scholarly journals Comparison of Faecal versus Rumen Inocula for the Estimation of NDF Digestibility

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 928 ◽  
Author(s):  
Maria Chiaravalli ◽  
Luca Rapetti ◽  
Andrea Rota Graziosi ◽  
Gianluca Galassi ◽  
Gianni Matteo Crovetto ◽  
...  
Keyword(s):  
Incubation Time ◽  
Rumen Fluid ◽  
Maize Silage ◽  
Ruminal Fluid ◽  
Feed Ingredients ◽  
Neutral Detergent Fibre ◽  
Lactating Cows ◽  
The Mean

Cow faeces have been investigated as alternative inoculum to replace rumen fluid to determine neutral detergent fibre (NDF) digestibility (NDFD). Aims of this study were to estimate: (1) the NDFD (48 h) of feed ingredients using a rumen inoculum in comparison with faecal inocula from cows fed diets with different forage basis; (2) the undigestible NDF (uNDF) at 240 and 360 h with ruminal fluid and faecal inocula from lactating cows fed two different diets. At 48 h incubation, the NDFD was affected both by feed and type of inoculum (p < 0.01) and by their interaction (p = 0.03). Overall, the mean NDFD was higher for rumen inoculum than for faecal inocula (585 vs. 389 g/kg NDF, p < 0.05), and faecal inoculum obtained from cows fed hay-based diets gave lower NDFD than those from cows fed maize silage (367 vs. 440 g/kg, p < 0.05). At long incubation times, the average uNDF was affected by substrate, inoculum and incubation time (p < 0.01), but not by their interactions. For each inoculum, significantly lower values were obtained at 360 than at 240 h. Regressions between uNDF with rumen and with the tested faecal inocula resulted in r2 ≥ 0.98. Despite the differences at 48 h, the uNDF showed that faecal inoculum could replace rumen fluid at longer incubation times.

Alterations in Ruminal Fluid and Serum Biochemical Constituents in Goats Affected with Ruminal Acidosis

2018 ◽  
Vol 13 (03) ◽  
Author(s):  
P. R. Chavelikar ◽  
G. and Neha Rao C. Mandali ◽  
Neha Rao
Keyword(s):  
Rumen Fluid ◽  
Clinical Signs ◽  
Small Ruminants ◽  
Ruminal Fluid ◽  
Mean Values ◽  
Biochemical Constituents ◽  
Ruminal Acidosis ◽  
Methylene Blue Reduction ◽  
Serum Biochemical ◽  
The Mean

Ruminal acidosis is an important clinical emergency in small ruminants. In this study, eight healthy farm goats and 24 goats presented at TVCC of the college of Veterinary Sciences and A.H., Anand with clinical signs of ruminal acidosis and having rumen liquor pH below 6 were examined for alterations in the ruminal fluid and serum biochemical parameters. Among various rumen fluid parameters evaluated, the mean values of rumen fluid pH decreased significantly (4.71±0.11 vs. 6.90±0.10), while sediment activity time (46.67±1.20 vs. 24.50±0.78 min) and methylene blue reduction time (29.50±0.73 vs. 10.03±0.27 min) increased significantly in acidotic goats. The normal greenish, aromatic viscous color, odour and consistency of rumen fluid of healthy goats also changed to milky grey/creamy, sour/pungent watery in acidotic goats. The rumen protozoal activity decreased to nil in acidotic goats as compared to the healthy goats. Among various serum biochemical constituents, the mean values of glucose (92.43±1.37 vs. 74.13±1.83 mg/dl), BUN (26.49±0.47 vs. 22.63±1.19 mg/dl), serum creatinine (01.01±0.02 vs. 00.83±0.02 mg/dl) and albumin (03.22±0.03 vs. 03.05±0.05 g/dl), ALT (56.75±1.55 vs. 27.88±1.14 IU/L) and AST (93.25±1.82 vs. 54.00±1.75 IU/L), increased significantly, while there was significant decrease in serum calcium (09.09±0.14 vs. 10.29±0.08 mg/dl) in acidotic goats. The mean values of alkaline phosphatase (IU/L) in acidotic goats increased non-significantly from the base values of healthy goats.

scholarly journals Determination of buffering capacity of selected fermented feedstuffs and the effect of dietary acid-base status on ruminal fluid pH

2011 ◽  
Vol 80 (3) ◽  
pp. 269-273 ◽  
Author(s):  
Lukáš Bujňák ◽  
Iveta Maskaľová ◽  
Vladimír Vajda
Keyword(s):  
Dairy Cows ◽  
Rumen Fluid ◽  
Buffering Capacity ◽  
Acid Base ◽  
Ruminal Fluid ◽  
Ruminal Ph ◽  
Lactating Cows ◽  
Buffer Value ◽  
Dietary Acid ◽  
Acid Base Status

