scholarly journals Comparative In Vitro Resistance of Human Periodontal Bacterial Pathogens to Tinidazole and Four Other Antibiotics

Antibiotics ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 68 ◽  
Author(s):  
Thomas E. Rams ◽  
Jacqueline D. Sautter ◽  
Arie J. van Winkelhoff
Keyword(s):  
Fusobacterium Nucleatum ◽  
Periodontal Pathogens ◽  
Test Species ◽  
Campylobacter Rectus ◽  
Resistant Species ◽  
Streptococcus Constellatus ◽  
Parvimonas Micra ◽  
Severe Periodontitis ◽  
Resistant Strains

The in vitro resistance of selected red/orange complex periodontal pathogens to tinidazole was compared with four other antibiotics. Subgingival biofilm samples from 88 adults with severe periodontitis were anaerobically incubated on enriched Brucella blood agar with and without supplementation with tinidazole (16 mg/L), metronidazole (16 mg/L), amoxicillin (8 mg/L), doxycycline (4 mg/L), or clindamycin (4 mg/L). Growth of Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia/nigrescens, Parvimonas micra, Fusobacterium nucleatum, Streptococcus constellatus, or Campylobacter rectus on antibiotic-supplemented plates indicated their in vitro antibiotic resistance. Tinidazole inhibited all test species, except P. intermedia/nigrescens, P. micra, and S. constellatus in 3.8%, 10.2%, and 88.9% of species-positive patients, respectively. Significantly fewer patients yielded tinidazole-resistant test species, and had significantly lower subgingival proportions of tinidazole-resistant organisms, than patients with amoxicillin, doxycycline, or clindamycin-resistant species, but not those with metronidazole-resistant strains. Joint in vitro species resistance to tinidazole and amoxicillin, or metronidazole and amoxicillin, was rare. Tinidazole performed in vitro similar to metronidazole, and markedly better than amoxicillin, doxycycline, or clindamycin, against fresh clinical isolates of red/orange complex periodontal pathogens. As a result of its similar antimicrobial spectrum, and more convenient once-a-day oral dosing, tinidazole should be considered in place of metronidazole for systemic periodontitis drug therapy.

Antibacterial properties in-vitro of Mexican serviceberry extracts against dental biofilm species

2021 ◽  
pp. 1-16
Author(s):  
Erika-Alejandra Salinas-Peña ◽  
Martha Mendoza-Rodríguez ◽  
Claudia Velázquez-González ◽  
Carlo Eduardo Medina-Solis ◽  
América Patricia Pontigo-Loyola ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Antibacterial Properties ◽  
Fusobacterium Nucleatum ◽  
Positive Control ◽  
Dilution Method ◽  
Periodontal Pathogens ◽  
Leaf Extracts ◽  
Dental Biofilm ◽  
Biofilm Bacteria

BACKGROUND: The Mexican serviceberry, Malacomeles denticulata, have been used as a successful oral therapy by Mexican communities without enough scientific support. OBJECTIVE: To evaluate the M. denticulata extracts with selective antibacterial properties over dental biofilm bacteria. METHODS: Fruit, Leaf, and Stem of M. denticulata extracts were evaluated with micro-broth dilution method using ATCC bacteria. OD600 values had compared against each positive control (T-student-test). Anaerobically viability had confirmed by Colony-Forming-Units. Thin-Layer-Chromatography was used to identify the number of compounds and phytochemicals to identify secondary metabolites of the selected extracts. RESULTS: Streptococcus mutans showed Minimum-Bactericidal-Concentrations_(MBC) at 30 mg/mL to Fruit, Leaf, and Stem extracts. Periodontal-pathogens Aggregatibacter actinomycetemcomitans serotype b_(MBC = 30 mg/mL_p <  0.01); Fusobacterium nucleatum subsp. nucleatum_(MBC = 30 mg/mL_p<0.001); Parvimonas micra_(MBC = 15 mg/mL_NS); Porphyromonas gingivalis_(MBC = 30 mg/mL_NS); and Prevotella intermedia_(MBC = 3.75 mg/mL_NS) presented higher sensitivity to Leaf-Methanol, than the primary colonizers. Phytochemicals showed positive results to anthraquinones, coumarins, flavonoids, saponins, saponins steroids/triterpenoids, steroids/triterpenes, and tannins/phenols. CONCLUSION: We suggest the natural extracts of fruit and leaf of the Mexican serviceberry for the preventive use over the oral cariogenic or periodontal biofilm species, by their selective antibacterial properties against pathogenic species evaluated in-vitro, and due to the presence of antibacterial secondary metabolites identified as flavonoids and saponins of M. denticulata leaf extracts.

