Effect of Ellagic Acid on Fermentation Quality and Bacterial Community of Stylo Silage

This study was conducted to investigate the effect of ellagic acid on the bacterial community and fermentability of stylo silage. Three treatments of stylo silage were used: control (CK) and treated with 1% or 2% ellagic acid (EA1 and EA2) on a fresh matter basis. All silage was stored at ambient temperature and opened on days 3, 7, 14, and 30. Fermentation characteristics, protein fraction, and bacteria community of all periods of silage were analyzed. Results showed that dry matter and crude protein content were increased, and pH value, number of coliform bacteria, contents of acetic acid, and ammonium nitrogen were decreased with the addition of ellagic acid. The antioxidant activity of 1% and 2% ellagic acid treated silages was significantly higher than the control. Meanwhile, the relative abundance of Klebsiella and Clostridium was decreased with the addition of ellagic acid, and the abundance of Lactobacillus, Weissella, and Enterococcus was increased with prolonged days of ensiling. Adding ellagic acid to stylo silage could improve the fermentation quality and preservation of protein, and reduce the abundance of harmful bacteria.

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Effects of Gallic Acid on Fermentation Parameters, Protein Fraction, and Bacterial Community of Whole Plant Soybean Silage

Frontiers in Microbiology ◽

10.3389/fmicb.2021.662966 ◽

2021 ◽

Vol 12 ◽

Author(s):

Cheng Wang ◽

Mingyang Zheng ◽

Shuo Wu ◽

Xuan Zou ◽

Xiaoyang Chen ◽

...

Keyword(s):

Bacterial Community ◽

Gallic Acid ◽

Dry Matter ◽

Ph Value ◽

Amino Nitrogen ◽

Wide Distribution ◽

Coliform Bacteria ◽

Fresh Matter ◽

Whole Plant ◽

Fermentation Parameters

Whole plant soybean (WPS) is a kind of legume resource with characteristics of high nutrition, large biomass, and wide distribution. In the present study, we have investigated the feasibility and effects of gallic acid (GA) on WPS silage quality, nitrogen distribution, tannin content, and bacterial community. The 0.5 and 1% (fresh matter basis) GA were added into WPS for dynamic ensiling (days 3, 7, 14, and 30, respectively). The results showed that the WPS silage with GA addition significantly decreased pH value (6.16–5.38 at ensiling day 30), coliform bacteria count and butyric acid (65.3–62.0 g/kg dry matter at ensiling day 30), and amino nitrogen contents (259–88.2 g/kg total nitrogen at ensiling day 30) and promoted lactic acid (9.62–31.5 g/kg dry matter at ensiling day 30), acetic acid (24.1–85.6 g/kg dry matter at ensiling day 30), and tannin (total phenol and hydrolyzable tannin) contents. Additionally, the GA addition also contributed to the change of bacterial community, where Firmicutes and Lactobacillus were most abundant on phylum and genus levels, respectively. The above results suggested that GA additive applied in WPS silage was an effective strategy to protect nutrition and improve fermentation quality, and the 1% GA addition showed a better effect.

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Study on Microbial Flora's Change and Fermentation Quality in Ensiling of Banana Stems and Leaves

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.524-527.2316 ◽

2012 ◽

Vol 524-527 ◽

pp. 2316-2320 ◽

Cited By ~ 2

Author(s):

Jin Song Yang ◽

Han Lin Zhou ◽

Dong Jin Wang ◽

Hai Sheng Tan ◽

Shao Cheng Xu ◽

...

Keyword(s):

