Electrospun Fibres of Chitosan/PVP for the Effective Chemotherapeutic Drug Delivery of 5-Fluorouracil

Electrospun nanofibrous mats consisting of chitosan (CS) and polyvinylpyrrolidone (PVP) were constructed. Tuning of solution and process parameters was performed and resulted in an electrospun system containing a 6:4 ratio of PVP:CS. This is a significant increase in the proportion of spun CS on the previously reported highest ratio PVP:CS blend. SEM analysis showed that the nanofibrous mats with 4 wt% CS/6 wt% PVP (sample E) comprised homogenous, uniform fibres with an average diameter of 0.569 μm. XPS analysis showed that the surface of the samples consisted of PVP. Raman and FTIR analysis revealed intermolecular interactions (via H-bonding) between PVP and CS. In FTIR spectra, the contribution of chitosan to CS/PVP complexes was shown by the downshift of the C=O band and by the linear increase in intensity of C-O stretching in CS. XPS analysis showed a smaller shift at the binding energy 531 eV, which relates to the amide of the acetylated functional groups. The obtained results demonstrate a sensitivity of Raman and FTIR tests to the presence of chitosan in PVP:CS blend. The chemotherapy drug 5-Fu was incorporated into the constructs and cell viability studies were performed. WST-8 viability assay showed that exposure of A549 human alveolar basal epithelial cells to 10 mg/mL 5-Fu loaded fibres was most effective at killing cells over 24 h. On the other hand, the constructs with loading of 1 mg/mL of drug were not efficient at killing A549 human alveolar basal epithelial cells. This study showed that CS/PVP/5-Fu constructs have potential in chemotherapeutic drug delivery systems.

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Quantum dots as targeted doxorubicin drug delivery nanosystems

Cancer Nanotechnology ◽

10.1186/s12645-021-00077-9 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Monika Ruzycka-Ayoush ◽

Patrycja Kowalik ◽

Agata Kowalczyk ◽

Piotr Bujak ◽

Anna M. Nowicka ◽

...

Keyword(s):

Lung Cancer ◽

Drug Delivery ◽

Quantum Dots ◽

Epithelial Cells ◽

A549 Cells ◽

Dna Breaks ◽

3T3 Cells ◽

Nih 3T3 ◽

Nih 3T3 Cells ◽

Basal Epithelial Cells

Abstract Background Lung cancer is one of the most frequently diagnosed cancers all over the world and is also one of the leading causes of cancer-related mortality. The main treatment option for small cell lung cancer, conventional chemotherapy, is characterized by a lack of specificity, resulting in severe adverse effects. Therefore, this study aimed at developing a new targeted drug delivery (TDD) system based on Ag–In–Zn–S quantum dots (QDs). For this purpose, the QD nanocrystals were modified with 11-mercaptoundecanoic acid (MUA), L-cysteine, and lipoic acid decorated with folic acid (FA) and used as a novel TDD system for targeting doxorubicin (DOX) to folate receptors (FARs) on adenocarcinomic human alveolar basal epithelial cells (A549). NIH/3T3 cells were used as FAR-negative controls. Comprehensive physicochemical, cytotoxicity, and genotoxicity studies were performed to characterize the developed novel TDDs. Results Fourier transformation infrared spectroscopy, dynamic light scattering, and fluorescence quenching confirmed the successful attachment of FA to the QD nanocrystals and of DOX to the QD–FA nanocarriers. UV–Vis analysis helped in determining the amount of FA and DOX covalently anchored to the surface of the QD nanocrystals. Biological screening revealed that the QD–FA–DOX nanoconjugates had higher cytotoxicity in comparison to the other forms of synthesized QD samples, suggesting the cytotoxic effect of DOX liberated from the QD constructs. Contrary to the QD–MUA–FA–DOX nanoconjugates which occurred to be the most cytotoxic against A549 cells among others, no such effect was observed for NIH/3T3 cells, confirming FARs as molecular targets. In vitro scratch assay also revealed significant inhibition of A549 cell migration after treatment with QD–MUA–FA–DOX. The performed studies evidenced that at IC50 all the nanoconjugates induced significantly more DNA breaks than that observed in nontreated cells. Overall, the QD–MUA–FA–DOX nanoconjugates showed the greatest cytotoxicity and genotoxicity, while significantly inhibiting the migratory potential of A549 cells. Conclusion QD–MUA–FA–DOX nanoconjugates can thus be considered as a potential drug delivery system for the effective treatment of adenocarcinomic human alveolar basal epithelial cells.