scholarly journals Characterization of the Subgingival Cultivable Microbiota in Patients with Different Stages of Periodontitis in Spain and Colombia. A Cross-Sectional Study

2021 ◽  
Vol 9 (9) ◽  
pp. 1940
Author(s):  
Roquelina Pianeta ◽  
Margarita Iniesta ◽  
Diana Marcela Castillo ◽  
Gloria I. Lafaurie ◽  
Mariano Sanz ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Fusobacterium Nucleatum ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Chi Square ◽  
Cross Sectional ◽  
Campylobacter Rectus ◽  
Parvimonas Micra ◽  
Final Sample

The objective was to characterize and compare the subgingival microbiota in patients diagnosed according to the World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions 2018. For this cross-sectional study, Spanish and Colombian subjects (characterized as health/gingivitis, periodontitis in stages I-II or stages III-IV) were clinically assessed, and subgingival samples were taken and processed by culture. The comparisons among patients with periodontal status (and between countries) was made using Mann–Whitney, Kruskal–Wallis, ANOVA and chi-square tests. The final sample consisted of 167 subjects. Eikenella corrodens and Parvimonas micra were more frequently detected in health/gingivitis and Porphyromonas gingivalis in periodontitis (p < 0.05). Higher total counts were observed in Colombia (p = 0.036). In Spain, significantly higher levels of P. gingivalis and Campylobacter rectus were observed, and of Tannerella forsythia, P. micra, Prevotella intermedia, Fusobacterium nucleatum, Actinomyces odontolyticus and Capnocytophaga spp. in Colombia (p < 0.001). P. micra was more prevalent in health/gingivitis and stage I-II periodontitis in Colombia, and P. gingivalis in all periodontitis groups in Spain (p < 0.05). As conclusions, significant differences were detected in the microbiota between health/gingivitis and periodontitis, with minor differences between stages of periodontitis. Differences were also relevant between countries, with Colombia showing larger counts and variability of bacterial species.

scholarly journals Diagnostic Models for Screening of Periodontitis with Inflammatory Mediators and Microbial Profiles in Saliva

Diagnostics ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 820
Author(s):  
Jungwon Lee ◽  
Jun-Beom Lee ◽  
Hyun-Young Song ◽  
Min Jung Son ◽  
Ling Li ◽  
...  
Keyword(s):  
Immunoglobulin A ◽  
Stage Iv ◽  
Fusobacterium Nucleatum ◽  
Operating Characteristics ◽  
Active Matrix ◽  
Campylobacter Rectus ◽  
Reactive Protein ◽  
Salivary Biomarkers ◽  
Parvimonas Micra ◽  
Diagnostic Models

This study aims to investigate and assess salivary biomarkers and microbial profiles as a means of diagnosing periodontitis. A total of 121 subjects were included: 28 periodontally healthy subjects, 24 with Stage I periodontitis, 24 with Stage II, 23 with Stage III, and 22 with Stage IV. Salivary proteins (including active matrix metalloproteinase-8 (MMP-8), pro-MMP-8, total MMP-8, C-reactive protein, secretory immunoglobulin A) and planktonic bacteria (including Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia, Porphyromonas nigrescens, Parvimonas micra, Campylobacter rectus, Eubacterium nodatum, Eikenella corrodens, Streptococcus mutans, Staphylococcus aureus, Enterococcus faecalis, and Actinomyces viscosus) were measured from salivary samples. The performance of the diagnostic models was assessed by receiver operating characteristics (ROCs) and area under the ROC curve (AUC) analysis. The diagnostic models were constructed based on the subjects’ proteins and/or microbial profiles, resulting in two potential diagnosis models that achieved better diagnostic powers, with an AUC value > 0.750 for the diagnosis of Stages II, III, and IV periodontitis (Model PA-I; AUC: 0.796, sensitivity: 0.754, specificity: 0.712) and for the diagnosis of Stages III and IV periodontitis (Model PA-II; AUC: 0.796, sensitivity: 0.756, specificity: 0.868). This study can contribute to screening for periodontitis based on salivary biomarkers.

scholarly journals Subgingival bacterial microbiota associated with ovine periodontitis

