scholarly journals Atmospheric Pressure Plasma Irradiation Facilitates Transdermal Permeability of Aniline Blue on Porcine Skin and the Cellular Permeability of Keratinocytes with the Production of Nitric Oxide

2021 ◽  
Vol 11 (5) ◽  
pp. 2390
Author(s):  
Sunmi Lee ◽  
Jongbong Choi ◽  
Junghyun Kim ◽  
Yongwoo Jang ◽  
Tae Ho Lim
Keyword(s):  
Nitric Oxide ◽  
Molecular Weight ◽  
Transdermal Delivery ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Porcine Skin ◽  
Plasma Irradiation ◽  
Transdermal Absorption ◽  
Cellular Permeability ◽  
Transdermal Permeability

The transdermal delivery system of nutrients, cosmetics, and drugs is particularly attractive for painless, noninvasive delivery and sustainable release. Recently, atmospheric pressure plasma techniques have been of great interest to improve the drug absorption rate in transdermal delivery. Currently, plasma-mediated changes in the lipid composition of the stratum corneum are considered a possible mechanism to increase transdermal permeability. Nevertheless, its molecular and cellular mechanisms in transdermal delivery have been largely confined and still veiled. Herein, we present the effects of cold plasma on transdermal transmission on porcine skin and the cellular permeability of keratinocytes and further demonstrate the production of nitric oxide from keratinocytes. Consequently, argon plasma irradiation for 60 s resulted in 2.5-fold higher transdermal absorption of aniline blue dye on porcine skin compared to the nontreated control. In addition, the plasma-treated keratinocytes showed an increased transmission of high-molecular-weight molecules (70 and 150 kDa) with the production of nitric oxide. Therefore, these findings suggest a promoting effect of low-temperature plasma on transdermal absorption, even for high-molecular-weight molecules. Moreover, plasma-induced nitric oxide from keratinocytes is likely to regulate transdermal permeability in the epidermal layer.

scholarly journals Cold Atmospheric Pressure Plasma-Activated Medium Induces Selective Cell Death in Human Hepatocellular Carcinoma Cells Independently of Singlet Oxygen, Hydrogen Peroxide, Nitric Oxide and Nitrite/Nitrate

2021 ◽  
Vol 22 (11) ◽  
pp. 5548
Author(s):  
Yan Li ◽  
Tianyu Tang ◽  
Haejune Lee ◽  
Kiwon Song
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Singlet Oxygen ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Proliferative Effect ◽  
Huh7 Cells ◽  
Anti Cancer ◽  
Cold Atmospheric Pressure Plasma ◽  
Nitrite Nitrate

Cold atmospheric pressure plasma (CAP) and plasma-activated medium (PAM) induce cell death in diverse cancer cells and may function as powerful anti-cancer agents. The main components responsible for the selective anti-cancer effects of CAP and PAM remain elusive. CAP or PAM induces selective cell death in hepatocellular carcinoma cell lines Hep3B and Huh7 containing populations with cancer stem cell markers. Here, we investigated the major component(s) of CAP and PAM for mediating the selective anti-proliferative effect on Hep3B and Huh7 cells. The anti-proliferative effect of CAP was mediated through the medium; however, the reactive oxygen species scavenger N-acetyl cysteine did not suppress PAM-induced cell death. Neither high concentrations of nitrite or nitrite/nitrate nor a low concentration of H2O2 present in the PAM containing sodium pyruvate affected the viability of Hep3B and Huh7 cells. Inhibitors of singlet oxygen, superoxide anions, and nitric oxide retained the capacity of PAM to induce anti-cancer effects. The anti-cancer effect was largely blocked in the PAM prepared by placing an aluminum metal mesh, but not a dielectric PVC mesh, between the plasma source and the medium. Hence, singlet oxygen, hydrogen peroxide, nitric oxide, and nitrite/nitrate are not the main factors responsible for PAM-mediated selective death in Hep3B and Huh7 cells. Other factors, such as charged particles including various ions in CAP and PAM, may induce selective anti-cancer effects in certain cancer cells.

