The Effect Of Erythropoietin Administration In Experimental Burns Wound Healing: An Animal Study

Background: The hematopoietic growth factor erythropoietin (EPO) attracts attention due to its all-tissue-protective pleiotropic properties. The purpose of this study is to investigate the effect of EPO in experimental burn wounds healing. Methods: Fifteen healthy Sprague-Dawley, strain of Rattus Novergicus weighing 300-350 grams, were prepared to achieve deep dermal burns. Animals were randomized to receive either low-dose EPO injection (600 IU/mL), high-dose EPO injection (3000 IU/mL) or nothing (control group). After 14 days of observations, quantitative and qualitative assessments of wound healing was determined. Results: The size of the wound area and re-epithelialization rate percentage was determined on Day-0, Day-5, Day-10, and Day-14. The average of raw surface areas measurement (p value: 0.012 in day-5; 0.009 in day-10 and 0.000 in day-14) and healing percentage of the lesions (p value: 0.011 in day-5; 0.016 in day-10 and 0.010 in day-14) were significantly best in the low-dose EPO grup compared to the control group and high-dose EPO grup. The histopathology evaluation revealed that the highest score for for re-epithelialization, granulation tissue and neo-angiogenesis were achieved by the low-dose EPO injection group than in both control and high-dose EPO injection groups. Conclusion: In this animal study using Sprague-Dawley rats, Recombinant Human EPO (rHuEPO) injection administration prompted the evidences of improved re-epithelialization and wound healing process of the skin caused by deep dermal burns. These findings may lead to a new therapeutic approach to improve the clinical outcomes for the management of burns wound healing.

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Efektivitas getah batang betadine (Jatropha multifida L.) terhadap penyembuhan luka pasca pencabutan gigi pada tikus Sprague-DawleyEffectiveness of betadine (Jatropha multifida L.) stem sap on the wound healing after tooth extraction in Sprague-Dawley rats

Jurnal Kedokteran Gigi Universitas Padjadjaran ◽

10.24198/jkg.v33i2.32563 ◽

2021 ◽

Vol 33 (2) ◽

pp. 145

Author(s):

Hendry Rusdy ◽

Astri Suryani Pasaribu Saruksuk ◽

Rahmi Syaflida Dalimunte ◽

Gostry Aldica Dohude

Keyword(s):

Wound Healing ◽

Tranexamic Acid ◽

Tooth Extraction ◽

Healing Process ◽

Control Group ◽

Wound Healing Process ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Jatropha Multifida ◽

Plant Sap

Pendahuluan: Pencabutan gigi merupakan prosedur yang sering dilakukan di kedokteran gigi. Setelah pencabutan gigi akan dihasilkan suatu perlukaan. Proses penyembuhan luka dapat dipercepat pada kondisi tertentu. Salah satu bahan alami yang dapat membantu proses penyembuhan luka adalah getah tanaman betadine (Jatropha multifida L.). Penelitian bertujuan untuk menganalisis efektivitas getah tanaman betadine terhadap penyembuhan luka dan terhadap tanda-tanda infeksi pasca pencabutan gigi Metode: Desain penelitian studi eksperimental laboratorium dengan rancangan penelitian post-test only control group design menggunakan 30 ekor tikus Sprague-Dawley. Teknik pengambilan sampel yaitu convenience sampling. Sampel dibagi menjadi 2 kelompok yaitu kelompok perlakuan dan kelompok kontrol. Kelompok perlakuan diberikan getah tanaman betadine dan kelompok kontrol diberikan asam traneksamat secara oral menggunakan sonde lambung. Tunggu selama 4 jam setelah pemberian getah tanaman betadine dan asam traneksamat. Anestesi pada tikus menggunakan ketamin 50 mg/kg berat badan secara intramuskular kemudian dilakukan pencabutan pada gigi tikus. Pengamatan dilakukan dengan melihat kriteria klinis pada hari 1,3,7 dan diperhatikan sampai luka sembuh serta lihat tanda-tanda infeksi. Analisis data dilakukan dengan uji normalitasShapiro Wilik. Hasil penelitian menunjukkan bahwa data berdistribusi tidak normal. Analisis data dilanjutkan menggunakan uji statistik mann whitney. Hasil: Terdapat perbedaan signifikan penyembuhan luka soket pasca pencabutan gigi setelah diberikan getah betadine dan asam traneksamat dengan nilai p=0,037 (p<0,005). Simpulan: Pemberian getah tanaman betadine terbukti lebih efektif terhadap proses penyembuhan luka soket pasca pencabutan gigi dibandingkan dengan pemberian asam traneksamat. Kata kunci: tikus Sprague-Dawley; penyembuhan luka; pencabutan gigi; getah batang betadine ABSTRACTIntroduction: Tooth extraction is a procedure often performed in dentistry. Tooth extraction will always cause injuries. However, the wound healing process can be accelerated under certain conditions. One of the natural ingredients that can accelerate the wound healing process is betadine (Jatropha multifida L.) plant sap. The study was aimed to analyzed the effect of betadine plant sap on wound healing and signs of infection after tooth extraction. Methods: Experimental laboratory study design with post-test only control group design was conducted towards 30 Sprague-Dawley rats. The sampling technique was convenience sampling. The sample was divided into two groups, the treatment group and the control group. The treatment group was administered with betadine plant sap, and the control group was administered with tranexamic acid orally using a gastric probe, then waited 4 hours after. The anaesthesia was then performed using 50 mg/kg body weight of ketamine intramuscularly. The extraction was performed after. Observations was conducted at the clinical criteria on days 1, 3, and 7 and continue to be monitored until the wound heals. Then, the signs of infection were observed. Data analysis was carried out using the Shapiro Wilk normality test. The results showed that the data was not normally distributed. Thus, data analysis was continued using the Mann Whitney statistical test. Results: The results showed a significant difference in the healing of socket wounds after tooth extraction after being administered with betadine sap and tranexamic acid with a value of p=0.037 (p<0.005). Conclusions: Administration of betadine plant sap is proven to be more effective in accelerating the healing process of socket wounds after tooth extraction than tranexamic acid. Keywords: Sprague-Dawley rats; wound healing; pencabutan gigi; getah batang betadine

