scholarly journals Induction of Defense Responses in Pinus sylvestris Seedlings by Methyl Jasmonate and Response to Heterobasidion annosum and Lophodermium seditiosum Inoculation

Forests ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 628
Author(s):  
Ilze Šņepste ◽  
Baiba Krivmane ◽  
Vilnis Šķipars ◽  
Astra Zaluma ◽  
Dainis E. Ruņģis
Keyword(s):  
Pinus Sylvestris ◽  
Methyl Jasmonate ◽  
Scots Pine ◽  
Insect Pests ◽  
Defense Responses ◽  
Heterobasidion Annosum ◽  
Protective Mechanism ◽  
Seedling Mortality ◽  
Pine Seedlings ◽  
Seedling Inoculation

The induction of defense responses in Pinus sylvestris L. seedlings by methyl jasmonate (MeJA) was investigated in three experiments. Two different MeJA application methods were tested, and induction of defense responses was assayed by seedling inoculation with Heterobasidion annosum (Fr.) Bref. and Lophodermium seditiosum Minter, Staley and Millar. In the first experiment, five-year-old P. sylvestris ramets of one clone were directly treated with MeJA, followed by inoculation with H. annosum. In the second experiment, open-pollinated Scots pine seedlings were treated with MeJA by direct spraying and vaporization, and inoculation with H. annosum was done using a slightly modified protocol. In the third experiment, open-pollinated Scots pine seedlings were treated with MeJA by vaporization and inoculated with L. seditiosum. Direct application of MeJA induced seedling mortality, and in some cases, decreased resistance to inoculation with H. annosum. Application of MeJA by vaporization was less stressful for seedlings, and resulted in increased resistance to both H. annosum and L. seditiosum. In addition, an unforeseen Neodiprion sertifer (Geoffroy) and Hylobius abietis L. infestation provided anecdotal evidence of the efficacy of MeJA in inducing resistance to insect pests as well. Further studies are required on the induction of resistance to additional diseases and pests. Induced resistance could be used as a possible protective mechanism for Scots pine seedlings prior to planting during reforestation of stands to increase vitality and survival.

scholarly journals The Resistance of Scots Pine (Pinus sylvestris L.) Half-sib Families to Heterobasidion annosum

Forests ◽  
2019 ◽  
Vol 10 (3) ◽  
pp. 287 ◽  
Author(s):  
Adas Marčiulynas ◽  
Vaida Sirgedaitė-Šėžienė ◽  
Povilas Žemaitis ◽  
Virgilijus Baliuckas
Keyword(s):  
Phenolic Compounds ◽  
Mortality Rate ◽  
Pinus Sylvestris ◽  
Incidence Rate ◽  
Scots Pine ◽  
Heterobasidion Annosum ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Seedling Mortality ◽  
Sib Families

This study observed the genotypic variation among Scots pine (Pinus sylvestris L.) half-sib families’ susceptibility to Heterobasidion annosum. Scots pine susceptibility was tested in 12 half-sib families by inoculating them with four different H. annosum strains. At two, six, and ten months after the inoculations, the susceptibility indicators (incidence rate, pathogen spread, and mortality rate) were compared and the total phenolic compounds (TPC) in the inoculated and control groups determined using the Folin–Ciocalteu method. Among half-sib families, significant differences were found for seedling mortality (range: 1.3%–21.2%); however, the differences in incidence rate (range: 54%–77%) and pathogen spread (range: 24–53 mm) were not significant. The incidence rate among half-sib families correlated positively and significantly (r = 0.72, p < 0.05) with the mortality rate, while the pathogen spread correlated negatively with mortality, although the correlation was not significant (r = −0.29, p > 0.05). The TPC comparison with susceptibility indicators showed that the half-sib families with lower susceptibility were characterized by the ability to increase TPC after inoculation compared to the control group. This tendency was most apparent in stems and roots six and two months after inoculation, respectively. Correlation analyses revealed that higher TPC in stems six months after inoculation determined a lower incidence rate (r = −0.32, p < 0.05), while higher Change in concentration of total phenolic compounds (TPCΔ) indicated a lower pathogen spread (r = −0.60, p < 0.05). The lowest incidence of the pathogen was determined in half-sib families with the highest TPCΔ in the roots two months after inoculation. The lower susceptibility of Scots pine half-sib families is based on a combination of enhanced constitutive and inducible phenolic defense mechanisms. The data may facilitate the selection of Scots pine half-sib families with low susceptibility for breeding programs and forest management strategies.

