Hibiscus Flower and Olive Leaf Extracts Activate Apoptosis in SH-SY5Y Cells

Compounds of natural origin may constitute an interesting tool for the treatment of neuroblastoma, the most prevalent extracranial solid tumor in children. PRES is a commercially available food supplement, composed of a 13:2 (w/w) extracts mix of Olea europaea L. leaves (OE) and Hibiscus sabdariffa L. flowers (HS). Its potential towards neuroblastoma is still unexplored and was thus investigated in human neuroblastoma SH-SY5Y cells. PRES decreased the viability of cells in a concentration-dependent fashion (24 h IC50 247.2 ± 31.8 µg/mL). Cytotoxicity was accompanied by an increase in early and late apoptotic cells (AV-PI assay) and sub G0/G1 cells (cell cycle analysis), ROS formation, reduction in mitochondrial membrane potential, and caspases activities. The ROS scavenger N-acetyl-L-cysteine reverted the cytotoxic effects of PRES, suggesting a key role played by ROS in PRES-mediated SH-SY5Y cell death. Finally, the effects of OE and HS extracts were singularly tested and compared to those of the corresponding mixture. OE- or HS-mediated cytotoxicity was always significantly lower than that caused by PRES, suggesting a synergic effect. In conclusion, the present findings highlight the potential of PRES for the treatment of neuroblastoma and offers the basis for a further characterization of the mechanisms underlying its effects.

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Characterization of bioactive compounds in commercial olive leaf extracts, and olive leaves and their infusions

Food & Function ◽

10.1039/c9fo00698b ◽

2019 ◽

Vol 10 (8) ◽

pp. 4716-4724 ◽

Cited By ~ 6

Author(s):

Eduardo Medina ◽

Concepción Romero ◽

Pedro García ◽

Manuel Brenes

Keyword(s):

Bioactive Compounds ◽

Olive Leaf ◽

Olive Leaves ◽

Leaf Extracts

A large spectrum of beneficial health properties has been attributed to olive leaves.

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Characterization of compounds and quantitative analysis of oleuropein in commercial olive leaf extracts

Journal of Applied Biological Chemistry ◽

10.3839/jabc.2021.017 ◽

2021 ◽

Vol 64 (2) ◽

pp. 113-119

Author(s):

Mi Hyeon Park ◽

Doo-Young Kim ◽

Alfan Danny Arbianto ◽

Jung-Hee Kim ◽

Seong Mi Lee ◽

...

Keyword(s):

Quantitative Analysis ◽

Olive Leaf ◽

Leaf Extracts

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Genetic diversity, variability and characterization of the agro-morphological traits of Northern Ghana Roselle (Hibiscus sabdariffa var. altissima) accessions

African Journal of Plant Science ◽

10.5897/ajps2019.1783 ◽

2019 ◽

Vol 13 (6) ◽

pp. 168-184

Author(s):

Y. Tetteh Antonia ◽

A. Ankrah Nii ◽

Coffie Nancy ◽

Niagiah Achana

Keyword(s):

Genetic Diversity ◽

Morphological Traits ◽

Northern Ghana ◽

Hibiscus Sabdariffa

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Addition of Olive Leaf Extract to a Mixture of Algae and Extra Virgin Olive Oils Decreases Fatty Acid Oxidation and Synergically Attenuates Age-Induced Hypertension, Sarcopenia and Insulin Resistance in Rats

Antioxidants ◽

10.3390/antiox10071066 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1066

Author(s):

Daniel González-Hedström ◽

María de la Fuente-Fernández ◽

Teresa Priego ◽

Ana Isabel Martín ◽

Sara Amor ◽

...

Keyword(s):

Insulin Resistance ◽

Vascular Function ◽

Virgin Olive Oil ◽

Serum Levels ◽

Acid Oxidation ◽

Antioxidant Compounds ◽

Mrna Levels ◽

Omega 3 ◽

Olive Leaf ◽

Leaf Extracts

Olive-derived products, such as virgin olive oil (EVOO) and/or olive leaf extracts (OLE), exert anti-inflammatory, insulin-sensitizing and antihypertensive properties and may be useful for stabilizing omega 3 fatty acids (n-3 PUFA) due to their high content in antioxidant compounds. In this study, the addition of OLE 4:0.15 (w/w) to a mixture of algae oil (AO) rich in n-3 PUFA and EVOO (25:75, w/w) prevents peroxides formation after 12 months of storage at 30 °C. Furthermore, the treatment with the oil mixture (2.5 mL/Kg) and OLE (100 mg/Kg) for 24 month old Wistar rats in 21 days improved the lipid profile, increased the HOMA-IR and decreased the serum levels of miRNAs 21 and 146a. Treatment with this new nutraceutical also prevented age-induced insulin resistance in the liver, gastrocnemius and visceral adipose tissue by decreasing the mRNA levels of inflammatory and oxidative stress markers. Oil mixture + OLE also attenuated the age-induced alterations in vascular function and prevented muscle loss by decreasing the expression of sarcopenia-related markers. In conclusion, treatment with a new nutraceutical based on a mixture of EVOO, AO and OLE is a useful strategy for improving the stability of n-3 PUFA in the final product and to attenuate the cardiometabolic and muscular disorders associated with aging.

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The Phytochemical Analysis of Vinca L. Species Leaf Extracts Is Correlated with the Antioxidant, Antibacterial, and Antitumor Effects

Molecules ◽

10.3390/molecules26103040 ◽

2021 ◽

Vol 26 (10) ◽

pp. 3040

Author(s):

Alexandra Ciorîță ◽

Cezara Zăgrean-Tuza ◽

Augustin C. Moț ◽

Rahela Carpa ◽

Marcel Pârvu

Keyword(s):

Bacterial Infections ◽

Inhibitory Effect ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Antitumor Effects ◽

Cytotoxic Effects ◽

E Coli ◽

Lactate Dehydrogenase Release ◽

Nitric Oxide Concentration

The phytochemical analysis of Vinca minor, V. herbacea, V. major, and V. major var. variegata leaf extracts showed species-dependent antioxidant, antibacterial, and cytotoxic effects correlated with the identified phytoconstituents. Vincamine was present in V. minor, V. major, and V. major var. variegata, while V. minor had the richest alkaloid content, followed by V. herbacea. V. major var. variegata was richest in flavonoids and the highest total phenolic content was found in V. herbacea which also had elevated levels of rutin. Consequently, V. herbacea had the highest antioxidant activity followed by V. major var. variegata. Whereas, the lowest one was of V. major. The V. minor extract showed the most efficient inhibitory effect against both Staphylococcus aureus and E. coli. On the other hand, V. herbacea had a good anti-bacterial potential only against S. aureus, which was most affected at morphological levels, as indicated by scanning electron microscopy. The Vinca extracts acted in a dose-depended manner against HaCaT keratinocytes and A375 melanoma cells and moreover, with effects on the ultrastructure, nitric oxide concentration, and lactate dehydrogenase release. Therefore, the Vinca species could be exploited further for the development of alternative treatments in bacterial infections or as anticancer adjuvants.

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