scholarly journals Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot study

Cosmetics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 82
Author(s):  
Elizabeta Lohova ◽  
Mara Pilmane ◽  
Mara Rone-Kupfere ◽  
Janis Kisis
Keyword(s):  
Connective Tissue ◽  
Control Group ◽  
Strong Positive Correlation ◽  
Facial Skin ◽  
Significant Difference ◽  
Tissue Factors ◽  
Affected Tissue ◽  
Anti Inflammatory ◽  
Inflammatory Tissue

Rosacea is a chronic inflammatory skin disease mainly affecting the facial skin. Our aim was to determine the appearance of pro- and anti- inflammatory cytokines in rosacea-affected facial tissue. Materials and Methods: Rosacea tissue were obtained from eight patients (aged 35 to 50 years). The control group (CG) included four facial skin samples (49 to 70 years). Routine staining and immunohistochemistry for IL-1, IL-10, LL-37, HBD-2, and HBD-4 proceeded. Results: Inflammation was observed in all the rosacea samples. A statistically significant difference was seen between epithelial HBD-2 positive cells in comparison to the control. There was a strong positive correlation between HBD-4 in the epithelium and HBD-4 in the connective tissue, IL-10 in the epithelium and IL-1 in the connective tissue, and IL-1 in the epithelium and IL-10 in the connective tissue. Conclusion: Increased levels of IL-10 and decreased levels of IL-1 show the balance between anti- and pro-inflammatory tissue responses. A significant amount of HBD-2 in the epithelium proves its important role in the local immune response of rosacea-affected tissue. The last effect seems to be intensified by the elevated level of LL-37 in the epithelium.

scholarly journals Characterization of Cytokines and Proliferation Marker Ki67 in Cleft Affected Lip Tissue

Medicina ◽  
2019 ◽  
Vol 55 (9) ◽  
pp. 518 ◽  
Author(s):  
Pilmane ◽  
Sidhoma ◽  
Akota ◽  
Kazoka
Keyword(s):  
Immune Response ◽  
Connective Tissue ◽  
Cleft Lip ◽  
Cellular Proliferation ◽  
Tissue Response ◽  
Compensatory Mechanism ◽  
Strong Positive Correlation ◽  
Proliferation Marker ◽  
Significant Difference ◽  
Anti Inflammatory

Background and objectives: Cleft lip palate takes the second place among all anomalies. The complex appearance of cytokines and proliferation markers has still not been clarified despite their possible crucial role in cleft tissue. Therefore, the aim of work was the detection of appearance of pro- and anti-inflammatory cytokines and proliferation marker Ki67, and their inter-correlations in cleft affected lip (CAL). Materials and Methods: The lip material was obtained from 16 children aged before primary dentition during plastic surgery. Control was obtained from 7 non-CAL oral tissue. Tissues were stained for IL-1, IL-4, IL-6, IL-8, IL-10 and Ki67 immunohistochemically. Non-parametric statistic, Mann–Whitney and Spearman’s coefficient were used. Results: All cytokines positive cells were observed more into the epithelium. Statistically significant difference was seen between epithelial IL-1, IL-10, IL-8 and Ki67 positive cells and IL-10-, IL-4-containing connective tissue cells in comparison to the control. Strong positive correlation was detected in CAL epithelium between IL-10 and IL-8, IL-10 and IL-4, IL-10 and IL-1, IL-1 and IL-8, IL-1 and IL-4, IL-4 and IL-8, IL-8 and Ki67, IL-10 and Ki67, but moderate—in connective tissue between IL-1 and IL-10, IL-1 and IL-4. Conclusion: The CAL epithelium is the main source for the interleukins. Rich similar expression of IL-1 and IL-10 suggests the balance between pro-and anti-inflammatory tissue response on basis of dysregulated tissue homeostasis (increase of IL-8). The correlations between the different ILs -1, -4, -8, -10 in CAL epithelium seem to indicate the self-protection compensatory mechanism for intensification of local inflammatory-immune response without involvement of IL-6. The correlations between Ki67 and cytokines indicate the involvement of IL-8 and IL-10 in stimulation of cellular proliferation. IL-4 and IL-10 expression from CAL connective tissue simultaneously to IL-1, IL-4 and IL-10 inter-correlations there suggests the intensification of local immune response regulated probably by main pro-inflammatory cytokine—IL-1.