Crude Protein ◽

Dry Matter ◽

Ph Value ◽

Microbial Flora ◽

Aerobic Bacteria ◽

Acid Detergent Fiber ◽

Fermentation Quality ◽

Stems And Leaves ◽

Better Than

Four samples of banana stems and leaves were prepared, which were processed through adding no additives, adding molasses, adding lactic acid bacteria (LAB), and adding both LAB and molasses respectively. In order to find out the regular pattern of microbial flora, microbial flora of each sample was analyzed during ensiling. It was found out that growth of LAB reached its peak of 6.4×107～7.8×109 CFU/g FM in the 6th day of ensiling, growth of aerobic bacteria reached its peak of 2.8×104～5.6×104 CFU/g FM in the 3rd day of ensiling, and growth of yeast reached its peak of 8.6×104～5.8×106 CFU/g FM in the 3rd day of ensiling. All of the samples with different way of processing showed the same trend that growth of microbial flora number were increased gradually and then decreased. It were also found out that decrease of pH value had relation with LAB number, and decrease of pH value were lagged behind the peak number of LAB, and number of aerobic bacteria and yeast were decreased with reproducing of LAB and decline of pH value. Nutrition constituent of forage made of banana stems and leaves was as follows: Dry matter 32.27%～33.59%，Crude protein 7.42%～8.16%, Acid detergent fiber 38.21%～41.43%. Quality of silage added with molasses, LAB and both molasses and LAB were better than that with no additives.

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Co-Occurrence of Regulated and Emerging Mycotoxins in Corn Silage: Relationships with Fermentation Quality and Bacterial Communities

Toxins ◽

10.3390/toxins13030232 ◽

2021 ◽

Vol 13 (3) ◽

pp. 232

Author(s):

Antonio Gallo ◽

Francesca Ghilardelli ◽

Alberto Stanislao Atzori ◽

Severino Zara ◽

Barbara Novak ◽

...

Keyword(s):

Bacterial Community ◽

Bacterial Communities ◽

Dry Matter ◽

Kojic Acid ◽

Corn Silage ◽

Fungal Metabolites ◽

Fusarium Mycotoxins ◽

Low Concentrations ◽

Fermentation Quality ◽

Low Levels

Sixty-four corn silages were characterized for chemicals, bacterial community, and concentrations of several fungal metabolites. Silages were grouped in five clusters, based on detected mycotoxins, and they were characterized for being contaminated by (1) low levels of Aspergillus- and Penicillium-mycotoxins; (2) low levels of fumonisins and other Fusarium-mycotoxins; (3) high levels of Aspergillus-mycotoxins; (4) high levels of non-regulated Fusarium-mycotoxins; (5) high levels of fumonisins and their metabolites. Altersetin was detected in clusters 1, 3, and 5. Rugulusovin or brevianamide F were detected in several samples, with the highest concentration in cluster 3. Emodin was detected in more than 50.0% of samples of clusters 1, 3 and 5, respectively. Kojic acid occurred mainly in clusters 1 and 2 at very low concentrations. Regarding Fusarium mycotoxins, high occurrences were observed for FB3, FB4, FA1, whereas the average concentrations of FB6 and FA2 were lower than 12.4 µg/kg dry matter. Emerging Fusarium-produced mycotoxins, such as siccanol, moniliformin, equisetin, epiequisetin and bikaverin were detected in the majority of analyzed corn silages. Pestalotin, oxaline, phenopirrozin and questiomycin A were detected at high incidences. Concluding, this work highlighted that corn silages could be contaminated by a high number of regulated and emerging mycotoxins.

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Studies of the chemical composition of kales and rapes. I. The kales

The Journal of Agricultural Science ◽

10.1017/s002185960003673x ◽

1959 ◽

Vol 52 (2) ◽

pp. 230-237 ◽

Cited By ~ 11

Author(s):

D. J. C. Jones

Keyword(s):

Crude Protein ◽

Dry Matter ◽

Chemical Constituents ◽

The Other ◽

Winter Period ◽

Crude Fibre ◽

Whole Plant ◽

Phosphorus And Potassium ◽

Plant Basis ◽

Matter Basis

1. Four varieties of marrow stem kale, three of thousand headed kales, Hungry Gap Kale and Rape-Kale were grown at three centres in mid-Wales. They were sampled in the early winter period and separate leaf and stem samples analysed for the proximate constituents and minerals.2. Leaf to stem ratios were measured on both a green and dry matter basis and the latter values used to calculate whole plant values for the chemical constituents.3. The leaves of the marrow stem kales were higher in dry matter than the stems, except in the case of the Purple Stem Kale. This variety resembled the thousand headed and rape-type kales in having a higher dry matter in the stems. On a dry matter basis, the leaves of all the varieties were richer than the stems in ether extract, crude protein, total ash and all the minerals except potassium and sodium. These elements occurred to a greater extent in the stems of the fleshy stemmed marrow stem kales than in the leaves. The stems were higher in crude fibre and nitrogen-free extractives.4. The leaves of the rape-type kales were higher than those of the other varieties in silica, phosphorus and potassium. They were the best source of phosphorus on a whole plant basis. The marrow stem kales had considerably higher leaf and stem values for sodium and were markedly superior to the other varieties as a source of the element.

