scholarly journals Allelic Variation Analysis at the Vernalization Response and Photoperiod Genes in Russian Wheat Varieties Identified Two Novel Alleles of Vrn-B3

Biomolecules ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1897
Author(s):  
Alina Berezhnaya ◽  
Antonina Kiseleva ◽  
Irina Leonova ◽  
Elena Salina

Heading time is an important agronomic trait affecting the adaptability and productivity of common wheat. In this study, 95 common wheat varieties from Russia and the late-maturing breeding line ‘Velut’ were tested for allelic diversity of genes having the strongest effect on heading. In this research, allelic variation at the Ppd-D1, Vrn-A1, Vrn-B1, Vrn-D1, and Vrn-B3 loci was tested. The Vrn-B1 and Vrn-B3 loci provided the largest contribution to genetic diversity. We found two novel allelic variants of the Vrn-B3 gene in the studied varieties. Ten varieties carried a 160 bp insertion in the promoter region, and the breeding line ‘Velut’ carried a 1617 bp insertion. These alleles were designated Vrn-B3e and Vrn-B3d, respectively. The analysis of the sequences showed the recent insertion of a retrotransposon homologous to the LTR retrotransposon (RLX_Hvul_Dacia_ RND-1) in the Vrn-B3d allele. Plants with the Vrn-B3e and the ‘Velut’ line with the Vrn-B3d allele headed later than the plants with the wild-type allele; among these plants, ‘Velut’ is the latest maturing wheat variety. Analysis of the gene expression of two groups of lines differing by the Vrn-B3 alleles (Vrn-B3d or vrn-B3) from the F2 population with ‘Velut’ as a parental line did not reveal a significant difference in the expression level between the groups. Additional research is required to study the reasons for the late maturation of the ‘Velut’ line. However, the studied wheat varieties could be used as a potential source of natural variation in genes controlling heading times.

2019 ◽  
Vol 4 (1) ◽  
pp. 375-380
Author(s):  
Imen Klay ◽  
Leila Riahi ◽  
Hajer Slim Amara ◽  
Abderrazak Daaloul

AbstractThis study was conducted to evaluate the variability of salt tolerance potentials among nine wheat genotypes representing wild and cultivated species namely Triticum turgidum subsp. durum, Triticum aestivum and Aegilops geniculata. Ionomic and photosynthetic traits were used for the screening of the studied samples when faced with four salinity levels of NaCl (0, 50, 100 and 150 mM) under green house conditions at the seedling stage. The investigated genotypes exhibited different levels of salt stress tolerance. Ionomic and photosynthetic traits underline the distinctiveness of the common wheat varieties which highlighted particular performances under salt stress conditions and showed higher tolerance potentials among the studied genotypes. Interestingly, the Vaga variety showed more ability to maintain higher K+/Na+ ratios and Pq coefficients compared with the control conditions and stable Fv/F0 and Fv/Fm ratios. Stable behaviour was exhibited by wild Aegilops accessions while durum wheat varieties have been shown to be more sensitive to salt stress. Further investigations were required for the common wheat variety Vaga, which could be useful for successful breeding and biotechnological improvement strategies concerning wheat species.


2021 ◽  
Vol 845 (1) ◽  
pp. 012065
Author(s):  
V S Rubets ◽  
V V Pylnev ◽  
I N Voronchihina ◽  
Yu N Kotenko ◽  
D D Taranova

Abstract Creating of populations with wide genotypic diversity is the basis of successful selection of promising lines. This can be achieved by involving breeding material from various ecological and geographical origins, as well as interspecific hybrids, in crossing. 14 spring common wheat (Triticum aestivum L.) varieties of CIMMYT breeding (Mexico) were researched to identify the genetic sources of economically important features. A number of these varieties proved to be the sources of adaptability to biotic and abiotic stresses and high grain quality. The yield properties of the Mexican varieties in the central Russia were at or below the standard – the ‘Zlata ’ wheat variety.