Comparison of hematological parameters and perinatal outcomes between COVID-19 pregnancies and healthy pregnancy cohort

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Esin Merve Erol Koç ◽  
Rahime Bedir Fındık ◽  
Hatice Akkaya ◽  
Işılay Karadağ ◽  
Eda Özden Tokalıoğlu ◽  
...  
Keyword(s):  
Complete Blood Count ◽  
Perinatal Outcomes ◽  
Control Group ◽  
Strong Positive Correlation ◽  
Monocyte Count ◽  
Distribution Width ◽  
Neutrophil Lymphocyte Ratio ◽  
Lymphocyte Ratio ◽  
Significant Difference ◽  
Adverse Perinatal Outcomes

AbstractObjectivesTo evaluate the relationship between Coronavirus Disease 2019 (COVID-19) in pregnancy and adverse perinatal outcomes. The secondary aim is to analyze the diagnostic value of hematologic parameters in COVID-19 complicated pregnancies.MethodsThe current study is conducted in a high volume tertiary obstetrics center burdened by COVID-19 pandemics, in Turkey. In this cohort study, perinatal outcomes and complete blood count indices performed at the time of admission of 39 pregnancies (Study group) complicated by COVID-19 were compared with 69 uncomplicated pregnancies (Control group).ResultsThere was no significant difference between the obstetric and neonatal outcomes of pregnancies with COVID-19 compared to data of healthy pregnancies, except the increased C-section rate (p=0.026). Monocyte count, red cell distribution width (RDW), neutrophil/lymphocyte ratio (NLR), and monocyte/lymphocyte ratio (MLR) were significantly increased (p<0.0001, p=0.009, p=0.043, p<0.0001, respectively) whereas the MPV and plateletcrit were significantly decreased (p=0.001, p=0.008) in pregnants with COVID-19. ROC analysis revealed that the optimal cut-off value for MLR was 0.354 which indicated 96.7% specificity and 59.5% sensitivity in diagnosis of pregnant women with COVID-19. A strong positive correlation was found between the MLR and the presence of cough symptom (r=41.4, p=<0.0001).ConclusionsThe study revealed that, pregnancies complicated by COVID-19 is not related with adverse perinatal outcomes. MLR may serve as a supportive diagnostic parameter together with the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) in assessment of COVID-19 in pregnant cohort.

scholarly journals Evaluation of effect of local exogenous application of Myrrh oil on healing of wound incisions of facial skin (Histochemical, Histological and Histomorphometrical study in rabbits)

2019 ◽  
Vol 31 (4) ◽  
Author(s):  
Nawar Bahjet Kamil ◽  
Nada M. Al-Ghaban
Keyword(s):  
Healing Process ◽  
Inflammatory Cells ◽  
Control Group ◽  
Facial Skin ◽  
Histological Findings ◽  
Control Groups ◽  
Mean Values ◽  
Significant Difference ◽  
The Right ◽  
New Zealand Rabbits

Aim of the study: Is to evaluate the effect of myrrh oil local application on the healing process of skin wounds histologically , histomorphometrically and , histochemically. Materials and methods:Twenty male white New Zealand rabbits were used in this study. An incisional wounds with full thickness depth and of 2 cm length were done on both sides of the cheek skin of each rabbit. The left sided incisions (the control group) were irrigated with distilled water (10µL). The right sided incisions (the experimental groups) were treated with myrrh oil (10µL). Each group was subdivided into 4 subgroups according to the healing interval into 1,3,7 and 14 days(5 rabbits for each group). Results: Histological findings of our current study showed a highly significant difference between the experimental and the control groups in count of the inflammatory cells which showed that mean values increased with time for the control and the experimental groups. The histomorphometrical findings had shown that the thickness of the epithelium was nearly completed at about 7 days for the experimental groups and at about 14 days for the control. The blood vessels count was recorded to have a highly significant difference between the groups at days 1 and 3 only. The histochemical findings had shown that the collagen fibers remodeling had recorded a highly significant difference between the control and the experimental groups at days 7 and 14. Conclusion: The current study had revealed that myrrh oil accelerates wounds healing in rabbits. Key word:Wound healing , Myrrh oil, Masson’s Trichrome staining.

scholarly journals Anti-Inflammatory Effect of Musa acuminata Stem

2020 ◽  
Vol 14 (02) ◽  
pp. 294-298
Author(s):  
Maharani Laillyza Apriasari ◽  
Selviana Rizky Pramitha ◽  
Dewi Puspitasari ◽  
Diah Savitri Ernawati
Keyword(s):  
Treatment Group ◽  
Musa Acuminata ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Tnf Α ◽  
Significant Difference ◽  
Anti Inflammatory ◽  
Mucosal Wound ◽  
Inflammatory Effect

