scholarly journals Important Metabolites in Maintaining Folate Cycle, Homocysteine, and Polyamine Metabolism Associated with Ranibizumab Treatment in Cultured Human Tenon’s Fibroblasts

Biomolecules ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 243
Author(s):  
Siti Munirah Md Noh ◽  
Siti Hamimah Sheikh Abdul Kadir ◽  
Sushil Vasudevan
Keyword(s):  
Cell Cycle ◽  
Wound Healing ◽  
Healing Process ◽  
Polyamine Metabolism ◽  
Control Group ◽  
Wound Healing Process ◽  
P Value ◽  
Nucleotide Synthesis ◽  
Folate Cycle ◽  
Ranibizumab Treatment

The anti-fibrotic properties of ranibizumab have been well documented. As an antagonist to vascular endothelial growth factor (VEGF), ranibizumab works by binding and neutralizing all active VEGF-A, thus limiting progressive cell growth and proliferation. Ranibizumab application in ocular diseases has shown remarkable desired effects; however, to date, its antifibrotic mechanism is not well understood. In this study, we identified metabolic changes in ranibizumab-treated human Tenon’s fibroblasts (HTFs). Cultured HTFs were treated for 48 h with 0.5 mg/mL of ranibizumab and 0.5 mg/mL control IgG antibody which serves as a negative control. Samples from each group were injected into Agilent 6520 Q-TOF liquid chromatography/mass spectrometer (LC/MS) system to establish the metabolite expression in both ranibizumab treated cells and control group. Data obtained was analyzed using Agilent Mass Hunter Qualitative Analysis software to identify the most regulated metabolite following ranibizumab treatment. At p-value < 0.01 with the cut off value of two-fold change, 31 identified metabolites were found to be significantly upregulated in ranibizumab-treated group, with six of the mostly upregulated having insignificant role in fibroblast cell cycle and wound healing regulations. Meanwhile, 121 identified metabolites that were downregulated, and seven of the mostly downregulated are significantly involved in cell cycle and proliferation. Our findings suggest that ranibizumab abrogates the tissue scarring and wound healing process by regulating the expression of metabolites associated with fibrotic activity. In particular, we found that vitamin Bs are important in maintaining normal folate cycle, nucleotide synthesis, and homocysteine and spermidine metabolism. This study provides an insight into ranibizumab’s mechanism of action in HTFs from the perspective of metabolomics.

scholarly journals Important metabolites in maintaining folic acid, homocysteine and polyamine metabolism associated with ranibizumab treatment in cultured Human Tenon’s Fibroblast

2019 ◽  
Author(s):  
Siti Munirah Md Noh ◽  
Siti Hamimah Sheikh Abdul Kadir ◽  
Sushil Kumar Vasudevan
Keyword(s):  
Cell Cycle ◽  
Wound Healing ◽  
Folic Acid ◽  
Treated Group ◽  
Negative Control ◽  
Polyamine Metabolism ◽  
Control Group ◽  
Igg Antibody ◽  
Nucleotide Synthesis ◽  
Ranibizumab Treatment

Anti-fibrotic properties of ranibizumab have been well documented. As an antagonist to Vascular Endothelial Growth Factor (VEGF), ranibizumab works by binding and neutralizes all active VEGF, thus limits the progressive cell growth and proliferation. Its application in ocular diseases has shown remarkable desired effects, however to date its anti-fibrotic mechanism is not well understood. In this study, we identified metabolic changes in ranibizumab-treated Human Tenos’s fibroblast (HTF). Cultured HTFs were treated for48 hours with 0.5 mg/ml of ranibizumab and 0.5 mg/ml control IgG antibody which serves as a negative control. Samples from each group were injected into Agilent 6520 Q-TOF Liquid Chromatography/ Mass Spectrometer (LC/MS) System to establish the metabolite expression in both ranibizumab treated cells and control group. Data obtained was analysed using Agilent Mass Hunter Qualitative Analysis software to identify the most regulated metabolite following ranibizumab treatment. At statistical analysis of p-value < 0.01 with the cut off value of two-fold change, 31 identified metabolites were found to be significantly up-regulated in ranibizumab-treated group, with 6 of the mostly up-regulated have insignificant role in fibroblast’s cell cycle and wound healing regulations Meanwhile, 121 identified metabolites were down-regulated with seven of the mostly down-regulated are significantly involved in cell cycle and proliferation. Our findings demonstrated that ranibizumab abrogates the tissue scarring process and wound healing formation by regulating the expression of metabolites associated with fibrotic activity. In particular, we found that vitamin Bs are important in maintaining normal folic acid cycle, nucleotide synthesis, homocysteine and spermidine metabolism. This study provides an insight of ranibizumab mechanism of actions on HTFs from the perspective of metabolomics.

