scholarly journals Surface Bioactivation of Polyether Ether Ketone (PEEK) by Sulfuric Acid and Piranha Solution: Influence of the Modification Route in Capacity for Inducing Cell Growth

Biomolecules ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1260
Author(s):  
Flavia Suzany Ferreira dos Santos ◽  
Mariana Vieira ◽  
Henrique Nunes da Silva ◽  
Helena Tomás ◽  
Marcus Vinícius Lia Fook
Keyword(s):  
Contact Angle ◽  
Cell Adhesion ◽  
Sulfuric Acid ◽  
Cell Growth ◽  
Sulfuric Acid Treatment ◽  
Piranha Solution ◽  
Polyether Ether Ketone ◽  
Cell Growth And Proliferation ◽  
Contact Angle Analysis ◽  
Cell Adhesion And Proliferation

The aim of this study was to promote bioactivity of the PEEK surface using sulfuric acid and piranha solution. PEEK was functionalized by a sulfuric acid treatment for 90 s and by piranha solution for 60 and 90 s. Chemical modification of the PEEK surface was evaluated by infrared spectroscopy, contact angle analysis, cytotoxicity, cell adhesion and proliferation. The spectroscopy characteristic band associated with sulfonation was observed in all treated samples. PEEK with piranha solution 60 s showed an increase in the intensity of the bands, which was even more significant for the longer treatment (90 s). The introduction of the sulfonic acid functional group reduced the contact angle. In cytotoxicity assays, for all treatments, the number of viable cells was higher when compared to those of untreated PEEK. PEEK treated with sulfuric acid and piranha solution for 60 s were the treatments that showed the highest percentage of cell viability with no statistically significant differences between them. The modified surfaces had a greater capacity for inducing cell growth, indicative of effective cell adhesion and proliferation. The proposed chemical modifications are promising for the functionalization of PEEK-based implants, as they were effective in promoting bioactivation of the PEEK surface and in stimulating cell growth and proliferation.

scholarly journals Adhesion and Proliferation of Osteoblast-Like Cells on Porous Polyetherimide Scaffolds

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Yanbo Zhang ◽  
Ruiyan Li ◽  
Wenzheng Wu ◽  
Yun’an Qing ◽  
Xiongfeng Tang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Contact Angle ◽  
Cell Adhesion ◽  
Phase Composition ◽  
Cell Morphology ◽  
Solvent Casting ◽  
Water Contact ◽  
Particulate Leaching ◽  
Cell Adhesion And Proliferation ◽  
Better Than

The purpose of this work was to investigate the porous polyetherimide scaffold (P-PEIs) as an alternative biopolymer for bone tissue engineering. The P-PEIs was fabricated via solvent casting and particulate leaching technique. The morphology, phase composition, roughness, hydrophilicity, and biocompatibility of P-PEIs were evaluated and compared with polyetherimide (PEI) and Ti6Al4V disks. P-PEIs showed a biomimetic porous structure with a modulus of 78.95 ± 2.30 MPa. The water contact angle of P-PEIs was 75.4 ± 3.39°, which suggested that P-PEIs had a wettability surface. Moreover, P-PEIs provides a feasible environment for cell adhesion and proliferation. The relative cell adhesion capability and the cell morphology on P-PEIs were better than PEI and Ti6Al4V samples. Furthermore, the MC3T3-E1 cells on P-PEIs showed faster proliferation rate than other groups. It was revealed that the P-PEIs could be a potential material for the application of bone regeneration.

scholarly journals Contact Angle and Cell Adhesion of Micro/Nano-Structured Poly (Lactic-Co-Glycolic Acid) Membranes for Dental Regenerative Therapy

2021 ◽  
Vol 9 (11) ◽  
pp. 124
Author(s):  
Naoyuki Kaga ◽  
Hiroki Fujimoto ◽  
Sho Morita ◽  
Yuichiro Yamaguchi ◽  
Takashi Matsuura
Keyword(s):  
Electron Microscopy ◽  
Contact Angle ◽  
Cell Adhesion ◽  
Tissue Regeneration ◽  
Glycolic Acid ◽  
Guided Bone Regeneration ◽  
Regenerative Therapy ◽  
Regenerative Dentistry ◽  
Contact Angle Analysis ◽  
Scanning Electron

Biodegradable membranes are used in regenerative dentistry for guided tissue regeneration (GTR) and guided bone regeneration (GBR). In this study, patterned poly (lactic-co-glycolic acid) (PLGA) membranes with groove, pillar, and hole structures were successfully fabricated by thermal nanoimprinting. Their surfaces were evaluated for topography by scanning electron microscopy and laser microscopy, for hydrophobicity/hydrophilicity by contact angle analysis, and for MC3T3-E1 cell adhesion. The sizes of the patterns on the surfaces of the membranes were 0.5, 1.0, and 2.0 μm, respectively, with the height/depth being 1.0 μm. The pillared and holed PLGA membranes were significantly more hydrophobic than the non-patterned PLGA membranes (p < 0.05). However, the 0.5 μm- and 1.0 μm-grooved PLGA membranes were significantly more hydrophilic than the non-patterned PLGA membranes (p < 0.05). The 0.5 μm-grooved, pillared, and holed membranes exhibited significantly superior adhesion to the MC3T3-E1 cells than the non-patterned PLGA (p < 0.05). These results suggest that patterned PLGA membranes can be clinically used for GTR and GBR in the dental regeneration field.