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Effects of Different Concentrate Feed Proportions on Ruminal Ph Parameters, Duodenal Nutrient Flows and Efficiency of Microbial Crude Protein Synthesis in Dairy Cows During Early Lactation

Animals ◽

10.3390/ani10020267 ◽

2020 ◽

Vol 10 (2) ◽

pp. 267

Author(s):

Katharina Bünemann ◽

Maren Johannes ◽

Rolf Schmitz ◽

Julia Hartwiger ◽

Dirk von Soosten ◽

...

Keyword(s):

Crude Protein ◽

Dry Matter ◽

Individual Variability ◽

Nutrient Digestibility ◽

Group Differences ◽

Early Lactation ◽

Nutrient Flows ◽

Ruminal Ph ◽

Microbial Efficiency ◽

Matter Basis

The aim of the study was to examine different pH parameters, such as variations throughout the day, depending on differing concentrate feed proportions. Moreover, special attention was payed to individual variation in microbial efficiencies (microbial crude protein/fermented organic matter) and their relation to ruminal pH, nutrient flows and digestibilities. For this, cows were grouped according to microbial efficiency (more, n = 5, vs. less efficient cows, n = 4). After calving, thirteen ruminally cannulated pluriparous cows, including nine duodenally cannulated animals, were divided into groups offered rations with a lower (35% on dry matter basis, n = 7) or a higher (60% on dry matter basis, n = 6) concentrate feed proportion. Ruminal pH parameters were assessed continuously by using intraruminal probes. Nutrient flows, nutrient digestibility and microbial efficiency were determined for duodenally cannulated cows. For most ruminal pH parameters it seemed that individual variability was higher than the treatment effect. However, a positive relationship between actual concentrate intake and diurnal pH fluctuations was found. Besides, the effect of individually different microbial efficiencies was assessed. Again, there were no group differences for pH parameters. However, nutrient flows were significantly higher in more efficient cows, whereas digestibilities were lower in in more efficient cows.

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Intake, digestibility, and performance of steers fed diets based on two corn silage hybrids and two concentrate levels

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/s0102-09352010000400016 ◽

2010 ◽

Vol 62 (4) ◽

pp. 868-874 ◽

Cited By ~ 3

Author(s):

F.H.M. Chizzotti ◽

O.G. Pereira ◽

S.C. Valadares Filho ◽

L.O. Tedeschi ◽

K.G. Ribeiro ◽

...

Keyword(s):

Crude Protein ◽

Dry Matter ◽

Average Daily Gain ◽

Corn Silage ◽

Apparent Digestibility ◽

Diet Cost ◽

And Performance ◽

Daily Intakes ◽

Daily Gain ◽

Matter Basis

A trial involving a 2x2 factorial arrangement of treatments was conducted to evaluate two corn silage hybrids (Agromen, AGN35-A42) and (Bayer, A3663) and concentrate levels (25 and 50%) on animal performance. Twenty-four Holstein x Zebu crossbred steers, averaging 335±30kg of BW, were distributed in six randomized blocks to evaluate intake, digestibility, and performance. Treatments consisted of 75% corn silage A + 25% concentrate, 50% corn silage A + 50% concentrate, 75% corn silage B + 25% concentrate, and 50% corn silage B + 50% concentrate (dry matter basis); formulated to be isonitrogenous (13% crude protein, dry matter basis). There were no differences in the daily intakes of dry matter (DM), organic matter (OM), and crude protein. Additionally, there was a concentrate effect on non-fiber carbohydrates and total digestible nutrients intakes, and on total apparent digestibility of DM and OM, with higher intakes for steers fed diets with 50% of concentrate. However, average daily gain (ADG) was not influenced by treatments. The utilization of both corn silage hybrids in association with 25% of concentrate is a good option to feed crossbred steers with ADG close to 1.0kg in order to reduce diet cost.

