Paternal Finasteride Treatment Can Influence the Testicular Transcriptome Profile of Male Offspring—Preliminary Study

(1) Background: Hormone-dependent events that occur throughout spermatogenesis during postnatal testis maturation are significant for adult male fertility. Any disturbances in the T/DHT ratio in male progeny born from females fertilized by finasteride-treated male rats (F0:Fin) can result in the impairment of testicular physiology. The goal of this work was to profile the testicular transcriptome in the male filial generation (F1:Fin) from paternal F0:Fin rats. (2) Methods: The subject material for the study were testis from immature and mature male rats born from females fertilized by finasteride-treated rats. Testicular tissues from the offspring were used in microarray analyses. (3) Results: The top 10 genes having the highest and lowest fold change values were mainly those that encoded odoriferous (Olfr: 31, 331, 365, 633, 774, 814, 890, 935, 1109, 1112, 1173, 1251, 1259, 1253, 1383) and vomeronasal (Vmn1r: 50, 103, 210, 211; Vmn2r: 3, 23, 99) receptors and RIKEN cDNA 5430402E10, also known as odorant-binding protein. (4) Conclusions: Finasteride treatment of male adult rats may cause changes in the testicular transcriptome of their male offspring, leading to a defective function of spermatozoa in response to odorant-like signals, which are recently more and more often noticed as significant players in male fertility.

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Effects of the Aqueous Extract ofEremomastax speciosa(Acanthaceae) on Sexual Behavior in Normal Male Rats

BioMed Research International ◽

10.1155/2016/9706429 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10 ◽

Cited By ~ 3

Author(s):

B. Nchegang ◽

C. Mezui ◽

F. Longo ◽

Z. E. Nkwengoua ◽

A. P. Amang ◽

...

Keyword(s):

Sexual Behavior ◽

Aqueous Extract ◽

Male Rats ◽

Normal Male ◽

Adult Rats ◽

Male Adult ◽

Positive Effects ◽

Posttreatment Period ◽

And Performance ◽

Behavior Of Rats

Objective. We studied prosexual effects ofEremomastax speciosaaqueous extract in male adult rats.Materials and Methods. 100 and 500 mg/kg of extract were administered orally (days 0, 1, 4, 7, 14, and 28 (posttreatment)). The sexual behavior of rats receiving a single dose (500 mg/kg) was also evaluated after pretreatment with Lω-NAME (10 mg/kg), haloperidol (1 mg/kg), or atropine (5 mg/kg). Controls received distilled water or testosterone enanthate (20 mg/kg/day/3 days(s.c.)before the test).Results. The extract (days 1–14) had no significant effect on mount, intromission, and ejaculation frequencies but on day 28 (14 days after treatment), it increased frequency of mounts and intromissions at 500 mg/kg. Mount, intromission, and ejaculation latencies reduced and postejaculatory intervals decreased but the effect did not persist 2 weeks after treatment. Extract prosex effects were greatly reduced by atropine and completely abolished by haloperidol, while Lω-NAME increased mount latency and potentiated extract effect on intromission and ejaculation latencies.Conclusion. In summary,E. speciosaextract can have positive effects on male sexual motivation and performance when administered for two weeks at the dose of 500 mg/kg. The effects (dopaminergic and/or cholinergic dependent) tend to appear during the posttreatment period.

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Reproductive toxicity and tissue concentrations of 3,3',4,4'-tetrachlorobiphenyl (PCB 77) in male adult rats

Human & Experimental Toxicology ◽

10.1177/096032719801700305 ◽

1998 ◽

Vol 17 (3) ◽

pp. 151-156 ◽

Cited By ~ 1

Author(s):

A S Faqi ◽

P R Dalsenter ◽

W Mathar ◽

B Heinrich-Hirsch ◽

I Chahoud

Keyword(s):

