scholarly journals Characterisation of the Material and Mechanical Properties of Atomic Force Microscope Cantilevers with a Plan-View Trapezoidal Geometry

2019 ◽  
Vol 9 (13) ◽  
pp. 2604 ◽  
Author(s):  
Ashley D. Slattery ◽  
Adam J. Blanch ◽  
Cameron J. Shearer ◽  
Andrew J. Stapleton ◽  
Renee V. Goreham ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscope ◽  
Young’S Modulus ◽  
Correction Factor ◽  
Young's Modulus ◽  
Spring Constant ◽  
Beam Equation ◽  
Euler Beam ◽  
Plan View ◽  
Atomic Force

Cantilever devices have found applications in numerous scientific fields and instruments, including the atomic force microscope (AFM), and as sensors to detect a wide range of chemical and biological species. The mechanical properties, in particular, the spring constant of these devices is crucial when quantifying adhesive forces, material properties of surfaces, and in determining deposited mass for sensing applications. A key component in the spring constant of a cantilever is the plan-view shape. In recent years, the trapezoidal plan-view shape has become available since it offers certain advantages to fast-scanning AFM and can improve sensor performance in fluid environments. Euler beam equations relating cantilever stiffness to the cantilever dimensions and Young’s modulus have been proven useful and are used extensively to model cantilever mechanical behaviour and calibrate the spring constant. In this work, we derive a simple correction factor to the Euler beam equation for a beam-shaped cantilever that is applicable to any cantilever with a trapezoidal plan-view shape. This correction factor is based upon previous analytical work and simplifies the application of the previous researchers formula. A correction factor to the spring constant of an AFM cantilever is also required to calculate the torque produced by the tip when it contacts the sample surface, which is also dependent on the plan-view shape. In this work, we also derive a simple expression for the torque for triangular plan-view shaped cantilevers and show that for the current generation of trapezoidal plan-view shaped AFM cantilevers, this will be a good approximation. We shall apply both these correction factors to determine Young’s modulus for a range of trapezoidal-shaped AFM cantilevers, which are specially designed for fast-scanning. These types of AFM probes are much smaller in size when compared to standard AFM probes. In the process of analysing the mechanical properties of these cantilevers, important insights are also gained into their spring constant calibration and dimensional factors that contribute to the variability in their spring constant.

Mechanical Properties of Poly 3C-SiC Thin Films According to Carrier Gas (H2) Concentration

2008 ◽  
Vol 600-603 ◽  
pp. 867-870
Author(s):  
Gwiy Sang Chung ◽  
Ki Bong Han
Keyword(s):  
Thin Film ◽  
Thin Films ◽  
Mechanical Properties ◽  
Surface Roughness ◽  
Atomic Force Microscope ◽  
Young’S Modulus ◽  
Young's Modulus ◽  
Carrier Gas ◽  
Nano Indentation ◽  
Atomic Force

This paper presents the mechanical properties of 3C-SiC thin film according to 0, 7, and 10 % carrier gas (H2) concentrations using Nano-Indentation. When carrier gas (H2) concentration was 10 %, it has been proved that the mechanical properties, Young’s Modulus and Hardness, of 3C-SiC are the best of them. In the case of 10 % carrier gas (H2) concentration, Young’s Modulus and Hardness were obtained as 367 GPa and 36 GPa, respectively. When the surface roughness according to carrier gas (H2) concentrations was investigated by AFM (atomic force microscope), when carrier gas (H2) concentration was 10 %, the roughness of 3C-SiC thin was 9.92 nm, which is also the best of them. Therefore, in order to apply poly 3C-SiC thin films to MEMS applications, carrier gas (H2) concentration’s rate should increase to obtain better mechanical properties and surface roughness.

scholarly journals Alteration of nanomechanical properties of pancreatic cancer cells through anticancer drug treatment revealed by atomic force microscopy

2021 ◽  
Vol 12 ◽  
pp. 1372-1379
Author(s):  
Xiaoteng Liang ◽  
Shuai Liu ◽  
Xiuchao Wang ◽  
Dan Xia ◽  
Qiang Li
Keyword(s):  
Mechanical Properties ◽  
Pancreatic Cancer ◽  
Atomic Force Microscopy ◽  
Cancer Cells ◽  
Young’S Modulus ◽  
Young's Modulus ◽  
Force Microscopy ◽  
Pancreatic Cancer Cells ◽  
Atomic Force ◽  
Aggressive Cancer

The mechanical properties of cells are key to the regulation of cell activity, and hence to the health level of organisms. Here, the morphology and mechanical properties of normal pancreatic cells (HDPE6-C7) and pancreatic cancer cells (AsPC-1, MIA PaCa-2, BxPC-3) were studied by atomic force microscopy. In addition, the mechanical properties of MIA PaCa-2 after treatment with different concentrations of doxorubicin hydrochloride (DOX) were also investigated. The results show the Young's modulus of normal cells is greater than that of three kinds of cancer cells. The Young's modulus of more aggressive cancer cell AsPC-1 is smaller than that of less aggressive cancer cell BxPC-3. In addition, the Young's modulus of MIA PaCa-2 rises with the increasing of DOX concentration. This study may provide a new strategy of detecting cancer, and evaluate the possible interaction of drugs on cells.

