scholarly journals Duckweed from a Biorefinery System: Nutrient Recovery Efficiency and Forage Value

Energies ◽  
2020 ◽  
Vol 13 (20) ◽  
pp. 5261
Author(s):  
Marcin Sońta ◽  
Andrzej Łozicki ◽  
Magdalena Szymańska ◽  
Tomasz Sosulski ◽  
Ewa Szara ◽  
...  
Keyword(s):  
Growth Medium ◽  
Nutritional Value ◽  
Liquid Fraction ◽  
Recovery Process ◽  
Nutrient Recovery ◽  
Growth Media ◽  
Fresh Matter ◽  
The Media ◽  
Forage Value ◽  
Interdisciplinary Study

This paper presents the results of an interdisciplinary study aimed at assessing the possibility of using duckweed to purify and recover nutrients from the effluent remaining after struvite precipitation and ammonia stripping from a liquid fraction of anaerobic digestate in a biorefinery located at a Dutch dairy cattle production farm. The nutritional value of duckweed obtained in a biorefinery was assessed as well. Duckweed (Lemna minuta) was cultured on a growth medium with various concentrations of effluent from a biorefinery (EFL) and digested slurry (DS) not subjected to the nutrient recovery process. The study’s results showed that duckweed culture on the media with high contents of DS or EFL was impossible because they both inhibited its growth. After 15 days of culture, the highest duckweed yield was obtained from the ponds with DS or EFL contents in the medium reaching 0.39% (37.8 g fresh matter (FM) and 16.8 g FM per 8500 mL of the growth medium, respectively). The recovery of N by duckweed was approximately 75% and 81%, whereas that of P was approximately 45% and 55% of the growth media with EFL0.39% and DS0.39%, respectively. Duckweed obtained from the biorefinery proved to be a valuable high-protein feedstuff with high contents of α-tocopherol and carotenoids. With a protein content in duckweed approximating 35.4–36.1%, it is possible to obtain 2–4 t of protein per 1 ha from EFL0.39% and DS0.39% ponds, respectively.

scholarly journals Micro Propagation of Pterocarpus santalinoides Using Three Different Growth Media

2019 ◽  
pp. 1-9
Author(s):  
S. J. Ameh ◽  
C. U. Aguoru ◽  
C. C. Iheukwumere ◽  
O. J. Olasan ◽  
U. J. Alfred
Keyword(s):  
Growth Hormone ◽  
Growth Medium ◽  
Basal Medium ◽  
Growth Hormones ◽  
Growth Media ◽  
Ms Medium ◽  
Significant Difference ◽  
Micro Propagation ◽  
The Media ◽  
Pterocarpus Santalinoides

Aims: Micro propagation of P. santalinoides was carried out in order to ascertain the most appropriate culture media for its micro propagation. Study Design: The experiment was laid out in different growth media in the laboratory. Place and Duration of Study: The micro propagation of Pterocarpus santalinoides was carried out at the Tissue culture laboratory of the University of Nigeria, Nsukka and lasted between July and October 2018. Methodology: Seeds from fresh and healthy ripe fruit which was cut open mechanically with the help of secateurs were gotten from Ai-kwu, Otukpa Local Government Area of Benue State, Nigeria. The seeds were air dried and used as explant. The explants were surface sterilized using NaOCl solution for 10 mins, rinsed with distilled water and then the soft seed coat were removed and the seeds were cultured under aseptic conditions on MS medium and other growth medium. Seeds of Pterocarpus santalinoides were inoculated on six different growth media with varying compositions. The media are MS, B5 and white’s without growth hormones (MSoo, B5oo, and WHoo), and each of them was supplemented with 3.0 mgl-1 BAP and 0.5 mgl-1 NAA (MSBN, B5BN, WHBN). Results: Seed germination improved in all the media studied. However, MS combinations gave the best result (90-93%). The maximum number of leaves and roots recorded was in MSBN (3.8 for leaves and 2 for roots) followed by MSoo (2.6) and WHBN (2.6). The leaf area was best for the MS combination (0.232 cm2) followed by the White’s combinations (0.154 cm2) and least for the B5 combinations (0.026 cm2) while shoot and root length was maximum in MSBN (4.28 cm for the shoot and 1.18 cm for the root) followed by WHBN (1.90 cm). The result for t-test revealed that there was a significant difference between the parameters studied for growth media with growth hormones and those without growth hormones. The recorded percentage germination rate for MS medium without growth hormone was 90.75±0.97 while MS medium supplemented with growth hormone was 93.25±0.25. B5 medium without growth medium was 60.25±0.50 and when supplemented with growth hormone, the value was 66.50±0.57. White medium without growth hormone had a value of 75.25±1.70 and when supplemented with growth hormone the value was 78.0±0.81. Conclusion: The growth rates of Pterocarpus santalinoides, in MS medium among other basal media (B5 and White) offers a compromise between all the growth parameters which indicates that variation of the basal medium composition could lead to enhanced Pterocarpus santalinoides regeneration efficiency.