scholarly journals Important metabolites in maintaining folic acid, homocysteine and polyamine metabolism associated with ranibizumab treatment in cultured Human Tenon’s Fibroblast

2019 ◽  
Author(s):  
Siti Munirah Md Noh ◽  
Siti Hamimah Sheikh Abdul Kadir ◽  
Sushil Kumar Vasudevan
Keyword(s):  
Cell Cycle ◽  
Wound Healing ◽  
Folic Acid ◽  
Treated Group ◽  
Negative Control ◽  
Polyamine Metabolism ◽  
Control Group ◽  
Igg Antibody ◽  
Nucleotide Synthesis ◽  
Ranibizumab Treatment

Anti-fibrotic properties of ranibizumab have been well documented. As an antagonist to Vascular Endothelial Growth Factor (VEGF), ranibizumab works by binding and neutralizes all active VEGF, thus limits the progressive cell growth and proliferation. Its application in ocular diseases has shown remarkable desired effects, however to date its anti-fibrotic mechanism is not well understood. In this study, we identified metabolic changes in ranibizumab-treated Human Tenos’s fibroblast (HTF). Cultured HTFs were treated for48 hours with 0.5 mg/ml of ranibizumab and 0.5 mg/ml control IgG antibody which serves as a negative control. Samples from each group were injected into Agilent 6520 Q-TOF Liquid Chromatography/ Mass Spectrometer (LC/MS) System to establish the metabolite expression in both ranibizumab treated cells and control group. Data obtained was analysed using Agilent Mass Hunter Qualitative Analysis software to identify the most regulated metabolite following ranibizumab treatment. At statistical analysis of p-value < 0.01 with the cut off value of two-fold change, 31 identified metabolites were found to be significantly up-regulated in ranibizumab-treated group, with 6 of the mostly up-regulated have insignificant role in fibroblast’s cell cycle and wound healing regulations Meanwhile, 121 identified metabolites were down-regulated with seven of the mostly down-regulated are significantly involved in cell cycle and proliferation. Our findings demonstrated that ranibizumab abrogates the tissue scarring process and wound healing formation by regulating the expression of metabolites associated with fibrotic activity. In particular, we found that vitamin Bs are important in maintaining normal folic acid cycle, nucleotide synthesis, homocysteine and spermidine metabolism. This study provides an insight of ranibizumab mechanism of actions on HTFs from the perspective of metabolomics.

scholarly journals EFFECT OF WOUND CARE ON WOUND HEALING PROCESS OF POST OPERATION IN PATIENTS APENDEKTOMY IN RS DUSTIRA CIMAHI

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Marlin Sutrisna ◽  
Vega M Tusyanawati
Keyword(s):  
Wound Healing ◽  
Wound Care ◽  
Healing Process ◽  
Intervention Group ◽  
Lower Abdominal Pain ◽  
Control Group ◽  
Wound Healing Process ◽  
P Value ◽  
Ablative Surgery ◽  
Significant Difference

ABSTRACTVega M. Tusyanawati1, Marlin Sutrisna2, Tonika Tohri3  Sekolah Tinggi Ilmu Kesehatan Rajawali Bandung IndonesiaEmail : [email protected]: Appendicitis is one of the emergency  cases in the abdominal area with a major complaint of persistent lower abdominal pain that develops and increased pain. An appendectomy is a surgical intervention that has the purpose of ablative surgery or removal of body parts that contain problems or have disease. One of the postoperative treatments of apendectomy is with wound care. The current wound care treatment is modern and conventional wound care. Objective: This study was to investigate the effect of treatment of postoperative wound healing apendectomy. Methodology: The design used in this research is Quasi Experiment, with posttest-only design approach. The number of samples taken is 18 respondent. Taking sampling with accidental sampling technique. Result: The result shows that p-value 0.001, with mean value in intervention group was 5.50 and in control group 13.50. Conclusion: There is a significant difference in the wound healing process using conventional and modern wound care.Keywords                    : type of wound care, post operative apendectomy 

scholarly journals COMPARISON OF THE EFFECTIVENESS OF GIVING BINAHONG LEAF (Anredera cordifolia (Ten) Steenis) AND PAPAYA LEAF (Carica papaya) ON SKIN WOUND HEALING IN WHITE RAT (Rattus novergicus)

2021 ◽  
Vol 3 (2) ◽  
pp. 78-85
Author(s):  
Givenchy Winarjo ◽  
◽  
Fransiscus Arifin ◽  
Dave Oenarta ◽  
◽  
...  
Keyword(s):  
Wound Healing ◽  
Leaf Extract ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
P Value ◽  
World Population ◽  
White Rats ◽  
Significant Difference ◽  
The World

