scholarly journals Changes in Anthocyanin and Antioxidant Contents during Maturation of Australian Highbush Blueberry (Vaccinium corymbosum L.) Cultivars

2021 ◽  
Vol 11 (1) ◽  
pp. 6
Author(s):  
Joel B. Johnson ◽  
Michelle Steicke ◽  
Janice S. Mani ◽  
Shiwangni Rao ◽  
Scott Anderson ◽  
...  

The Australian blueberry industry is worth over $300 million, but there is limited information on factors influencing their chemical composition, particularly their ripeness and harvest stage. This pilot study investigated changes in total monomeric anthocyanin content (TMAC; measured using the pH-differential method) and total antioxidant capacity (TAC; measured with the cupric reducing antioxidant capacity assay) of four Australian highbush blueberry cultivars (Denise, Blue Rose, Brigitta and Bluecrop) at four time points and three maturity stages (unripe, moderately ripe and fully ripe). The TAC of most cultivars decreased by 8–18% during ripening, although that of the Blue Rose cultivar increased markedly. However, the TAC of ripe fruit from this cultivar also fluctuated markedly throughout the harvest season (between 1168–2171 mg Trolox equivalents 100 g−1). The TMAC increased sharply between the medium-ripe and fully ripe maturity stages, with the Blue Rose cultivar showing the highest TMAC values (211 mg 100 g−1, compared to 107–143 mg 100 g−1 for the remaining varieties). The TMAC of ripe fruit from this cultivar also rose steadily throughout the harvest season, in contrast to most other cultivars where the TMAC fell slightly over time. These results indicate that the levels of health-benefitting compounds in Australian-grown highbush blueberries may depend not only on the cultivar, but also upon the time of harvest.

Author(s):  
JÚLIA RIBEIRO SARKIS ◽  
ISABEL CRISTINA TESSARO ◽  
LIGIA DAMASCENO FERREIRA MAECZAK

The objective of this study was to apply a HPLC methodology for qualitative and quantitative analysis of the six anthocyanidins present in fruits and to analyze the anthocyanin and anthocyanidin content of blueberries cultivated in Southern Brazil. The samples used belong to highbush blueberries (Vaccinium corymbosum) cultivars. Total anthocyanin content was determined by the pH differential method and an HPLC gradient elution system with C18 column and UV-Vis detection at 520 nm were used for separation and quantification of the anthocyanidins. Total anthocyanin content was of 128 ± 3 mg per 100 g of fresh pulp. Blueberry pulp presented 55 % of delphinidin, 8 % of cyanidin, 3 % of peonidin and 34 % of malvidin. Pelargonidin was not identified in the sample and petunidin was below the limits of quantification. The results were similar to those reported in studies using North American and European blueberries.


HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 394C-394
Author(s):  
W. Kalt ◽  
C. Lawand ◽  
C.F. Forney

Highbush blueberry (Vaccinium corymbosum) fruit of the cultivars `Bergitta', `Bluegold', and `Nelson' were harvested at six stages of maturity and evaluated for their antioxidant capacity and anthocyanin and phenolic content. Fruit of the four earliest maturities were also stored at 20 °C for up to 8 days. At the time of harvest, fruit of different maturities had substantial differences in their anthocyanin content, and less marked differences in phenolic content and antioxidant capacity. Substantial anthocyanin synthesis occurred in under-ripe fruit during 20 °C storage, and varied depending on fruit maturity at harvest. Total phenolic content changed very little during storage, and there was no change in fruit antioxidant capacity. The results suggest that anthocyanin phenolics are formed on or off the plant, primarily from other pre-existing phenolic components. Whether phenolics are present as anthocyanins or other colorless forms, has relatively little impact on antioxidant capacity.


2003 ◽  
Vol 128 (6) ◽  
pp. 917-923 ◽  
Author(s):  
Wilhelmina Kalt ◽  
Christopher Lawand ◽  
Daniel A.J. Ryan ◽  
Jane E. McDonald ◽  
Horst Donner ◽  
...  