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Cephradine drug release using electrospun chitosan nanofibers incorporated with halloysite nanoclay

Zeitschrift für Physikalische Chemie ◽

10.1515/zpch-2021-3072 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Mahwish Naz ◽

Muhammad Rizwan ◽

Sehrish Jabeen ◽

Abdul Ghaffar ◽

Atif Islam ◽

...

Keyword(s):

Drug Release ◽

Biomedical Application ◽

Drug Carrier ◽

Electrospun Nanofibers ◽

Average Diameter ◽

Sem Analysis ◽

Ftir Analysis ◽

Control Manner ◽

Release Analysis ◽

Thermal Stability Of

Abstract The chitosan/polyvinyl alcohol/halloysite nanoclay (CS/PVA/HNC) loaded with cephradine drug electrospun nanofibers (NFs) were fabricated and characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) techniques. FTIR analysis confirmed the hydrogen bonding between the polymer chain and the developed siloxane linkages. SEM analysis revealed the formation of uniform NFs having beads free and smooth surface with an average diameter in 50–200 nm range. The thermal stability of the NFs was increased by increasing the HNC concentration. The antimicrobial activity was examined against Escherichia coli and staphylococcus strains and the NFs revealed auspicious antimicrobial potential. The drug release was studied at pH 7.4 (in PBS) at 37 °C. The drug release analysis showed that 90% of the drug was released from NFs in 2 h and 40 min. Hence, the prepared NFs could be used as a potential drug carrier and release in a control manner for biomedical application.

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Formulation, Development and Characterization of Floating Beads of Diltiazem Hydrochloride

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v6i1.8883 ◽

2016 ◽

Vol 6 (1) ◽

Author(s):

Anamika Saxena Saxena ◽

Santosh Kitawat ◽

Kalpesh Gaur ◽

Virendra Singh

Keyword(s):

Drug Delivery ◽

Drug Release ◽

Drug Delivery Systems ◽

Entrapment Efficiency ◽

Repeated Administration ◽

Alginate Beads ◽

Delivery Systems ◽

Sem Analysis ◽

Formulation Development

The main goal of any drug delivery system is to achieve desired concentration of the drug in blood or tissue, which is therapeutically effective and nontoxic for a prolonged period. Various attempts have been made to develop gastroretentive delivery systems such as high density system, swelling, floating system. The recent developments of FDDS including the physiological and formulation variables affecting gastric retention, approaches to design single-unit and multiple-unit floating systems, and their classification and formulation aspects are covered in detail. Gastric emptying is a complex process and makes in vivo performance of the drug delivery systems uncertain. In order to avoid this variability, efforts have been made to increase the retention time of the drug-delivery systems for more than 12 hours. The floating or hydrodynamically controlled drug delivery systems are useful in such application. Background of the research: Diltiazem HCL (DTZ), has short biological half life of 3-4 h, requires rather high frequency of administration. Due to repeated administration there may be chances of patient incompliance and toxicity problems. Objective: The objective of study was to develop sustained release alginate beads of DTZ for reduction in dosing frequency, high bioavailability and better patient compliance. Methodology: Five formulations prepared by using different drug to polymer ratios, were evaluated for relevant parameters and compared. Alginate beads were prepared by ionotropic external gelation technique using CaCl2 as cross linking agent. Prepared beads were evaluated for % yield, entrapment efficiency, swelling index in 0.1N HCL, drug release study and SEM analysis. In order to improve %EE and drug release, LMP and sunflower oil were used as copolymers along with sodium alginate.

