scholarly journals New Neuroprotective Effect of Lemon IntegroPectin on Neuronal Cellular Model

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 669
Author(s):  
Domenico Nuzzo ◽  
Pasquale Picone ◽  
Costanza Giardina ◽  
Miriana Scordino ◽  
Giuseppa Mudò ◽  
...  
Keyword(s):  
Neurological Diseases ◽  
Neuroprotective Effect ◽  
Hydrodynamic Cavitation ◽  
Neuroprotective Activity ◽  
Processing Waste ◽  
Citrus Pectin ◽  
Pectic Substance ◽  
Cellular Processes ◽  
Mitochondrial Regulation

Lemon IntegroPectin obtained via hydrodynamic cavitation of organic lemon processing waste in water shows significant neuroprotective activity in vitro, as first reported in this study investigating the effects of both lemon IntegroPectin and commercial citrus pectin on cell viability, cell morphology, reactive oxygen species (ROS) production, and mitochondria perturbation induced by treatment of neuronal SH-SY5Y human cells with H2O2. Mediated by ROS, including H2O2 and its derivatives, oxidative stress alters numerous cellular processes, such as mitochondrial regulation and cell signaling, propagating cellular injury that leads to incurable neurodegenerative diseases. These results, and the absence of toxicity of this new pectic substance rich in adsorbed flavonoids and terpenes, suggest further studies to investigate its activity in preventing, retarding, or even curing neurological diseases.

scholarly journals Neuroprotective and Mitoprotective Effects of Lemon IntegroPectin on SH-SY5Y Cells

2021 ◽  
Author(s):  
Domenico Nuzzo ◽  
Pasquale Picone ◽  
Costanza Giardina ◽  
Miriana Scordino ◽  
Giuseppa Mudò ◽  
...  
Keyword(s):  
Neurological Diseases ◽  
Hydrodynamic Cavitation ◽  
Neuroprotective Activity ◽  
Oxygen Species ◽  
Processing Waste ◽  
Citrus Pectin ◽  
Pectic Substance ◽  
Cellular Processes ◽  
Mitochondrial Regulation

AbstractLemon IntegroPectin obtained via hydrodynamic cavitation of organic lemon processing waste in water shows significant neuroprotective activity in vitro, as first reported in this study investigating the effects of both lemon IntegroPectin and commercial citrus pectin on cell viability, cell morphology, reactive oxygen species (ROS) production and mitochondria perturbation induced by treatment of neuronal SH-SY5Y human cells with H2O2. Mediated by ROS including H2O2 and its derivatives, oxidative stress alters numerous cellular processes, including mitochondrial regulation and cell signaling, propagating cellular injury that leads to incurable neurodegenerative diseases. These results, and the absence of toxicity of this new pectic substance rich in adsorbed flavonoids and terpenes, support further investigations to verify its activity in preventing, retarding, or even curing neurological diseases.

scholarly journals A gluten-free biscuit fortified with lemon IntegroPectin

2021 ◽  
Author(s):  
Domenico Nuzzo ◽  
Antonino Scurria ◽  
Pasquale Picone ◽  
Alessandro Guiducci ◽  
Mario Pagliaro ◽  
...  
Keyword(s):  
Chronic Disease ◽  
Antioxidant Properties ◽  
Rice Flour ◽  
Hydrodynamic Cavitation ◽  
Neuroprotective Activity ◽  
Gluten Free ◽  
Processing Waste ◽  
Citrus Pectin ◽  
Neuronal Disorders

Abstract We report the first outcomes of producing a gluten-free biscuit by replacing 2.5 wt% of the rice flour used in the preparation of the cookie with lemon IntegroPectin, a new citrus pectin obtained from lemon processing waste via hydrodynamic cavitation showing exceptional antioxidant properties and (in vitro) high neuroprotective activity. The cookie’s friability, palate adhesion, flavor persistency and compactness remain virtually unchanged. Only the sweetness and the smell (flavor) of the functionalized cookie are lower than those of the commercial biscuit. Production of biscuits fortified with this new pectin might result not only in gluten-free and low-calorie cookies but also in a fortified cookie capable to aid in the prevention of chronic disease such as neuronal disorders.