The aim of this study was to evaluate the buffering capacity of some fermented feedstuffs and the effect of dietary acid-base status on ruminal fluid pH. The first experiment was performed with different types (n = 24) of wet and dry fermented feedstuffs to determine the buffering capacity and buffer value index. The buffer value index of wet corn silages was more negative than for dry corn silages account on acid loss during drying. In the second experiment, the effects of different concentrations of lactic acid on base-buffering capacity, buffer value index and indicators of fermentation process were studied in two groups of corn silages (n = 21). Two groups of analyzed values were compared using unpaired t-test. Differences among the groups in base-buffering capacity (P < 0.001) and buffer value index (P < 0.01) were significant. In the third experiment, rumen fluid pH was measured using 8 total mixed rations on 48 lactating dairy cows. In the present study no significant (P > 0.05) correlation was found between ruminal pH and dietary buffer value index. These results confirmed that dietary acid-base status alone is not adequate as a predictor of the need for buffers in the diet of lactating cows. It is the first report about using of buffering capacity determination methods for evaluation of feedstuffs and dietary acid-base status in dairy cows.

scholarly journals Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jessica Moraes Malheiros ◽  
Banny Silva Barbosa Correia ◽  
Caroline Ceribeli ◽  
Daniel Rodrigues Cardoso ◽  
Luiz Alberto Colnago ◽  
...  
Keyword(s):  
Ketone Bodies ◽  
Rumen Fluid ◽  
Bos Indicus ◽  
Metabolite Profile ◽  
Polar Compounds ◽  
Metabolome Analysis ◽  
Ruminal Fluid ◽  
Polar Metabolites ◽  
Close Relationship ◽  
Synthesis And Degradation

AbstractWe conducted a study to identify the fecal metabolite profile and its proximity to the ruminal metabolism of Nelore steers based on an untargeted metabolomic approach. Twenty-six Nelore were feedlot with same diet during 105 d. Feces and rumen fluid were collected before and at slaughter, respectively. The metabolomics analysis indicated 49 common polar metabolites in the rumen and feces. Acetate, propionate, and butyrate were the most abundant polar metabolites in both bio-samples. The rumen presented significantly higher concentrations of the polar compounds when compared to feces (P < 0.05); even though, fecal metabolites presented an accentuated representability of the ruminal fluid metabolites. All fatty acids present in the ruminal fluid were also observed in the feces, except for C20:2n6 and C20:4n6. The identified metabolites offer information on the main metabolic pathways (higher impact factor and P < 0.05), as synthesis and degradation of ketone bodies; the alanine, aspartate and glutamate metabolisms, the glycine, serine; and threonine metabolism and the pyruvate metabolism. The findings reported herein on the close relationship between the ruminal fluid and feces metabolic profiles may offer new metabolic information, in addition to facilitating the sampling for metabolism investigation in animal production and health routines.

PRELIMINARY EVALUATION OF THE ADDITION OF GLUCOGENIC MATERIALS TO THE RATIONS OF LACTATING COWS

1971 ◽  
Vol 51 (3) ◽  
pp. 721-727 ◽  
Author(s):  
L. J. FISHER ◽  
J. D. ERFLE ◽  
F. D. SAUER
Keyword(s):  
Fatty Acids ◽  
Propylene Glycol ◽  
Milk Fat ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Preliminary Evaluation ◽  
Early Lactation ◽  
Lactating Cows ◽  
Ad Libitum ◽  
Beta Hydroxybutyrate

Glutamate, succinate, propylene glycol, or glycerol were added to a basic concentrate at 3.3% of air-dry feed. Each concentrate was fed both ad libitum and in restricted amounts to four cows in early lactation. Dietary intake, milk yield and composition, molar proportions of rumen volatile fatty acids and blood glucose, ketones and plasma free fatty acids were used as criteria of effect of these supplements. Propylene glycol in the diet resulted in a lower intake of concentrate compared with glycerol (11.44 versus 14.30 kg/day) and significantly decreased (P < 0.05) rumen butyrate and plasma beta-hydroxybutyrate. Glutamate supplementation prevented the fall in milk fat content which occurred when the other three supplemented concentrates were fed ad libitum, and this effect may have been related to the constancy in the ratio of acetate to propionate in the rumen fluid.