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Biopolymers with Medical Applications Optimized method for obtaining chitosan-based delivery systems

Revista de Chimie ◽

10.37358/rc.18.7.6411 ◽

2018 ◽

Vol 69 (7) ◽

pp. 1756-1759 ◽

Cited By ~ 1

Author(s):

Luminita Confederat ◽

Iuliana Motrescu ◽

Sandra Constantin ◽

Florentina Lupascu ◽

Lenuta Profire

Keyword(s):

Drug Delivery ◽

Drug Delivery Systems ◽

Average Diameter ◽

Delivery Systems ◽

Cross Linking ◽

Low Molecular Weight ◽

Polymeric Systems ◽

Chitosan Microparticles ◽

Degree Of Swelling

The aim of this study was to optimize the method used for obtaining microparticles based on chitosan � a biocompatible, biodegradable, and nontoxic polymer, and to characterize the developed systems. Chitosan microparticles, as drug delivery systems were obtained by inotropic gelation method using pentasodiumtripolyphosphate (TPP) as cross-linking agent. Chitosan with low molecular weight (CSLMW) in concentration which ranged between 0.5 and 5 %, was used while the concentration of cross-linking agent ranged between 1 and 5%. The characterization of the microparticles in terms of shape, uniformity and adhesion was performed in solution and dried state. The size of the microparticles and the degree of swelling were also determined. The structure and the morphology of the developed polymeric systems were analyzed by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).The average diameter of the chitosan microparticles was around 522 �m. The most stable microparticles were obtained using CSLMW 1% and TPP 2% or CSLMW 0.75%and TPP 1%. The micropaticles were spherical, uniform and without flattening. Using CSLMW in concentration of 0.5 % poorly cross-linked and crushed microparticles have been obtained at all TPP concentrations. By optimization of the method, stable chitosan-based micropaticles were obtained which will be used to develop controlled release systems for drug delivery.

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Gold–Nickel Nanowires as Nanomotors for Cancer Marker Biodetection and Chemotherapeutic Drug Delivery

ACS Applied Nano Materials ◽

10.1021/acsanm.0c03145 ◽

2021 ◽

Vol 4 (4) ◽

pp. 3377-3388

Author(s):

Gozde Yurdabak Karaca ◽

Filiz Kuralay ◽

Emre Uygun ◽

Kadir Ozaltin ◽

Sezin Eren Demirbuken ◽

...

Keyword(s):

Drug Delivery ◽

Chemotherapeutic Drug ◽

Cancer Marker ◽

Nickel Nanowires

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Fast Dissolving Electrospun Nanofibers Fabricated from Jelly Fig Polysaccharide/Pullulan for Drug Delivery Applications

Polymers ◽

10.3390/polym13020241 ◽

2021 ◽

Vol 13 (2) ◽

pp. 241

Author(s):

Thangavel Ponrasu ◽

Bei-Hsin Chen ◽

Tzung-Han Chou ◽

Jia-Jiuan Wu ◽

Yu-Shen Cheng

Keyword(s):

Drug Delivery ◽

Water Vapor Permeability ◽

Electrospun Nanofibers ◽

Average Diameter ◽

Xrd Analysis ◽

Water Soluble ◽

Vapor Permeability ◽

Uv Spectrum ◽

Sem Images ◽

Triton X

The fast-dissolving drug delivery systems (FDDDSs) are developed as nanofibers using food-grade water-soluble hydrophilic biopolymers that can disintegrate fast in the oral cavity and deliver drugs. Jelly fig polysaccharide (JFP) and pullulan were blended to prepare fast-dissolving nanofiber by electrospinning. The continuous and uniform nanofibers were produced from the solution of 1% (w/w) JFP, 12% (w/w) pullulan, and 1 wt% Triton X-305. The SEM images confirmed that the prepared nanofibers exhibited uniform morphology with an average diameter of 144 ± 19 nm. The inclusion of JFP in pullulan was confirmed by TGA and FTIR studies. XRD analysis revealed that the increased crystallinity of JFP/pullulan nanofiber was observed due to the formation of intermolecular hydrogen bonds. The tensile strength and water vapor permeability of the JFP/pullulan nanofiber membrane were also enhanced considerably compared to pullulan nanofiber. The JFP/pullulan nanofibers loaded with hydrophobic model drugs like ampicillin and dexamethasone were rapidly dissolved in water within 60 s and release the encapsulants dispersive into the surrounding. The antibacterial activity, fast disintegration properties of the JFP/pullulan nanofiber were also confirmed by the zone of inhibition and UV spectrum studies. Hence, JFP/pullulan nanofibers could be a promising carrier to encapsulate hydrophobic drugs for fast-dissolving/disintegrating delivery applications.