scholarly journals Mumijo Traditional Medicine: Fossil Deposits from Antarctica (Chemical Composition and Beneficial Bioactivity)

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Anna Aiello ◽  
Ernesto Fattorusso ◽  
Marialuisa Menna ◽  
Rocco Vitalone ◽  
Heinz C. Schröder ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Traditional Medicine ◽  
Cortical Neurons ◽  
Neuroprotective Effect ◽  
Amyloid Peptide ◽  
Neuroprotective Activity ◽  
Antiallergic Activity ◽  
Neuronal Pc12 Cells ◽  
Biomedical Potential

Mumijo is a widely used traditional medicine, especially in Russia, Altai Mountains, Mongolia, Iran Kasachstan and in Kirgistan. Mumijo preparations have been successfully used for the prevention and treatment of infectious diseases; they display immune-stimulating and antiallergic activity as well. In the present study, we investigate the chemical composition and the biomedical potential of a Mumijo(-related) product collected from the Antarctica. The yellow material originates from the snow petrels,Pagodroma nivea. Extensive purification and chemical analysis revealed that the fossil samples are a mixture of glycerol derivatives.In vitroexperiments showed that the Mumijo extract caused in cortical neurons a strong neuroprotective effect against the apoptosis-inducing amyloid peptide fragmentβ-fragment 25–35 (Aβ25–35). In addition, the fraction rich in glycerol ethers/wax esters displayed a significant growth-promoting activity in permanent neuronal PC12 cells. It is concluded that this new Mumijo preparation has distinct and marked neuroprotective activity, very likely due to the content of glycerol ether derivatives.

scholarly journals Epigallocatechin-3-Gallate-Loaded Liposomes Favor Anti-Inflammation of Microglia Cells and Promote Neuroprotection

2021 ◽  
Vol 22 (6) ◽  
pp. 3037
Author(s):  
Chun-Yuan Cheng ◽  
Lassina Barro ◽  
Shang-Ting Tsai ◽  
Tai-Wei Feng ◽  
Xiao-Yu Wu ◽  
...  
Keyword(s):  
Green Tea ◽  
Neuroprotective Effect ◽  
Motor Impairment ◽  
Cell Uptake ◽  
Microglial Cells ◽  
Neuroprotective Activity ◽  
Sprague Dawley ◽  
Disease Modifying Therapy

Microglia-mediated neuroinflammation is recognized to mainly contribute to the progression of neurodegenerative diseases. Epigallocatechin-3-gallate (EGCG), known as a natural antioxidant in green tea, can inhibit microglia-mediated inflammation and protect neurons but has disadvantages such as high instability and low bioavailability. We developed an EGCG liposomal formulation to improve its bioavailability and evaluated the neuroprotective activity in in vitro and in vivo neuroinflammation models. EGCG-loaded liposomes have been prepared from phosphatidylcholine (PC) or phosphatidylserine (PS) coated with or without vitamin E (VE) by hydration and membrane extrusion method. The anti-inflammatory effect has been evaluated against lipopolysaccharide (LPS)-induced BV-2 microglial cells activation and the inflammation in the substantia nigra of Sprague Dawley rats. In the cellular inflammation model, murine BV-2 microglial cells changed their morphology from normal spheroid to activated spindle shape after 24 h of induction of LPS. In the in vitro free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, EGCG scavenged 80% of DPPH within 3 min. EGCG-loaded liposomes could be phagocytized by BV-2 cells after 1 h of cell culture from cell uptake experiments. EGCG-loaded liposomes improved the production of BV-2 microglia-derived nitric oxide and TNF-α following LPS. In the in vivo Parkinsonian syndrome rat model, simultaneous intra-nigral injection of EGCG-loaded liposomes attenuated LPS-induced pro-inflammatory cytokines and restored motor impairment. We demonstrated that EGCG-loaded liposomes exert a neuroprotective effect by modulating microglia activation. EGCG extracted from green tea and loaded liposomes could be a valuable candidate for disease-modifying therapy for Parkinson’s disease (PD).