Inter-laboratory variation in feedstuff evaluation of two contrasting maize silage samples

1996 ◽  
Vol 1996 ◽  
pp. 232-232
Author(s):  
D.E. Beever ◽  
S.B. Cammell ◽  
S. Edmonds
Keyword(s):  
Analytical Techniques ◽  
Maize Silage ◽  
Advisory Services ◽  
Lactating Cows ◽  
Feeding Value ◽  
Apparent Variation ◽  
Metabolisable Energy ◽  
The Uk

The importance of feedstuff evaluation as part of ration formulation is recognised by UK farmers, with the number of silage samples analysed increasing annually. These can be undertaken by the UK advisory services or feed companies, with several commercial laboratories offering similar services. However, as discussed by Beever [1993], there is concern over the authenticity of some estimates of feeding value, in relation to the analytical techniques used, the lack of agreed standardised procedures, and the apparent variation in results which exists between laboratories. This study examined the extent of this variation when 2 maize silage samples were independently analysed by 9 different laboratories, and compared laboratory based estimates of metabolisable energy [ME] contents with those derived by feeding the same diets to lactating cows.

scholarly journals Quality Traits of Longissimus thoracis and lumborum and Gluteus medium Muscles from Cull Dairy Holstein-Friesian Cows

2015 ◽  
Vol 4 (6) ◽  
pp. 82
Author(s):  
Carlos Santos ◽  
Carlos Moniz ◽  
Cristina Roseiro ◽  
Vera Medeiros ◽  
Isabel Afonso ◽  
...  
Keyword(s):  
Aging Time ◽  
Gluteus Medius ◽  
Meat Tenderness ◽  
Mean Values ◽  
Lactating Cows ◽  
Holstein Friesian ◽  
The Mean ◽  
Sensorial Attributes ◽  
Main Effects ◽  
Friesian Cows

<em>Longissimus thoracis</em> and <em>lumborum</em> (LTL) and <em>Gluteus medius</em> (Gm) muscles of culled dairy cows, differing in production status (Ps) at slaughter and carcass weight were assessed for intramuscular fat and myoglobin, color, shear force (SF) and sensorial characteristics, after being aged for 2, 7, 14, 28 and 42 days. Meat from dried-off cows was lighter, redder and had higher yellowness and chroma (<em>P </em>&lt; 0.05) than lactating cows. Meat of finished fed cows improved in color in relation to counterparts directly slaughtered, but not significantly. Color parameters increased with aging time and the mean values of LTL and Gm significantly differed mostly among lactating cows. SF of meat was affected by aging time, muscle type, Ps and by their respective interactions (<em>P</em> &lt; 0.001), with a greater impact in LTL muscle. Meat from LTL muscle of dried-off cows had lower SF (47.30 N) than that of lactating cows (65.61 N) (<em>P</em> &lt; 0.05), but such differences were not significant for Gm muscle. Meat tenderness of finished fed cows was higher than counterparts not finished (<em>P</em> &gt; 0.05). Main effects and the results from their interactions were significant for all sensorial attributes (<em>P</em> &lt; 0.001).

scholarly journals 372 Effect of rumen fluid collection methodology on extent and variance of in vitro dry matter disappearance and interaction with substrate and incubation time

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 155-156
Author(s):  
Emma L Rients ◽  
Sara M Tondini ◽  
Daniel W Shike ◽  
Joshua C McCann
Keyword(s):  
Incubation Time ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Fluid Collection ◽  
Stomach Tube ◽  
Time Points ◽  
Substrate Interaction ◽  
Time Interaction ◽  
Tube Suction

Abstract The objective was to evaluate the interaction of rumen fluid collection methodology, substrate, and incubation time on the extent and variance of in vitro dry matter disappearance (IVDMD). A 4×2×2 factorial design was used to test the interaction between rumen fluid collection methodology, substrates (hay and silage), and incubation time-points (24 and 48 h). Four methods of rumen fluid collection were evaluated: stomach tube (TUBE), suction strainer through rumen cannula (STRN), ruminal contents collected through cannula and squeezed through cheesecloth (SQZ), and ruminal contents blended for 1 minute and squeezed through cheesecloth (BSQZ). Four replications of each treatment were used and repeated in three periods. A methodology by substrate interaction (P < 0.01) was observed for pH; hay had a greater pH than silage and the magnitude of this difference was greatest for STRN. A tendency for a methodology by substrate interaction (P = 0.06) was observed for ammonia. For TUBE ammonia was greater (P < 0.01) with hay while substrate did not affect ammonia for BSQZ, SQZ and STRN. A methodology by time interaction was observed (P < 0.01) for ammonia; TUBE was least at 24 h, but TUBE and BSQZ were lesser (P ≤ 0.01) than STRN and SQZ at 48 h. A tendency for a methodology by substrate interaction (P = 0.08) was observed in IVDMD; silage had greater IVDMD than hay and the magnitude of this difference was greatest for TUBE. Collection methodology affected (P < 0.01) IVDMD. The greatest IVDMD was observed for STRN (64.7%), with BSQZ (59.1%) and SQZ (57.7%) being intermediate and TUBE (54.3%) being the least. A tendency for a methodology by substrate interaction (P = 0.11) was observed for variation in IVDMD; silage had greater variation than hay for TUBE. Rumen fluid collection methodology affects IVDMD and may depend on substrate.