scholarly journals Susceptibility of Picea abies and Pinus sylvestris Seedlings of Various origins to Heterobasidion annosum and H. parviporum

2016 ◽  
Vol 70 (1) ◽  
pp. 29-33 ◽  
Author(s):  
Astra Zaļuma ◽  
Arnis Gailis ◽  
Natālija Burņeviča ◽  
Kari Korhonen ◽  
Tālis Gaitnieks
Keyword(s):  
Picea Abies ◽  
Pinus Sylvestris ◽  
Norway Spruce ◽  
Scots Pine ◽  
Fungal Growth ◽  
Seed Source ◽  
Heterobasidion Annosum ◽  
Seed Sources ◽  
Pine Seedlings

Abstract Five-year-old Norway spruce and four-year-old Scots pine seedlings of various origin were inoculated with Heterobasidion annosum s.s. and H. parviporum to estimate whether the susceptibility of seedlings to Heterobasidion was affected by origin of seeds. In total, 520 spruce and 538 pine seedlings from different seed sources and provenance regions of Latvia were tested. Four months after inoculation the fungal growth was measured. The results highlight differences between development of H. annosum and H. parviporum in spruce and pine seedlings. We did not find any seed source that was more resistant than others.

scholarly journals Effect of Prunus serotina Ehrh. Volatile Compounds on Germination and Seedling Growth of Pinus sylvestris L.

Forests ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 846
Author(s):  
Aleksandra Halarewicz ◽  
Antoni Szumny ◽  
Paulina Bączek
Keyword(s):  
Pinus Sylvestris ◽  
Volatile Compounds ◽  
Scots Pine ◽  
Prunus Serotina ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Fraction ◽  
Black Cherry ◽  
Allelopathic Potential ◽  
Pine Seedlings ◽  
Phytotoxic Effect

In temperate European forests invaded by Prunus serotina Ehrh. (black cherry), a reduction in the spontaneous regeneration capacity of Pinus sylvestris L. (Scots pine) is observed. It could be caused by various factors, including allelopathic properties of this invasive plant. In this study the phytotoxic effect of P. serotina volatile compounds on P. sylvestris and the seasonal variation in this effect were assessed. Simple assays showed that volatiles emitted from P. serotina leaves significantly inhibited root growth of P. sylvestris seedlings. Their negative effect on stem growth was much weaker. The strongest phytotoxic effect on Scots pine seedlings was caused by the volatiles emitted from the youngest black cherry leaves. In fresh foliage of P. serotina, nineteen volatile organic compounds were identified by gas chromatography–mass spectrometry (GC–MS). The dominant compound was benzaldehyde. On the basis of tests of linalool alone, it was found that this monoterpene present in the volatile fraction has a strong allelopathic potential and inhibits germination, root elongation and shoot elongation of pine seedlings. The results of our research suggest that volatile compounds from P. serotina leaves could limited survival of P. sylvestris individuals in the seedling phase.

scholarly journals Effect of wood ash mulch on growth of Scots pine seedlings after transplanting into peat soil: A pilot study

2018 ◽  
Vol 64 (No. 1) ◽  
pp. 9-16
Author(s):  
Heiskanen Juha ◽  
Uotila Karri ◽  
Ruhanen Hanna
Keyword(s):  
Scots Pine ◽  
Power Plants ◽  
Peat Soil ◽  
Field Research ◽  
Wood Ash ◽  
Control Treatment ◽  
Seedling Mortality ◽  
Growing Period ◽  
Forest Sites ◽  
Pine Seedlings

Wood ash production from power plants and the use of recycled ash for earthworks and forest peatland fertilization have increased markedly in Finland in recent decades. In this study, effects of wood-based ash on potted Scots pine seedlings were tested in a greenhouse. Seedlings were grown for one to two growing periods in peat with ash mulch thicknesses 0–8 cm. Seedlings grew well in ash thicknesses 0–4 cm during the first growing period. Seedling mortality (60%) occurred with the thickest ash mulch. Soluble nutrients in press water extracts were high compared with the control treatment. N and P levels were suboptimal also with ash mulch. During the second growing period, seedling mortality occurred (17%) already with the thinnest ash mulch. The results suggest that ash mulch around seedlings in quantities of less than 0.5 cm (or 7 kg·m<sup>–2</sup>) is feasible and is not detrimental to Scots pine seedlings. The results provide foundation for further field research on the longer-term impacts of wood ash mulch on planted seedlings on boreal forest sites and on the feasibility testing of the mulch as a supplement to or substitute for the soil preparation for seedling planting.