scholarly journals Placental stem cells, organotypic culture and human placenta extract have neuroprotective activity in vitro

2017 ◽  
Vol 5 (1) ◽  
pp. 39-42 ◽  
Author(s):  
V. Prokopyuk ◽  
O. Chub ◽  
M. Shevchenko ◽  
O. Prokopyuk
Keyword(s):  
Stem Cells ◽  
Organotypic Culture ◽  
Neuroprotective Effect ◽  
In Vitro Models ◽  
Glutamate Excitotoxicity ◽  
Neuroprotective Activity ◽  
Neural Cells ◽  
Before And After ◽  
Placental Extract

According to WHO, 6.7 million people die from stroke every year. The search for new neuroprotective substances remains an urgent task.The purpose of this study was to investigate the neuroprotective activity of factors of placental origin.Materials and methods. Neuroprotective activity of media conditioned with cryopreserved placenta derived mesenchymal stem cells (MSCs), organotypic culture of placenta and placental extract was studied on in vitro models of glutamate excitotoxicity in rats` neural cells. Neural cells were cultured with placental factors without glutamate treatment, before and after glutamate treatment. Neural cells` metabolic activity was assessed by MTT test.Results. Placental factors increase the MTT test indexes, prevent the toxic effect of glutamate on neural cells and promote their recovery. The thermolability of factors of placental origin and the effectiveness of various placental preparations are shown.Conclusions. Conditional media of placenta derived MSCs, organotypic culture of the placenta and human placental extract have neuroprotective effect on rats` brain cells in vitro.

scholarly journals Neuroprotective Properties of Kempferol Derivatives from Maesa membranacea against Oxidative Stress-Induced Cell Damage: An Association with Cathepsin D Inhibition and PI3K/Akt Activation

2021 ◽  
Vol 22 (19) ◽  
pp. 10363
Author(s):  
Danuta Jantas ◽  
Janusz Malarz ◽  
Thanh Nguyen Le ◽  
Anna Stojakowska
Keyword(s):  
Cathepsin D ◽  
Cell Damage ◽  
Neuroprotective Effect ◽  
Radical Scavenging ◽  
Neuroblastoma Cells ◽  
Neuroprotective Activity ◽  
Major Constituent ◽  
Neuroprotective Effects ◽  
Potential Health

As components of the human diet with potential health benefits, flavonols are the subject of numerous studies, confirming their antioxidant, free radical scavenging and anti-inflammatory activity. Taking into consideration the postulated pathogenesis of certain CNS dysfunctions characterized by neuronal degradation, flavonols may prevent the decay of neurons in multiple pathways. Leaves of Maesa membranacea yielded several flavonol glycosides including α-rhamnoisorobin (kaempferol 7-O-α-rhamnoside) and kaempferitrin (kaempferol 3,7-di-O-α-rhamnoside). The latter compound was a major constituent of the investigated plant material. Neuroprotective effects of kaempferitrin and α-rhamnoisorobin were tested in vitro using H2O2-, 6-OHDA- and doxorubicin-induced models of SH-SY5Y cell damage. Both undifferentiated and differentiated neuroblastoma cells were used in the experiments. α-Rhamnoisorobin at a concentration range of 1–10 µM demonstrated cytoprotective effects against H2O2-induced cell damage. The compound (at 1–10 µM) was also effective in attenuating 6-OHDA-induced neurotoxicity. In both H2O2- and 6-OHDA-induced cell damage, kaempferitrin, similar to isoquercitrin, demonstrated neuroprotective activity at the highest of the tested concentrations (50 µM). The tested flavonols were not effective in counteracting doxorubicin-induced cytotoxicity. Their caspase-3- and cathepsin D-inhibitory activities appeared to be structure dependent. Inhibition of the PI3-K/Akt pathway abolished the neuroprotective effect of the investigated flavonols.

scholarly journals Red Ginseng Extract Attenuates Kainate-Induced Excitotoxicity by Antioxidative Effects