Introduction: The incidence of acute and chronic injuries increases every year. Most injuries in the world population, as many as 48%, are caused by trauma or surgery. the highest incidence of wounds in the world based on the etiology was from surgery, which was 110.30 million cases. One of the handlings of wounds on the skin can be done by giving herbal medicines, which can be easily accessed by the public, such as binahong leaves and papaya leaves. Purpose: To analyze the effectiveness of giving 40% binahong leaf extract cream and 40% papaya leaf extract cream on the wound distance in the white rat wound healing process. Method: this study is an experimental study with post-test only control group design approach. The sampling technique used in this study was a random sampling method where the number of samples was calculated using the Federer formula, with a total sample of 28 white rats. The distance of the wound was measured using a micrometer. Results: Twenty-eight wound distance on rats were measured as subjects of the study and then treated with binahong leaf extract cream and papaya leaf extract cream, then the wound distance of each treatment was measured using a micrometer. According to the results, 40% binahong leaf extract cream and 40% papaya leaf extract cream showed better effectiveness in reducing the wound distance of white rat incision than the negative control group (cream base) and positive control group (gentamicin ointment). According to the Mann Whitney test comparing the P1 group (40% binahong leaf extract cream) and P2 (40% papaya leaf extract cream), p-value >0.05, which is 0.543, which means that P1 has no significant mean difference with P2. Conclusion: There was no significant difference in effectiveness between the treatment of 40% binahong leaf extract cream and 40% papaya leaf extract cream.

scholarly journals BINAHONG LEAVES (ANREDERA CORDIFOLIA TENORE STEEN) EXTRACT AS AN ALTERNATIVE TREATMENT FOR PERINEAL WOUND HEALING OF POSTPARTUM MOTHERS

2017 ◽  
Vol 3 (6) ◽  
pp. 778-783
Author(s):  
Dita Selvia Aditia ◽  
Syarief Thaufik Hidayat ◽  
Nur Khafidhoh ◽  
Suhartono Suhartono ◽  
Ari Suwondo
Keyword(s):  
Wound Healing ◽  
Health Center ◽  
Alternative Treatment ◽  
Healing Process ◽  
Perineal Wound ◽  
Control Group ◽  
Wound Healing Process ◽  
P Value ◽  
Perineal Wound Healing ◽  
Postpartum Mothers

Background: Perineal wounds are at risk for infection, without a good treatment, perineal wound can harbor sepsis and become a chronic, long-term problem. Binahong leaves is considered as an alternative to accelerate perineal wound healing process.Objective: To determine the effect of binahong leaf extract (anredera cordifolia (tenore) steen) on perineal wound healing process in postpartum mothers.Methods: This study employed a quasy experimental study with control group posttest-only design. The study was conducted in the working area of Telogosari Wetan Health Center and Health Center of Kulon Kota Semarang in January-February 2017. There were 36 respondents selected using purposive sampling assigned in the intervention group (Binahong leaves extract) and the control group (Povidon Iodine 10%). REEDA scale was used for assessing perineal healing. Data were analyzed using Mann Whitney, Chi Square and Kruskall Wallis test.Results: Findings showed that the average day of perineal wound healing process in the binahong leaves group was 6.11 days and in the povidon iodine group was 7.66 days. There was a statistically significant difference in the duration of wound healing between the experiment and control group with p-value 0,001 (p value <0.05).Conclusion: Binahong leaves extract is effective in healing perineal wound in postpartum mothers. Therefore, binahong leaves are recommended to use as an alternative treatment for the mothers.

scholarly journals The Effect Of Erythropoietin Administration In Experimental Burns Wound Healing: An Animal Study

2016 ◽  
Vol 3 (1) ◽  
pp. 1-8
Author(s):  
Afriyanti Sandhi ◽  
Aditya Wardhana
Keyword(s):  
Wound Healing ◽  
Animal Study ◽  
Low Dose ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
P Value ◽  
High Dose ◽  
Sprague Dawley ◽  
Surface Areas