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EFFECT OF WOUND CARE ON WOUND HEALING PROCESS OF POST OPERATION IN PATIENTS APENDEKTOMY IN RS DUSTIRA CIMAHI

Jurnal Persatuan Perawat Nasional Indonesia (JPPNI) ◽

10.32419/jppni.v4i1.60 ◽

2021 ◽

Vol 4 (1) ◽

Author(s):

Marlin Sutrisna ◽

Vega M Tusyanawati

Keyword(s):

Wound Healing ◽

Wound Care ◽

Healing Process ◽

Intervention Group ◽

Lower Abdominal Pain ◽

Control Group ◽

Wound Healing Process ◽

P Value ◽

Ablative Surgery ◽

Significant Difference

ABSTRACTVega M. Tusyanawati1, Marlin Sutrisna2, Tonika Tohri3 Sekolah Tinggi Ilmu Kesehatan Rajawali Bandung IndonesiaEmail : [email protected]: Appendicitis is one of the emergency cases in the abdominal area with a major complaint of persistent lower abdominal pain that develops and increased pain. An appendectomy is a surgical intervention that has the purpose of ablative surgery or removal of body parts that contain problems or have disease. One of the postoperative treatments of apendectomy is with wound care. The current wound care treatment is modern and conventional wound care. Objective: This study was to investigate the effect of treatment of postoperative wound healing apendectomy. Methodology: The design used in this research is Quasi Experiment, with posttest-only design approach. The number of samples taken is 18 respondent. Taking sampling with accidental sampling technique. Result: The result shows that p-value 0.001, with mean value in intervention group was 5.50 and in control group 13.50. Conclusion: There is a significant difference in the wound healing process using conventional and modern wound care.Keywords : type of wound care, post operative apendectomy

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COMPARISON OF THE EFFECTIVENESS OF GIVING BINAHONG LEAF (Anredera cordifolia (Ten) Steenis) AND PAPAYA LEAF (Carica papaya) ON SKIN WOUND HEALING IN WHITE RAT (Rattus novergicus)

Journal Widya Medika Junior ◽

10.33508/jwmj.v3i2.3181 ◽

2021 ◽

Vol 3 (2) ◽

pp. 78-85

Author(s):

Givenchy Winarjo ◽

◽

Fransiscus Arifin ◽

Dave Oenarta ◽

◽

...