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Jin-Yi Han ◽  
Sun-Young Ahn ◽  
Eun-Hye Oh ◽  
Sang-Yoon Nam ◽  
Jin Tae Hong ◽  
...  
Keyword(s):  
Hippocampal Neurons ◽  
Mossy Fiber ◽  
Neuroprotective Effect ◽  
Order Rate Constant ◽  
Neuroprotective Activity ◽  
Dependent Manner ◽  
Red Ginseng ◽  
Ginseng Extract

This study investigated the neuroprotective activity of red ginseng extract (RGE,Panax ginseng, C. A. Meyer) against kainic acid- (KA-) induced excitotoxicityin vitroandin vivo. In hippocampal cells, RGE inhibited KA-induced excitotoxicity in a dose-dependent manner as measured by the MTT assay. To study the possible mechanisms of the RGE-mediated neuroprotective effect against KA-induced cytotoxicity, we examined the levels of intracellular reactive oxygen species (ROS) and [Ca2+]iin cultured hippocampal neurons and found that RGE treatment dose-dependently inhibited intracellular ROS and [Ca2+]ielevation. Oral administration of RGE (30 and 200 mg/kg) in mice decreased the malondialdehyde (MDA) level induced by KA injection (30 mg/kg, i.p.). In addition, similar results were obtained after pretreatment with the radical scavengers Trolox andN,N′-dimethylthiourea (DMTU). Finally, after confirming the protective effect of RGE on hippocampal brain-derived neurotropic factor (BDNF) protein levels, we found that RGE is active compounds mixture in KA-induced hippocampal mossy-fiber function improvement. Furthermore, RGE eliminated 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and the IC50was approximately 10 mg/ml. The reductive activity of RGE, as measured by reaction with hydroxyl radical (•OH), was similar to trolox. The second-order rate constant of RGE for•OH was 3.5–4.5×109 M−1·S−1. Therefore, these results indicate that RGE possesses radical reduction activity and alleviates KA-induced excitotoxicity by quenching ROS in hippocampal neurons.

scholarly journals Neuroprotective Effect of Terpenoids Recovered from Olive Oil By-Products

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1507
Author(s):  
Zully J. Suárez Montenegro ◽  
Gerardo Álvarez-Rivera ◽  
José David Sánchez-Martínez ◽  
Rocío Gallego ◽  
Alberto Valdés ◽  
...  
Keyword(s):  
Olive Oil ◽  
Neuroprotective Effect ◽  
Neuroprotective Activity ◽  
Olive Leaves ◽  
Systematic Analysis ◽  
Selective Enrichment ◽  
Natural Extracts ◽  
Flight Mass Spectrometry ◽  
By Products

The neuroprotective potential of 32 natural extracts obtained from olive oil by-products was investigated. The online coupling of supercritical fluid extraction (SFE) and dynamic adsorption/desorption allowed the selective enrichment of olive leaves extracts in different terpenoids’ families. Seven commercial adsorbents based on silica gel, zeolite, aluminum oxide, and sea sand were used with SFE at three different extraction times to evaluate their selectivity towards different terpene families. Collected fractions were analyzed by gas chromatography coupled to quadrupole-time-of-flight mass spectrometry (GC-QTOF-MS) to quantify the recoveries of monoterpenes (C10), sesquiterpenes (C15), diterpenes (C20), and triterpenes (C30). A systematic analysis of the neuroprotective activity of the natural extracts was then carried out. Thus, a set of in vitro bioactivity assays including enzymatic (acetylcholinesterase (AChE), butyrylcholinesterase (BChE)), and anti-inflammatory (lipoxidase (LOX)), as well as antioxidant (ABTS), and reactive oxygen and nitrogen species (ROS and RNS, respectively) activity tests were applied to screen for the neuroprotective potential of these extracts. Statistical analysis showed that olive leaves adsorbates from SS exhibited the highest biological activity potential in terms of neuroprotective effect. Blood–brain barrier permeation and cytotoxicity in HK-2 cells and human THP-1 monocytes were studied for the selected olive leaves fraction corroborating its potential.