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A comparison of the effects of liquid manure (urine and water) and nitrogen fertilizers applied to a grass-clover sward on soils of different pH value

The Journal of Agricultural Science ◽

10.1017/s0021859600064108 ◽

1987 ◽

Vol 108 (1) ◽

pp. 17-23 ◽

Cited By ~ 4

Author(s):

M. E. Castle ◽

D. Reid

Keyword(s):

Ammonium Nitrate ◽

Crude Protein ◽

Dry Matter ◽

Ph Value ◽

Total Yield ◽

Nitrogen Fertilizers ◽

Urea Solution ◽

Fertilizer Treatment ◽

Liquid Manure ◽

Clover Content

SummaryIn a 3-year plot experiment on a perennial ryegrass plus white clover sward the pH of the soil on the main plots was adjusted to, and maintained as nearly as possible at, target levels of 5·25, 6·00 and 6·75 by applying either sulphur or lime. Within the main plots the effects on herbage yield, clover content and crude-protein content of applying four fertilizers were compared at nitrogen rates of 225 and 450 kg/ha annually. The fertilizers were liquid manure (urine plus water), prilled urea, a urea solution and an ammonium-nitrate fertilizer. The applications of the urea and the ammonium-nitrate fertilizers were accompanied by dressings of a potassium fertilizer which supplied the same weights of potassium as in the liquid manure. On average the smallest yields of dry matter and crude protein were associated with the lowest soil pH with no significant interactions between pH and fertilizer treatment. The liquid manure gave slightly smaller total yields of mixed herbage dry matter than the other fertilizers, particularly at the low nitrogen rate. The herbage from the liquid manure treatment had a higher clover content and produced a greater total yield of clover dry matter. The effects of the liquid manure on the clover agree with those noted in previous experiments, but no evidence was found that these effects resulted from the liquid manure increasing the pH of the soil and improving the conditions for clover growth. Similarly, no proof was obtained that the clover effects were due to the nitrogen in the liquid manure being wholly in the ammonium form. However, the value of liquid manure as a fertilizer for grassland was demonstrated clearly.

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The effect of lactic acid additive on the quality and chemical composition of meadow grass silage

Biotechnology in Animal Husbandry ◽

10.2298/bah0304061p ◽

2003 ◽

Vol 19 (3-4) ◽

pp. 61-69

Author(s):

Jan Pyś ◽

Wladislaw Migdal ◽

Branislav Zivkovic ◽

Olga Kosovac ◽

Mihal Fabjan ◽

...

Keyword(s):

Lactic Acid ◽

Crude Protein ◽

Dry Matter ◽

Water Solution ◽

Water Soluble ◽

Soluble Carbohydrates ◽

Total N ◽

Grass Silage ◽

Fermentation Quality ◽

Dominant Grasses

In the experiment, forage from the 1st cut of meadow grasses mown at the heading stage of dominant grasses was ensiled. The control silages were made from fresh forage (dry matter 223.5 g?kg-1) and wilted forage (dry matter 351.9 g?kg-1). The experimental silages were made from fresh forages supplemented with a 5% water solution of lactic acid (0.5 and 0.7 l?100 kg-1 of forage) and 8% water solution of lactic acid (0.4 and 0.6 l?100 kg-1 of forage). The highest contents of crude protein, water-soluble carbohydrates and energy were found in silages supplemented with 5% lactic acid applied at 0.7 l?100 kg-1 of forage and in silages supplemented with 8% lactic acid applied at 0.6 l?100 kg-1 of forage. The above silage variants were also characterized by the lowest content of NH3-N in total-N, butyric acid and acetic acid, as well as the highest contents of lactic acid and the best indicators of fermentation quality. No important differences were found between silages made from wilted forage and silages made with lactic acid supplement.