2019 ◽  
Vol 39 (7) ◽  
pp. 454-459
Author(s):  
Natália S. Silva ◽  
Ana Carolina Borsanelli ◽  
Elerson Gaetti-Jardim Júnior ◽  
Christiane Marie Schweitzer ◽  
José Alcides S. Silveira ◽  
...  
Keyword(s):  
Anaerobic Bacteria ◽  
Fusobacterium Nucleatum ◽  
Periodontal Pocket ◽  
Susceptible Host ◽  
Gram Negative ◽  
Campylobacter Rectus ◽  
Prevotella Nigrescens ◽  
Bacterial Microbiota ◽  
Severe Periodontitis ◽  
Healthy Sheep

ABSTRACT: Periodontitis is an inflammatory response in a susceptible host caused by complex microbiota, predominantly composed of Gram-negative anaerobic bacteria. Aiming to characterize the subgingival bacterial microbiota associated with ovine periodontitis, polymerase chain reaction (PCR) was performed in subgingival periodontal pocket samples of 14 sheep with severe periodontitis and in subgingival sulcus biofilm of 14 periodontally healthy sheep in search mainly of Gram-negative and Gram-positive microorganisms considered important periodontopathogens. The most prevalent bacteria in the sheep with periodontal lesions were Tannerella forsythia (78.6%), Treponema denticola (78.6%), Fusobacterium nucleatum (64.3%), and Porphyromonas gingivalis (50%), whereas in the healthy sheep, F. nucleatum (42.8%) was the most often detected bacterium. Statistically significant differences were observed for Campylobacter rectus, Enterococcus faecium, Prevotella nigrescens, T. forsythia, and T. denticola (p<0.05) in the sheep with periodontitis in the comparison between groups. Aggregatibacter actinomycetemcomitans, Enterococcus faecalis, and Porphyromonas gulae were not detected in any of the samples analyzed. In conclusion, C. rectus, E. faecium, P. nigrescens, T. forsythia, and T. denticola were associated with severe lesions caused by ovine periodontitis, and F. nucleatum was the most prevalent microorganism in the subgengival sulcus biofilm of healthy sheep.

scholarly journals MICRO BIOLOGY OF PERIODONTAL DISEASE- A REVIEW

2021 ◽  
Vol 5 (6) ◽  
Author(s):  
Jageer Chinna ◽  
Jannat Sharma
Keyword(s):  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Disease Process ◽  
Fusobacterium Nucleatum ◽  
Host Cells ◽  
Bacterial Invasion ◽  
Periodontal Pathogens ◽  
Tissue Destruction ◽  
Campylobacter Rectus ◽  
Periodontal Tissues

Periodontal diseases are inflammatory and destructive diseases of the dentogingival complex associated with specific periodontal pathogens inhabiting periodontal pockets. Periodontal diseases lead to damage of the periodontal tissues supporting the teeth (bone and connective tissue) and affect the quality of life of the affected individuals: poor alimentation, tooth loss, social and financial problems. Although it is generally considered that the disease has multifactorial etiology, data show that some specific Gram-negative microorganisms in the subgingival plaque biofilm play a major role in the initiation and progression of periodontitis. Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia form a consortium in the subgingival biofilm and are regarded as the principal periodontopathogenic bacteria. Other microorganisms that have been implicated as predominant species in the disease process are: Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Prevotella intermedia, Campylobacter rectus, Peptostreptococcus migros, Eikenella corrodens. In periodontitis, the initiation of the disease is the colonization of the tissues by these pathogenic species. The next step is bacterial invasion or invasion by pathogenic products into the periodontal tissues, interactions of bacteria or their substances with host cells, and this directly/indirectly causes degradation of the periodontium, resulting in tissue destruction. Keywords: periodontal disease, periodontal pathogens, microbiology.

scholarly journals New Evidences of Antibacterial Effects of Cranberry Against Periodontal Pathogens

Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 246 ◽  
Author(s):  
María C. Sánchez ◽  
Honorato Ribeiro-Vidal ◽  
Begoña Bartolomé ◽  
Elena Figuero ◽  
M. Victoria Moreno-Arribas ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Periodontal Diseases ◽  
Quantitative Polymerase Chain Reaction ◽  
Chemical Characterization ◽  
Fusobacterium Nucleatum ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Periodontal Pathogens ◽  
Bacteria Adhesion