Keyword(s):

Wound Healing ◽

Leaf Extract ◽

Healing Process ◽

Control Group ◽

Wound Healing Process ◽

P Value ◽

World Population ◽

White Rats ◽

Significant Difference ◽

The World

Introduction: The incidence of acute and chronic injuries increases every year. Most injuries in the world population, as many as 48%, are caused by trauma or surgery. the highest incidence of wounds in the world based on the etiology was from surgery, which was 110.30 million cases. One of the handlings of wounds on the skin can be done by giving herbal medicines, which can be easily accessed by the public, such as binahong leaves and papaya leaves. Purpose: To analyze the effectiveness of giving 40% binahong leaf extract cream and 40% papaya leaf extract cream on the wound distance in the white rat wound healing process. Method: this study is an experimental study with post-test only control group design approach. The sampling technique used in this study was a random sampling method where the number of samples was calculated using the Federer formula, with a total sample of 28 white rats. The distance of the wound was measured using a micrometer. Results: Twenty-eight wound distance on rats were measured as subjects of the study and then treated with binahong leaf extract cream and papaya leaf extract cream, then the wound distance of each treatment was measured using a micrometer. According to the results, 40% binahong leaf extract cream and 40% papaya leaf extract cream showed better effectiveness in reducing the wound distance of white rat incision than the negative control group (cream base) and positive control group (gentamicin ointment). According to the Mann Whitney test comparing the P1 group (40% binahong leaf extract cream) and P2 (40% papaya leaf extract cream), p-value >0.05, which is 0.543, which means that P1 has no significant mean difference with P2. Conclusion: There was no significant difference in effectiveness between the treatment of 40% binahong leaf extract cream and 40% papaya leaf extract cream.

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BINAHONG LEAVES (ANREDERA CORDIFOLIA TENORE STEEN) EXTRACT AS AN ALTERNATIVE TREATMENT FOR PERINEAL WOUND HEALING OF POSTPARTUM MOTHERS

Belitung Nursing Journal ◽

10.33546/bnj.290 ◽

2017 ◽

Vol 3 (6) ◽

pp. 778-783

Author(s):

Dita Selvia Aditia ◽

Syarief Thaufik Hidayat ◽

Nur Khafidhoh ◽

Suhartono Suhartono ◽

Ari Suwondo

Keyword(s):

Wound Healing ◽

Health Center ◽

Alternative Treatment ◽

Healing Process ◽

Perineal Wound ◽

Control Group ◽

Wound Healing Process ◽

P Value ◽

Perineal Wound Healing ◽

Postpartum Mothers

Background: Perineal wounds are at risk for infection, without a good treatment, perineal wound can harbor sepsis and become a chronic, long-term problem. Binahong leaves is considered as an alternative to accelerate perineal wound healing process.Objective: To determine the effect of binahong leaf extract (anredera cordifolia (tenore) steen) on perineal wound healing process in postpartum mothers.Methods: This study employed a quasy experimental study with control group posttest-only design. The study was conducted in the working area of Telogosari Wetan Health Center and Health Center of Kulon Kota Semarang in January-February 2017. There were 36 respondents selected using purposive sampling assigned in the intervention group (Binahong leaves extract) and the control group (Povidon Iodine 10%). REEDA scale was used for assessing perineal healing. Data were analyzed using Mann Whitney, Chi Square and Kruskall Wallis test.Results: Findings showed that the average day of perineal wound healing process in the binahong leaves group was 6.11 days and in the povidon iodine group was 7.66 days. There was a statistically significant difference in the duration of wound healing between the experiment and control group with p-value 0,001 (p value <0.05).Conclusion: Binahong leaves extract is effective in healing perineal wound in postpartum mothers. Therefore, binahong leaves are recommended to use as an alternative treatment for the mothers.

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Important Metabolites in Maintaining Folate Cycle, Homocysteine, and Polyamine Metabolism Associated with Ranibizumab Treatment in Cultured Human Tenon’s Fibroblasts

Biomolecules ◽

10.3390/biom9060243 ◽

2019 ◽

Vol 9 (6) ◽

pp. 243

Author(s):

Siti Munirah Md Noh ◽

Siti Hamimah Sheikh Abdul Kadir ◽

Sushil Vasudevan

Keyword(s):