Nutrient Recovery From the Liquid Fraction of Digestate by Clinoptilolite

2017 ◽  
Vol 45 (6) ◽  
pp. 1500153 ◽  
Author(s):  
Nazlı Pelin Kocatürk-Schumacher ◽  
Sander Bruun ◽  
Kor Zwart ◽  
Lars Stoumann Jensen
Keyword(s):  
Liquid Fraction ◽  
Nutrient Recovery

scholarly journals Uji Isolat Bakteri Azotobacter Asal Kebun Lidah Buaya dengan Pupuk Urea terhadap Serapan Nitrogen pada Tanaman Kedelai (Glycine Max L.) di Tanah Gambut

2021 ◽  
Vol 2 ◽  
pp. 131-138
Author(s):  
Nandung Erlanda ◽  
Feira B. Arief ◽  
Ismahan Umran ◽  
Sutarman Gafur ◽  
Denah Suswati
Keyword(s):  
Nitrogen Uptake ◽  
Peat Soil ◽  
Aloe Vera ◽  
Dry Weight ◽  
Growth Media ◽  
Chemical Fertilizers ◽  
Urea Fertilizer ◽  
Randomized Design ◽  
The Media ◽  
Soybean Plants

Azotobacter nitrogen-fixing bacteria and urea fertilizer to reduce excessive use of chemical fertilizers. These bacteria can play a role in increasing nitrogen uptake and further growth of soybean plants. This study aimed to obtain growth media for Azotobacter and increase plant and root growth and nitrogen uptake of Biosoy soybean varieties. The research was conducted to help the uptake of n in the soil and reduce the excessive use of chemical fertilizers. The first experiment was carried out by growing bacteria on the media for 120 hours. The second experiment was designed in a completely randomized design that tested two treatments with additional use of urea fertilizer and Azotobacter and no Azotobacter. The results showed that there was no effect of giving Azotobacter from aloe vera gardens, and urea fertilizer showed a significant effect on the observed variables of soil pH in soybean plants on peat soil. In contrast, the variables observed were population, plant height, N content, N-total, plant dry weight, dry weight of plant roots, and N span of soybean plants had no significant effect.

scholarly journals Comparison of Three Deoxidation Agents for Ozonated Broths Used in Anaerobic Biotechnological Processes

Processes ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 65
Author(s):  
Ewelina Pawlikowska ◽  
Jaroslaw Domanski ◽  
Piotr Dziugan ◽  
Joanna Berlowska ◽  
Weronika Cieciura-Wloch ◽  
...  
Keyword(s):  
Anaerobic Fermentation ◽  
Process Conditions ◽  
Growth Media ◽  
Nutrient Supplementation ◽  
Yeast Biomass ◽  
Residual Oxygen ◽  
The Media ◽  
Biotechnological Processes ◽  
The Subject ◽  
High Concentrations