Background: The hematopoietic growth factor erythropoietin (EPO) attracts attention due to its all-tissue-protective pleiotropic properties. The purpose of this study is to investigate the effect of EPO in experimental burn wounds healing. Methods: Fifteen healthy Sprague-Dawley, strain of Rattus Novergicus weighing 300-350 grams, were prepared to achieve deep dermal burns. Animals were randomized to receive either low-dose EPO injection (600 IU/mL), high-dose EPO injection (3000 IU/mL) or nothing (control group). After 14 days of observations, quantitative and qualitative assessments of wound healing was determined. Results: The size of the wound area and re-epithelialization rate percentage was determined on Day-0, Day-5, Day-10, and Day-14. The average of raw surface areas measurement (p value: 0.012 in day-5; 0.009 in day-10 and 0.000 in day-14) and healing percentage of the lesions (p value: 0.011 in day-5; 0.016 in day-10 and 0.010 in day-14) were significantly best in the low-dose EPO grup compared to the control group and high-dose EPO grup. The histopathology evaluation revealed that the highest score for for re-epithelialization, granulation tissue and neo-angiogenesis were achieved by the low-dose EPO injection group than in both control and high-dose EPO injection groups. Conclusion: In this animal study using Sprague-Dawley rats, Recombinant Human EPO (rHuEPO) injection administration prompted the evidences of improved re-epithelialization and wound healing process of the skin caused by deep dermal burns. These findings may lead to a new therapeutic approach to improve the clinical outcomes for the management of burns wound healing.

scholarly journals BREAST MILK AS AN ALTERNATIVE FOR POSTPARTUM PERINEAL CARE

2017 ◽  
Vol 3 (3) ◽  
pp. 238-245 ◽  
Author(s):  
Yuli Admasari ◽  
Bedjo Santoso ◽  
Titi Suherni ◽  
Imam Djamaluddin Mashoedi ◽  
Mardiyono Mardiyono
Keyword(s):  
Wound Healing ◽  
Breast Milk ◽  
Health Workers ◽  
Healing Process ◽  
Intervention Group ◽  
Perineal Wound ◽  
Control Group ◽  
Wound Healing Process ◽  
P Value ◽  
Perineal Laceration

Background: Perineal laceration during childbirth is very common among mothers; however some of them may suffer from its complication if not treated properly.Objective: To prove the effectiveness of breast milk as an alternative topical ingredient in the treatment of perineal wound in postpartum mothers.Methods: This was a quasi-experimental study with non-equivalent control group posttest only design. There were 30 respondents selected in this study, with 15 assigned in the intervention and control group. Accidental sampling was used to select the samples with the criteria that the respondents had perineal laceration in level 1 and 2. Data were analzed using Mann Whitney test.Results: Effective wound healing process can be seen in the intervention group from 80% of poor category in 6-10 hours (1st period) of postpartum became 86.7% of good category in 7 days of postpartum (4th period). Different from the control group that showed the slow progress of wound healing, which was 86.7% of poor category in the 1st period to only 33.3% of good category in the 4th period. Mann Whitney test showed that there was a significant mean difference of the perineal wound healing process between the intervention group (11.23) and the control group (19.77) with p-value 0.002 (<0.05).Conclusion: Breast milk was more effective than povidone iodine in the treatment of perineal wound. It is suggested for health workers, especially midwife to apply this intervention to accelerate the healing of perineal wound in midwifery care.

scholarly journals The Effect of Honey Compress Therapy Toward Skin Wound Healing for Full Thicknes Loss on Rattus Norvegicus

2015 ◽  
Vol 2 (2) ◽  
pp. 103-107
Author(s):  
Dina Zakiyyatul Fuadah ◽  
Diana Rachmania ◽  
Novita Yudik
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Skin Wound ◽  
Control Group ◽  
Wound Healing Process ◽  
P Value ◽  
Skin Wound Healing ◽  
Control Group Design ◽  
Post Test ◽  
Skin Loss

Wounds require a very effective treatment for example an open wound. Honey compress therapy is one method that can be used for wound healing process. The purpose of this study, to determine the effect of therapy on wound healing using honey compress full thicknes skin loss on Rattus norvegicus.Design for the research is True Experiments using post-test design with control group design. Samples were 18. Sampling using random sampling techniques. The independent variable is the honey compress therapy and the dependent variable is the process of wound healing of full thicknes skin loss. Data were analyzed using the Mann Whitney test (α = 0.05).The results of the statistical test on day 15, p value = 0.004 <α that’s meanthere are significant differences between the treatment group and the control group in wound healing of full thicknes skin loss on Rattus norvegicus.Honey compress therapy accelerates wound healing process full thicknes skin loss. It is recommended for people can take advantage of pure honey for wound healing due to the composition of honey that can help the healing process.

scholarly journals Studi Perbandingan Modern Dressing (Salep Tribee) dan Konvensional Terhadap Proses Penyembuhan Luka PADA PASIEN POST OPERASI APENDIKTOMI