Cell Cycle ◽

Wound Healing ◽

Healing Process ◽

Polyamine Metabolism ◽

Control Group ◽

Wound Healing Process ◽

P Value ◽

Nucleotide Synthesis ◽

Folate Cycle ◽

Ranibizumab Treatment

The anti-fibrotic properties of ranibizumab have been well documented. As an antagonist to vascular endothelial growth factor (VEGF), ranibizumab works by binding and neutralizing all active VEGF-A, thus limiting progressive cell growth and proliferation. Ranibizumab application in ocular diseases has shown remarkable desired effects; however, to date, its antifibrotic mechanism is not well understood. In this study, we identified metabolic changes in ranibizumab-treated human Tenon’s fibroblasts (HTFs). Cultured HTFs were treated for 48 h with 0.5 mg/mL of ranibizumab and 0.5 mg/mL control IgG antibody which serves as a negative control. Samples from each group were injected into Agilent 6520 Q-TOF liquid chromatography/mass spectrometer (LC/MS) system to establish the metabolite expression in both ranibizumab treated cells and control group. Data obtained was analyzed using Agilent Mass Hunter Qualitative Analysis software to identify the most regulated metabolite following ranibizumab treatment. At p-value < 0.01 with the cut off value of two-fold change, 31 identified metabolites were found to be significantly upregulated in ranibizumab-treated group, with six of the mostly upregulated having insignificant role in fibroblast cell cycle and wound healing regulations. Meanwhile, 121 identified metabolites that were downregulated, and seven of the mostly downregulated are significantly involved in cell cycle and proliferation. Our findings suggest that ranibizumab abrogates the tissue scarring and wound healing process by regulating the expression of metabolites associated with fibrotic activity. In particular, we found that vitamin Bs are important in maintaining normal folate cycle, nucleotide synthesis, and homocysteine and spermidine metabolism. This study provides an insight into ranibizumab’s mechanism of action in HTFs from the perspective of metabolomics.

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BREAST MILK AS AN ALTERNATIVE FOR POSTPARTUM PERINEAL CARE

Belitung Nursing Journal ◽

10.33546/bnj.101 ◽

2017 ◽

Vol 3 (3) ◽

pp. 238-245 ◽

Cited By ~ 1

Author(s):

Yuli Admasari ◽

Bedjo Santoso ◽

Titi Suherni ◽

Imam Djamaluddin Mashoedi ◽

Mardiyono Mardiyono

Keyword(s):

Wound Healing ◽

Breast Milk ◽

Health Workers ◽

Healing Process ◽

Intervention Group ◽

Perineal Wound ◽

Control Group ◽

Wound Healing Process ◽

P Value ◽

Perineal Laceration

Background: Perineal laceration during childbirth is very common among mothers; however some of them may suffer from its complication if not treated properly.Objective: To prove the effectiveness of breast milk as an alternative topical ingredient in the treatment of perineal wound in postpartum mothers.Methods: This was a quasi-experimental study with non-equivalent control group posttest only design. There were 30 respondents selected in this study, with 15 assigned in the intervention and control group. Accidental sampling was used to select the samples with the criteria that the respondents had perineal laceration in level 1 and 2. Data were analzed using Mann Whitney test.Results: Effective wound healing process can be seen in the intervention group from 80% of poor category in 6-10 hours (1st period) of postpartum became 86.7% of good category in 7 days of postpartum (4th period). Different from the control group that showed the slow progress of wound healing, which was 86.7% of poor category in the 1st period to only 33.3% of good category in the 4th period. Mann Whitney test showed that there was a significant mean difference of the perineal wound healing process between the intervention group (11.23) and the control group (19.77) with p-value 0.002 (<0.05).Conclusion: Breast milk was more effective than povidone iodine in the treatment of perineal wound. It is suggested for health workers, especially midwife to apply this intervention to accelerate the healing of perineal wound in midwifery care.

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Pengaruh Aplikasi Gel Ekstrak Kulit Citrus Sinensis terhadap Epitelisasi pada Penyembuhan Luka Gingiva Tikus Sprague Dawley

Majalah Kedokteran Gigi Indonesia ◽

10.22146/majkedgiind.9012 ◽

2015 ◽

Vol 1 (1) ◽

pp. 86

Author(s):

Aqilla Tiara Kartiningtyas ◽

Prayitno Prayitno ◽

Sri Pramestri Lastianny

Keyword(s):

Wound Healing ◽

Sweet Orange ◽

Healing Process ◽

Orange Peel ◽

Control Group ◽

Wound Healing Process ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Orange Peel Extract ◽