Adult Male ◽

Weight Control ◽

Reproductive Toxicity ◽

Serum Testosterone ◽

Male Rats ◽

Testis Weight ◽

Adult Rats ◽

Male Adult ◽

Reproductive Effects ◽

Investigation Period

1 The aim of this study was to ascertain the reproductive effects of PCB 77 on adult male rats and to determine its concentration in the liver and testis. Adult male rats (n=15/group) were treated subcutaneously with a singledoseof18 mg/kgbw(PC18)orwith60 mg/kg bw (PC60). The substance was dissolved in a 10 ml volume of peanut oil/kg. Control rats received the same volume of the vehicle. The reproductive effects as well as the concentration of PCB 77 in the liver and testis were investigated 1, 4 and 8 weeks after treatment. 2 In both groups, the daily sperm production (DSP; 6106) remained permanently reduced in the PC18 as well as in the PC60 groups throughout the entire investigation period (DSP week 8: control: 31+7; PC18: 22+5; PC60: 20+7). The sperm number (6106) per cauda epididymis was affected only at the 1st and 4th week after treatment (control week 1: 211+67; PC18 week 1: 135+62; PC60 week 1: 142+49). Moreover, a significant increase in the percentage of abnormal sperm was observed 4 weeks following treatment in the PC18 and PC60 groups and 8 weeks after treatment in the PC60 group. Abnormal tails were the most frequent changes observed. 3 The relative testicular and prostata weights (g) were slightly increased in the PC60 group at the 1st and 4th week following treatment (testis weight: control/I: 0.46+0.02; PC60/I: 0.51+0.03). 4 The serum testosterone concentrations and effects on testis morphology were not reported. 5 The maximum concentration of PCB 77 was detected in the liver and testis 1 week after treatment. The concentration declined 4 weeks after treatment in both organs, but still a significant amount of PCB 77 was detectable in the liver as well as in the testis 8 weeks after treatment. 6 The results demonstrate that PCB 77 affects sperm variables when applied to adult rats and that the elimination of PCB 77 in the testis parallels that of the liver.

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Microheterogeneity of pituitary follicle-stimulating hormone in male rats: differential effects of the chronic androgen deprivation induced by castration or androgen blockade

Journal of Molecular Endocrinology ◽

10.1677/jme.0.0090175 ◽

1992 ◽

Vol 9 (2) ◽

pp. 175-182 ◽

Cited By ~ 13

Author(s):

M. Simoni ◽

G. F. Weinbauer ◽

R. K. Chandolia ◽

E. Nieschlag

Keyword(s):

Male Rats ◽

Male Rat ◽

Molecular Heterogeneity ◽

Significant Shift ◽

Relative Distribution ◽

Qualitative Properties ◽

Adult Rats ◽

Male Adult ◽

Androgen Blockade

ABSTRACT Testicular androgens are known to influence not only the secretion but also the bioactivity and molecular composition of pituitary FSH. In the present study, we investigated the effects of chronic androgen blockade and castration on the molecular heterogeneity of the gonadotrophin. Groups of male adult rats (five animals per group) received one of the following treatments: vehicle, the non-steroidal anti-androgens casodex (20 mg/kg per day) or flutamide (20 mg/kg per day), or castration. After 8 weeks, the animals were killed and individual pituitary homogenates fractionated by isoelectric focusing (IEF) on sucrose density gradients in the pH range 2·5–8. FSH was measured by radioimmunoassay (RIA) in the individual fractions and by in-vitro bioassay (Sertoli cell aromatase bioassay) in pools of fractions which were combined according to pH intervals of 0·5 units. Bioactive and immunoreactive FSH were also measured in sera and unfractionated pituitary extracts. Testosterone and inhibin were assayed in sera by RIA. A significant increase in serum immunoreactive and bioactive FSH was demonstrated in flutamide-treated and castrated animals, whereas the pituitary content of bioactive FSH remained unchanged in the four groups. Serum testosterone and inhibin were undetectable in castrated animals and significantly increased in those treated with flutamide. By RIA, the IEF profiles of the flutamide-treated and castrated rats showed a significant reduction of the FSH isoforms with 3·5<pI<4, with a significant increase in the isoforms with pI>4 only in the castrated group. By bioassay, there was a significant decrease in the isoforms with 3·5<pI<4 in both casodex- and flutamide-treated animals, with no significant differences between the two groups. Castration caused a further significant shift in the relative distribution of FSH isoforms towards the less acidic components, with a significant increase in the isoforms with 5<pI<5·5 not attained by androgen blockade alone. These results suggest that the effects of long-lasting castration on pituitary FSH heterogeneity cannot be entirely reproduced by the androgen blockade. Since inhibin, eliminated by castration but not by androgen blockade, is a major regulator of FSH in the male rat, we speculate that it might not only influence FSH secretion but also modulate its qualitative properties.