2020 ◽  
Vol 2 (2) ◽  
pp. p80
Author(s):  
Berhanu Meles ◽  
Chekole Nigus ◽  
Atsede Teklu ◽  
Yonas G Mariam

Participatory variety selection trials were conducted in 2018 G.C in Laelay-maichew, Tahtay-maichew and Ahferom districts of central zone of Tigrai to evaluate the performance of improved bread wheat (Triticum aestivum. L) Variety and to assess farmers’ criteria for bread wheat variety selection. Six improved bread wheat varieties (Mekelle-1, Mekelle-2, Ogolcho, Kingbird and Hedasse) including the most popular variety ‘Kakaba’ were used for the study at eighteen farmers (six from each district). The experiment was laid out using randomized complete block design at baby trial with three replications. Analysis of variance revealed a significant difference among the tested varieties for most of the agronomic traits except for kernels per spike and harvest index in all the tested locations. In the preference ranking, farmers used their own traits of interest which were very important in their wheat varieties for selection. Hence, common criteria’s identified by the farmers to select the best varieties were; grain yield, biomass yield, earliness, disease resistance, spike length and seed size. Accordingly direct matrix ranking by farmers showed that Ogolcho was top ranked both at L/maichew and Ahferom followed by Kakaba, however Mekelle-1 was first ranked followed by Kakaba at T/maichew.  Therefore farmers of L/maichew and Ahferom were recommended to use Ogolcho and Kakaba, whereas Mekelle-1 was recommended for T/maichew.


2019 ◽  
Vol 24 ◽  
pp. 284-289
Author(s):  
T. O. Sobko ◽  
G. M. Lisova ◽  
L. V. Sirant

Aim. The aim of the study was to investigate genetic variability of storage protein loci in European common winter wheat varieties licensed for growing in Ukraine in 2018 year. Methods. SDS and APAG electrophoresis were used to identify genotypes at the high-molecular-weight (HMW) glutenin loci Glu-1 and gliadin loci Gli-1. Results. Genotypes at the gliadin loci Gli-A1, Gli-B1, Gli-D1 and HMW glutenin loci Glu-A1, Glu-B1, Glu-D1 were identified in 48 winter common wheat varieties. A total of twenty gliadin alleles (including 10 at the Gli-A1, 4 at the Gli-B1, 6 at the Gli-D1 loci) and eleven HMW-GS alleles (including 3 at the Glu-A1, 5 at the Glu-B1, 3 at the Glu-D1 loci) were revealed. Differences in frequencies of alleles at storage protein loci were established. Conclusions. The high level of allelic variation was observed at the Gli-1 and Glu-1 loci in European common winter wheat varieties. Predominant alleles were revealed: Gli-A1о, Gli-A1а, Gli-A1f, Gli-B1f, Gli-D1b, Gli-D1d, Glu-А1c, Glu-А1a, Glu-B1с, Glu-B1d, Glu-D1d, Glu-D1a. Differences in frequencies of alleles at the storage protein loci between European and Ukrainian common winter wheat varieties were found. Keywords: Triticum aestivum L., winter wheat, storage protein, alleles.


2018 ◽  
Vol 23 ◽  
pp. 334-339
Author(s):  
T. O. Sobko ◽  
L. V. Sirant ◽  
G. M. Lisova

Aim. The aim of this study was to investigate genetic diversity of storage protein loci in spring common wheat varieties licensed for growing in Ukraine in 2017 year. Methods. SDS and APAG electrophoresis were used to identify genotypes at the gliadin loci Gli-1 and the high-molecular-weight glutenin subunit loci Glu-1. Results. Genotypes at the gliadin loci Gli A1, Gli B1, Gli D1 and glutenin loci Glu A1, Glu B1, Glu D1 were identified in 31 spring common wheat. The wheat-rye translocation AL/1RS was identified in two varieties Etyud and Struna mironivsyka. Differences in frequencies of alleles at storage protein loci were revealed. Conclusions. The high level of allelic variation was observed at the Gli-1 loci in spring common wheat varieties. Predominant alleles (one or two per locus) were revealed: Gli A1f, Gli A1а, Gli B1e, Gli D1b, Gli D1і, Gli D1f , Glu А1а, Glu Bс , Glu D1d, Glu D1a. Keywords: Triticum aestivum L., spring wheat, storage protein.


2020 ◽  
Vol 27 ◽  
pp. 270-275
Author(s):  
T. O. Sobko ◽  
G. M. Lisova ◽  
L. V. Sirant

Aim. The aim of the study was to investigate genetic variability of gliadin-coding loci Gli-A1, Gli-B1, Gli-D1 in Ukrainian winter common wheat landraces and obsolete varieties. Methods. APAG electrophoresis of gliadin was used to identify genotypes at Gli-1 loci. Results. Diversity at the Gli-1 loci of 41 winter common wheat landraces, including local varieties Krymka, Banatka, Girka, Theyka, and 13 obsolete winter common wheat varieties, were studied. A total 31 gliadin alleles (including new 7 alleles) were revealed. There are 11 alleles at the Gli-A1 loci, 14 - at the Gli-B1, 6 -at the Gli-D1. Differences in frequencies of gliadin alleles were established. Conclusions. The high level of allelic variation at the Gli-1 loci was observed in Ukrainian winter common wheat landraces. Predominant alleles were revealed: Gli-A1m (46,8 %), Gli-A1d (21 %), Gli-B1b (47,1 %), Gli-D1g (45,8 %), Gli-D1a (33,3 %), Gli-D1j (13,9 %). In genotype of modern Ukrainian winter common wheat are present only 3 of them - Gli-B1b, Gli-D1g, Gli-D1j, which positively relate to dough quality. Keywords: Triticum aestivum L., winter wheat, landraces, gliadin , alleles , polymorphism


