Microwave Assisted Extraction (MAE) Process of Tannin from Mangrove Propagules Waste as Natural Dye for Coloring Batik tulis

Mangrove propagule waste is one of the biomass wastes that decays fast, hence it can pollute the environment. Mangrove propagule contains 30.43% tannin, and thus it has high potential to be used as a natural dye for coloringBatik Tulis. Unfortunately, this raw material has not been utilized optimally. The objective of this study is to extract tannin substances from mangrove propagules by using Microwave Assisted Extraction (MAE) method. The effects of solvent types (i.e. ethanol and distilled water), material-solvent ratio, extraction time and temperature on the yield of tannin obtained have also been investigated. The extraction results were analyzed by FT-IR to determine the presence of tannin contained in the dyes. The extraction results showed that the optimum yield of etanol solvent was 27.5%, which occured at material-solvent ratio of 0.04 g/ml, extraction time of 30 minutes, and temperature of 60°C. Whereas, the distilled water produced 22.7% yield of tannin, which also occured at material and solvent ratio 0.04 g/ml, extraction time of 30 minutes, and temperature of 80°C. Additionally, FT-IR analysis of tannin extract from mangrove propagules with etanol and distilled water showed the presence of hydroxyl groups (O-H), groups (C-H), C = C groups, and C-O groups.

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Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity

Antioxidants ◽

10.3390/antiox8050129 ◽

2019 ◽

Vol 8 (5) ◽

pp. 129 ◽

Cited By ~ 11

Author(s):

Bao Le ◽

Kirill S. Golokhvast ◽

Seung Hwan Yang ◽

Sangmi Sun

Keyword(s):

Antioxidant Activity ◽

Superoxide Dismutase ◽

Raw Material ◽

Ulva Pertusa ◽

Extraction Time ◽

Oxidative Enzymes ◽

Microwave Assisted Extraction ◽

Extraction Technology ◽

Microwave Assisted ◽

Assisted Extraction

The use of green marine seaweed Ulva spp. as foods, feed supplements, and functional ingredients has gained increasing interest. Microwave-assisted extraction technology was employed to improve the extraction yield and composition of Ulva pertusa polysaccharides. The antioxidant activity of ulvan was also evaluated. The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated. The chemical structure of different polysaccharides fractions was investigated via FT-IR and the determination of their antioxidant activities. A response surface methodology based on a Box–Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381. Ulvan exerted a strong antioxidant effect against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and showed reducing power in vitro. Ulvan protected RAW 264.7 cells against H2O2-induced oxidative stress by upregulating the expression and enhancing the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT). The results suggest that the polysaccharides from U. pertusa might be promising bioactive compounds for commercial use.

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EKSTRAKSI TANIN DARI KULIT KAYU AKASIA DENGAN MENGGUNAKAN MICROWAVE: PENGARUH DAYA MICROWAVE, WAKTU EKSTRAKSI DAN JENIS PELARUT

Jurnal Teknik Kimia USU ◽

10.32734/jtk.v6i3.1590 ◽

2017 ◽

Vol 6 (3) ◽

pp. 52-57

Author(s):

Iriany ◽

Florentina Pandiangan ◽

Christina Eka P

Keyword(s):

Atomic Absorption ◽

Adsorption Capacity ◽

Microwave Power ◽

Polar Solvent ◽

Extraction Time ◽

Solvent Ratio ◽

Microwave Assisted Extraction ◽

Power Extraction ◽

Microwave Assisted ◽

Assisted Extraction

Tannin is one of complex polyphenol compound which soluble in polar solvent. Tannin could be extracted from acacia bark. This study aims to examine microwave-assisted extraction of tannins under influence of microwave power, extraction time, and solvent and its application to adsorb Cd and Cu. Tannin was extracted from acacia bark with feed-to-solvent ratio (1:20 g/ml). Power of microwave was adjusted 100 W, 180 W, 300 W, 450 W, and 600 W for 1 minute, 3 minutes and 5 minutes respectively using aquadest and ethanol as the solvent. The extracts were analyzed using UV-Vis spectrophotometer. Furthermore, the extract with the highest yield of tannins are used as the adsorbent. The concentration of Cd and cu were analyzed using Atomic Absorption Spectrophotometer. The result showed the highest yield of tannin 26.606 mg/g at 100 W and 3 minutes extraction time using ethanol as the solvent. The result showed that the adsorption capacity of adsorbent from tannin was 3.81 mg/g and 2.26 mg/g for Cd and Cu respectively.