The antioxidant properties of blueberries have been examined only in ripe fruit, although fruit of different maturities are used in processed food products. In this study, highbush blueberry cultivars Bergitta, Bluegold, and Nelson highbush blueberry fruit at different stages of ripeness were examined to characterize differences in oxygen radical absorbing capacity (ORAC) and the phenolic components responsible for ORAC. Underripe fruit at different stages of maturity were also stored at 20 °C for up to 8 days to assess changes in ORAC and phenolic content. Anthocyanin content was substantially higher in fruit of more advanced stages of ripeness. In contrast, the phenolic content and ORAC were lower in the riper fruit. Anthocyanins continued to form during storage, although rate of pigment formation declined after about 4 days. Less anthocyanin pigment was formed in the less ripe fruit. After 8 days of storage, the anthocyanin content of fruit harvested 5% to 50% or 50% to 95% blue exceeded that of ripe fruit. Up to 60% of the total phenolic content could be accounted for by anthocyanins. ORAC was positively correlated with total phenolic content (R2 = 0.78), but not with anthocyanin content.


Author(s):  
Mariana Atena Poiană ◽  
I. Gergen ◽  
Diana Moigrădean ◽  
Viorica Târu ◽  
Diana Dogaru

In this paper it was obtained the apple vinegar with addition of red wines concentrates in different percents for to improve the antioxidant properties. For processing of red wine concentrates it was used the young red wines Merlot and Cabernet Sauvignon. For resulted vinegar types were analyzed total acidity, extract, total antioxidant capacity (using FRAP method), total polyphenols amount (by Folin-Ciocalteu method) and monomeric anthocyanins (using pH-differential method). Polyphenols content from vinegar with Cabernet Sauvignon concentrates addition was situated in the range 0.74-3.42 mM gallic acid/L and for vinegar with red wine Merlot concentrates addition between 0.74-2.64 mM gallic acid/L. The antioxidant capacity was presented the values between 0.45-8.18 mM Fe2+/L for apple vinegar with Cabernet Sauvignon concentrates addition and between 0.45-6.69 mM Fe2+/L for vinegar with Merlot concentrate addition. The polyphenols content and monomeric anthocyanins content of apple vinegars with red wine concentrates increase in rapport with the percent of red wines concentrates added. The values of polyphenols content and total antioxidant capacity were more with approximate 20% in the case of vinegar with Cabernet Sauvignon concentrates addition comparatively with the case of vinegar with Merlot concentrates addition.


2012 ◽  
Vol 58 (No. 5) ◽  
pp. 236-241 ◽  
Author(s):  
S. Mihaljević ◽  
B. Salopek-Sondi

Auxins and their synthetic analogues are commonly used for rooting of cuttings, but their efficiency depends on experimental set-up and, even more importantly, on species or cultivar, and type of explants investigated. In attempt to improve rooting procedure for highbush blueberries (Vaccinium corymbosum L.), we investigated alanine conjugate of indole-3-butyric acid (IBA-Ala) as potential root-promoting compound and compared with commonly used auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). The effect of different concentrations of auxins on the rooting of highbush blueberry stem cuttings (var. Bluecrop, Bluetta, Burlington and Jersey) and in vitro-derived microcuttings (var. Jersey) was investigated. Auxin treatments significantly promoted rooting of all four varieties in comparison to control. The most efficient rooting promoter in all varieties appeared IBA-Ala (provided up to 83%, and 93% of the rooted cuttings and microcuttings, respectively). Furthermore, IBA-Ala caused a vigorous, well-branched root system that resulted in better acclimatization and survival of plants. Results suggest the application of IBA-Ala may be beneficial for rooting of difficult-to-root varieties of blueberry and related species and their practical application in the nursery industry.  


HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 582a-582
Author(s):  
Jane Beggs

Deformed or damaged berries reduce the grade of frozen highbush blueberries (Vaccinium corymbosum L.). Before berries are frozen, immature fruit are commonly removed by density sorting in water tanks. Three studies were conducted to determine if the firmness or quality of highbush blueberries could be improved by the addition of CaCl2 to sorting tank water. `Bluecrop' and `Jersey' berries were dipped in CaCl2 solutions (0.0-4.0%) for periods of 0.5-8.0 minutes. The effect of rinsing the fruit after treatment was also studied. Berries were held at 2C for several days before evaluation. A 61.5 cm length of PVC pipe (4.0 cm. I.D.) was filled to a depth of 50 cm. with berries and dropped 4 times on to a hard surface from a height of 10 cm. The compression of the column of berries was measured and berries were removed and visually sorted according to the degree of damage. The amount of compression and number of damaged berries were inversely related to the CaCl2 concentration. Rinsing berries immediately after dips negated the effects of CaCl2. Treatment with CaCl2 may result in objectionable flavors.


