the three stages, and 645, 323 differentially expressed genes (DEGs) and 696, 760 differentially expressed lncRNAs (DELs) were identified in the GRI vs. MGI, RSI vs. MGI comparisons. Functional enrichment analysis revealed that genes involved in immune system development and energy metabolism (mainly relate to amino acid, carbohydrate and lipid) were enriched at the GRI and MGI stages, respectively, whereas genes involved in energy and lipid metabolism were enriched at the RSI stage. We further characterized G1430, an abundant lncRNA. The full-length sequence (316 nt) of lncRNA G1430 was determined by rapid amplification of cDNA ends (RACE). Subcellular distribution analysis by quantitative real-time PCR (qRT-PCR) revealed that G1430 is a cytoplasmic lncRNA. Binding site prediction and dual luciferase assay showed that lncRNA G1430 directly binds to microRNA 133a (miR-133a). ConclusionThese findings indicate lncRNAs and a certain lncRNA G1430 involved in the regulatory mechanism during pig muscle development. Our findings provide the basis for further investigation of the regulatory mechanisms and molecular genetics of muscle development in pigs.

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bta-miR-23a involves in adipogenesis of progenitor cells derived from fetal bovine skeletal muscle

Scientific Reports ◽

10.1038/srep43716 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 33

Author(s):

Long Guan ◽

Xin Hu ◽

Li Liu ◽

Yishen Xing ◽

Zhengkui Zhou ◽

...

Keyword(s):

Skeletal Muscle ◽

Progenitor Cells ◽

Fetal Bovine ◽

Bovine Skeletal Muscle

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MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway

International Journal of Molecular Sciences ◽

10.3390/ijms21093274 ◽

2020 ◽

Vol 21 (9) ◽

pp. 3274

Author(s):

Huadong Yin ◽

Haorong He ◽

Xiaoxu Shen ◽

Shuyue Tang ◽

Jing Zhao ◽

...

Keyword(s):

Skeletal Muscle ◽

Signaling Pathway ◽

Muscle Development ◽

Transforming Growth Factor ◽

Skeletal Muscle Development ◽

Developmental Anatomy ◽

Smad Signaling ◽

Smad Signaling Pathway ◽

Differentiation And Proliferation

MicroRNAs (miRNAs) are evolutionarily conserved, small noncoding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. Chicken is an optimal model to study skeletal muscle formation because its developmental anatomy is similar to that of mammals. In this study, we identified potential miRNAs in the breast muscle of broilers and layers at embryonic day 10 (E10), E13, E16, and E19. We detected 1836 miRNAs, 233 of which were differentially expressed between broilers and layers. In particular, miRNA-200a-3p was significantly more highly expressed in broilers than layers at three time points. In vitro experiments showed that miR-200a-3p accelerated differentiation and proliferation of chicken skeletal muscle satellite cells (SMSCs) and inhibited SMSCs apoptosis. The transforming growth factor 2 (TGF-β2) was identified as a target gene of miR-200a-3p, and which turned out to inhibit differentiation and proliferation, and promote apoptosis of SMSCs. Exogenous TGF-β2 increased the abundances of phosphorylated SMAD2 and SMAD3 proteins, and a miR-200a-3p mimic weakened this effect. The TGF-β2 inhibitor treatment reduced the promotional and inhibitory effects of miR-200a-3p on SMSC differentiation and apoptosis, respectively. Our results indicate that miRNAs are abundantly expressed during embryonic skeletal muscle development, and that miR-200a-3p promotes SMSC development by targeting TGF-β2 and regulating the TGF-β2/SMAD signaling pathway.

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FHL2 Regulates Proliferation Differentiation and Autophagy of Bovine Skeletal Muscle Satellite Cells Through Wnt/β-catenin Signaling Pathway

10.21203/rs.3.rs-1180493/v1 ◽

2022 ◽

Author(s):

Yun Zhu ◽

Peng Li ◽

Xingang Dan ◽

Xingang Kang ◽

Yun Ma ◽

...

Keyword(s):

Skeletal Muscle ◽

Signaling Pathway ◽

Satellite Cells ◽

Muscle Development ◽

Wnt Signaling Pathway ◽

Skeletal Muscle Development ◽

Muscle Satellite Cells ◽

Skeletal Muscle Satellite Cells ◽

Proliferation And Differentiation ◽

Bovine Skeletal Muscle

Abstract The mechanism of physiological regulation of bovine skeletal muscle development is a complex process, and FHL2 has been studied in association with β-catenin activity and has previously been reported to play a role in skeletal muscle.However the mechanism of FHL2 action in regulating skeletal muscle development in bovine skeletal myosatellite is unclear. Here, we report that FHL2 can both promote the proliferation and differentiation of bovine myosatellite cells through the wnt signaling pathway and bovine skeletal muscle satellite cells through cellular autophagy. The results of western blotting, rt-qPCT, cell transfection assay showed that FHL2 gene expression was enhanced during the proliferation of skeletal muscle satellite cells, and FHL2 knockdown inhibited the proliferation and differentiation of bovine satellite cells and promoted the atrophy of myotubes. Furthermore, immunoprecipitation assays yielded that FHL2 knockdown decreased β-catenin activity in BMSCs and activated β-catenin-mediated wnt signaling pathway in combination with DVL-2, and that FHL2 knockdown induced autophagy in bovine satellite cells. Therefore, the FHL2 gene is a key gene in the regulation of bovine satellite cells.

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