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β-Catenin regulates P-cadherin expression in mammary basal epithelial cells

FEBS Letters ◽

10.1016/j.febslet.2007.01.053 ◽

2007 ◽

Vol 581 (5) ◽

pp. 831-836 ◽

Cited By ~ 16

Author(s):

Marisa M. Faraldo ◽

Jérôme Teulière ◽

Marie-Ange Deugnier ◽

Walter Birchmeier ◽

Joerg Huelsken ◽

...

Keyword(s):

Epithelial Cells ◽

Basal Epithelial Cells

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Facile Electrospinning Preparation of Y3Al5O12 Nanobelts

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.448-453.3041 ◽

2013 ◽

Vol 448-453 ◽

pp. 3041-3045

Author(s):

Fei Bi ◽

Xiang Ting Dong ◽

Jin Xian Wang ◽

Gui Xia Liu ◽

Wen Sheng Yu

Keyword(s):

Sol Gel ◽

Xrd Analysis ◽

Sem Analysis ◽

Ftir Analysis ◽

X Ray ◽

Novel Structure ◽

Group I ◽

Sol Gel Process ◽

Composite Nanobelts ◽

Scanning Electron

PVP/[Y(NO3)3+Al (NO3)3] composite nanobelts were fabricated via electrospinning combined with sol-gel process and novel structure of Y3Al5O12(denoted as YAG for short) nanobelts have been obtained after calcination of the relevant composite nanobelts. The structural properties were characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). XRD analysis indicated that the composite nanobelts were amorphous, and YAG nanobelts were cubic in structure with space group Ia3d. FTIR analysis manifested that pure YAG nanobelts were formed at 900oC. SEM analysis and histograms revealed that the width of the composite nanobelts and YAG nanobelts were 3.5 μm and 2.4 μm, and the thickness were 240 nm and 112 nm, respectively, under the 95% confidence level. The formation mechanism of YAG nanobelts was discussed in detail.

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Synthesis of Catechin-Gelatin Nanofiber by Electrospinning

Materials Science Forum ◽

10.4028/www.scientific.net/msf.887.96 ◽

2017 ◽

Vol 887 ◽

pp. 96-99 ◽

Cited By ~ 1

Author(s):

Muhamad Nasir ◽

Dita Apriani

Keyword(s):

Polyethylene Oxide ◽

Chemical Structure ◽

Sem Analysis ◽

Electrospinning Process ◽

Vibration Band ◽

Ftir Analysis ◽

Tuna Fish ◽

Bioactive Component ◽

Cosmetic Industry ◽

Nanofiber Diameter

Catechin and gelatin are important natural products for food, medical, pharmaceutical and cosmetic industry. We have successfully synthesized catechin-gelatin nanofiber by electrospinning process. Catechin-gelatin nanofiber was synthesized by using gelatin from yellow fin skin tuna fish as biopolymer, polyethylene oxide (PEO) as spinnability improver polymer, acetic acid as solvent and catechin as bioactive component, respectively. Morphology and structure of bioactive catechin-gelatin nanofiber were characterized by scanning electron microscopy (SEM) and fourier transform infrared spectroscopy (FTIR), respectively. SEM analysis showed that morphology of nanofiber was very smooth without bead on nanofiber string. The average of catechin-gelatin nanofiber diameter was 389 nm. FTIR analysis results were used to confirm structure of catechin-gelatin nanofiber. Catechin-gelatin nanofiber has vibration band peak of amide A (N-H) at 3289,043 cm-1 and amide B (N-H) 3062,310 cm-1, amide I (C=O) at 1643,812 cm-1, amide II (N-H and CN) at 1538,949 cm-1, amide III (C-N) at 1237,11 cm-1 from gelatin, C-O-C from PEO at 1143,583 cm-1, and vibration band peak OH at 3200-3600 cm-1, and at C-O ether around 1300-1100 from catechin, respectively. FTIR spectra showed us that there is no change in chemical structure of gelatin and catechin in nanofiber which was produced by electrospinning process. Catechin-gelatin nanofiber can inhibit S. Aureus bacteria around 43.38%

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