The worrying rise in antibiotic resistances emphasizes the need to seek new approaches for treating and preventing periodontal diseases. The purpose of this study was to evaluate the antibacterial and anti-biofilm activity of cranberry in a validated in vitro biofilm model. After chemical characterization of a selected phenolic-rich cranberry extract, its values for minimum inhibitory concentration and minimum bactericidal concentration were calculated for the six bacteria forming the biofilm (Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans). Antibacterial activity of the cranberry extract in the formed biofilm was evaluated by assessing the reduction in bacteria viability, using quantitative polymerase chain reaction (qPCR) combined with propidium monoazide (PMA), and by confocal laser scanning microscopy (CLSM), and anti-biofilm activity by studying the inhibition of the incorporation of different bacteria species in biofilms formed in the presence of the cranberry extract, using qPCR and CLSM. In planktonic state, bacteria viability was significantly reduced by cranberry (p < 0.05). When growing in biofilms, a significant effect was observed against initial and early colonizers (S. oralis (p ≤ 0.017), A. naeslundii (p = 0.006) and V. parvula (p = 0.010)) after 30 or 60 s of exposure, while no significant effects were detected against periodontal pathogens (F. nucleatum, P. gingivalis or A. actinomycetemcomitans (p > 0.05)). Conversely, cranberry significantly (p < 0.001 in all cases) interfered with the incorporation of five of the six bacteria species during the development of 6 h-biofilms, including P. gingivalis, A. actinomycetemcomitans, and F. nucleatum. It was concluded that cranberry had a moderate antibacterial effect against periodontal pathogens in biofilms, but relevant anti-biofilm properties, by affecting bacteria adhesion in the first 6 h of development of biofilms.

scholarly journals Antibiotic Resistance of Human Periodontal Pathogen Parvimonas micra Over 10 Years

Antibiotics ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 709
Author(s):  
Thomas E. Rams ◽  
Jacqueline D. Sautter ◽  
Arie J. van Winkelhoff
Keyword(s):  
United States ◽  
Fold Increase ◽  
Periodontal Pathogen ◽  
Antibiotic Concentration ◽  
P Values ◽  
Parvimonas Micra ◽  
Severe Periodontitis ◽  
Patient Groups ◽  
Culture Positive

Changes were evaluated over 10 years in the in vitro resistance of human periodontopathic strains of Parvimonas micra to four antibiotics. Subgingival biofilms culture positive for P. micra from 300 United States adults with severe periodontitis in 2006, and from a similar group of 300 patients in 2016, were plated onto anaerobically incubated enriched Brucella blood agar alone, or supplemented with either doxycycline (4 mg/L), clindamycin (4 mg/L), amoxicillin (8 mg/L), or metronidazole (16 mg/L). P. micra growth on antibiotic-supplemented media indicated in vitro resistance to the evaluated antibiotic concentration. P. micra resistance was significantly more frequent among patients in 2016, as compared to 2006, for doxycycline (11.3% vs. 0.3% patients; 37.7-fold increase), and clindamycin (47.3% vs. 2.0% patients; 23.7-fold increase) (both p < 0.001), whereas resistance to amoxicillin (2.3% vs. 1.0% patients) and metronidazole (0% vs. 0.3% patients) remained low and statistically unchanged between the two patient groups (p-values > 0.05). No P. micra isolates in 2006 or 2016 were jointly resistant in vitro to both amoxicillin and metronidazole. The alarming increases in subgingival P. micra resistance to doxycycline and clindamycin raise serious questions about the empiric use of these antibiotics, either locally or systemically, in the treatment of United States periodontitis patients harboring subgingival P. micra.

Preparation and evaluation of PCLA2575 membranes loaded ornidazole in vitro

2017 ◽  
Vol 32 (6) ◽  
pp. 615-627 ◽  
Author(s):  
Zhengmei Huang ◽  
Shenchun Wang ◽  
Ningtao Wang ◽  
Xiaofei Ma ◽  
Junu Karki ◽  
...  
Keyword(s):  
Drug Release ◽  
Sustained Release ◽  
Fusobacterium Nucleatum ◽  
Antibacterial Activities ◽  
Inhibitory Effect ◽  
Release Time ◽  
Periodontal Pathogens ◽  
Obvious Effect ◽  
Solid Culture Medium

The aim of this study was to evaluate the properties of the sustained release and antibacterial activity of the ornidazole-drug-loaded membranes using poly[(ethylene glycol)-caprolactone-lactide] (PCLA2575) as membrane material. Ornidazole-loaded membranes were prepared by solvent casting method with the proportion of 5 wt%, 8 wt%, and 10 wt%, respectively. In vitro drug release properties were determined by ultraviolet spectrophotometric method. The antibacterial activities against Streptococcus mutans and Fusobacterium nucleatum in vitro were observed on solid culture medium. The membrane had the high drug loadings and slow-release performance. Drug release time was shortened with the increase in the content of ornidazole, but all of them can achieve more than 7 days. The membrane had strong inhibitory effect on both S. mutans and F. nucleatum. As drug content increased, the antibacterial activities also increased. The membrane had better inhibitory effect on F. nucleatum than S. mutans. Therefore, the ornidazole drug-loaded membrane is expected to be used for the treatment of periodontal disease because of the obvious effect of periodontal pathogens inhibition and good sustained-release performance.