Abstract Objective This study was designed to assess the anti-inflammatory effect of Musa acuminata through the expression of tumor necrosis factor-α (TNF-α) and nuclear factor kappa β (NF-κB) after 3 days of application of Musa acuminata stem extract (MASE) gel on oral mucosal wound. Materials and Methods An experimental study with post-test only control group design was conducted. Twenty male Rattus norvegicus (Wistar) were injured on their left buccal mucosa and treated three times a day with MASE gel of varying concentrations: 0% (as control), MASE 25%, MASE 37.5%, and MASE 50%. On day 3, a biopsy was performed on each mucosal wound for later immunohistochemical analysis for the expressions of TNF-α and NF-κB. Results The highest expression of TNF-α was observed in the control group (13.20 ± 1.79), while the lowest was in the treatment group using 50% MASE (6.40 ± 1.14). Meanwhile the comparison between treatment groups did not highlight any significant difference (p > 0.05). The highest expression of NF-κB was observed in the control group (13.20 ± 1.30), whereas the lowest was in the treatment group using MASE 50% (6.40 ± 1.14). NF-κB was significantly lower in the treatment group using MASE 50% when compared with other treatment groups (p < 0.05). Conclusion Application of MASE on mucosal wound reduces the expression of TNF-α and NF-κB at all concentrations. The anti-inflammatory effect of MASE 50% was the strongest one.

Postoperative complications following the Fontan procedure: the role of aprotinin

Perfusion ◽  
2011 ◽  
Vol 26 (6) ◽  
pp. 529-535 ◽  
Author(s):  
D Klugman ◽  
MT Donofrio ◽  
D Zurakowski ◽  
RA Jonas
Keyword(s):  
Postoperative Complications ◽  
Chest Tube ◽  
Fontan Procedure ◽  
Fontan Operation ◽  
Multivariate Logistic Regression Analysis ◽  
Chest Tube Drainage ◽  
Control Group ◽  
Tube Drainage ◽  
Significant Difference ◽  
Anti Inflammatory

Objective: To determine how the anti-inflammatory properties of aprotinin impact on postoperative complications in children undergoing the Fontan procedure. Methods: We included all patients between 14 months and 18 years (n=56) undergoing a Fontan operation at our institution between January 2005 and June 2009. The study group (n=29) included patients from January 2005 through December 2007 all of whom received aprotinin. The control group (n=27) included all patients from January 2008 through June 2009 who did not receive aprotinin. We reviewed all medical records and collected preoperative, intraoperative and postoperative data. Duration and volume of chest tube drainage were the primary outcome measures. Results: Of the 20% of patients who had postoperative arrhythmias, multivariate logistic regression analysis demonstrated only aprotinin was associated with significantly decreased postoperative arrhythmias ( P=0.01). Renal function and fenestration or Fontan thrombosis did not differ significantly; there was no statistically significant difference in volume or duration of chest tube drainage. Median duration of chest tube drainage was 7 days in the aprotinin group and 8 days for patients who did not receive aprotinin ( P=0.36). Conclusion: The anti-inflammatory properties of aprotinin may be protective against postoperative arrhythmias. Aprotinin does not confer increased risks of prolonged chest tube drainage, renal dysfunction or thrombosis in patients undergoing the Fontan procedure.

scholarly journals PENGARUH PEMBERIAN EKSTRAK METHANOL DAUN Acanthus ilicifolius TOPIKAL TERHADAP JUMLAH MAKROFAG PADA PENYEMBUHAN MODEL ORAL CANDIDIASIS DENGAN KONDISI IMUNOSUPRESI

DENTA ◽  
2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Dwi Andriani ◽  
Diana Soesilo ◽  
Ghora Setyawan
Keyword(s):  
Candida Albicans ◽  
Methanol Extract ◽  
Oral Candidiasis ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Post Test ◽  
Anti Inflammatory ◽  
Corticosteroid Drugs ◽  
Anti Fungal