Anaerobic fermentation of organic compounds is used in many biotechnological processes and has been the subject of much research. A variety of process conditions and different growth media can be used to obtain microbial metabolites. The media must be free from contamination before fermentation. Sterilization is most often achieved by applying heat or other treatments, such as ozonation. Sterilization of liquid media using ozone can be very beneficial, but this method introduces high concentrations of residual oxygen, which inhibit anaerobic processes. Deoxidation is therefore necessary to remove the oxygen from ozonated broths. This study evaluates the effectiveness of three deoxidation agents for two kinds of fermentation media based on malt or molasses: ultrasound, iron(II) sulfate, and Metschnikowia sp. yeast. The time needed for deoxidation varied, depending on the kind of broth and the deoxidation agent. In general, the dynamics of oxygen removal were faster in malt broth. A comparative analysis showed that yeast biomass was the most effective agent, achieving deoxidation in the shortest time. Moreover, the fully deoxidated broth was supplemented with yeast biomass, which is rich in biogenic substrates, expressed as a protein content of 0.13–0.73 g/L. Application of Metschnikowia sp. may therefore be considered as an effective strategy for simultaneous deoxidation and nutrient supplementation of broths used in anaerobic biotechnological processes.

Compositional, Physical, Antioxidant and Sensory Characteristics of Novel Syrup from Date Palm (Phoenix dactylifera L.)

2009 ◽  
Vol 15 (6) ◽  
pp. 583-590 ◽  
Author(s):  
I. Ben Thabet ◽  
S. Besbes ◽  
M. Masmoudi ◽  
H. Attia ◽  
C. Deroanne ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Nutritional Value ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Total Phenolic ◽  
Fresh Matter ◽  
Maple Syrup ◽  
Phoenix Dactylifera L ◽  
Date Palm Sap ◽  
Matter Basis

This study is a contribution to valorise date palm sap (Phoenix dactylifera L.) by elaboration of high quality syrup. Sap was concentrated by evaporation and the obtained product was characterized by its physicochemical, rheological, thermal, sensory properties and by its antioxidant activity. Syrups from date palm sap have a good nutritional value marked by high amounts of sugars (58—75 g/100 g fresh matter basis), minerals (2.1—2.6 g/100 g fresh matter basis) and phenolics (147.61—224.55 mg of ferulic acid equivalents/kg fresh weight). Syrup also presents an antioxidant activity that appears related to total phenolic content. Rheological properties indicate that syrup preserves a Newtonian behavior from 10 °C to 55 °C well modeled by Arrhenius equation. Hedonic evaluation showed that consumers’ appreciation of date palm syrup was not significantly different to the most known sap syrup: maple syrup.

scholarly journals Different Cellular Origins and Functions of Extracellular Proteins from Escherichia coli O157:H7 and O104:H4 as Determined by Comparative Proteomic Analysis

2016 ◽  
Vol 82 (14) ◽  
pp. 4371-4378 ◽  
Author(s):  
Nazrul Islam ◽  
Attila Nagy ◽  
Wesley M. Garrett ◽  
Dan Shelton ◽  
Bret Cooper ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Medium ◽  
Foodborne Pathogen ◽  
The United States ◽  
Extracellular Proteins ◽  
Growth Media ◽  
Environmental Matrices ◽  
Content Type ◽  
E Coli ◽  
Relative Abundances