2009 ◽  
Vol 35 (9) ◽  
pp. 1606-1612
Author(s):  
Ping-Ping ZHANG ◽  
Hong-Xiang MA ◽  
Jin-Bao YAO ◽  
Zhong-Hu HE

2021 ◽  
Vol 16 (1) ◽  
pp. 641-652
Author(s):  
Sławomir Franaszek ◽  
Bolesław Salmanowicz

Abstract The main purpose of this research was the identification and characterization of low-molecular-weight glutenin subunit (LMW-GS) composition in common wheat and the determination of the effect of these proteins on the rheological properties of dough. The use of capillary zone electrophoresis and reverse-phase high-performance liquid chromatography has made it possible to identify four alleles in the Glu-A3 and Glu-D3 loci and seven alleles in the Glu-B3 locus, encoding LMW-GSs in 70 varieties and breeding lines of wheat tested. To determine the technological quality of dough, analyses were performed at the microscale using a TA.XT Plus Texture Analyzer. Wheat varieties containing the Glu-3 loci scheme (Glu-A3b, Glu-A3f at the Glu-A3 locus; Glu-B3a, Glu-B3b, Glu-B3d, Glu-B3h at the Glu-B3 locus; Glu-D3a, Glu-D3c at the Glu-D3 locus) determined the most beneficial quality parameters.


2019 ◽  
Vol 11 (12) ◽  
pp. 3378
Author(s):  
Charity Ruramai Nhemachena ◽  
Johann F. Kirsten ◽  
Binganidzo Muchara

The strengthening of the intellectual property rights (IPRs) for plant varieties provide incentives for breeding companies to invest more resources in plant breeding. The main objective of this paper was to analyze the effects of strengthening the wheat variety intellectual protection on wheat productivity and the release of new varieties. The strength of IPR systems was measured using an intellectual property (IP) protection index, and plant breeders’ rights (PBRs) granted for wheat varieties. The empirical analyses were based on correlation and multiple regression analyses. The results showed that strengthening IPR systems in South Africa contribute to improving wheat productivity and increasing the number of wheat varieties released. Furthermore, although the robust coefficients of the other IPR variables are positive, they are statistically insignificant for all scenarios. There is a need for more incentives beyond granting PBRs and strengthening of IPR systems to be provided in the whole wheat sector to stimulate increased investments and the release of new varieties.


2018 ◽  
Vol 17 (03) ◽  
pp. 213-220 ◽  
Author(s):  
Teresa Bieńkowska ◽  
Elżbieta Suchowilska ◽  
Wolfgang Kandler ◽  
Rudolf Krska ◽  
Marian Wiwart

AbstractThe grain of modern wheat cultivars has a significantly lower mineral content, including the content of copper, iron, magnesium, manganese, phosphorous, selenium and zinc. For this reason cereal breeders, are constantly searching for new genetic sources of minerals that are essential in human nutrition. Triticum polonicum, which is grown on a small scale in Spain, southern Italy, Algeria, Ethiopia and warm regions of Asia, deserves special attention in this context. The micronutrient and macronutrient content of T. polonicum versus T. durum and T. aestivum was compared in this study. Polish wheat grain was characterized by the significantly highest content of phosphorus (4.55 g/kg), sulphur (1.82 g/kg), magnesium (1.42 g/kg), zinc (49.5 mg/kg), iron (39.1 mg/kg) and boron (0.56 mg/kg) as well as a low content of aluminium (only 1.04 mg/kg). The macronutrient profile of most T. polonicum lines differed completely from that of common wheat and durum wheat. The principal component analysis supported discrimination of seven Polish wheat lines with a particularly beneficial micronutrient profile (P2, P3, P5, P7, P9, P22 and P25). These lines were characterized by the highest content of copper, iron and zinc, as well as the lowest concentrations of strontium, aluminium and barium which are undesirable in food products. The above lines can be potentially applied as source materials for breeding new wheat varieties. The results of this study indicate that Polish wheat could be used in genetic biofortification of durum wheat and common wheat.


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