Peel Extract

Penyembuhan luka merupakan mekanisme pertahanan jaringan, dengan epitelisasi sebagai salah satu parameternya. Kulit jeruk manis memiliki potensi dalam penyembuhan luka karena mengandung nutrisi yang memiliki peran dalam mempercepat penyembuhan luka. Penelitian ini bertujuan untuk mengetahui efek aplikasi topikal gel ekstrak kulit jerukmanis terhadap epitelisasi pada proses penyembuhan luka gingiva labial tikus Sprague Dawley. Dua puluh tujuh ekor tikus Sprague Dawley, berumur 2-3 bulan, dibagi dalam 3 kelompok, kontrol positif, perlakuan, dan kontrol negatif. Perlukaan pada gingiva labial mandibula dibuat dengan punch biopsy berdiameter 2,5 mm. Luka pada kelompok kontrolpositif diaplikasikan Aloclair, kelompok perlakuan diaplikasikan gel ekstrak kulit jeruk manis 10%, dan kelompok kontrol negatif diaplikasikan CMC-Na, masing-masing 2 kali sehari selama 1 menit secara topikal. Tiga ekor tikus dari tiap kelompok didekapitasi masing-masing pada hari ke-3, 7, dan 14. Jaringan luka diambil dan dibuat sediaan histologis dengan pengecatan Hemaktosilin Eosin. Pengukuran ketebalan epitel dilakukan dengan menggunakan Optilab yang dipasang pada mikroskop cahaya. Data ketebalan epitel dianalisis dengan menggunakan ANAVA dua jalur. Hasil pengukuran ketebalan epitel menunjukkan perbedaan yang tidak signifikan pada kelompok kontrol positif dengan perlakuan (p>0,05), namun menunjukkan perbedaan yang signifikan pada kelompok perlakuan dengan kontrol negatif (p<0,05) pada hari 3, 7, dan 14 setelah perlukaan. Kesimpulan dari penelitian ini adalah aplikasi topikal gel ekstrak kulit jeruk manis mempercepat epitelisasi pada proses penyembuhan luka gingiva labial tikus Sprague Dawley. Effect of Topical Application of Sweet Orange (Citrus sinensis) Peel Extract Gel on Epithelialization of Labial Gingival Wound Healing: In Vivo Studies in Sprague Dawley Rats. Wound healing is a defense mechanism from complex biological phenomenon, in which epithelialization occurs as one of its parameters. Sweet orange peelcontains nutrients that have role in enhancing wound healing process. The objective of this research is to determine the effect of topical application of sweet orange peel extract gel on epithelialization of wound healing process in labial mandibular gingiva of Sprague Dawley rats. Twenty seven Sprague Dawley rats were divided into 3 groups: positivecontrol, treatment, and negative control. Labial mandibular gingival was wounded using 2.5 mm diameter punch biopsy. Topically, each wound of positive control group was administered Aloclair, treatment group was administered 10% sweet orange peel extract gel, and negative control group was administered CMC-Na, twice a day for 1 minute. Three rats from each group were sacrificed for histological evaluation at 3, 7, and 14 days and the specimens were stained with HE. The measurement of the epithelial thickness used Optilab installed on microscope. The data obtained from the measurement was analyzed using two-way ANOVA. There was insignificant difference between positive control group and treatment group (p>0.05) while there was significant difference between treatment group and negative control group (p<0.05) observed at 3, 7, and 14 days. The conclusion of this research is that the application of sweet orange peel extract gel accelerates epithelialization of wound healing process in labial mandibular gingiva of Sprague Dawley rats.

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The Effect of Honey Compress Therapy Toward Skin Wound Healing for Full Thicknes Loss on Rattus Norvegicus

Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery) ◽

10.26699/jnk.v2i2.art.p103-107 ◽

2015 ◽

Vol 2 (2) ◽

pp. 103-107

Author(s):

Dina Zakiyyatul Fuadah ◽

Diana Rachmania ◽

Novita Yudik

Keyword(s):

Wound Healing ◽

Healing Process ◽

Skin Wound ◽

Control Group ◽

Wound Healing Process ◽

P Value ◽

Skin Wound Healing ◽

Control Group Design ◽

Post Test ◽

Skin Loss

Wounds require a very effective treatment for example an open wound. Honey compress therapy is one method that can be used for wound healing process. The purpose of this study, to determine the effect of therapy on wound healing using honey compress full thicknes skin loss on Rattus norvegicus.Design for the research is True Experiments using post-test design with control group design. Samples were 18. Sampling using random sampling techniques. The independent variable is the honey compress therapy and the dependent variable is the process of wound healing of full thicknes skin loss. Data were analyzed using the Mann Whitney test (α = 0.05).The results of the statistical test on day 15, p value = 0.004 <α that’s meanthere are significant differences between the treatment group and the control group in wound healing of full thicknes skin loss on Rattus norvegicus.Honey compress therapy accelerates wound healing process full thicknes skin loss. It is recommended for people can take advantage of pure honey for wound healing due to the composition of honey that can help the healing process.