ABSTRACTExtracellular proteins play important roles in bacterial interactions with the environmental matrices. In this study, we examined the extracellular proteins fromEscherichia coliO157:H7 and O104:H4 by tandem mass spectrometry. We identified 500 and 859 proteins from the growth media ofE. coliO157:H7 and O104:H4, respectively, including 371 proteins common to both strains. Among proteins that were considered specific toE. coliO157:H7 or present at higher relative abundances in O157:H7 medium, most (57 of 65) had secretion signal sequences in their encoding genes. Noticeably, the proteins included locus of enterocyte effacement (LEE) virulence factors, proteins required for peptidyl-lipoprotein accumulation, and proteins involved in iron scavenging. In contrast, a much smaller proportion of proteins (37 of 150) that were considered specific to O104:H4 or presented at higher relative abundances in O104:H4 medium had signals targeting them for secretion. These proteins included Shiga toxin 2 subunit B and O104:H4 signature proteins, including AAF/1 major fimbrial subunit and serine protease autotransporters. Most of the abundant proteins from the growth medium ofE. coliO104:H4 were annotated as having functions in the cytoplasm. We provide evidence that the extensive presence of cytoplasmic proteins inE. coliO104:H4 growth medium was due to biological processes independent of cell lysis, indicating alternative mechanisms for this potent pathogen releasing cytoplasmic contents into the growth milieu, which could play a role in interaction with the environmental matrices, such as pathogenesis and biofilm formation.IMPORTANCEIn this study, we compared the extracellular proteins from two of the most prominent foodborne pathogenicE. coliorganisms that have caused severe outbreaks in the United States and in Europe.E. coliO157:H7 is a well-studied Shiga toxigenic foodborne pathogen of the enterohemorrhagic pathotype that has caused numerous outbreaks associated with various contaminated foods worldwide.E. coliO104:H4 is a newly emerged Shiga toxigenic foodborne pathogen of the enteroaggregative pathotype that gained notoriety for causing one of the most deadly foodborne outbreaks in Europe in 2011. Comparison of proteins in the growth medium revealed significant differences in the compositions of the extracellular proteins for these two pathogens. These differences may provide valuable information regarding the cellular responses of these pathogens to their environment, including cell survival and pathogenesis.

Influence of the bioherbicide phosphinothricin on interactions between phytopathogens and their antagonists

1995 ◽  
Vol 73 (11) ◽  
pp. 1750-1760 ◽  
Author(s):  
Iftikhar Ahmad ◽  
David Malloch ◽  
John Bissett
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Pythium Aphanidermatum ◽  
Phytopathogenic Fungi ◽  
Growth Media ◽  
Trichoderma Species ◽  
Bacterium Bacillus Subtilis ◽  
The Media ◽  
Microbial Toxin ◽  
Bacterium Bacillus

Phosphinothricin is a microbial toxin currently under development as a selective weed killer in the cultivation of transgenic plants engineered to resist its presence. Here it is shown that phosphinothricin is inhibitory to antagonistic soil microorganisms including Bacillus subtilis, Pseudomonas flourescens, and many species of Trichoderma. Phosphinothricin was also inhibitory to the phytopathogenic fungi Rhizoctonia solani and Sclerotinia sclerotiorum. In contrast, phytopathogens Fusarium oxysporum and Pythium aphanidermatum were highly resistant to phosphinothricin. In pathogen–antagonist challenges, species of Trichoderma that parasitize F. oxysporum and P. aphanidermatum were eliminated when phosphinothricin was added to the growth media. Similarly, the ability of Pseudomonas fluorescens to exclude Pythium aphanidermatum was markedly affected by the presence of phosphinothricin in the media. The dominance equilibrium between Trichoderma species and the bacterium Bacillus subtilis was influenced in favor of the former at 0 and 5 mM concentrations of the herbicide and in favor of the latter at 1 mM concentrations. Pseudomonas fluorescens was unable to exclude Trichoderma species regardless of the phosphinothricin concentration in the media. Key words: phosphinothricin, pathogens, fungi, bacteria, antagonists, biocontrol.

Potential Use of Rum Distillery Slops as Animal Feed Supplement I. Mold Growth in Slops.