Microbial diversity of the supra- and subgingival biofilm of healthy individuals after brushing with chlorhexidine- or silver-coated toothbrush bristles

2015 ◽  
Vol 61 (2) ◽  
pp. 112-123 ◽  
Author(s):  
Cássio do Nascimento ◽  
Diana Ferreira Paulo ◽  
Murillo Sucena Pita ◽  
Vinícius Pedrazzi ◽  
Rubens Ferreira de Albuquerque Junior
Keyword(s):  
Fusobacterium Nucleatum ◽  
Control Group ◽  
Oral Microbiota ◽  
Periodontal Pathogens ◽  
Healthy Individuals ◽  
Individual Genome ◽  
Prevotella Nigrescens ◽  
Parvimonas Micra ◽  
The Individual ◽  
Healthy Participants

Nanoparticulate silver has recently been reported as an effective antimicrobial agent. The aim of this clinical study was to investigate the potential changes on the oral microbiota of healthy individuals after controlled brushing with chlorhexidine- or silver-coated toothbrush bristles. Twenty-four healthy participants were enrolled in this investigation and randomly submitted to 3 interventions. All the participants received, in a crossover format, the following toothbrushing interventions: (i) chlorhexidine-coated bristles, (ii) silver-coated bristles, and (iii) conventional toothbrush (Control). All the interventions had a duration of 30 days. The DNA checkerboard hybridization method was used to identify and quantify up to 43 microbial species colonizing the supra- and subgingival biofilm. The supragingival samples presented higher genome counts than the subgingival samples (p < 0.0001). The total genome counts from the Control group showed the highest values, followed by the silver and chlorhexidine groups (p < 0.0001). After 4 weeks of brushing, the silver-coated and chlorhexidine-coated bristles were capable of reducing or maintaining lower levels of the bacterial counts of the putative periodontal pathogens Tanerella forsythia, Treponema denticola, and Porphyromonas gingivalis. Other major periodontal pathogens, such as Prevotella intermedia, Fusobacterium nucleatum, Prevotella nigrescens, and Parvimonas micra, were also detected at lower levels. The toothbrush bristles impregnated with silver nanoparticles reduced the total and individual genome count in the supra- and subgingival biofilm after 4 weeks of brushing. Chlorhexidine was not effective in reducing the total genome counts in both supra- or subgingival biofilm after 4 weeks of brushing. Chlorhexidine reduced the individual genome counts in the supragingival biofilm for most of the target species, including putative periodontal pathogens.

scholarly journals NEW OPPORTUNITIES LOCAL MEDICAL TREATMENT OF PERIODONTAL DISEASES (MICROBIOLOGICAL RATIONALE)

2018 ◽  
Vol 22 (4) ◽  
pp. 180-183
Author(s):  
A. N Kalinina ◽  
I. S Lasko ◽  
V. N Tsarev ◽  
Egor Evgen'evich Olesov ◽  
A. F Stepanov ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Stationary Phase ◽  
Periodontal Diseases ◽  
Fusobacterium Nucleatum ◽  
Lag Phase ◽  
Periodontal Pathogens ◽  
Streptococcus Constellatus ◽  
Comparison Criteria ◽  
Peak Phase ◽  
Geometric Growth

The article presents the results of a microbiological experiment to study the sensitivity of periodontal pathogens to coniferous polyprenols in the preparation of “Solagift”. The optica density of clinical isolates Streptococcus constellatus; Staphylococcus aureus; Fusobacterium nucleatum; Aggregatibacter actinomycetemcomitans during cultivation with the addition of polyprenol concentrate 1:5 was measured during 3-7 days. Comparison criteria: the change in the phase of adaptation (lag-phase), the phase change of geometric growth, the amplitude of the peak phase of geometric growth, the duration of the stationary phase, the period of the withering away of culture. In comparison with the parameters of periodontal pathogens in the control, the presence of coniferous polyprenols led to a significant decrease in the activity of all microbes according to all criteria, especially Staphylococcus aureus, whose growth was completely suppressed.

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