scholarly journals Effects of an Acetic Acid and Acetone Mixture on the Characteristics and Scaffold–Cell Interaction of Electrospun Polycaprolactone Membranes

2019 ◽  
Vol 9 (20) ◽  
pp. 4350 ◽  
Author(s):  
Minh Hieu Ho ◽  
Thien Bui-Thuan Do ◽  
Nhi Ngoc-Thao Dang ◽  
An Nguyen-My Le ◽  
Hanh Thi-Kieu Ta ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Cell Interaction ◽  
Fibrous Materials ◽  
Cell Adherence ◽  
Physical Strength ◽  
Membrane Morphology ◽  
Cell Scaffold ◽  
Different Types ◽  
Proliferation Assays ◽  
Cell Adhesion And Proliferation

Green electrospinning has attracted great interest since non-toxic solvents were shown to be applicable in the fabrication of fibrous materials while ensuring health safety and environmental protection. Less harmful reagents such as acetone (AC) and acetic acid (AA) have been employed in this field in recent years. However, research in this area is still rare, yielding only preliminary results. In this study, two different types of solvents (pure AC and an AA/AC mixture) were used to fabricate electrospun polycaprolactone (PCL) membranes. Sample morphology, wettability, tensile strength, and chemical composition were compared between two types of membranes. Cell–scaffold interaction was also examined by cell adhesion and proliferation assays. The results demonstrate that the two types of solvents had significant effects on membrane morphology, physical strength, and cell adherence behaviors, which should be considered for different application purposes.

Studies on Chemical Methods to Expose Gate/Tunnel Oxide and Identification of Gate/Tunnel Oxide Defects in Wafer Fabrication

2002 ◽  
Author(s):  
Y. N. Hua ◽  
G. B. Ang ◽  
S. Redkar ◽  
Yogaspari ◽  
Wilma Richter
Keyword(s):  
Acetic Acid ◽  
Silicon Oxide ◽  
Wafer Fabrication ◽  
Chemical Methods ◽  
Advantages And Disadvantages ◽  
Different Types ◽  
Capacitor Structure ◽  
Oxide Defects ◽  
Buffer Oxide Etchant ◽  
Etch Time

Abstract In failure analysis of wafer fabrication, currently, three different types of chemical methods including 6:6:1 (Acetic Acid/HNO3/HF), NaOH and Choline are used in removing polysilicon (poly) layer and exposing the gate/tunnel oxide underneath. However, usage is limited due to their disadvantages. For example, 6:6:1 is a relatively fast etchant, but it is difficult to control the etch time and keep the oxide layer intact. Also, while using NaOH to remove poly and expose the silicon oxide, the solution needs to be heated. It is also difficult to etch a poly layer with a WSix or a CoSix silicide using NaOH. In this paper, we will discuss these 3 etchants in terms of their advantages and disadvantages. We will then introduce a new poly etchant, called HB91. HB91 is useful for removing poly to expose the gate/tunnel oxide for identification of related defects. HB91 is actually a mixture of two chemicals namely nitric acid (HNO3) and buffer oxide etchant (BOE) in a 9:1 ratio. The experimental results show that it is highly selective in poly removal with respect to the gate/tunnel oxide and is a suitable poly etchant especially for removing polysilicon with/without WSix and CoSix in the large capacitor structure. Application results of this poly etchant (HB91) will be presented.

Mesenchymal Stem Cells and Cancer Stem Cells: An Overview of Tumor-Mesenchymal Stem Cell Interaction for therapeutic interventions

2021 ◽  
Vol 22 ◽  
Author(s):  
Soheila Montazersaheb ◽  
Ezzatollah Fathi ◽  
Ayoub Mamandi ◽  
Raheleh Farahzadi ◽  
Hamid Reza Heidari
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Tumor Microenvironment ◽  
Cancer Stem Cells ◽  
Tumor Cells ◽  
Cell Types ◽  
Cell Interaction ◽  
Therapeutic Interventions ◽  
Tumorigenic Potential ◽  
Different Types

: Tumors are made up of different types of cancer cells that contribute to tumor heterogeneity. Among these cells, cancer stem cells (CSCs) have a significant role in the onset of cancer and development. Like other stem cells, CSCs are characterized by the capacity for differentiation and self-renewal. A specific population of CSCs is constituted by mesenchymal stem cells (MSCs) that differentiate into mesoderm-specific cells. The pro-or anti-tumorigenic potential of MSCs on the proliferation and development of tumor cells has been reported as contradictory results. Also, tumor progression is specified by the corresponding tumor cells like the tumor microenvironment. The tumor microenvironment consists of a network of reciprocal cell types such as endothelial cells, immune cells, MSCs, and fibroblasts as well as growth factors, chemokines, and cytokines. In this review, recent findings related to the tumor microenvironment and associated cell populations, homing of MSCs to tumor sites, and interaction of MSCs with tumor cells will be discussed.

