scholarly journals Isoform-Specific Role of Akt in Oral Squamous Cell Carcinoma

Biomolecules ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 253 ◽  
Author(s):  
Roy ◽  
Monisha ◽  
Padmavathi ◽  
Lalhruaitluanga ◽  
Kumar ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cell Survival ◽  
Cancer Cells ◽  
B Cell Lymphoma ◽  
Genetic Alterations ◽  
Specific Role ◽  
Migration Potential ◽  
Oral Cancer Cells ◽  
And Migration

Protein kinase B (Akt) plays a very significant role in various cancers including oral cancer. However, it has three isoforms (Akt1, Akt2, and Akt3) and they perform distinct functions and even play contrasting roles in different cancers. Therefore, it becomes essential to evaluate the isoform-specific role of Akt in oral cancer. In the present study, an attempt has been made to elucidate the isoform-specific role of Akt in oral cancer. The immunohistochemical analysis of oral cancer tissues showed an overexpression of Akt1 and 2 isoforms but not Akt3. Moreover, the dataset of “The Cancer Genome Atlas” for head and neck cancer has suggested the genetic alterations of Akt1 and 2 tend to be associated with the utmost poor clinical outcome in oral cancer. Further, treatment of oral cancer cells with tobacco and its components such as benzo(a)pyrene and nicotine caused increased mRNA levels of Akt1 and 2 isoforms and also enhanced the aggressiveness of oral cancer cells in terms of proliferation, and clonogenic and migration potential. Finally, silencing of Akt1 and 2 isoforms caused decreased cell survival and induced cell cycle arrest at the G2/M phase. Akt1/2 silencing also reduced tobacco-induced aggressiveness by decreasing the clonogenic and migration potential of oral cancer cells. Moreover, silencing of Akt1 and 2 isoforms was found to decrease the expression of proteins regulating cancer cell survival and proliferation such as cyclooxygenase-2, B-cell lymphoma 2 (Bcl-2), cyclin D1, and survivin. Thus, the important role of Akt1 and 2 isoforms have been elucidated in oral cancer with in-depth mechanistic analysis.

scholarly journals Efficient Delivery of Therapeutic siRNA by Fe3O4 Magnetic Nanoparticles into Oral Cancer Cells

Pharmaceutics ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 615 ◽  
Author(s):  
Lili Jin ◽  
Qiuyu Wang ◽  
Jiayu Chen ◽  
Zixiang Wang ◽  
Hongchuan Xin ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cancer Cells ◽  
B Cell Lymphoma ◽  
Cell Counting ◽  
Blue Staining ◽  
Dependent Manner ◽  
Electronic Microscopy ◽  
Concentration Dependent Manner ◽  
Efficient Delivery ◽  
Oral Cancer Cells

The incidence of oral cancer is increasing due to smoking, drinking, and human papillomavirus (HPV) infection, while the current treatments are not satisfactory. Small interfering RNA (siRNA)-based therapy has brought hope, but an efficient delivery system is still needed. Here, polyethyleneimine (PEI)-modified magnetic Fe3O4 nanoparticles were prepared for the delivery of therapeutic siRNAs targeting B-cell lymphoma-2 (BCL2) and Baculoviral IAP repeat-containing 5 (BIRC5) into Ca9-22 oral cancer cells. The cationic nanoparticles were characterized by transmission electronic microscopy (TEM), scanning electronic microscopy (SEM), dynamic light scattering (DLS), and vibrating sample magnetometer (VSM). By gel retardation assay, the nanoparticles were found to block siRNA in a concentration-dependent manner. The cellular uptake of the nanoparticle/siRNA complexes under a magnetic field was visualized by Perl’s Prussian blue staining and FAM labeling. High gene silencing efficiencies were determined by quantitative real-time PCR and western blotting. Furthermore, the nanoparticle-delivered siRNAs targeting BCL2 and BIRC5 were found to remarkably inhibit the viability and migration of Ca9-22 cells, by cell counting kit-8 assay and transwell assay. In this study, we have developed a novel siRNA-based therapeutic strategy targeting BCL2 and BIRC5 for oral cancer.

scholarly journals Tapirira guianensis Aubl. Extracts Inhibit Proliferation and Migration of Oral Cancer Cells Lines

2016 ◽  
Vol 17 (11) ◽  
pp. 1839 ◽  
Author(s):  
Renato Silva-Oliveira ◽  
Gabriela Lopes ◽  
Luiz Camargos ◽  
Ana Ribeiro ◽  
Fábio Santos ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cancer Cells ◽  
Oral Cancer Cells ◽  
And Migration ◽  
Proliferation And Migration

Gypenosides inhibits migration and invasion of human oral cancer SAS cells through the inhibition of matrix metalloproteinase-2 -9 and urokinase-plasminogen by ERK1/2 and NF-kappa B signaling pathways

