scholarly journals In Situ Quantification of Polyhydroxybutyrate in Photobioreactor Cultivations of Synechocystis sp. Using an Ultrasound-Enhanced ATR-FTIR Spectroscopy Probe

2021 ◽  
Vol 8 (9) ◽  
pp. 129
Author(s):  
Philipp Doppler ◽  
Christoph Gasser ◽  
Ricarda Kriechbaum ◽  
Ardita Ferizi ◽  
Oliver Spadiut
Keyword(s):  
Process Analytical Technology ◽  
Attenuated Total Reflection ◽  
Monitoring Tool ◽  
Biotechnological Production ◽  
Promising Alternative ◽  
Production Processes ◽  
Spectroscopic Probes ◽  
Phb Production ◽  
Analytical Technology

Polyhydroxybutyrate (PHB) is a very promising alternative to most petroleum-based plastics with the huge advantage of biodegradability. Biotechnological production processes utilizing cyanobacteria as sustainable source of PHB require fast in situ process analytical technology (PAT) tools for sophisticated process monitoring. Spectroscopic probes supported by ultrasound particle traps provide a powerful technology for in-line, nondestructive, and real-time process analytics in photobioreactors. This work shows the great potential of using ultrasound particle manipulation to improve spectroscopic attenuated total reflection Fourier-transformed mid-infrared (ATR-FTIR) spectra as a monitoring tool for PHB production processes in photobioreactors.

scholarly journals Improved Production and In Situ Recovery of Sesquiterpene (+)-Zizaene from Metabolically-Engineered E. coli

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3356 ◽  
Author(s):  
Francisco Aguilar ◽  
Thomas Scheper ◽  
Sascha Beutel
Keyword(s):  
Liquid Phase ◽  
Product Recovery ◽  
Expanded Bed Adsorption ◽  
Biotechnological Production ◽  
Promising Alternative ◽  
Phase Partitioning ◽  
In Situ Product Recovery ◽  
Solid Liquid ◽  
In Situ Recovery

The sesquiterpene (+)-zizaene is the direct precursor of khusimol, the main fragrant compound of the vetiver essential oil from Chrysopogon zizanioides and used in nearly 20% of men’s fine perfumery. The biotechnological production of such fragrant sesquiterpenes is a promising alternative towards sustainability; nevertheless, product recovery from fermentation is one of the main constraints. In an effort to improve the (+)-zizaene recovery from a metabolically-engineered Escherichia coli, we developed an integrated bioprocess by coupling fermentation and (+)-zizaene recovery using adsorber extractants. Initially, (+)-zizaene volatilization was confirmed from cultivations with no extractants but application of liquid–liquid phase partitioning cultivation (LLPPC) improved (+)-zizaene recovery nearly 4-fold. Furthermore, solid–liquid phase partitioning cultivation (SLPPC) was evaluated by screening polymeric adsorbers, where Diaion HP20 reached the highest recovery. Bioprocess was scaled up to 2 L bioreactors and in situ recovery configurations integrated to fermentation were evaluated. External recovery configuration was performed with an expanded bed adsorption column and improved (+)-zizaene titers 2.5-fold higher than LLPPC. Moreover, internal recovery configuration (IRC) further enhanced the (+)-zizaene titers 2.2-fold, whereas adsorption velocity was determined as critical parameter for recovery efficiency. Consequently, IRC improved the (+)-zizaene titer 8.4-fold and productivity 3-fold from our last report, achieving a (+)-zizaene titer of 211.13 mg L−1 and productivity of 3.2 mg L−1 h−1. This study provides further knowledge for integration of terpene bioprocesses by in situ product recovery, which could be applied for many terpene studies towards the industrialization of fragrant molecules.

In situ real time monitoring of emulsification and homogenization processes for vaccine adjuvants

The Analyst ◽  
2022 ◽  
Author(s):  
Nicole M. Ralbovsky ◽  
Randal J. Soukup ◽  
Justin P. Lomont ◽  
Mackenzie L. Lauro ◽  
Amanda Gulasarian ◽  
...  
Keyword(s):  
Real Time ◽  
Process Analytical Technology ◽  
Vaccine Adjuvant ◽  
Formation Processes ◽  
Vaccine Adjuvants ◽  
Stable Emulsion ◽  
Real Time Monitoring ◽  
Analytical Technology

Process analytical technology was used to monitor formation of a stable emulsion product, with results providing improved understanding of emulsion-based vaccine adjuvant formation processes.