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Effect Of Age And Wholesale Cuts On The Quality Of Indigenous Sheep Carcass

Bangladesh Journal of Animal Science ◽

10.3329/bjas.v37i1.9865 ◽

1970 ◽

Vol 37 (1) ◽

pp. 32-38

Author(s):

MA Hamid ◽

S Akhter ◽

SME Rahman ◽

M Khan

Keyword(s):

Chemical Composition ◽

Crude Protein ◽

Dry Matter ◽

Phosphorus Content ◽

Coliform Bacteria ◽

Meat Sample ◽

Ether Extract ◽

Significant Difference ◽

Total Bacteria

The study was conducted to investigate the nutritive value and microbial status of different wholesale cuts of sheep carcass. The meat sample was obtained from 3 sheep. The ages of sheep were approximately of 1, 2 and 3 years. The wholesale cuts were shoulder, rack, loin and leg of each sheep carcass. The pH, juiciness, chemical composition, total bacteria, coliform bacteria, yeast and mould were studied to assess the quality of meat. The range of pH value were 5.077 - 5.927. The value of juiciness was decreased with the age. The value of dry matter, ash, crude protein, ether extract, calcium and phosphorus content of all samples were ranged from 23.32-30.40%, 0.88-1.027%, 20.50-24.88%, 8.10-12.13%, 0.012-0.038% and 0.048-0.185% respectively. Statistical analysis indicated that the value of pH, juiciness, dry matter, crude protein, ether extract, calcium, phosphorus and yeast (log value) was highly significant (P< 0.01) due to age and cut. Chemical composition, such as dry matter, ash, ether extract and calcium of all the samples increased gradually with the age, except crude protein, which decreased gradually with the age. The phosphorus content of the sample did not differ significantly due to age and cuts. The value of ash content was less significant (P<0.05) due to different cuts but highly significant (P<0.01) due to age. The range of total bacteria and coliform bacteria was 4.210-4.787 and 2.38-3.637/g of sample respectively. The range of yeast was 1.297-2.777/g of sample. Different meat cuts and ages did not show significant difference on total bacteria and coliform bacteria but in case of yeast, the value was highly significant (P<0.01) for the ages and cuts. Nutrient content of mutton varies due to different wholesale cuts as well as age. DOI: http://dx.doi.org/10.3329/bjas.v37i1.9865 BJAS 2008; 37(1): 32-38

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Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing

10.21203/rs.3.rs-29755/v1 ◽

2020 ◽

Author(s):

Zongfu Hu ◽

Deying Ma ◽

huaxin Niu ◽

Jie Chang ◽

Jianhua Yu ◽

...

Keyword(s):

Lactic Acid ◽

Bacterial Community ◽

High Throughput ◽

Bacterial Communities ◽

Crude Protein ◽

High Throughput Sequencing ◽

Ammonium N ◽

Enzyme Combination ◽

Fermentation Quality ◽

Dominant Bacteria

Abstract This study aimed to evaluate the effects of enzymes (cellulase combined with galactosidase),, and the combination of these enzymes with Lactobacillus plantarum (LP) on bacterial diversity using high-throughput sequencing. Alfalfa forages were treated without or with cellulase + ɑ-galactosidase (CEGA), cellulase + LP (CELP), ɑ-galactosidase + LP (GALP). After 56 days of ensiling, All the treated silages exhibited improved fermentation quality as reflecting by decreased pH, ammonium-N and increased lactic acid levels compared to the control silage. Enzymatic treatment improved nutrients value by increased the level of crude protein and decreased the neutral detergent fibre (NDF) level. Treatment of the silage significantly changed the bacterial community, as determined by the PCoA test. LAB dominated the bacterial community of the treated silage after ensiling. The dominant bacteria from Garciella, Enterococcus, Lactobacillus and Pediococcus in control silage changed to Lactobacillus and Pediococcus in CEGA silage, and Lactobacillus in CELP and GALP silages. Collectively, enzymes and enzyme in combination with inoculants both greatly increased the abundance of LAB, with Enterococcus, Lactobacillus and Pediococcus in enzymes only silge (CEGA) and Lactobacillus in enzyme combination with inoculants silage (CELP and GALP).

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