Impact of a tannin extract on digestibility, ruminal fermentation and duodenal flow of amino acids in steers fed maize silage and concentrate containing soybean meal or canola meal as protein source

2015 ◽  
Vol 153 (5) ◽  
pp. 943-953 ◽  
Author(s):  
S. C. ÁVILA ◽  
G. V. KOZLOSKI ◽  
T. ORLANDI ◽  
M. P. MEZZOMO ◽  
S. STEFANELLO
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Soybean Meal ◽  
Rumen Fluid ◽  
Protein Source ◽  
Canola Meal ◽  
Ruminal Fermentation ◽  
Tannin Extract ◽  
Maize Silage ◽  
Microbial N

SUMMARYFour Holstein steers (297 ± 56 kg of body weight (BW)) fitted with duodenal cannula and rumen catheter, were housed in metabolism cages and used in a 4 × 4 Latin Square trial to evaluate the effect of both protein source andAcacia mearnsiitannin extract on digestibility, ruminal fermentation, rumen microbial protein synthesis, N utilization and on duodenal flow of individual amino acids. The diet was offered at restricted amount of 25 g of dry matter (DM)/kg BW and consisted of maize silage plus concentrate, in a proportion of 0·7:0·3 (DM basis) respectively. Concentrate was formulated with either soybean meal or canola meal as protein source, with or without 50 g/kg ofA. mearnsiitannin extract (i.e. 15 g/kg of total dietary DM). There was no effect of protein source on most variables. The apparent and true organic matter (OM) digestibilities, as well as neutral detergent fibre (NDF) digestibility were negatively affected by tannin extract inclusion without, however, affecting digestible OM intake. The amount of nitrogen (N) excreted in faeces increased whereas the urinary N excretion decreased in tannin extract treatments. No interaction time × treatment was detected for any rumen variable and no treatment effect was observed for rumen fluid pH and reducing sugars concentration. Rumen fluid concentration of ammonia N was lower for the canola meal plus tannin extract treatment. Rumen concentration ofα-amino compounds was not affected by tannin extract but was higher when canola meal was the protein source. The duodenal flow of OM, total N,α-amino N and non-ammonia non-microbial N increased with tannin extract inclusion, whereas the duodenal flow of microbial N was not affected by treatment. For both protein sources, the amount of most individual amino acids flowing to the duodenum increased due to tannin extract addition. In conclusion, the dietary inclusion of 15 g/kg DM of tannin extract fromA. mearnsiiimproved the amino acid supply independently of whether the protein source was canola meal or soybean meal, without affecting the amino acid profile, to steers fed maize silage plus concentrate, with a minor but significant impact on OM digestibility.

The rumen as a water store in sheep

1964 ◽  
Vol 15 (6) ◽  
pp. 961 ◽  
Author(s):  
JF Hecker ◽  
OE Budtz-Olsen ◽  
M Ostwald
Keyword(s):  
Water Deprivation ◽  
Alimentary Tract ◽  
Rumen Fluid ◽  
Body Weight Loss ◽  
Fluid Volume ◽  
The Body ◽  
Phenol Red ◽  
The Third ◽  
The Mean ◽  
Made In

The rumen fluid volume in sheep was measured by the method of phenol red dilution. Serial determinations made in 22 sheep deprived of food and water for up to 8 days showed that the greatest decrease in rumen fluid volume occurred during the first 2–3 days, the magnitude of the decrease depending on the initial volume. After the third day, the rate of loss of rumen fluid became slower as the rumen fluid volume became depleted. Sheep deprived of food only gave similar results to those deprived of both food and water. This absorption of rumen fluid during the first 2–3 days of food and water deprivation may account for the expansion of plasma volume which has been recorded on the third day. In a group of eight sheep deprived of food and water for 4 days, the mean rumen volume loss for the period amounted to about half the body weight loss. These results support the view that in the sheep, the water balance of the body proper is kept virtually unaltered by fluid drawn from the alimentary tract during the first days of water deprivation. The animal does not become dehydrated, in the physiological sense, until this reserve is depleted. For this reason, the rumen may be regarded as a water "store" in sheep.

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