Evaluation of Heat Flux Measurement as a New Process Analytical Technology Monitoring Tool in Freeze Drying

2017 ◽  
Vol 106 (5) ◽  
pp. 1249-1257 ◽  
Author(s):  
Ilona Vollrath ◽  
Victoria Pauli ◽  
Wolfgang Friess ◽  
Angelika Freitag ◽  
Andrea Hawe ◽  
...  
Keyword(s):  
Heat Flux ◽  
Freeze Drying ◽  
Process Analytical Technology ◽  
Flux Measurement ◽  
Monitoring Tool ◽  
Heat Flux Measurement ◽  
New Process ◽  
Analytical Technology

scholarly journals Tracing the Acetalization of Cyclohexanone in CO2-Expanded Alcohols by Attenuated Total Reflection Infrared Spectroscopy

2009 ◽  
Vol 63 (9) ◽  
pp. 1008-1014 ◽  
Author(s):  
Tsunetake Seki ◽  
Jean-Michel Andanson ◽  
Fabian Jutz ◽  
Alfons Baiker
Keyword(s):  
Ir Spectroscopy ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Znse Crystal ◽  
Promising Alternative ◽  
Practical Tool ◽  
Acidic Conditions ◽  
Kinetics Of

The CO2-catalyzed acetalization is regarded as a promising alternative to the conventional acid-catalyzed method from a viewpoint of green chemistry (C. A. Eckert et al., Ind. Eng. Chem. Res. 43, 2605 (2004)). We have applied in situ attenuated total reflection infrared (ATR-IR) spectroscopy for elucidating and monitoring the acetalization of cyclohexanone in CO2-expanded ethylene glycol and methanol at 50 °C and 3 MPa. The ATR-IR spectra of the reaction mixtures periodically recorded with a ZnSe crystal demonstrate that ATR-IR spectroscopy is a practical tool for tracing the kinetics of acetalizations in situ. In addition, the rate of CO2 dissolution as well as CO2 solubility into the cyclohexanone–alcohol mixtures could be evaluated from the CO2-v3-antisymmetric stretching band. The ZnSe ATR crystal, however, was corroded during longer use under the acidic conditions realized by the dissolution of CO2 in the alcohols. In contrast, the corrosion did not occur when a Ge crystal was used instead of a ZnSe crystal, and therefore the application of a Ge ATR crystal is recommended for continuous long-term experiments with these media.

scholarly journals Process analytical technology (PAT) as a versatile tool for real-time monitoring and kinetic evaluation of photocatalytic reactions

2020 ◽  
Vol 5 (10) ◽  
pp. 1992-2002
Author(s):  
Martin Rößler ◽  
Philipp U. Huth ◽  
Marcel A. Liauw
Keyword(s):  
Raman Spectroscopy ◽  
Real Time ◽  
Multivariate Data Analysis ◽  
Process Analytical Technology ◽  
Real Time Monitoring ◽  
Kinetic Evaluation ◽  
Versatile Tool ◽  
Photocatalytic Reactions ◽  
Analytical Technology

Combining in situ Raman spectroscopy with multivariate data analysis enables the real-time monitoring and kinetic evaluation of photocatalytic reactions. The applicability is demonstrated on the photooxidation of 4-methoxythiophenol.

scholarly journals Engineered Phage Endolysin Eliminates Gardnerella Biofilm without Damaging Beneficial Bacteria in Bacterial Vaginosis Ex Vivo

Pathogens ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 54
Author(s):  
Christine Landlinger ◽  
Lenka Tisakova ◽  
Vera Oberbauer ◽  
Timo Schwebs ◽  
Abbas Muhammad ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Bactericidal Activity ◽  
High Selectivity ◽  
Ex Vivo ◽  
Vaginal Microbiome ◽  
Wild Type ◽  
Wild Type Enzyme ◽  
Promising Alternative ◽  
First Time

Bacterial vaginosis is characterized by an imbalance of the vaginal microbiome and a characteristic biofilm formed on the vaginal epithelium, which is initiated and dominated by Gardnerella bacteria, and is frequently refractory to antibiotic treatment. We investigated endolysins of the type 1,4-beta-N-acetylmuramidase encoded on Gardnerella prophages as an alternative treatment. When recombinantly expressed, these proteins demonstrated strong bactericidal activity against four different Gardnerella species. By domain shuffling, we generated several engineered endolysins with 10-fold higher bactericidal activity than any wild-type enzyme. When tested against a panel of 20 Gardnerella strains, the most active endolysin, called PM-477, showed minimum inhibitory concentrations of 0.13–8 µg/mL. PM-477 had no effect on beneficial lactobacilli or other species of vaginal bacteria. Furthermore, the efficacy of PM-477 was tested by fluorescence in situ hybridization on vaginal samples of fifteen patients with either first time or recurring bacterial vaginosis. In thirteen cases, PM-477 killed the Gardnerella bacteria and physically dissolved the biofilms without affecting the remaining vaginal microbiome. The high selectivity and effectiveness in eliminating Gardnerella, both in cultures of isolated strains as well as in clinically derived samples of natural polymicrobial biofilms, makes PM-477 a promising alternative to antibiotics for the treatment of bacterial vaginosis, especially in patients with frequent recurrence.

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