scholarly journals Avian neural crest cell attachment to laminin: involvement of divalent cation dependent and independent integrins

Development ◽  
1991 ◽  
Vol 113 (4) ◽  
pp. 1069-1084 ◽  
Author(s):  
T. Lallier ◽  
M. Bronner-Fraser
Keyword(s):  
Neural Crest ◽  
Cell Attachment ◽  
Divalent Cations ◽  
Neural Crest Cell ◽  
Neural Crest Cells ◽  
Cell Interaction ◽  
Cell Binding ◽  
Cell Adherence ◽  
Beta 1 Integrin ◽  
Crest Cell

The mechanisms of neural crest cell interaction with laminin were explored using a quantitative cell attachment assay. With increasing substratum concentrations, an increasing percentage of neural crest cells adhere to laminin. Cell adhesion at all substratum concentrations was inhibited by the CSAT antibody, which recognizes the chick beta 1 subunit of integrin, suggesting that beta 1-integrins mediate neural crest cell interactions with laminin. The HNK-1 antibody, which recognizes a carbohydrate epitope, inhibited neural crest cell attachment to laminin at low coating concentrations (greater than 1 microgram ml-1; Low-LM), but not at high coating concentration of laminin (10 micrograms ml-1; High-LM). Attachment to Low-LM occurred in the absence of divalent cations, whereas attachment to High-LM required greater than 0.1 mM Ca2+ or Mn2+. Neural crest cell adherence to the E8 fragment of laminin, derived from its long arm, was similar to that on intact laminin at high and low coating concentrations, suggesting that this fragment contains the neural crest cell binding site(s). The HNK-1 antibody recognizes a protein of 165,000 Mr which is also found in immunoprecipitates using antibodies against the beta 1 subunit of integrin and is likely to be an integrin alpha subunit or an integrin-associated protein. Our results suggest that the HNK-1 epitope on neural crest cells is present on or associated with a novel or differentially glycosylated form of beta 1-integrin, which recognizes laminin in the apparent absence of divalent cations. We conclude that neural crest cells have at least two functionally independent means of attachment to laminin which are revealed at different substratum concentrations and/or conformations of laminin.

Proliferation and Adhesion of L929 Fibroblasts on Surface with Different Microtopography

2014 ◽  
Vol 1648 ◽  
Author(s):  
Yuta Kurashina ◽  
Shogo Miyata ◽  
Jun Komotori ◽  
Tadayoshi Koyama
Keyword(s):  
Cell Proliferation ◽  
Cell Adhesion ◽  
Low Frequency ◽  
Polished Surface ◽  
Stainless Steel Surface ◽  
Cell Counts ◽  
Adhesion Behavior ◽  
Cell Scaffold ◽  
Cell Adhesion And Proliferation ◽  
Number Of Cells

ABSTRACTThree types of 316L stainless steel surface with different topography were prepared by a Fine Particle Peening (FPP) treatment using titania, silica and alumina shot particles and analyzed the cell proliferation and cell-scaffold interaction. FPP-treated surface with titania and silica particles had micro asperities at low frequency. On the other hand, the alumina treated surface had micro asperities at high frequency. L929 fibroblasts were seeded on these specimens and then the number of cells was counted after 72 hours of culturing. The FPP-treated surfaces showed good cell proliferation comparing to polished surface. This indicates that micro asperities formed on the surface encourage cell adhesion. Cell adhesion behavior was evaluated by a scanning electron microscope (SEM) and a fluorescence microscope. Dense filopodia were observed when cells cultured on the FPP-treated surface. This means that FPP treatment enhances cell adhesion and proliferation. The number of cells observed on the FPP-treated surface depended on the shape of asperities formed by FPP treatment; the highest cell counts were obtained on alumina treated surface. This is because cell migration was not inhibited by the shape of alumina treated surface asperities.

Synthesis of Three Different Types of Chalcone Derivatives

2014 ◽  
Vol 1033-1034 ◽  
pp. 81-84
Author(s):  
He Ping Yan ◽  
Du Shu Huang ◽  
Ju Cheng Zhang ◽  
Rui Na Huang ◽  
You Wang Fu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Hydrochloric Acid ◽  
Room Temperature ◽  
Glacial Acetic Acid ◽  
Chalcone Derivatives ◽  
Different Types ◽  
Naoh Solution

Three chalcone derivatives were synthesized by the reaction of acetophenone with dimethylamino benzaldehyde, vanillin, diphenyl formaldehyde, respectively. The results showed that: A yield of 80.4% was obtained by the reaction of acetophenone and dimethylamino benzaldehyde in ethanol as solvent, 10% NaOH solution as catalyst at room temperature. A yield of 25.0% was obtained by the reaction of acetophenone and vanillin in ethanol as solvent, a mixed glacial acetic acid and hydrochloric acid as the catalyst, adding 10% KOH solution for dehydration by heating after the reaction at room temperature. A yield of 76.3% was obtained by the reaction of acetophenone and diphenyl formaldehyde in ethanol as solvent, 10% NaOH solution as catalyst at room temperature. Acid was used as the appropriate catalyst when chalcone derivatives were synthesized by aldehyde with hydroxy.