scholarly journals Atomic force microscopy in the assessment of erythrocyte membrane mechanical properties with exposure to various physicochemical agents

2021 ◽  
Vol 49 (6) ◽  
pp. 427-434
Author(s):  
E. A. Sherstyukova ◽  
V. A. Inozemtsev ◽  
A. P. Kozlov ◽  
O. E. Gudkova ◽  
V. A. Sergunova
Keyword(s):  
Mechanical Properties ◽  
Young’S Modulus ◽  
Erythrocyte Membrane ◽  
Uv Radiation ◽  
Structural Organization ◽  
Young's Modulus ◽  
Homogeneous Medium ◽  
Topological Defects ◽  
Erythrocyte Membranes ◽  
Atomic Force

Background: Mechanical properties of cell membranes and their structural organization are considered among the most important biological parameters affecting the functional state of the cell. Under the influence of various pathogenic factors, erythrocyte membranes lose their elasticity. The resulting changes in their biomechanical characteristics is an important, but poorly studied topic. It is of interest to study the deformation of native erythrocytes to a depth compatible with their deformation in the bloodstream.Aim: To investigate the patterns of deep deformation and the particulars of structural organization of native erythrocyte membranes before and after their exposure to physicochemical agents in vitro.Materials and methods: Cell morphology, nanostructure characteristics, and membrane deformation of native erythrocytes in a  solution of hemoconservative CPD/SAGM were studied with atomic force microscope NTEGRA Prima. Hemin, zinc ions (Zn2+), and ultraviolet (UV) radiation were used as modifiers. To characterize the membrane stiffness, we measured the force curves F(h), hHz (the depth to which the probe immersion is described by interaction with a homogeneous medium), and the Young's modulus values of the erythrocyte membrane.Results: Exposure to hemin, Zn2+ and UV radiation led to transformation of the cell shape, appearance of topological defects and changes in mechanical characteristics of erythrocyte membranes. Under exposure to hemin, Young's modulus increased from 10±4  kPa to 27.2±8.6  kPa (p<0.001), exposure to Zn2+, to 21.4±8.7  kPa (p=0.002), and UV, to 18.8±5.6  kPa (p=0.001). The hHz value was 815±210  nm for the control image and decreased under exposure to hemin to 420±80 nm (p<0.001), Zn2+, to 370±90 nm (p<0.001), and UV, to 614±120 nm (p=0.001).Conclusion: The results obtained contribute to a  deeper understanding of interaction between membrane surfaces of native erythrocytes and small vessel walls. They can be useful in clinical medicine as additional characteristics for assessment of the quality of packed red blood cells, as well as serve as a basis for biophysical studies into the mechanisms of action of oxidative processes of various origins.

Young’s Modulus Measurement of Electroplated Nickel Using AFM

2006 ◽  
Author(s):  
Sang-Hyun Kim ◽  
James G. Boyd
Keyword(s):  
Elastic Modulus ◽  
Young’S Modulus ◽  
Young's Modulus ◽  
Beam Theory ◽  
Spring Constant ◽  
Time Step ◽  
Simple Method ◽  
Atomic Force ◽  
Silicon Cantilever ◽  
Afm Cantilever

This paper addresses a relatively simple method of measuring Young's modulus of electroplated nickel using Atomic Force Microscope. Thin layer of nickel to be measured is electroplated onto the tip side of AFM silicon cantilever, whose Young's modulus and the geometric dimensions are defined from manufacturer. The resonant frequency and the quality factor of the electroplated AFM cantilever are measured by the tapping mode of AFM and its spring constant is calculated using Sader's method. The spring constant of the electroplated cantilever is also calculated by using the laminar composite beam theory. Comparing two spring constants, Young's modulus of the electroplated nickel is determined. The measured elastic modulus of nickel in each time step is in the range of between and the average elastic modulus is with relative uncertainty of less than 5%

scholarly journals Atomic Simulation of Nanoindentation on the Regular Wrinkled Graphene Sheet

Materials ◽  
2020 ◽  
Vol 13 (5) ◽  
pp. 1127
Author(s):  
Ruonan Wang ◽  
Haosheng Pang ◽  
Minglin Li ◽  
Lianfeng Lai
Keyword(s):  
Mechanical Properties ◽  
Boundary Condition ◽  
Boundary Conditions ◽  
Young’S Modulus ◽  
Young's Modulus ◽  
Md Simulations ◽  
Force Microscopy ◽  
Atomic Simulation ◽  
Atomic Force ◽  
Surface Wrinkling