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Fasting Enhances Pyroglutamyl Peptidase II Activity in Tanycytes of the Mediobasal Hypothalamus of Male Adult Rats

Endocrinology ◽

10.1210/en.2014-1885 ◽

2015 ◽

Vol 156 (7) ◽

pp. 2713-2723 ◽

Cited By ~ 18

Author(s):

Iván Lazcano ◽

Agustina Cabral ◽

Rosa María Uribe ◽

Lorraine Jaimes-Hoy ◽

Mario Perello ◽

...

Keyword(s):

Male Rats ◽

Mrna Levels ◽

Mediobasal Hypothalamus ◽

Adult Rats ◽

Male Adult ◽

In Situ Hybridization Histochemistry ◽

Corticosterone Treatment ◽

Hpt Axis ◽

Partial Reversion

Fasting down-regulates the hypothalamus-pituitary-thyroid (HPT) axis activity through a reduction of TRH synthesis in neurons of the parvocellular paraventricular nucleus of the hypothalamus (PVN). These TRH neurons project to the median eminence (ME), where TRH terminals are close to the cytoplasmic extensions of β2 tanycytes. Tanycytes express pyroglutamyl peptidase II (PPII), the TRH-degrading ectoenzyme that controls the amount of TRH that reaches the anterior pituitary. We tested the hypothesis that regulation of ME PPII activity is another mechanism by which fasting affects the activity of the HPT axis. Semiquantitative in situ hybridization histochemistry data indicated that PPII and deiodinase 2 mRNA levels increased in tanycytes after 48 hours of fasting. This increase was transitory, followed by an increase of PPII activity in the ME, and a partial reversion of the reduction in PVN pro-TRH mRNA levels and the number of TRH neurons detected by immunohistochemistry. In fed animals, adrenalectomy and corticosterone treatment did not change ME PPII activity 72 hours later. Methimazole-induced hypothyroidism produced a profound drop in tanycytes PPII mRNA levels, which was reverted by 3 days of treatment with T4. The activity of thyroliberinase, the serum isoform of PPII, was increased at most fasting time points studied. We conclude that delayed increases in both the ME PPII as well as the thyroliberinase activities in fasted male rats may facilitate the maintenance of the deep down-regulation of the HPT axis function, despite a partial reactivation of TRH expression in the PVN.

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Effect of chronic administration of an aromatase inhibitor to adult male rats on pituitary and testicular function and fertility

Journal of Endocrinology ◽

10.1677/joe.0.1640225 ◽

2000 ◽

Vol 164 (2) ◽

pp. 225-238 ◽

Cited By ~ 78

Author(s):

KJ Turner ◽

M Morley ◽

N Atanassova ◽

ID Swanston ◽

RM Sharpe

Keyword(s):

Drinking Water ◽

Aromatase Inhibitor ◽

Germ Cells ◽

Sertoli Cells ◽

Female Rats ◽

Male Rats ◽

Histological Evaluation ◽

Testis Weight ◽

Adult Rats ◽

Male Adult

The aim of the present study was to evaluate the effects of the administration of a potent non-steroidal aromatase inhibitor, anastrozole, on male reproductive function in adult rats. As anastrozole was to be administered via the drinking water, a preliminary study was undertaken in female rats and showed that this route of administration was effective in causing a major decrease in uterine weight (P<0.02). In an initial study in male adult rats, anastrozole (100 mg/l or 400 mg/l) was administered via the drinking water for a period of 9 weeks. Treatment with either dose resulted in a significant increase ( approximately 10%) in testis weight and increase in plasma FSH concentrations (P<0.01) throughout the 9 weeks. Mating was altered in both groups of anastrozole-treated rats, as they failed to produce copulatory plugs. Histological evaluation of the testes from anastrozole-treated rats revealed that spermatogenesis was grossly normal. In a more detailed study, adult rats were treated with 200 mg/l anastrozole via the drinking water for periods ranging from 2 weeks to 1 year. Plasma FSH and testosterone concentrations were increased significantly (P<0.001) during the first 19 weeks of treatment. However, LH concentrations were increased only at 19 weeks (P<0.001) in anastrozole-treated rats, and this coincided with a further increase in circulating and intratesticular testosterone concentrations (P<0.05). No consistent change in inhibin-B concentrations was observed during the study. Suppression of plasma oestradiol concentrations could not be demonstrated in anastrozole-treated animals, but oestradiol concentrations in testicular interstitial fluid were reduced by 18% (P<0.01). Mating was again inhibited by anastrozole treatment, but could be restored by s.c. injection of oestrogen, enabling demonstration that rats treated for 10 weeks or 9 months were still fertile. Testis weight was increased by 19% and 6% after treatment for 19 weeks and 1 year, respectively. Body weight was significantly decreased (P<0.01) by 19 weeks of anastrozole treatment; after 1 year the animals appeared to have less fat as indicated by a 27% decrease in the weight of the gonadal fat pad. The majority of anastrozole-treated animals had testes with normal spermatogenesis but, occasionally, seminiferous tubules showed abnormal loss of germ cells or contained only Sertoli cells. Ten percent of anastrozole-treated animals had testes that appeared to contain only Sertoli cells, and one rat had 'giant' testes in which the tubule lumens were severely dilated. Morphometric analysis of the normal testes at 19 weeks showed no difference in the number of Sertoli cells or germ cells, or the percentage volumes of the seminiferous epithelium, tubule lumens and interstitium between control and anastrozole-treated rats. On the basis of the present findings, oestrogen appears to be involved in the regulation of FSH secretion and testosterone production, and is also essential for normal mating behaviour in male rats. Furthermore, these data suggest that the brain and the hypothalamo-pituitary axis are considerably more susceptible than is the testis to the effects of an aromatase inhibitor. Anastrozole treatment has resulted in a model of brain oestrogen insufficiency.