Effects of mycorrhizas on growth of pine seedlings

2019 ◽  
Vol 28 (03) ◽  
pp. 41-47
Author(s):  
Gantuya B ◽  
Burenjargal O
Keyword(s):  
Survival Rate ◽  
Pinus Sylvestris ◽  
Scots Pine ◽  
Root Length ◽  
Glomus Mosseae ◽  
Root Collar Diameter ◽  
Percentage Survival ◽  
Pine Seedlings ◽  
Collar Diameter ◽  
Root Collar

Scots pine (Pinus sylvestris) is an economically important source of timber in Mongolia and has been widely used in reforestation programs. In this study, we investigated the effects of mycorrhizas on the growth of pine (Pinus sylvestris) seedlings. Inoculation with ectomycorrhizas and endomycorrhizas increased the height, root length, and root collar diameter of the pine seedlings compared to the non-inoculated seedlings. Furthermore, the results of the study showed that the percentage survival rate is higher in mycorrhizal inoculated pine seedlings than control seedlings. These results demonstrated that inoculation could effectively form ectomycorrhizas with pine seedlings and promote its growth, which can be used to restore Mongolian forest. Нарсны тарьцын ургалтанд микоризын үзүүлэх нөлөө Эгэл нарс (Pinus sylvestris) нь Монгол орны хувьд эдийн засгийн чухал ач холбогдолтой мод бөгөөдойжуулалтанд өргөн ашигладаг. Бид энэхүү судалгаагаараа эгэл нарсны (Pinus sylvestris) тарьцныургалтанд микоризaхэрхэн нөлөөлж буйг судлав. Эктомикоризa (Pisolithus tinctorius, Lactarius laccata)болон эндомикоризийн (Glomus mosseae, Glomus spurcum хх) бэлдмэл ашиглан тарьсан тарьцын өндөр,үндэсний хүзүүвчийн диаметр болон үндэсний урт нь микоризын бэлдмэл ашиглаагүй тарьцуудтайхарьцуулахад илүү байв. Түүнчлэн микоризын бэлдмэл ашигласан тарьцуудын амьдралтын хувийнүзүүлэлт хяналтын тарьцуудаас өндөр байгааг уг судалгааны үр дүн харуулав. Дээрх үр дүнгээрмикоризын бэлдмэл нь нарсны суулгацын үндсэнд микориза болон хэлбэржиж, түүний өсөлтийгдэмжиж байгааг илрүүлсэн бөгөөд уг бэлдмэлийг ойг нөхөн сэргээхэд ашиглах боломжтой юм.Түлхүүр үг: Нарс, өндөр, үндэсний урт, диаметр

scholarly journals High Seedling Mortality of Scots Pine Caused by Heterobasidion annosum s.s.

Forests ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1289
Author(s):  
Tuula Piri ◽  
Eeva J. Vainio ◽  
Heikki Nuorteva ◽  
Jarkko Hantula
Keyword(s):  
Root Rot ◽  
Detailed Examination ◽  
Control Measures ◽  
Heterobasidion Annosum ◽  
Fruiting Bodies ◽  
Seedling Mortality ◽  
Secondary Spread ◽  
Clear Cutting ◽  
Pine Seedlings ◽  
Pine Regeneration

This study provides new information on the infection biology and pathogenicity of an important root-rot fungus, Heterobasidion annosum sensu stricto (Fr.) Bref., through a detailed examination of the vegetative spread of clonal individuals and their capacity to produce fruiting bodies on young pine seedlings. The seedlings were planted in a clear-cutting area (c. 1.2 ha in size) after a pine generation that showed slight external symptoms of Heterobasidion root rot. The first dead seedlings were found five years after planting and during a nine-year monitoring period; nearly 600 seedlings were killed by H. annosum s.s. in 48 individual disease centers. Based on pairing tests of 482 isolates, 117 different H. annosum s.s. genotypes were identified. On average, 2.9 genotypes occurred in a single disease center. The extensive secondary spread of genotypes within root systems (up to 48 pine seedlings infected by the same genotype) resulted in annually expanding disease centers. In addition, more than half of the seedlings killed by H. annosum s.s. produced perennial fruiting bodies thus providing air-borne inoculum. The risk of spore infection should be taken into account in any type of cutting operation in young pine stands. Moreover, new control measures directed towards the secondary spread of H. annosum s.s. in pine regeneration are urgently needed in order to maintain the productivity of the pine forest on infested sites.