Surface landscapes have vague impact on the mechanical properties of graphene. In this paper, single-layered graphene sheets (SLGS) with regular wrinkles were first constructed by applying shear deformation using molecular dynamics (MD) simulations and then indented to extract their mechanical properties. The influence of the boundary condition of SLGS were considered. The wrinkle features and wrinkle formation processes of SLGS were found to be significantly related to the boundary conditions as well as the applied shear displacement and velocity. The wrinkling amplitude and degree of wrinkling increased with the increase in the applied shear displacements, and the trends of wrinkling wavelengths changed with the different boundary conditions. With the fixed boundary condition, the degree of graphene wrinkling was only affected when the velocity was greater than a certain value. The effect of wrinkles on the mechanical characterization of SLGS by atomic force microscopy (AFM) nanoindentation was finally investigated. The regular surface wrinkling of SLGS was found to weaken the Young’s modulus of graphene. The Young’s modulus of graphene deteriorates with the increase in the degree of regular wrinkling.

Microscopic Methods for Characterization of Selected Surface Properties of Biodegradable, Nanofibrous Tissue Engineering Scaffolds

2017 ◽  
Vol 890 ◽  
pp. 213-216 ◽  
Author(s):  
Adrian Chlanda ◽  
Ewa Kijeńska ◽  
Wojciech Święszkowski
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Young’S Modulus ◽  
Young's Modulus ◽  
Force Spectroscopy ◽  
Operating Mode ◽  
Tissue Engineering Scaffolds ◽  
Force Microscopy ◽  
Atomic Force

Biodegradable polymeric fibers with nanoand submicron diameters, produced by electrospinning can be used as scaffolds in tissue engineering. It is necessary to characterize their mechanical properties especially at the nanoscale. The Force Spectroscopy is suitable atomic force microscopy mode, which allows to probe mechanical properties of the material, such as: reduced Young's modulus, deformation, adhesion, and dissipation. If combined with standard operating mode: contact or semicontact, it will also provide advanced topographical analysis. In this paper we are presenting results of Force Spectroscopy characterization of two kinds of electrospun fibers: polycaprolactone and polycaprolactone with hydroxyapatite addition. The average calculated from Johnson-Kendall-Roberts theory Young's modulus was 4 ± 1 MPa for pure polymer mesh and 20 ± 3 MPa for composite mesh.

Microstructure and Mechanical Properties of TiN/NbN Multilayered Coating

2010 ◽  
Vol 434-435 ◽  
pp. 466-468
Author(s):  
Chien Cheng Liu ◽  
Kuang I Liu ◽  
Huai Wei Yan ◽  
Chia Li Ma ◽  
Jow Lay Huang
Keyword(s):  
Mechanical Properties ◽  
Young’S Modulus ◽  
Young's Modulus ◽  
Steel Ball ◽  
Force Microscopy ◽  
Atomic Force ◽  
Pin On Disk ◽  
Heating Up ◽  
Steel Substrates ◽  
Stylus Profiler

In this study, multilayers of TiN/NbN were deposited by d.c. magnetron sputtering on die steel substrates. The structure, morphology and nano-hardness were assessed using X-ray diffraction, atomic force microscopy (AFM), stylus profiler (XP-2 stylus profiler) and nanoindentation, respectively. Wear tests were performed on pin-on-disk configuration and dry sliding conditions, at 5N load by using hardened steel ball. The result shows TiN with highly (111) preferred orientation. On mechanical properties, Young’s modulus and hardness values increase for layers number increase. At 64 layers films had the highest nano-hardness, Young’s modulus values. The TiN/NbN multilayer films presented changes in its morphology becoming more granulated and density after heating up to 500°C. A significant decrease in friction coefficient has been achieved for TiN/NbN multilayers against steel ball.

Evaluating Young's Modulus of Single Yeast Cells Based on Compression Using an Atomic Force Microscope with a Flat Tip

2021 ◽  
pp. 1-8
Author(s):  
Di Chang ◽  
Takahiro Hirate ◽  
Chihiro Uehara ◽  
Hisataka Maruyama ◽  
Nobuyuki Uozumi ◽  
...  
Keyword(s):  
Atomic Force Microscope ◽  
Young’S Modulus ◽  
Young's Modulus ◽  
Yeast Cells ◽  
Atomic Force

Abstract

scholarly journals Тестирование на изгиб наноразмерных консолей в атомно-силовом микроскопе

2022 ◽  
Vol 48 (3) ◽  
pp. 24
Author(s):  
А.В. Анкудинов ◽  
М.М. Халисов
Keyword(s):  
Atomic Force Microscope ◽  
Young’S Modulus ◽  
Elastic Foundation ◽  
Test Data ◽  
Young's Modulus ◽  
Atomic Force ◽  
Good Agreement

Consoles and bridges of MgNi2Si2O5(OH)4 nanoscrolls were tested for bending in atomic force microscope. Using test data, we analyze how the consoles or bridges were fixed, and took this information into account when calculating the Young's modulus of the nanoscrolls. The results on the consoles are in good agreement with the results on the bridges when modeling the latter as three-span beams, and the former as beams on an elastic foundation with a suspended console.

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