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Investigation of the Zinc Oxide Nanoparticles Effect on Thyroid and Testosterone Hormones in Male Rats

Cihan University-Erbil Scientific Journal ◽

10.24086/cuesj.v4n1y2020.pp26-31 ◽

2020 ◽

Vol 4 (1) ◽

pp. 26-31

Author(s):

Noori M. Luaibi ◽

Noor A. Zayed

Keyword(s):

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Thyroid Stimulating Hormone ◽

Male Rats ◽

Oxide Nanoparticles ◽

Sprague Dawley ◽

Zno Nps ◽

Adult Rats ◽

Male Adult ◽

Intraperitoneal Dose

Exposure to zinc oxide nanoparticles (ZnO NPs) has been increasing steadily, causing more attention being paid to their potential toxicity, including cytotoxicity and genotoxicity. Hence, this study aimed to investigate the effect of ZnO NPs on thyroid hormone triiodothyronine (T3), thyroxine (T4), and thyroid-stimulating hormone (TSH) as well as testosterone hormone in male adult rats. A total of 54 Sprague-Dawley albino adult male rats were divided into nine groups each of six rats, daily treated intraperitoneal with ZnO NPs two different doses (30 and 60) mg/kg in three different periods of time (7, 14, and 28) days, as following: Control groups (Groups 1, 2, and 3): Respectively received intraperitoneal injection with distilled water for 7, 14, and 28 days, experimental groups (Groups 4, 5, and 6): They were rats, respectively, received intraperitoneal dose (60 mg/kg) of ZnO NPs for (7, 14, and 28) days, and group (7, 8, and 9) experimental groups were rats, respectively, received intraperitoneal dose (30 mg/kg) of ZnO NPs for (7, 14, and 28) days. Data showed high significant decrease (P < 0.01) in level of T3, T4, TSH, and level of testosterone also decrease at high and low dose for 7, 14, and 28 days.

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Specimen Collection Effect in Scanning Electron Microscopy Images

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100095492 ◽

1972 ◽

Vol 30 ◽

pp. 448-449

Author(s):

J. Temple Black ◽

Jose Guerrero

Keyword(s):

Surface Morphology ◽

Secondary Electron Emission ◽

Secondary Electrons ◽

Charge Effects ◽

Material Density ◽

Specimen Collection ◽

The Subject ◽

Curved Paths ◽

Subject Material ◽

Microscopy Images

In the SEM, contrast in the image is the result of variations in the volume secondary electron emission and backscatter emission which reaches the detector and serves to intensity modulate the signal for the CRT's. This emission is a function of the accelerating potential, material density, chemistry, crystallography, local charge effects, surface morphology and especially the angle of the incident electron beam with the particular surface site. Aside from the influence of object inclination, the surface morphology is the most important feature In producing contrast. “Specimen collection“ is the name given the shielding of the collector by adjacent parts of the specimen, producing much image contrast. This type of contrast can occur for both secondary and backscatter electrons even though the secondary electrons take curved paths to the detector-collector.Figure 1 demonstrates, in a unique and striking fashion, the specimen collection effect. The subject material here is Armco Iron, 99.85% purity, which was spark machined.