2010 ◽  
Vol 30 (5) ◽  
pp. 406-415 ◽  
Author(s):  
Kung-Wen Lu ◽  
Jung-Chou Chen ◽  
Tung-Yuan Lai ◽  
Jai-Sing Yang ◽  
Shu-Wen Weng ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Matrix Metalloproteinase ◽  
Cancer Cells ◽  
Time Dependent ◽  
Migration And Invasion ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Invasion And Migration ◽  
Oral Cancer Cells ◽  
And Migration

Gypenosides (Gyp), found in Gynostemma pentaphyllum Makino, has been used as a folk medicine in the Chinese population for centuries and is known to have diverse pharmacologic effects, including anti-proliferative and anti-cancer actions. However, the effects of Gyp on prevention from invasion and migration of oral cancer cells are still unsatisfactory. The purpose of this study was to investigate effects of Gyp treatment on migration and invasion of SAS human oral cancer cells. SAS cells were cultured in the presence of 90 and 180 μg/mL Gyp for 24 and 48 hours. Gyp induced cytotoxic effects and inhibited SAS cells migration and invasion in dose- and time-dependent response. Wound-healing assay and boyden chamber assay were carried out to investigate Gyp-inhibited migration and invasion of SAS cells. Gyp decreased the abundance of several proteins, including nuclear factor-kappa B (NF-κB), cyclooxygenase-2 (COX-2), extracellular signal-regulated kinase 1/2 (ERK1/ 2), matrix metalloproteinase-9, -2 (MMP-9, -2), sevenless homolog (SOS), Ras, urokinase-type plasminogen activator (uPA), focal adhesion kinase (FAK) and RAC-alpha serine/threonine-protein kinase (Akt), in a time-dependent manner. In addition, Gyp decreased mRNA levels of MMP-2, MMP-7, MMP-9 but did not affect FAK and Rho A mRNA levels in SAS cells. These results provide evidences for the role of Gyp as a potent anti-metastatic agent, which can markedly inhibit the metastatic and invasive capacity of oral cancer cells. The inhibition of NF-κB and MMP-2, -7 and -9 signaling may be one of the mechanisms that is present in Gyp-inhibited cancer cell invasion and migration.

P3.31. Role of GSK-3β as a negative regulator of epithelial-mesenchymal transition in oral cancer cells

2009 ◽  
Vol 3 (1) ◽  
pp. 210-211
Author(s):  
J.H. Kang ◽  
J.J. Joung ◽  
J.B. Cho ◽  
P.Y. Yun ◽  
J.H. Lee ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cancer Cells ◽  
Epithelial Mesenchymal Transition ◽  
Negative Regulator ◽  
Mesenchymal Transition ◽  
Oral Cancer Cells ◽  
Gsk 3Β

CD147 promotes proliferation and migration of oral cancer cells by inhibiting junctions between E‐cadherin and β‐catenin

2020 ◽  
Vol 49 (10) ◽  
pp. 1019-1029
Author(s):  
Anjie Min ◽  
Haofeng Xiong ◽  
Weiming Wang ◽  
Xin Hu ◽  
Can Wang ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cancer Cells ◽  
Oral Cancer Cells ◽  
And Migration ◽  
Proliferation And Migration ◽  
E Cadherin

scholarly journals circVAPA promotes the proliferation, migration and invasion of oral cancer cells through the miR-132/HOXA7 axis

2021 ◽  
Vol 49 (6) ◽  
pp. 030006052110132
Author(s):  
Hao Chen ◽  
Ye Zhang ◽  
Kankui Wu ◽  
Xiaoyong Qiu
Keyword(s):  
Oral Cancer ◽  
Cancer Cells ◽  
Cell Counting ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Loss Of Function ◽  
Invasion And Migration ◽  
Oral Cancer Cells ◽  
And Migration

Objective To study the relationship between the circular RNA vesicle-associated membrane protein-associated protein A (circVAPA) and the pathogenesis of oral squamous cell carcinoma. Methods The expression of circVAPA was detected by RT-qPCR. In vitro loss-of-function experiments were performed in Cal-27 cells. The malignant phenotype of cells was evaluated by cell counting kit-8, clone formation and transwell assays. Luciferase reporter assays were used to assess the circVAPA/miR-132/homeobox A (HOXA) regulatory axis. Results circVAPA expression was significantly increased in oral cancer tissues and cells. The overall survival and progression-free survival of patients with oral cancer who exhibited high circVAPA expression were significantly shorter compared with those with low expression. circVAPA expression was closely related to tumor size, TNM stage and distant metastasis. circVAPA knockdown reduced the proliferation, invasion and migration of Cal-27 cells. MiR-132 was identified as a target of circVAPA in Cal-27 cells. Cotransfection with si-circVAPA and miR-132 inhibitor reversed the inhibitory effect of circVAPA knockdown on cell malignant phenotypes. HOXA7 was further identified as a downstream target of miR-132. Conclusion circVAPA is highly expressed in oral cancer, and its abnormal expression might affect the proliferation, invasion and migration of oral cancer cells by modulating the miR-132/HOXA7 signaling axis.

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