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Microwave-assisted extraction of phenolic compounds from Moringa oleifera seed as anti-biofouling agents in membrane processes

MATEC Web of Conferences ◽

10.1051/matecconf/201820403003 ◽

2018 ◽

Vol 204 ◽

pp. 03003 ◽

Cited By ~ 3

Author(s):

Ni'matul Izza ◽

Shinta Rosalia Dewi ◽

Ashried Setyanda ◽

Agung Sukoyo ◽

Panggulu Utoro ◽

...

Keyword(s):

Moringa Oleifera ◽

Phenolic Content ◽

Extraction Time ◽

Total Phenolic ◽

Solvent Ratio ◽

Microwave Assisted Extraction ◽

Purification Process ◽

Microwave Assisted ◽

Assisted Extraction ◽

Moringa Oleifera Seed

Moringa seed has known as a coagulant in the water purification process. It is because of the large amount of anti-microbial compounds contained in it. Phenol is one of the most common anti-microbial compounds found in natural materials. The aim of this study was to determine the total phenolic content (TPC) of Moringa seed which was extracted by Microwave-Assisted Extraction (MAE). Moringa seeds were characterized by FTIR and showed that it contained phenol compounds confirmed by specific peak in some areas. Moringa seeds were characterized by FTIR before extracted. Therefore, MAE was performed by variation of solvent ratios (1: 4, 1: 6, 1: 8) and extraction time (2, 3, 4 min). The highest TPC of 41.78 mg GAE / g dw was reached at 1: 8 solvent ratio and 3-min extraction time.

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Optimization of Microwave-Assisted Extraction of Polysaccharides from Panax japlcus Var

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.774-776.919 ◽

2013 ◽

Vol 774-776 ◽

pp. 919-922

Author(s):

Xiao Song ◽

Peng Zhao

Keyword(s):

Microwave Power ◽

Extraction Yield ◽

Operating Conditions ◽

Raw Material ◽

Extraction Time ◽

Microwave Assisted Extraction ◽

Box Behnken Design ◽

Microwave Assisted ◽

Assisted Extraction ◽

Optimum Extraction

Polysaccharide fromPanax japlcus var(PJVPs) was extracted with microwave-assisted (MAE). The effects of operating conditions, such as extraction time, ratio of water to raw material , and microwave power, on the extraction yield of PJVPs were studied through a Box-Behnken design (BBD). The optimum extraction conditions were as follows: extraction time 9.14 min, power 728.5 W and ratio of water to raw material 27.57ml/g. The yield of polysaccharide was 14.02 % based on the above mentioned conditions.

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Ekstraksi Senyawa Fenolik Daun Kenikir (Cosmos caudatus) menggunakan Microwave Assisted Extraction (MAE)

Rona Teknik Pertanian ◽

10.17969/rtp.v11i1.9580 ◽

2018 ◽

Vol 11 (1) ◽

pp. 59-70

Author(s):

Angky Wahyu Putranto ◽

Shinta Rosalia Dewi ◽

Ni'matul Izza ◽

Dian Rahmat Yuneri ◽

Maria Yeniaska S. Dachi ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Extraction Time ◽

