AbstractDetermining which proteins interact together is crucial to a systems-level understanding of the cell. Recently, algorithms based on Direct Coupling Analysis (DCA) pairwise maximum-entropy models have allowed to identify interaction partners among the paralogs of ubiquitous prokaryotic proteins families, starting from sequence data alone. Since DCA allows to infer the three-dimensional structure of protein complexes, its success in predicting protein-protein interactions could be mainly based on contacting residues coevolving to remain physicochemically complementary. However, interacting proteins often possess similar evolutionary histories, which also gives rise to correlations among their sequences. What is the role of purely phylogenetic correlations in the performance of DCA-based methods to infer interaction partners? To address this question, we employ controlled synthetic data that only involves phylogeny and no interactions or contacts. We find that DCA accurately identifies the pairs of synthetic sequences that only share evolutionary history. It performs as well as methods explicitly based on sequence similarity, and even slightly better with large and accurate training sets. We further demonstrate the ability of these various methods to correctly predict pairings among actual paralogous proteins with genome proximity but no known direct physical interaction, which illustrates the importance of phylogenetic correlations in real data. However, for actually interacting and strongly coevolving proteins, DCA and mutual information outperform sequence similarity.Author summaryMany biologically important protein-protein interactions are conserved over evolutionary time scales. This leads to two different signals that can be used to computationally predict interactions between protein families and to identify specific interaction partners. First, the shared evolutionary history leads to highly similar phylogenetic relationships between interacting proteins of the two families. Second, the need to keep the interaction surfaces of partner proteins biophysically compatible causes a correlated amino-acid usage of interface residues. Employing simulated data, we show that the shared history alone can be used to detect partner proteins. Similar accuracies are achieved by algorithms comparing phylogenetic relationships and by coevolutionary methods based on Direct Coupling Analysis, which are a priori designed to detect the second type of signal. Using real sequence data, we show that in cases with shared evolutionary but without known physical interactions, both methods work with similar accuracy, while for physically interacting systems, methods based on correlated amino-acid usage outperform purely phylogenetic ones.
On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins
