scholarly journals Combining Electrostatic, Hindrance and Diffusive Effects for Predicting Particle Transport and Separation Efficiency in Deterministic Lateral Displacement Microfluidic Devices

Biosensors ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 126
Author(s):  
Valentina Biagioni ◽  
Giulia Balestrieri ◽  
Alessandra Adrover ◽  
Stefano Cerbelli
Keyword(s):  
Particle Transport ◽  
Separation Efficiency ◽  
Lateral Displacement ◽  
Operating Conditions ◽  
Separation Performance ◽  
Label Free ◽  
Deterministic Lateral Displacement ◽  
Promising Tool ◽  
Label Free Detection ◽  
Diffusive Effects

Microfluidic separators based on Deterministic Lateral Displacement (DLD) constitute a promising technique for the label-free detection and separation of mesoscopic objects of biological interest, ranging from cells to exosomes. Owing to the simultaneous presence of different forces contributing to particle motion, a feasible theoretical approach for interpreting and anticipating the performance of DLD devices is yet to be developed. By combining the results of a recent study on electrostatic effects in DLD devices with an advection–diffusion model previously developed by our group, we here propose a fully predictive approach (i.e., ideally devoid of adjustable parameters) that includes the main physically relevant effects governing particle transport on the one hand, and that is amenable to numerical treatment at affordable computational expenses on the other. The approach proposed, based on ensemble statistics of stochastic particle trajectories, is validated by comparing/contrasting model predictions to available experimental data encompassing different particle dimensions. The comparison suggests that at low/moderate values of the flowrate the approach can yield an accurate prediction of the separation performance, thus making it a promising tool for designing device geometries and operating conditions in nanoscale applications of the DLD technique.

scholarly journals Microfluidic label-free bioprocessing of human reticulocytes from erythroid culture

Lab on a Chip ◽  
2020 ◽  
Vol 20 (18) ◽  
pp. 3445-3460
Author(s):  
Kerwin Kwek Zeming ◽  
Yuko Sato ◽  
Lu Yin ◽  
Nai-Jia Huang ◽  
Lan Hiong Wong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Closed Loop ◽  
Lateral Displacement ◽  
Label Free ◽  
Hematopoietic Stem ◽  
Deterministic Lateral Displacement ◽  
Dean Flow ◽  
Flow Fractionation ◽  
Human Rbcs

Developments in Dean flow fractionation (DFF) and deterministic lateral displacement (DLD) for label-free purification of cultured RBCs from human hematopoietic stem cells. An advancement in sorting and closed-loop manufacturing of viable human RBCs.

Multiphase CFD Model Development for a Rotating Centrifugal Separator

2012 ◽  
Author(s):  
Daniel DeMore ◽  
William Maier
Keyword(s):  
Separation Efficiency ◽  
Fluid Dynamic ◽  
Model Development ◽  
Modeling Method ◽  
Operating Conditions ◽  
Cfd Modeling ◽  
Separation Performance ◽  
Centrifugal Separator ◽  
Wide Range ◽  
The Impact

The present paper describes the development of a Computational Fluid Dynamic (CFD) modeling approach suitable for the analysis, design, and optimization of rotating centrifugal separator stage geometries. The Homogeneous Multiple Size Group (MUSIG) model implemented in the commercial code CFX V13.0 was utilized as a basis for the CFD modeling method. The model was developed through a series of studies to understand the impact of droplet size distribution, particle coalescence, rotor/stator interface treatment, and mesh resolution on the prediction of separation efficiency for a given rotating separator geometry. This model was then validated against the OEM’s extensive in-house experimental separation testing database. The resulting CFD modeling method is shown to adequately reproduce observed trends in separation performance over a wide range of operating conditions.

scholarly journals Numerical Study on the Influence of Length-Diameter Ratio on the Performance of Dynamic Pressure Oil-Air Separator

2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
Xiaobin Zhang ◽  
Xiaofeng Zhang ◽  
Delin Gu ◽  
Lei Lang ◽  
Na Gao
Keyword(s):  
Aspect Ratio ◽  
Separation Efficiency ◽  
Dynamic Pressure ◽  
Diameter Ratio ◽  
Operating Conditions ◽  
Maximum Efficiency ◽  
Separation Performance ◽  
Cylinder Length ◽  
Cylinder Diameter ◽  
Air Separator

In order to study the separation characteristics of the aeroengine dynamic pressure oil-air separator, this paper uses the coupling method of PBM and CFD two-fluid model to study the influencing factors such as cylinder diameter, cylinder length, and other factors on the separator performance. The flow field structure, velocity, gas volume distribution, separation efficiency, and gas and liquid holdup rate in the separator under different operating conditions are analyzed. Combined with the analysis results of the cylinder diameter and the cylinder length, the influence law of length-diameter ratio on separation efficiency is summarized. The optimum length-to-diameter ratio that maximizes the separation performance of the separator is obtained in this research, which provides a reference for the design and improvement of the separator. The results show that, as the diameter of the cylinder increases, the separation efficiency increases first and then decreases. When dsep = 16 mm and dsep = 18 mm, the separator reaches its maximum efficiency, which is about 93%. With the increase of the cylinder length, the separation efficiency first increases and reaches the maximum when l2 = 90 mm and then decreases slowly. When the separator cylinder is either too long or too short, it will cause the separation performance to decrease. There is an optimal aspect ratio. There is an optimal aspect ratio, and the separation performance of the separator is the best when the aspect ratio is between 5 and 6.

Dry beneficiation of +0.5mm-5.6mm South African coal using an air dense medium fluidization bed

2021 ◽  
Author(s):  
Elmarie Sunette Diedericks ◽  
Marco Le Roux ◽  
Quentin Peter Campbell
Keyword(s):  
Particle Size ◽  
Fluidized Bed ◽  
Separation Efficiency ◽  
Solid Phase ◽  
Poor Performance ◽  
Operating Conditions ◽  
High Mass ◽  
Dense Medium ◽  
Separation Performance ◽  
Coal Particles

Abstract The separation performance of solid phase bed material, at various particle size ranges, in an air dense medium fluidized bed (ADMFB), were evaluated during this study. The coal particles were separated into +0.5mm-1mm, +1mm-2mm, +2mm-2.8mm, +2.8mm-4mm, +4mm-4.75mm and +4.75mm-5.6mm particle size ranges and fed to the fluidized bed in these fractions. Along with the six coal particle size ranges, three dense media to coal ratios and the addition of vibration was tested to identify the best operating conditions. Adequate results were obtained for larger particle size ranges down to and including +2.0mm-2.8mm coal particles, after which the separation performance decreased significantly. Density stratification was irregular and not obvious for coal particles below 2.0mm and maintaining a consistent fluidization state also proved to be challenging, especially when dense medium was added. The coal particles separated vertically along the bed height because of differences in particle and bed density, while particle size proved to have a notable influence on the degree of separation. An air fluidization velocity of between 1.1 to 1.4Umf was shown as the best performing velocity, which yielded the maximum ash differential between the top and bottom layers of the bed for all the particle size ranges tested. For +2.0mm-5.6mm coal particles, low cumulative ash yields were obtained at high mass yields, however the ash yields increased for -2mm coal. Vibration and dense medium have, in some cases, enhanced the separation efficiency of the ADMFB. The -2.0mm particles experienced stronger particle-particle interactions as well as elevated levels of bubbling and back mixing than that of the +2.0mm particles, which explains the poor performance of the small particle sizes.

scholarly journals An Experimental Study on Performance and Structural Improvements of a Novel Elutriator

Processes ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 478
Author(s):  
Jipeng Dong ◽  
Pan Zhang ◽  
Weiwen Wang ◽  
Jianlong Li ◽  
Guanghui Chen
Keyword(s):  
Gas Flow ◽  
Fine Particles ◽  
Separation Efficiency ◽  
Particle Dispersion ◽  
Operating Conditions ◽  
Separation Performance ◽  
Fine Dust ◽  
Fire Hazards ◽  
Feeding Speed ◽  
Air Speed

During the transportation and packaging of low density polyethylene (LDPE) granular materials, fine dusts such as floccules, powder and fiber will be produced, which pollute the environment, affect product quality and generate fire hazards. In this work, the separation performance of fine dust and optimal operating conditions of an improved elutriator were investigated experimentally. Experiments were carried out to investigate the effects of air speed, feeding speed, and grid layout on the removal efficiency of fine particles. Experimental data showed that the separation efficiency of the novel elutriator ranged from 96% to 98.50%, which was more stable and an average of 51.44% higher than that of the original elutriator. By setting internals and improving the structure, the gas flow field in the equipment was regulated, the particle dispersion was intensified, and the static electricity was eliminated, which significantly improved the separation efficiency of fine dust.

scholarly journals Linker histone H1 determines cell stiffness and differentiation

2020 ◽  
Author(s):  
Marie Kijima ◽  
Hiroyuki Yamagishi ◽  
Riho Kawano ◽  
Tomoki Konishi ◽  
Takuya Okumura ◽  
...  
Keyword(s):  
Lateral Displacement ◽  
Histone H1 ◽  
Cell Stiffness ◽  
Label Free ◽  
Size Fractionation ◽  
Mechanical Stiffness ◽  
Deterministic Lateral Displacement ◽  
Linear Relationships ◽  
Nuclear Changes ◽  
Positively Charged

ABSTRACTThe flexibility or stiffness, one mechanical property of cells, is a promising label-free biomarker for underlying cytoskeletal or nuclear changes associated with various disease processes and changes in cell state. However, the molecular changes responsible for the whole-cell mechanical stiffness remain to be clarified. Recently, it was shown that the deterministic lateral displacement (DLD) microfluidic device, originally developed for size fractionation of some particles, might be applied to distinguish cells according to their stiffness. In this experiment, using the DLD device and various cell lines differentially expressing histone H1, a positively-charged protein localized in the linker region of chromatin, we found linear relationships between histone H1 quantity and cell stiffness. We also found that the histone H1 quantity affected cell size and even cell differentiation.

A New Method for the Label-Free Detection of Vascular Endothelial Growth Factor Based on DNAzymes

2016 ◽  
Vol 69 (1) ◽  
pp. 85
Author(s):  
Zhuwu Lv ◽  
Ke Wang ◽  
Xiaolu Zhang
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Patient Survival ◽  
Treatment Success ◽  
Survival Rates ◽  
Vascular Endothelial ◽  
Label Free ◽  
Promising Tool ◽  
Label Free Detection ◽  
Endothelial Growth Factor

The possibility of performing reliable cancer diagnosis even before any symptoms of disease appears is crucial for increasing therapeutic treatment success and patient survival rates. Herein, an ultrasensitive colorimetric sandwich sensor for vascular endothelial growth factor (VEGF) detection is introduced. The peroxidase-like DNAzyme catalyzes the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid, which generates a blue–green colorimetric signal. This method can detect VEGF at a concentration as low as 10 nM. This strategy will be a promising tool for bioanalytical and clinical applications.

scholarly journals On the Three-Dimensional Structure of the Flow through Deterministic Lateral Displacement Devices and Its Effects on Particle Separation

Processes ◽  
2019 ◽  
Vol 7 (8) ◽  
pp. 498 ◽  
Author(s):  
Valentina Biagioni ◽  
Alessandra Adrover ◽  
Stefano Cerbelli
Keyword(s):  
Lateral Displacement ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Periodic Lattice ◽  
Separation Mechanism ◽  
Label Free ◽  
Deterministic Lateral Displacement ◽  
Slip Conditions ◽  
Flow Through ◽  
The Impact

Experiments have shown that a suspension of particles of different dimensions pushed through a periodic lattice of micrometric obstacles can be sorted based on particle size. This label-free separation mechanism, referred to as Deterministic Lateral Displacement (DLD), has been explained hinging on the structure of the 2D solution of the Stokes flow through the patterned geometry, thus neglecting the influence of the no-slip conditions at the top and bottom walls of the channel hosting the obstacle lattice. We show that the no-slip conditions at these surfaces trigger the onset of off-plane velocity components, which impart full three-dimensional character to the flow. The impact of the 3D flow structure on particle transport is investigated by enforcing an excluded volume approach for modelling the interaction between the finite-sized particles and the solid surfaces. We find that the combined action of particle diffusion and of the off-plane velocity component causes the suspended particles to migrate towards the top and bottom walls of the channel. Preliminary results suggest that this effect makes the migration angle of the particles significantly different from that obtained by assuming a strictly two-dimensional structure for the flow of the suspending fluid.

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