Total Phenolic ◽

Solvent Ratio ◽

Microwave Assisted Extraction ◽

Total Phenolic Compounds ◽

Microwave Assisted ◽

Cosmos Caudatus ◽

Assisted Extraction

Abstrak. Kenikir (Cosmos caudatus) adalah tanaman yang sering dimanfaatkan daunnya untuk bahan pangan maupun juga sebagai obat-obatan karena memiliki kandungan senyawa fenolik yang cukup tinggi. Ekstraksi senyawa fenol menggunakan metode konvensional masih memerlukan waktu yang cukup lama dan jumlah pelarut yang banyak. Sehingga perlu dicari alternatif metode ekstraksi yang memiliki waktu ekstraksi dan kebutuhan pelarut yang relatif rendah seperti menggunakan Microwave Assisted Extraction (MAE). Tujuan dari penelitian ini adalah melakukan ekstraksi senyawa fenolik dari daun kenikir dengan metode MAE terhadap total fenol dan aktivitas antioksidan. Penelitian ini menggunakan 2 faktor perlakuan yaitu rasio pelarut terhadap kenikir segar yaitu 1:4, 1:6, dan 1:8 (b/v) dan waktu ekstraksi 2, 3 dan 4 menit. Penelitian ini menjelaskan bahwa nilai total fenol tertinggi (17,162 ± 0,11 mg GAE/g berat segar) diperoleh pada perlakuan rasio pelarut 1:8 (b/v), sedangkan aktivitas antioksidan tertinggi (4,203 ± 0,26 mg/ml) pada perlakuan rasio pelarut 1:4 (b/v). Waktu perlakuan 3 menit menunjukkan hasil yang optimal untuk nilai total fenol dan aktivitas antioksidan. Hasil tersebut juga menunjukkan nilai yang lebih baik jika dibandingkan dengan metode konvensional baik dengan maserasi biasa dan maserasi dengan waterbath. Extraction of Phenolic Compounds from Cosmos caudatus using Microwave Assisted Extraction (MAE) Abstract. Cosmos caudatus, a plant commonly used its leaves for food and also as a medicine since it contains high phenolic compounds. The extraction of phenolic compounds using conventional methods still require more extraction time and need much more of solvent. Therefore, it was necessary to find an alternative extraction method that has a rapid extraction time and a few solvent need such as using Microwave Assisted Extraction (MAE). The aim of this study is to extract of phenolic compounds from Cosmos caudatus with MAE method of total phenol and antioxidant activity. This study using 2 treatment factors, the sampel-solvent ratio of 1:4, 1:6, and 1:8 (w/v) and the extraction time of 2, 3 and 4 minutes. This study explained that the highest total phenolic compounds (17.162 ± 0.11 mg GAE/g fw) and antioxidant activity (4.203 ± 0.26 mg/ml) were obtained at 1:8 and 1:4 (w/v) of sampel-solvent ratio, respectively. The extraction time of 3 minutes was the optimal condition for total phenolic compounds and antioxidant activity. These results also showed the highest parameter value when compared with both conventional methods: maceration and maceration with waterbath.

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Optimization of Microwave-Assisted Extraction of Polysaccharides from Acanthopanax Obouatus Hoo

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.549.504 ◽

2012 ◽

Vol 549 ◽

pp. 504-507

Author(s):

Xiao Rong Li ◽

Peng Zhao

Keyword(s):

Microwave Power ◽

Extraction Yield ◽

Operating Conditions ◽

Raw Material ◽

Extraction Time ◽

Microwave Assisted Extraction ◽

Box Behnken Design ◽

Microwave Assisted ◽

Assisted Extraction ◽

Optimum Extraction

Polysaccharide from Acanthopanax obouatus Hoo(AHPs) was extracted with microwave-assisted(MAE). The effects of operating conditions, such as extraction time, ratio of water to raw material , and microwave power, on the extraction yield of AHPs were studied through a Box-Behnken design (BBD). The optimum extraction conditions were as follows: extraction time 13.5min, power 796 W and ratio of water to raw material 16.5ml/g. The yield of polysaccharide was 5.18% based on the above mentioned conditions.

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Optimization of Microwave-Assisted Extraction (MAE) Time and Material to Solvent Ratio of Gembili (Dioscorea esculenta) Water-Soluble Polysaccharides (WSP)

Industria Jurnal Teknologi dan Manajemen Agroindustri ◽

10.21776/ub.industria.2021.010.01.9 ◽

2021 ◽

Vol 10 (1) ◽

pp. 78-98

Author(s):

Widya Dwi Rukmi Putri ◽

◽

Eko Isro Riyanto ◽

Almaria Heliana ◽

Ambo Wellang ◽

...

Keyword(s):

Water Soluble ◽

Extraction Time ◽

Solvent Ratio ◽

Microwave Assisted Extraction ◽

Optimum Conditions ◽

Design Expert ◽

Microwave Assisted ◽

Assisted Extraction ◽

Soluble Polysaccharide ◽

Central Composite

Abstract Gembili (Dioscorea esculenta) is a tuber-producing plant containing 14.63% inulin, a Water-Soluble Polysaccharide (WSP). This study aims to determine the optimum point of material to solvent ratio and Microwave-Assisted Extraction (MAE) time needed to extract gembili WSP, and determine the WSP's characteristics produced. The optimization process uses the Response Surface Methodology-Central Composite Design (RSM-CCD) with Design Expert 7.0 software. The material to solvent ratio's minimum and maximum points are 1:25 g/L and 1:45 g/L, while the minimum and maximum points of extraction time are 20 minutes and 40 minutes. This study observed 13 experimental combinations and responses from gembili WSP yield, WSP powder inulin levels, and WSP solubility. The results showed that the optimum conditions of material to solvent were at a 1:33.81 g/mL ratio, and the extraction time was 29.26 minutes. This optimum condition resulted in 32.42% WSP yield, 40.8% inulin content in WSP powder, 26.98% WSP solubility, 12.24% WSP water content, and 49.3 cp WSP viscosity. These results indicate that extraction using a microwave can increase WSP's rich inulin yield from gembili tubers. Keywords: gembili, inulin, microwave, optimization, Water-Soluble Polysaccharides