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INDUCTION OF GOITRE BY PTU OR KClO4 IN MALE AND FEMALE RATS. EFFECT OF GONADECTOMY

Acta Endocrinologica ◽

10.1530/acta.0.0740088 ◽

1973 ◽

Vol 74 (1) ◽

pp. 88-104 ◽

Cited By ~ 2

Author(s):

T. Jolín ◽

M. J. Tarin ◽

M. D. Garcia

Keyword(s):

Iodine Content ◽

High Plasma ◽

Disc Electrophoresis ◽

Female Rats ◽

Male Rats ◽

Adult Rats ◽

Male And Female ◽

Glucose Levels ◽

Male And Female Rats ◽

Tsh Levels

ABSTRACT Male and female rats of varying ages were placad on a low iodine diet (LID) plus KClO4 or 6-propyl-2-thiouracil (PTU) or on the same diet supplemented with I (control rats). Goitrogenesis was also induced with LID plus PTU in gonadectomized animals of both sexes. The weight of the control and goitrogen treated animals, and the weight and iodine content of their thyroids were determined, as well as the plasma PBI, TSH, insulin and glucose levels. The pituitary GH-like protein content was assessed by disc electrophoresis on polyacrylamide gels. If goitrogenesis was induced in young rats of both sexes starting with rats of the same age, body weight (B.W.) and pituitary growth hormone (GH) content, it was found that both the males and females developed goitres of the same size. On the contrary, when goitrogenesis was induced in adult animals, it was found that male rats, that had larger B.W. and pituitary GH content than age-paired females, developed larger goitres. However, both male and female rats were in a hypothyroid condition of comparable degree as judged by the thyroidal iodine content and the plasma PBI and TSH levels. When all the data on the PTU or KClO4-treated male and female rats of varying age and B.W. were considered together, it was observed that the weights of the thyroids increased proportionally to B.W. However, a difference in the slope of the regression of the thyroid weight over B.W. was found between male and female rats, due to the fact that adult male rats develop larger goitres than female animals. In addition, in the male rats treated with PTU, gonadectomy decreased the B.W., pituitary content of GH-like protein and, concomitantly, the size of the goitre decreased; an opposite effect was induced by ovariectomy on the female animals. However, when goitrogenesis was induced in weight-paired adult rats of both sexes, the male animals still developed larger goitres than the females. Among all the parameters studied here, the only ones which appeared to bear a consistent relationship with the size of the goitres in rats of different sexes, treated with a given goitrogen, were the rate of body growth and the amount of a pituitary GH-like protein found before the onset of the goitrogen treatment. Moreover, though the pituitary content of the GH-like protein decreased as a consequence of goitrogen treatment, it was still somewhat higher in male that in female animals. The present results suggest that GH may somehow be involved in the mechanism by which male and female rats on goitrogens develop goitres of different sizes, despite equally high plasma TSH levels.

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South Sea Tales

10.1093/owc/9780199536085.001.0001 ◽

2008 ◽

Author(s):

Robert Louis Stevenson

Keyword(s):

Nineteenth Century ◽

Cross Cultural ◽

Robert Louis Stevenson ◽

Global Culture ◽

Cultural Encounters ◽

Post Colonial ◽

The Pacific ◽

South Sea ◽

The Subject ◽

Subject Material

The literary world was shocked when in 1889, at the height of his career, Robert Louis Stevenson announced his intention to settle permanently on the Pacific island of Samoa. His readers were equally shocked when he began to use the subject material offered by his new environment, not to promote a romance of empire, but to produce some of the most ironic and critical treatments of imperialism in nineteenth-century fiction. In these stories, as in his work generally, Stevenson shows himself to be a virtuoso of narrative styles: his Pacific fiction includes the domestic realism of ‘The Beach at Falesé, the folktale plots of ‘The Bottle Imp’ and ‘The Isle of Voices’, and the modernist blending of naturalism and symbolism in The Ebb-Tide. But beyond their generic diversity the stories are linked by their concern with representing the multiracial society of which their author had become a member. In this collection - the first to bring together all his shorter Pacific fiction in one volume - Stevenson emerges as a witness both to the cross- cultural encounters of nineteenth-century imperialism and to the creation of the global culture which characterizes the post-colonial world.

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