1969 ◽  
Vol 63 (3) ◽  
pp. 325-329
Author(s):  
Isabel M. González ◽  
Nivia F. Murphy
Keyword(s):  
Nutritional Value ◽  
Animal Feed ◽  
Growth Media ◽  
Mold Growth ◽  
Feed Supplement ◽  
Aspergillus Phoenicis ◽  
Potential Use

Consideration of the nutritional value of slops suggested its use as growth media for the production of fodder molds. Rum distillery slops supported better mold growth than the medium recommended for its growth. Best results were obtained with strain Aspergillus phoenicis isolated from contaminated slops in our laboratory.

scholarly journals Historical and Contemporary NaCl Concentrations Affect the Duration and Distribution of Lag Times from Individual Spores of Nonproteolytic Clostridium botulinum

2007 ◽  
Vol 73 (7) ◽  
pp. 2118-2127 ◽  
Author(s):  
Martin D. Webb ◽  
Carmen Pin ◽  
Michael W. Peck ◽  
Sandra C. Stringer
Keyword(s):  
Growth Medium ◽  
Clostridium Botulinum ◽  
Lag Time ◽  
Lag Phase ◽  
Growth Media ◽  
Sporulation Medium ◽  
Mature Cell ◽  
Inhibitory Effects ◽  
Subsequent Growth ◽  
Lag Times

ABSTRACT In this study we determined the effect of NaCl concentration during sporulation (0 or 3.0% [wt/vol] added NaCl) and subsequent growth (0 or 2.0% [wt/vol] added NaCl) on the distributions of times associated with various stages of the lag phase of individual spores of nonproteolytic Clostridium botulinum strain Eklund 17B. The effects of NaCl on the probability of germination and the probability of subsequent growth were also determined. Spore populations exhibited considerable heterogeneity at all stages of lag phase for each condition tested. Germination time did not correlate strongly with the times for later stages in the lag phase, such as outgrowth and doubling time. Addition of NaCl to either the sporulation or growth media increased the mean times for, and variability of, all the measured stages of the lag phase (germination, emergence, time to one mature cell, and time to first doubling). There was a synergistic interaction between the inhibitory effects of NaCl in the sporulation medium and the inhibitory effects of NaCl in the subsequent growth medium on the total lag time and each of its stages. Addition of NaCl to either the sporulation medium or the growth medium reduced both the probability of germination and the probability of a germinated spore developing into a mature cell, but the interaction was not synergistic. Spores formed in medium with added NaCl were not better adapted to subsequent growth in suboptimal osmotic conditions than spores formed in medium with no added NaCl were. Knowledge of the distribution of lag times for individual spores and quantification of the biovariability within lag time distributions may provide insight into the underlying mechanisms and can be used to improve predictions of growth in food and to refine risk assessments.

COMPARATIVE STUDIES ON THE GROWTH OF MYXOBACTERIA ON BACTERIAL SUSPENSIONS

1963 ◽  
Vol 9 (4) ◽  
pp. 577-584 ◽  
Author(s):  
B. Kletter ◽  
Y. Henis
Keyword(s):  
Growth Medium ◽  
Test Organism ◽  
Growth Media ◽  
Multiplication Rate ◽  
Bacterial Cells ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Negative Organisms ◽  
Myxococcus Virescens ◽  
Number Of Bacteria

The growth of Myxococcus fulvus and Myxococcus virescens on a number of bacteria was followed in a liquid medium. Multiplication of the myxobacteria was accompanied by their adsorption on the bacterial cells, by their coagglutination, and by their adsorption on the glass surface of the culture flask. Lysis of the agglutinated bacterial cells and release of their proteins to the growth medium took place prior to an increase in the lytic activity of the growth medium towards the tested bacteria. Soluble proteins reached a higher level in media containing Gram-negative than in those containing the Gram-positive organisms. No difference was observed in the multiplication rate, sporulation, or pigmentation of the myxobacteria tested, when grown on either Gram-positive or Gram-negative organisms. Using Staphylococcus aureus as a test organism, no antibiotic activity in any of the growth media could be detected.

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