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Identification and Quantification of β-Sitosterol β-d-Glucoside of an Ethanolic Extract Obtained by Microwave-Assisted Extraction from Agave angustifolia Haw

Molecules ◽

10.3390/molecules24213926 ◽

2019 ◽

Vol 24 (21) ◽

pp. 3926

Author(s):

Herminia López-Salazar ◽

Brenda Hildeliza Camacho-Díaz ◽

Sandra Victoria Ávila-Reyes ◽

Ma Dolores Pérez-García ◽

Manases González- Cortazar ◽

...

Keyword(s):

High Performance ◽

Ethanolic Extract ◽

Dry Weight ◽

Extraction Time ◽

Ionization Mass ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Agave Angustifolia ◽

Assisted Extraction ◽

Ft Ir

β-sitosterol β-d-glucoside (BSSG) was extracted from “piña” of the Agave angustifolia Haw plant by microwave-assisted extraction (MAE) with a KOH solution such as a catalyst and a conventional maceration method to determine the best technique in terms of yield, extraction time, and recovery. The quantification and characterization of BSSG were done by high-performance thin layer chromatography (HPTLC), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography−electrospray ionization−mass spectrometry (HPLC-ESI-MS). With an extraction time of 5 s by MAE, a higher amount of BSSG (124.76 mg of β-sitosterol β-d-glucoside/g dry weight of the extract) than those for MAE extraction times of 10 and 15 s (106.19 and 103.97 mg/g dry weight respectively) was shown. The quantification of BSSG in the extract obtained by 48 h of conventional maceration was about 4–5 times less (26.67 mg/g dry weight of the extract) than the yields reached by the MAE treatments. MAE achieved the highest amount of BSSG, in the shortest extraction time while preserving the integrity of the compound’s structure.

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Evaluation of Ultrasound, Microwave, Ultrasound–Microwave, Hydrothermal and High Pressure Assisted Extraction Technologies for the Recovery of Phytochemicals and Antioxidants from Brown Macroalgae

Marine Drugs ◽

10.3390/md19060309 ◽

2021 ◽

Vol 19 (6) ◽

pp. 309

Author(s):

Marco Garcia-Vaquero ◽

Rajeev Ravindran ◽

Orla Walsh ◽

John O’Doherty ◽

Amit K. Jaiswal ◽

...

Keyword(s):

High Pressure ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Extraction Time ◽

Total Phenolic ◽

Solvent Ratio ◽

Microwave Assisted Extraction ◽

Novel Technologies ◽

Microwave Assisted ◽

Assisted Extraction

This study aims to explore novel extraction technologies (ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE), ultrasound–microwave-assisted extraction (UMAE), hydrothermal-assisted extraction (HAE) and high-pressure-assisted extraction (HPAE)) and extraction time post-treatment (0 and 24 h) for the recovery of phytochemicals and associated antioxidant properties from Fucus vesiculosus and Pelvetia canaliculata. When using fixed extraction conditions (solvent: 50% ethanol; extraction time: 10 min; algae/solvent ratio: 1/10) for all the novel technologies, UAE generated extracts with the highest phytochemical contents from both macroalgae. The highest yields of compounds extracted from F. vesiculosus using UAE were: total phenolic content (445.0 ± 4.6 mg gallic acid equivalents/g), total phlorotannin content (362.9 ± 3.7 mg phloroglucinol equivalents/g), total flavonoid content (286.3 ± 7.8 mg quercetin equivalents/g) and total tannin content (189.1 ± 4.4 mg catechin equivalents/g). In the case of the antioxidant activities, the highest DPPH activities were achieved by UAE and UMAE from both macroalgae, while no clear pattern was recorded in the case of FRAP activities. The highest DPPH scavenging activities (112.5 ± 0.7 mg trolox equivalents/g) and FRAP activities (284.8 ± 2.2 mg trolox equivalents/g) were achieved from F. vesiculosus. Following the extraction treatment, an additional storage post-extraction (24 h) did not improve the yields of phytochemicals or antioxidant properties of the extracts.

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