<p><strong>Background:</strong> <em>Corticosteroid has a strong anti-inflammatory effect, unfortunately long-term usege of corticosteroid drugs can suppressed immune system. This condition makes Candida albicans as an opportunistic phatogen caused Oral Candidiasis. Macrophages have an important role in the first defense against infection. Methanol extract of A.ilicifolius leaf  has anti-fungal, anti-inflammatory compounds to fight the growth of Candida albicans. <strong>Purpose:</strong> To determine the effect of methanol extract of A.ilicifolius leaf theraphy to the amount of macrophages in the healing pricess of oral candidiasis models with immunosuppressed conditions. <strong>Method:</strong> </em><em>This study was true experimental with post test only control group design. Thirty-six strain wistar, 3 months old, and 250 grams of weight which were given dexamethason 0.5 mg and tetracycline 0.5mg for 7days, after Candida albican(ATCC10231) was induced for 2 weeks (3 times/week). Rats were divided into 6 groups: untreated(K-), nystatin(K+), A.ilicifolius 8%(P1), A.ilicifolius 12%(P2), A.ilicifolius 16%(P3), A.ilicifolius 20%(P4 ) all groups were treated for 2 weeks. After being treated, the rats tongue biopsied to examine the macrophages with Haematoxylin-eosin staining and observed with microscope (400x magnification). Data analyzed statistically by One-way ANOVA. <strong>Results:</strong> the amount of macrophages at K+(47.60±4.16), P4(40.20±3.56), P3(36.20±1.92), P2(17.00±2.45) were greater than K-(15.60±4.56), P1(14.60±2.70). there are no significant difference between group K- with P1 and P2, P3 with P4. There are significant difference between group K- with K+, K- with P3 and P4. K+ with P1, P2, P3 and P4. Group P1 with P3 and P4. Group P2 with P3 and P4. </em></p><strong><em>Conclusion:</em></strong><em> A.ilicifolius extract can increase the amount of macrophages in oral candidiasis immunosuppression models</em>

Identification and characterization of antibodies against human Hsp60 at pregnancy

2016 ◽  
pp. 75-78
Author(s):  
M. Makarenko ◽  
◽  
D. Govsieiev ◽  
R. Vorona ◽  
A. Tsisarenko ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Main Group ◽  
Control Group ◽  
Significant Difference ◽  
Positive Serum ◽  
Healthy Control ◽  
Shock Proteins ◽  
Identification And Characterization ◽  
Human Hsp60

Heat shock proteins (HSPs) or molecular chaperones were originally described as members of the cell response to different types of stress (infection, the influence of heavy metals, ischemia, hypoxia, amino acid starvation, psycho-emotional stress, hormonal stress, etc.). Hsp60 is one of the first proteins that are synthesized during embryogenesis. Hsp60 is necessary for the development of the embryo. The objective: was the identification and characterization of anti-Hsp60 autoantibodies in pregnant women to assess the prospects for a possible use as additional capabilities for the prediction of pregnancy progress. Patients and methods. Was a group of 170 pregnant women. 20 pregnant women were clinically healthy (control group) and 150 pregnant women had complicated anamnesis (main group). The level of IgG anti-Hsp60 antibodies in the serum was determined using immunoassay (ІFA). Immunoreactivity was determined by Western blot (immunoblotting). Results. Using the ІFA method, anti-Hsp60 positive serum was detected in 7.7% of pregnant women from the control group and in 14.0% of pregnant with complicated anamnesis. A statistically significant difference between the level of anti-Hsp60 autoantibody (on average) in pregnant women of the main group and the control group was not identified (0.177±0.118 vs. 0.134±0.097 units of optical density, p>0.05). Also, it was determined the frequency of detection of pathologies in pregnant women which have an anti-Hsp60 positive serum, compared with pregnant women which have an anti-Hsp60 negative serum. Conclusions. Our results suggest that in relation to the anti-Hsp60 antibody studies as an additional test, in particular, the forecast of progress of pregnancy, joint use ІFA and Western blotting is more appropriate. Key words: pregnancy, Hsp60, anti-Hsp60 autoantibodies.

Pseudonormokalemia in Patients with Thrombocytosis.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 2420-2420
Author(s):  
Raimonda Goldman ◽  
Amir Steinberg ◽  
Jennifer Carreiro ◽  
Georgia Panagopoulos ◽  
Marvin Cooper ◽  
...  
Keyword(s):  
Platelet Count ◽  
Serum Potassium ◽  
Conflicts Of Interest ◽  
Elevated Serum ◽  
Plasma Potassium ◽  
Control Group ◽  
Computer Search ◽  
Strong Positive Correlation ◽  
Significant Difference ◽  
The Difference