scholarly journals Efek Neuroprotektif Fraksi Air Daun Semanggi (Marsilea crenata Presl.) secara In Vitro

2021 ◽  
Vol 2 (1) ◽  
pp. 10-17
Author(s):  
Burhan Ma'arif ◽  
Menara Muslim ◽  
Anisa Eka Riyanti ◽  
Abdul Malik Guhir ◽  
Dilla Amalia ◽  
...  
Keyword(s):  
Neuroprotective Effect ◽  
Estrogen Deficiency ◽  
Inflammatory Activity ◽  
Neuroprotective Activity ◽  
Water Fraction ◽  
Mhc Ii ◽  
Inflammatory Conditions ◽  
Ifn Γ

Background: Estrogen deficiency can trigger several diseases, one of which is neurodegenerative. Neurodegenerative begins with neuroinflammation, which triggers the activation of microglia cells resulting in a pro-inflammatory activity. Potential and relatively safe therapy to use to overcome it is by using phytoestrogen compounds. Marsilea crenata Presl. is a plant that contains phytoestrogens. Objective: The purpose of this study was to analyze the neuroprotective effect of the water fraction of Marsilea crenata Presl. leaves which was shown by inhibition of neuroinflammation and marked by increased levels of MHC II against HMC3 microglia cells. Methods: IFN-γ is induced into HMC3 microglia cells for 24 hours to cause inflammatory conditions. Marsilea crenata Presl. leaves water fraction was given at a dose of 62,5; 125; and 250 µg / ml. Analysis of the neuroprotective effect of HMC3 microglia cells using the ICC method with the aid of the CLSM instrument. Results: The results of this study indicate that the water fraction of Marsilea crenata Presl. leaves can reduce MHC II expression at concentrations of 125 and 250 μg / ml with values ​​of 465,748 and 460,884 AU at p<0,005. Conclusion: This study concludes that the water fraction of Marsilea crenata Presl. has neuroprotective activity shown at doses of 125 and 250 μg/ml which can reduce MHC II expression in HMC3 microglia cells induced by IFN-γ. The ED50 value which has a neuroprotective effect is at a dose of 0,582 μg/ml.  

scholarly journals Effect of Different Processing Methods on Phytochemical Contents and Neuroprotective Activity of Camellia euphlebia Leaves Extract

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Dongye He ◽  
Shu Jia ◽  
Yongping Xu
Keyword(s):  
Particle Size ◽  
Neuroprotective Effect ◽  
Antidepressant Activity ◽  
Neuroprotective Activity ◽  
Powder Particle Size ◽  
Shell Powder ◽  
Phytochemical Contents ◽  
Camellia Euphlebia

Camellia euphlebia is a new food source and traditional folk medicine in China. Previous studies have demonstrated the antidepressant activity of Camellia euphlebia extract by both in vivo and in vitro experiments. The effects of different pretreatments on phytochemical contents and neuroprotective activity of Camellia euphlebia extract were further investigated in order to develop an optimal processing method that makes the extraction more efficient. Six different powders of Camellia euphlebia leaves were prepared by different pretreatments. The particle size and morphology were examined by using a Malvern particle size analyzer and scanning electron microscopy, respectively. The results showed that the percentage of powder particle size within a range of 0.2∼40 μm was up to 79.18% after press-shear assisted interaction technology pretreatment by 2% addition of shellfish shell powder, and the cells were broken completely. Additionally, the contents of flavonoids, polysaccharides, polyphenols, saponins, and catechin in the extract were 11.78 ± 0.62%, 34.60 ± 3.37%, 6.15 ± 0.29%, 9.43 ± 1.19%, and 1.99 ± 0.11%, respectively, which were higher than those of the other five extracts. Moreover, the extract had the strongest neuroprotective activity by comparing the neuroprotective effect of different extracts on corticosterone-induced neurotoxicity in differentiated PC12 cells. It is concluded that press-shear assisted interaction technology with 2% addition of shellfish shell powder pretreatment, to a great extent, improved the dissolution of bioactive ingredients in Camellia euphlebia.

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