Magnetic nanoparticles: mechanistic studies on the cancer cell interaction

2016 ◽  
Vol 5 (5) ◽  
Author(s):  
Joe Antony Jacob ◽  
Jumah Masoud Mohammad Salmani ◽  
Baoan Chen
Keyword(s):  
Cell Death ◽  
Magnetic Nanoparticles ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Cell Interaction ◽  
Mechanistic Studies ◽  
Normal Cells ◽  
Different Types ◽  
Induction Of Apoptosis ◽  
Plausible Reason

AbstractMagnetic nanoparticles are renowned for their anticancer activity. Recent studies have elucidated that magnetic nanoparticles induce cytotoxicity by induction of apoptosis in cancer cells. The magnetic nanoparticles can also be biosynthesized, and this presents an added advantage along with the concept of limited toxicity to normal cells. This review focuses on the mechanistic studies performed on the anticancer activity of different types of magnetic nanoparticles. Apoptosis was shown to be the most plausible reason behind the cell death mediated by various types of magnetic nanoparticles.

scholarly journals ASPECTS OF VINEGARS PRODUCTION AND MARKETING IN MOLDOVA

2021 ◽  
Vol IV (2) ◽  
pp. 128-138
Author(s):  
Alina Boistean ◽  
Keyword(s):  
Acetic Acid ◽  
Raw Materials ◽  
Production Method ◽  
Raw Material ◽  
Local Market ◽  
Tropical Fruit ◽  
Current Trends ◽  
The World ◽  
Different Types ◽  
Balsamic Vinegar

Vinegar is one of the oldest fermented products in the world and its production dates back to around 2000 BC. It is a liquid fermentation product that consists from 4% to 9% acetic acid, which is commonly used and consumed globally, either directly or as a condiment in food. It is produced by two-stage fermentation systems, i.e., the alcoholic fermentation of sugary substrates and subsequently, the acetous fermentation (oxidation) of the ethanol into acetic acid. There are numerous different types of vinegars produced worldwide based on the raw material and the production method used. Among all the vinegars available, the most common types of vinegar, produced in different parts of the world, and including some top-quality famous products, are “cider vinegar,” “balsamic vinegar,” “wine vinegar,” “rice vinegar,” and “malt vinegar.” Tropical fruit vinegars and vinegars made from any kind of agricultural surpluses are also available. The different raw materials and the methods used for the production of the different types of vinegars lead to a variety of products with distinctive characteristics. In this paper, definitions, local market and legislation and current trends in vinegar production are briefly highlighted and discussed.

scholarly journals Effect of short-term temperature stresses on the level of indole-3-acetic acid in plants with different types of ecological strategy

2013 ◽  
Vol 70 (2) ◽  
pp. 264-269
Author(s):  
I.V. Kosakivska ◽  
◽  
L.V. Voytenko ◽  
R.V. Lichnevskiy ◽  
A.Yu. Ustinova ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Ecological Strategy ◽  
Short Term ◽  
Temperature Stresses ◽  
Different Types ◽  
Indole 3 Acetic Acid

scholarly journals The influence of whey, whey component and malt on the growth and acids production of lactobacilli in milk

2014 ◽  
Vol 32 (No. 6) ◽  
pp. 526-531 ◽  
Author(s):  
Š. Horáčková ◽  
P. Sedláčková ◽  
M. Sluková ◽  
M. Plocková
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Whey Protein ◽  
Lactobacillus Acidophilus ◽  
Acid Production ◽  
Lactobacillus Casei ◽  
Whey Protein Concentrate ◽  
Protein Concentrate ◽  
Different Types ◽  
Plant Sources

The effect of whey powder, whey protein concentrate, caseinomacropeptide, and malt addition into milk on the growth and acid production of lactobacilli (Lactobacillus casei Lafti L-26, Lactobacillus acidophilus CCDM 151, and Lactobacillus casei CCDM 198) was evaluated. The ability of these strains to use different types of saccharides from milk and plant sources was also tested. Glucose, galactose, fructose and maltose were utilised by all tested strains. The results showed that the addition of malt positively affected the growth of lactobacilli strains compared to the growth in milk enriched by whey ingredients. The addition of malt increased significantly the production of d(–)isomer of lactic acid by Lactobacillus acidophilus CCDM 151 and Lactobacillus casei CCDM 198 and the production of acetic acid by Lactobacillus casei CCDM 198.  

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