Preparation and Biocompatibility of Polydopamine Modified Porous Polyetheretherketone Implants

2019 ◽  
Vol 9 (12) ◽  
pp. 1676-1684
Author(s):  
Shuang Qin ◽  
Zhengkuan Lu ◽  
Liang Chen ◽  
Yue Wang ◽  
Jianfei Bai ◽  
...  
Keyword(s):  
Contact Angle ◽  
Sulfuric Acid ◽  
Control Group ◽  
Potential Candidate ◽  
Implant Surface ◽  
X Ray ◽  
Modification Method ◽  
Surface Contact Angle ◽  
Biocompatibility Study ◽  
Cell Adhesion And Proliferation

Polyetheretherketone (PEEK), as a new polymer biomaterial, became a potential candidate for orthopedic and dental implant, however its application was limited by bioinert surface. The surface modification method had achieved remarkable results in improving the bioactivity of the implant surface. In this work, PEEK was firstly etched by sulfuric acid of different time and then the optimal group was modified with polydopamine coating. All the specimens including the control group were characterized by scanning electron microscopy (SEM), contact angle goniometer and X-ray photoelectron spectrometer (XPS). As a result, the surface of PEEK was corroded by sulfuric acid to obtain a relatively stable porous structure at 6 minutes and polydopamine coating significantly reduced the surface contact angle. In subsequent biocompatibility study, the non-toxicity and the promotion of cell adhesion and proliferation of the modified PEEK-based substrates were verified by MC3T3 cells, laying a foundation for the wider application of PEEK in medical implantation.

scholarly journals Antifouling and Flux Enhancement of Reverse Osmosis Membrane by Grafting Poly (3-Sulfopropyl Methacrylate) Brushes

Membranes ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 213
Author(s):  
Reema Mushtaq ◽  
Muhammad Asad Abbas ◽  
Shehla Mushtaq ◽  
Nasir M. Ahmad ◽  
Niaz Ali Khan ◽  
...  
Keyword(s):  
Contact Angle ◽  
Reverse Osmosis ◽  
Polymer Brushes ◽  
Surface Composition ◽  
Reverse Osmosis Membrane ◽  
Optical Profilometry ◽  
Thin Film Composite ◽  
Membrane Sample ◽  
Contact Angle Analysis ◽  
And Staphylococcus Aureus

A commercial thin film composite (TFC) polyamide (PA) reverse osmosis membrane was grafted with 3-sulfopropyl methacrylate potassium (SPMK) to produce PA-g-SPMK by atom transfer radical polymerization (ATRP). The grafting of PA was done at varied concentrations of SPMK, and its effect on the surface composition and morphology was studied by Fourier-Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), optical profilometry, and contact angle analysis. The grafting of hydrophilic ionically charged PSPMK polymer brushes having acrylate and sulfonate groups resulted in enhanced hydrophilicity rendering a reduction of contact angle from 58° of pristine membrane sample labeled as MH0 to 10° for a modified membrane sample labeled as MH3. Due to the increased hydrophilicity, the flux rate rises from 57.1 L m−2 h−1 to 71.2 L m−2 h−1, and 99% resistance against microbial adhesion (Escherichia coli and Staphylococcus aureus) was obtained for MH3 after modification

scholarly journals Enhanced Myc Expression in Silkworm Silk Gland Promotes DNA Replication and Silk Production

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 361
Author(s):  
Wenliang Qian ◽  
Yan Yang ◽  
Zheng Li ◽  
Yuting Wu ◽  
Xuechuan He ◽  
...  
Keyword(s):  
Dna Replication ◽  
Cell Growth ◽  
Silk Gland ◽  
Biological Functions ◽  
Gland Cells ◽  
Silk Production ◽  
Myc Gene ◽  
Cell Growth And Proliferation ◽  
Silkworm Silk ◽  
Posterior Silk Gland

Silkworm is an economically important insect that synthetizes silk proteins for silk production in silk gland, and silk gland cells undergo endoreplication during larval period. Transcription factor Myc is essential for cell growth and proliferation. Although silkworm Myc gene has been identified previously, its biological functions in silkworm silk gland are still largely unknown. In this study, we examined whether enhanced Myc expression in silk gland could facilitate cell growth and silk production. Based on a transgenic approach, Myc was driven by the promoter of the fibroin heavy chain (FibH) gene to be successfully overexpressed in posterior silk gland. Enhanced Myc expression in the PSG elevated FibH expression by about 20% compared to the control, and also increased the weight and shell rate of the cocoon shell. Further investigation confirmed that Myc overexpression increased nucleus size and DNA content of the PSG cells by promoting the transcription of the genes involved in DNA replication. Therefore, we conclude that enhanced Myc expression promotes DNA replication and silk protein expression in endoreplicating silk gland cells, which subsequently raises silk yield.

Silk-based hybrid microfibrous mats as guided bone regeneration membranes

2021 ◽  
Author(s):  
Mi Wu ◽  
Zhengyi Han ◽  
Wen Liu ◽  
Jinrong Yao ◽  
Bingjiao Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Adhesion ◽  
Bone Regeneration ◽  
Silk Fibroin ◽  
Guided Bone Regeneration ◽  
Regenerated Silk Fibroin ◽  
Marrow Mesenchymal Stem Cells ◽  
Cell Adhesion And Proliferation

LAPONITE® (LAP) nanoplatelets were incorporated within a regenerated silk fibroin (RSF) microfibrous mat via electrospinning, which exhibited better cell adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) than the pristine RSF ones.

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