2020 ◽  
Vol 4 (1) ◽  
pp. 9
Author(s):  
Vega M. Tusyanawati ◽  
Marlin Sutrisna ◽  
Tonika Tohri
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Intervention Group ◽  
Sampling Technique ◽  
Bivariate Analysis ◽  
Control Group ◽  
Wound Healing Process ◽  
P Value ◽  
Postoperative Wound ◽  
Significant Difference

ABSTRAKPenelitian ini merupakan studi perbandingan modern dressing (salep tribee) dan konvensional terhadap proses penyembuhan luka pada pasien post operasi apendiktomi. Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh jenis perawatan luka terhadap penyembuhan luka post operasi apendiktomi. Metode: Desain yang digunakan pada penelitian ini adalah Quasy Experiment posttest-only design. Jumlah sampel sebanyak 18 orang dengan teknik accidental sampling. Pengambilan data dilakukan di Rumah Sakit Dustira Cimahi pada tanggal 19 April 2017–19 Mei 2017. Instrumen penelitian ini adalah lembar observasi penyembuhan luka. Ijin etik untuk penelitian dari STIKes Rajawali. Data dianalisis secara univariate dan bivariate. Hasil: rerata usia kelompok intervensi 29,6 tahun (SD 3,5) sedangkan pada kelompok kontrol 31,1 tahun (SD 3,4). Nilai median penyembuhan luka kelompok intervensi adalah 1,00 dan pada kelompok kontrol 3,00. Hasil analisis lebih lanjut didapatkan nilai p-value 0,001. Kesimpulan: Terdapat perbedaan yang bermakna pada proses penyembuhan luka dengan menggunakan perawatan luka konvensional dan modern.Kata kunci: Jenis perawatan luka; post operasi apendektomiA Comparative Modern Pressing (Tribee Ointment) and Conventional Pressing of Postoperative Wound Healing in Appendectomy ABSTRACTThe research is comparative study between modern dressing (salep tribee) and conventional toward healing process in post appendectomy patients. Objective: This study was to investigate the effect of treatment of postoperative wound healing appendectomy. Method: The design used in this research is Quasi Experiment, with posttest-only design approach. The number of samples were 18 selected with using accidental sampling technique. Collecting data in Dustira Hospital Cimahi between 19 April and 19 May 2017. The instrument of this research is observation form of wound healing. The ethical clearance from STIKes Rajawali Bandung. Data was analyzed with univariate and bivariate analysis. Result: The result shows the mean age of the intervention group was 29.6 years (SD 3.5) whereas in the control group 31.1 years (SD 3.4). The median value of wound healing in the intervention group was 1.00 and in the control group 3.00. Further analysis resulted in a p-value of 0.001. Conclusion: There is a significant difference in the wound healing process using conventional and modern wound care.Keywords: type of wound care; post-operative appendectomy

scholarly journals BIRD’S NEST EXTRACT CREAM: TREATMENT FOR PERINEAL WOUND IN RATTUS NORVEGICUS

2017 ◽  
Vol 3 (3) ◽  
pp. 265-271
Author(s):  
Herlina Ofiwijayanti ◽  
Syarief Thaufik Hidayat ◽  
Nur Khafidhoh
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Healing Process ◽  
Perineal Wound ◽  
Control Group ◽  
Wound Healing Process ◽  
P Value ◽  
Laboratory Unit ◽  
Perineal Wound Healing ◽  
Accelerate Wound Healing

Background: Perineal rupture occurs almost in all the first labor and not infrequently in the next labor. Complex perineal wounds are at risk for non healing and infection.Objective: This study aims to determine the effect of bird’s nest extract on perineal wound healing on rattus norvegicus.Methods: This was a randomised posttest only group design conducted in October 2016 at Animal Laboratory Unit of Diponegoro University, Semarang. There were 30 samples recruited in this study, divided into three groups: 1) The control group (with providine iodine 10% solution), 2) The  first treatment group with a bird’s nest 50% cream, and 3) The second treatment group with a bird’s nest 70% cream. The REEDA scale was used. Data were analyzed using descriptive statistics, frequency distribution, and one way anova.Results: There were statistically significant mean differences between the three groups with p-value 0.000 (< 0.05). The time of the wound healing was 5-6 days for Iodine 10% group, 4-5 days for bird’s nest 50% group, and 3 days for bird’s nest 70% group. The degree of perineal wound density in the bird’s nest 70% group (0.37) was also better than the bird’s nest 50% group (0.13) and iodine 10% group (0.02).Conclusion: Bird’s nest has a significant effect on wound healing process. Findings indicated that the bird’s nest 70% extract was very effective to accelerate wound healing than bird’s nest 50% extract and Iodine 10%.

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