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Expression of fibroblast growth factor-2 after application of the Queen's crepe-myrtle leaf (Lagerstroemia speciosa) and aloe vera extract gel in the wound healing process of hyperglycemic

Padjadjaran Journal of Dentistry ◽

10.24198/pjd.vol33no1.21276 ◽

2021 ◽

Vol 33 (1) ◽

pp. 48

Author(s):

Fachrul Razi ◽

Andri Hardianto ◽

Lucky Riawan ◽

Bambang Pontjo Priosoeryanto

Keyword(s):

Wound Healing ◽

Healing Process ◽

Aloe Vera ◽

Simple Random Sampling ◽

Fibroblast Growth Factor 2 ◽

Control Group ◽

Wound Healing Process ◽

Sprague Dawley ◽

Lagerstroemia Speciosa ◽

Significant Difference

Introduction: Fibroblast Growth Factor-2 (FGF-2) have a role in stimulating the proliferation and migration of various types of cells in the wound healing process. Hyperglycemic conditions can disrupt the wound healing process. Lagerstroemia speciosa are known to have antyphyerglycemic and antioxidant effects. Aloe vera is a plant that has been used for long time in topical treatment of wounds.This study was aimed to analyse the FGF-2 expressions on the wound healing process of subjects with hyperglycemia after application of Queen's crepe-myrtle leaf (Lagerstroemia speciosa) and aloe vera extracts gel. Methods: A pure experimental study was conducted with simple random sampling. The sample was 27 experimental animals (CI 95%; power test 80%) of Sprague Dawley rat induced to have a hyperglycemic state using alloxan and had their palate injured. The immunohistochemical examination was performed to assess FGF-2 expression level. Samples were divided into three groups with a simple random sampling technique: nine of the rats received the Lagerstroemia speciosa extract gel application; nine rats received the aloe vera extract gel application on the injured palate; another nine rats in the control group. Examination of the FGF-2 expression level was performed on the third, seventh, and fourteenth observation days. Results: Overall, the control group had a significant difference with the Lagerstroemia speciosa and aloe vera extract gel group (p-value<0.05), however, the Lagerstroemia speciosa group did not have any significant difference with the aloe vera group (0.123>0.05). Conclusion: There was no difference in the amount of FGF-2 expressions on the wound healing process of the injured palatal tissue of Sprague-Dawley rats with hyperglycemic after application of the Lagerstroemia speciosa and aloe vera extract gel.

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EFEK EKSTRAK TANAMAN ASHITABA PADA KADAR SERUM ALKALIN FOSFATASE (ALP) PADA TIKUS YANG TERPAPAR LUKA BAKAR

JIFI (Jurnal Ilmiah Farmasi Imelda) ◽

10.52943/jifarmasi.v5i1.662 ◽

2021 ◽

Vol 5 (1) ◽

pp. 26-34

Author(s):

Andrew Johan ◽

Kusmiyati Tjahjono ◽

Wenda Yoanda ◽

Dwi Ngestiningsih ◽

Lusiana Batubara

Keyword(s):

Wound Healing ◽

Treatment Group ◽

Serum Alkaline Phosphatase ◽

Healing Process ◽

T Test ◽

Control Group ◽

Wound Healing Process ◽

Sprague Dawley ◽

Burn Wound ◽

Control Group Design

Serum alkaline phosphatase (ALP) levels increases in burn tissue damage. Several bioactive compounds found in Ashitaba, are expected to reduce serum ALP levels and enhance the process of wound healing. This study aims to prove that administration of oral Ashitaba extract can reduce serum ALP levels in rats exposed to burn trauma. This study uses true experimental post-test control group design with a total of 20 Sprague dawley rats as samples. All samples were inflicted with 2nd degree burn wound and divided into 2 groups, treatment group (Ashitaba extract 300 mg/KgBW) and control group. Blood serum were analyzed for ALP levels on the 2nd, 8th and 14th days. Kinetic-IFCC method was used to find serum ALP levels. Data was analyzed using Mann-Whitney Test, paired T-test amd independence T-test. Scalded wound size was measured macroscopically over the course of 21 days to find contracture rate. In conclusion, Ashitaba extract is not proven to significantly reduce the serum ALP levels, increase contracture rate and enhance burn wound healing process. However, there was slight increase in contracture rate in treatment group as compared to control group. In addition, there was a lower ALP levels in treatment group as compared to control group in day 8.

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