Abstract Abstract 2420 Poster Board II-397 Introduction: Pseudohyperkalemia represents an artificial elevation in serum potassium concentration. It is well described that patients with thrombocytosis may have elevated serum but normal plasma potassium. The difference between serum and plasma potassium is felt to be due to potassium release from platelets during clotting. We propose that a similar mechanism will lead to “pseudonormokalemia,” where serum potassium appears to be in the normal range (3.5-5.0 MEq/L) despite below-normal levels in the plasma(<3.5 MEq/L). Method: The interim analysis of this study was sent for consideration to ASH Annual Meeting on November 2006 (108: 3964) We now present the final analysis by comparing 69 thrombocytosis patients (platelets>500,000/uL) to 68 control patients (platelets<500,000/uL). Patients were identified from a list of lab results generated by a computer search and serum and plasma potassium and CBC were then drawn simultaneously. Results: There was no statistically significant difference in sex distribution or age between the two groups. The average platelet count was 667,960/uL in the thrombocytosis group (SD 151,1 uL) and 286,790/uL (SD 103,9 uL) in the control group (p value <0.001). In both groups, serum potassium was higher than plasma potassium. The analysis comparing serum potassium levels between the two groups showed that serum potassium was significantly higher in the thrombocytosis group than in the control. In fact, we found a strong positive correlation (spearman's rho=0.61, p<.001) between platelet count and the difference in values in serum potassium minus plasma potassium. As the platelet count increases, so does the difference between the serum and plasma potassium. The thrombocytosis group was noted to have a difference between serum and plasma potassium of .51 MEq/L (SD .30 MEq/L) while the control group had a difference of .21 MEq/L (SD .20 MEq/L) Conclusion: Patients with thrombocytosis and normal serum potassium may actually be hypokalemic as this study demonstrates. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Serum and Urinary Interleukin-6 in Assessment of Renal Activity in Egyptian Patients with Systemic Lupus Erythematosus

2016 ◽  
Vol 9 ◽  
pp. CMAMD.S32269 ◽  
Author(s):  
Rawhya R. EL-Shereef ◽  
Ahmed Lotfi ◽  
Emad A. Abdel-Naeam ◽  
Heba Tawfik
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Disease Activity ◽  
Renal Biopsy ◽  
Lupus Erythematosus ◽  
Interleukin 6 ◽  
Control Group ◽  
Strong Positive Correlation ◽  
Systemic Lupus ◽  
Kidney Involvement ◽  
Significant Difference

Aim of the Work This study investigates whether serum and urinary interleukin-6 (IL-6) represent an early marker of kidney involvement and assesses the difference between them and renal biopsy in lupus nephritis (LN). Patients and Methods A total of 60 systemic lupus erythematosus (SLE) patients were compared to 20 healthy controls. Urinary and serum IL-6 were measured in both patients and controls. In addition, renal biopsy was done prior or shortly after urine and blood sampling; the results were classified according to the International Society of Nephrology/Renal Pathology Society classification of LN by recording the activity score and chronicity score for each sample. Results There was a significant higher level of urinary IL-6 in the SLE patients with biopsy-proven LN than in those without LN and those of the control group. However, no significant difference was reported between the three groups as regards serum IL-6. A strong positive correlation was found between urinary IL-6 and renal disease activity based on the renal SLE disease activity index (SLEDAI) score with no significant correlation regarding the extra renal SLEDAI. Urinary IL-6 was positively correlated with renal biopsy results and with its activity scores but weakly correlated with the chronicity scores. Conclusion Urinary IL-6 may provide a simple noninvasive potential marker of disease activity of renal involvement in adult patients with SLE.

scholarly journals The Role of Inflammatory, Anti-Inflammatory, and Regulatory Cytokines in Patients Infected with Cutaneous Leishmaniasis in Amazonas State, Brazil

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Thaís Tibery Espir ◽  
Luanda de Paula Figueira ◽  
Maricleide de Farias Naiff ◽  
Allyson Guimarães da Costa ◽  
Marcelo Ramalho-Ortigão ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Expression Profiles ◽  
Control Group ◽  
Tnf Α ◽  
Significant Difference ◽  
Anti Inflammatory ◽  
Amazonas State ◽  
Protein Expression Profiles ◽  
Leishmania Guyanensis

The authors discuss in this paper the role of inflammatory, anti-inflammatory, and regulatory cytokines in patients infected with different species ofLeishmaniain Amazonas State, Brazil. A comparative analysis was made of serum concentrations of these cytokines in the peripheral blood of 33 patients infected with cutaneous leishmaniasis. The isolates were identified asLeishmania guyanensis,L. naiffi, andL. amazonensis. Most (64%) of the patients were male ranging in age from 18 to 58 years. Protein expression profiles of IL-2, IL-4, IL-6, IL-10, IFN-γ, TNF-α, and IL-17 cytokines were shown to vary significantly between infected and noninfected (control group) individuals and according to theLeishmaniaspecies. Infection caused byL. guyanensisaccounted for 73% of the cases and patients with this parasite also showed higher concentrations of IL-2, IFN-γ, IL-4, and IL-17 when compared to infection byL. amazonensis. Patients with infection caused byL. naiffishowed higher concentration of the cytokines analyzed when compared to uninfected patients; however, there was no statistically significant difference with the other species analyzed.

Sign in / Sign up

Export Citation Format

Share Document