scholarly journals Application of methyl jasmonate reduces growth but increases chemical defence and resistance against Hylobius abietis in Scots pine seedlings

2005 ◽  
Vol 115 (1) ◽  
pp. 117-124 ◽  
Author(s):  
Juha Heijari ◽  
Anne‐Marja Nerg ◽  
Pirjo Kainulainen ◽  
Heli Viiri ◽  
Martti Vuorinen ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Scots Pine ◽  
Chemical Defence ◽  
Hylobius Abietis ◽  
Pine Seedlings

scholarly journals Influence of toxic metal ions phenols in needles and roots, and on root respiration of Scots pine seedlings

2014 ◽  
Vol 63 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Piotr Karolewski ◽  
Marian J. Giertych
Keyword(s):  
Phenolic Compounds ◽  
Pinus Sylvestris ◽  
Scots Pine ◽  
Salt Concentration ◽  
Root Respiration ◽  
Dark Respiration ◽  
Toxic Metal ◽  
Plant Part ◽  
Pine Seedlings ◽  
One Year

Nitrates of aluminum, cadmium, manganese and lead cause changes in the content of phenolic compounds (o-dPh and TPh) in needles and roots, and in the rate of dark respiration (DR) of roots of one-year-old seedlings of Scots pine (<i>Pinus sylvestris</i> L.). The changes depend on the cation, the salt concentration used, and the analyzed plant part. The observed changes in the levels of phenolic compounds in needles and roots, and the rate of respiration in roots, indicate the following rank in toxicity of the studied metal cations: Mn < Al < Pb < Cd.

scholarly journals Assessing the virulence of ophiostomatoid fungi associated with the pine-infesting weevils to scots pine Pinus sylvestris L. seedlings

2013 ◽  
Vol 66 (2) ◽  
pp. 85-94 ◽  
Author(s):  
Robert Jankowiak
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
Ophiostomatoid Fungi ◽  
Seedling Mortality ◽  
Fungal Virulence ◽  
Ophiostoma Piceae ◽  
Blue Stain ◽  
Leptographium Procerum ◽  
Ophiostoma Floccosum ◽  
Ophiostoma Piliferum

The pine-infesting weevils are known to be effective vectors of ophiostomatoid fungi. To understand more about fungal virulence of these fungi, inoculation studies were conducted on Scots pine (<em>Pinus sylvestris</em> L.). Two-year-old seedlings were wound-inoculated with one of eleven ophiostomatoid fungi associated with pine-infesting weevils. After 11 weeks, a darkened lesion, extending from the point of inoculation, was observed in all species, except for <em>Ophiostoma</em> cf. <em>abietinum</em> Marm. &amp; Butin, <em>Ophiostoma quercus</em> (Georgev.) Nannf., and <em>Sporothrix inflata </em>de Hoog. Seedling mortality was observed in seedlings inoculated with <em>Leptographium truncatum</em> (M.J. Wingf. &amp; Marasas) M.J. Wingf., <em>Leptographium lundbergii</em> Lagerb. &amp; Melin<em>,</em> <em>Leptographium procerum</em> (W.B. Kendr.) M.J. Wingf., <em>Grosmannia radiaticola</em> (J.J. Kim, Seifert &amp; G.H. Kim) Zipfel, Z.W. de Beer &amp; M.J. Wingf., <em>Ophiostoma floccosum</em> Math.-Käärik,<em> Ophiostoma minus</em> (Hedgc.) Syd. &amp; P. Syd., and <em>Ophiostoma piliferum </em>(Fr.) Syd. &amp; P. Syd. <em>Ophiostoma minus</em> and <em>L. truncatum</em> caused the largest lesions and sapwood blue-stain in Scots pine. <em>Grosmannia radiaticola, Ophiostoma piceae </em>(Münch) Syd. &amp; P. Syd., <em>O. floccosum</em>,<em> O. piliferum,</em> <em>L. lundbergii</em>,and<em> L. procerum </em>produced significantly smaller lesions and sapwood blue-stain than <em>O. minus</em> and <em>L. truncatum</em>, while<em> O</em>. cf. <em>abietinum</em>, <em>O. quercus</em> and <em>S. inflata</em> did not cause any lesions.

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