HortScience ◽  
1993 ◽  
Vol 28 (1) ◽  
pp. 33-35 ◽  
Author(s):  
C.B. Ely ◽  
R.E. Frans ◽  
T.L. Lavy ◽  
R.E. Talbert ◽  
J.D. Mattice

Two-year-old highbush blueberry bushes (Vaccinium corymbosum L. `Collins') were treated in Mar. 1985 with diuron or simazine at 2.2 or 4.5 kg a.i./ha. No residues were detected by reverse-phase high-performance liquid chromatography-ultraviolet absorbance detection (HPLC-UV) from treated berries that were harvested in June. Methiocarb was applied in May 1986 at 0.84 and 3 kg·ha-1 over the top of 3-year-old `Collins' when the berries began to ripen. Reverse-phase HPLC-UV of berries treated with methiocarb at 3 kg·ha-1 had combined residues of methiocarb and its sulfone and sulfoxide metabolites of 13.1 ppm from unrinsed and 7 ppm from rinsed berries harvested on the day of treatment; 4.9 ppm from unrinsed and 4 ppm from rinsed berries harvested 4 days after treatment; and 2.4 ppm from unrinsed and 2.5 ppm from rinsed berries harvested 8 days after treatment. Unrinsed berries treated with methiocarb at 0.84 kg·ha-1 had 5.7 ppm residue on the day of treatment and 1 ppm 8 days later. Residues from berries treated with methiocarb at 0.84 or 3 kg·ha-1 were below the legal tolerance level of 5 ppm after the required 7-day waiting period. Chemical names used: n'-(3,4-dichlorophenyl)-N,N -dimethylurea (diuron); 6-chloro- N,N' -diethyl-1,3,5-triazine-2,4-diamine (simazine); 3,5-dimethyl-4-(methylthio)phenol methylcarbamate (methiocarb).


HortScience ◽  
1992 ◽  
Vol 27 (10) ◽  
pp. 1111-1112 ◽  
Author(s):  
J.F. Hancock ◽  
K. Haghighi ◽  
S.L. Krebs ◽  
J.A. Flore ◽  
A.D. Draper

Seven highbush blueberry (Vaccinium corymbosum L.) cultivars were evaluated for their photosynthetic heat stability. Ail showed significant reductions in CO2 assimilation rates (A) as leaf temperatures were raised from 20 to 30C, although `Blue-crop', `Jersey', `Elliot', and `Rubel' (22% to - 27%) were significantly less affected than Spartan', `Bluejay', and `Patriot' (-41% to -51%). To determine whether temperature adaptations of highbush types can be broadened through hybridization with native, heat-tolerant species, `Bluecrop' was crossed with the V. darrowi Camp. selection Florida 4B, and F2, BC1, and BC2, populations were generated. This approach showed promise as genotypes were identified in all the derivative populations that were more heat tolerant than `Bluecrop' and had a high A.


2021 ◽  
Vol 48 (No. 4) ◽  
pp. 183-189
Author(s):  
Aruma B.H.K. De Silva ◽  
H.P. Vasantha Rupasinghe

The haskap has been successfully cultivated in many geographical zones in Canada. We investigated whether the latitude has any impact on the anthocyanin accumulation and total antioxidant capacity in the haskap berry. The measured quality attributes were compared with that of the blueberry. Haskap ‘Aurora’ berries were collected from Yukon, British Columbia, Saskatchewan, and Nova Scotia in Canada, and blueberries were collected from Nova Scotia. Cyanidin-3-O-glucoside (C3G), the most abundant anthocyanin of haskap berry, the total anthocyanins, the total polyphenols, the soluble solids (°Brix), the pH value, the total antioxidant capacity, and the moisture content were determined. The results revealed that the total anthocyanin, total phenolic content, and antioxidant capacity of the haskap range between 88 to 273 mg C3GE/100 g fresh weight (FW), 256 to 442 mg GAE/100 g of FW, and 27 to 52 µmol TE/g FW, respectively. The liquid chromatography-mass spectrometry (UPLC/MS) analysis revealed that the C3G contained in the haskap berry is highly dependent on the harvest stage. Among the samples, the haskap berry harvested on July 19, 2019, from the Yukon had the highest C3G content. Interestingly, the total anthocyanin content of the haskap berry is comparable to that of the lowbush blueberry, but higher than the highbush blueberry. A systematic multi-year experiment employing many cultivars and growing locations is recommended to better understand the latitude effect.


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