Effect of atmospheric-pressure plasma irradiation on the surface tension of water

2022 ◽  
Author(s):  
Naoki Shirai ◽  
Takuma Kaneko ◽  
Yuto Takamura ◽  
Koichi Sasaki
Keyword(s):  
Surface Tension ◽  
Gas Phase ◽  
Atmospheric Pressure ◽  
Water Surface ◽  
Atmospheric Pressure Plasma ◽  
Marangoni Effect ◽  
Oh Radicals ◽  
Plasma Irradiation ◽  
Pressure Plasma ◽  
Trapping Agent

Abstract We have shown that measuring the surface tension is a useful scheme to examine the plasma-liquid interface in real-time. The surface tension was measured using a method based on the dispersion relation of an acoustic capillary wave excited on the water surface. The surface tension gradually increased with time, when the water surface was irradiated with the outside region of the spatial afterglow of an atmospheric-pressure plasma. The Marangoni effect associated with the localized increase in the surface tension was observed during the plasma irradiation. The surface tension decreased after the termination of the discharge. A correlation was found between the transient decrease in the surface tension and the variation of the OH radical density in the gas phase. No increase in the surface tension was observed in the solution containing a trapping agent for liquid-phase OH radicals. These experimental results suggest that OH radicals act to increase the surface tension. However, the behavior of the surface tension cannot be explained perfectly by considering only the action of OH radicals.

Fat Liquefaction of Adipose Tissue Using Atmospheric-Pressure Plasma Irradiation

2011 ◽  
Vol 50 (8) ◽  
pp. 080217
Author(s):  
Takamichi Hirata ◽  
Chihiro Tsutsui ◽  
Takumi Kishimoto ◽  
Akira Mori ◽  
Masahiro Akiya ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Plasma Irradiation ◽  
Pressure Plasma

Fat Liquefaction of Adipose Tissue Using Atmospheric-Pressure Plasma Irradiation

2011 ◽  
Vol 50 (8R) ◽  
pp. 080217 ◽  
Author(s):  
Takamichi Hirata ◽  
Chihiro Tsutsui ◽  
Takumi Kishimoto ◽  
Akira Mori ◽  
Masahiro Akiya ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Plasma Irradiation ◽  
Pressure Plasma

Micro-Inactivation of Coliforms by Low-Temperature and Atmospheric-Pressure Plasma Irradiation

2015 ◽  
Vol 749 ◽  
pp. 74-78
Author(s):  
Hideyuki Takahashi ◽  
Satoshi Kurumi ◽  
Kaoru Suzuki ◽  
Katsushi Nishimura ◽  
Hideharu Hirose ◽  
...  
Keyword(s):  
Low Temperature ◽  
Emission Spectrum ◽  
Atmospheric Pressure ◽  
Oral Surgery ◽  
Irradiation Time ◽  
Atmospheric Pressure Plasma ◽  
Positive System ◽  
Plasma Irradiation ◽  
Pressure Plasma ◽  
Peak Intensity

In this study, we report on the inactivation technique to apply the oral surgery by micro low-temperature and atmospheric-pressure plasma irradiation utilizing a capillary nozzle. Emission spectrum of the micro-plasma showed OH and O radical, which were affected inactivation of coliform. Vibration temperature of the micro-plasma with 7998 K was calculated from peak-intensity at emission due to N22nd positive system. Inactivation region was larger than plasma irradiation spot, and it was increasing with plasma irradiation time.

Oxidation of nitric oxide by atmospheric pressure plasma in a resonant plasma reactor

2013 ◽  
Vol 46 (13) ◽  
pp. 135202 ◽  
Author(s):  
Takayoshi Yumii ◽  
Takashi Yoshida ◽  
Kyoji Doi ◽  
Noriaki Kimura ◽  
Satoshi Hamaguchi
Keyword(s):  
Nitric Oxide ◽  
Atmospheric Pressure ◽  
Plasma Reactor ◽  
Atmospheric Pressure Plasma ◽  
Pressure Plasma
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