scholarly journals HPLC-DAD-MS and Antioxidant Profile of Fractions from Amontillado Sherry Wine Obtained Using High-Speed Counter-Current Chromatography

Foods ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 131
Author(s):  
Mónica Schwarz ◽  
Fabian Weber ◽  
Enrique Durán-Guerrero ◽  
Remedios Castro ◽  
María del Carmen Rodríguez-Dodero ◽  
...  
Keyword(s):  
High Speed ◽  
Syringic Acid ◽  
Methyl Tert Butyl Ether ◽  
Coumaric Acid ◽  
Complex Matrix ◽  
Butyl Ether ◽  
Solvent Systems ◽  
Polyphenolic Profile ◽  
Counter Current ◽  
First Time

In the present work, the polyphenolic profile of a complex matrix such as Amontillado sherry has been processed by means of high-speed counter-current chromatography (HSCCC) and characterized by HPLC-DAD-MS. An Amberlite XAD-7 column was used to obtain the wine extract, and three different biphasic solvent systems were applied for HSCCC separation: MTBE (methyl tert-butyl ether)/n-butanol/acetonitrile/water (1.1/3/1.1/5+0.1% trifluoroacetic acid), MTBE/n-butanol/acetonitrile/water (2/2/1/5), and hexane/ethyl acetate/ethanol/water (1/5/1/5). As a result, 42 phenolic compounds and furanic derivatives have been identified by means of HPLC-DAD-MS, with 11 of them being identified for the first time in Sherry wines: 3-feruloylquinic acid, isovanillin, ethyl vanillate, furoic acid, dihydro-p-coumaric acid, 6-O-feruloylglucose, ethyl gallate, hydroxytyrosol, methyl protocatechuate, homoveratric acid and veratraldehyde. In addition, the antioxidant capacity (ABTS) of the obtained fractions was determined, revealing higher values in those fractions in which compounds such as gallic acid, protocatechuic acid, protocatechualdehyde, trans-caftaric acid, syringic acid, isovanillin or tyrosol, among others, were present. This is the first time that HSCCC has been used to characterize the phenolic composition of Sherry wines.

scholarly journals Preparative Separation of Diterpene Lactones and Flavones from Andrographis paniculate Using Off-Line Two-Dimensional High-Speed Counter-Current Chromatography

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 620 ◽  
Author(s):  
Xiaowei Sun ◽  
Huijiao Yan ◽  
Yujie Zhang ◽  
Xiao Wang ◽  
Dawei Qin ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
High Speed ◽  
Spectroscopic Methods ◽  
Two Dimensional ◽  
Preparative Separation ◽  
Counter Current ◽  
First Time

Seven diterpene lactones, andrographolide (1), isoandrographolide (2), neo-andrographolide (3), 14-deoxy-11,12-didehydroandrographolide (4), 14-deoxyandrographiside (5), 14-deoxy-11,12-didehydroandrographiside (6), 3,14-dideoxyandrographolide (10), and three flavones, andrographidine C (7), andrographidine A (8), 5-hydroxy-7,8-dimethoxyflavanone (9) have been successfully and efficiently isolated from A. paniculata using an off-line two dimensional (2D) high-speed counter-current chromatography (HSCCC) method for the first time. For the first dimension HSCCC separation, petroleum ether-ethyl acetate-methanol-water 3:7:5:5 (v/v) was employed to isolate 14.4 mg of compound 1, 3.1 mg of compound 2, 7.8 mg of compound 3, and 18.0 mg of compound 4 from 200 mg of the A. paniculata extract. For the second dimension HSCCC separation, petroleum ether-ethyl acetate-methanol-water 2:8:1:9 (v/v) and 5:5:6:4 (v/v) were employed to isolate the collected fractions ranged from 55 to 79 min and the flow out fraction, respectively, which led to 5.1 mg of compound 5, 4.4 mg of compound 6, 2.4 mg of compound 7, 3.3 mg of compound 8, 4.0 mg of compound 9, 7.0 mg of compound 10. The structures of these diterpene lactones and flavones were elucidated by extensive spectroscopic methods.

Three-phase solvent systems for comprehensive separation of a wide variety of compounds by high-speed counter-current chromatography

2006 ◽  
Vol 1133 (1-2) ◽  
pp. 119-125 ◽  
Author(s):  
Yoichi Shibusawa ◽  
Yutaka Yamakawa ◽  
Ryoko Noji ◽  
Akio Yanagida ◽  
Heisaburo Shindo ◽  
...  
Keyword(s):  
High Speed ◽  
Three Phase ◽  
Solvent Systems ◽  
Counter Current

scholarly journals Purification of Flavonoids from Mulberry Leaves via High-Speed Counter-Current Chromatography

Processes ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 91 ◽  
Author(s):  
Pian Zhang ◽  
Kang-Ling Zhu ◽  
Jun Zhang ◽  
Yan Li ◽  
Heng Zhang ◽  
...  
Keyword(s):  
Flow Rate ◽  
Mobile Phase ◽  
High Speed ◽  
Separation Efficiency ◽  
Phase Flow ◽  
Mobile Phase Flow Rate ◽  
Mulberry Leaves ◽  
Mulberry Leaf ◽  
Solvent Systems ◽  
Counter Current

In order to obtain high-purity flavonoid products, the extracts from mulberry leaves were separated and purified via high-speed counter-current chromatography (HSCCC). Moreover, the product was detected via high-performance liquid chromatography (HPLC). The characteristic absorption wavelength of the rutin standard for HSCCC detection and HPLC analysis at 257 nm was tested by ultraviolet scanning analysis. The effect of solvent systems and mobile phase flow rate on the separation efficiency were then researched. Finally, the solvent system of V(ethyl acetate):V(n-butanol):V(water) = 4:1:5 was selected as the operating system for HSCCC. This work theoretically analyzed the impact of the molecular structure and polarity of flavonoids on the choice of solvent systems. The results showed that the mobile phase flow rate had a great influence on the separation efficiency. Furthermore, the separation efficiency increased as the mobile phase flow rate decreased. When the mobile phase flow rate was 5 mL/min, the peak time for flavonoids was 140 min, the retention of the stationary phase was 56.4%, and the purity of the product reached 93.8%. The results of this study greatly improved the purity of flavonoids in mulberry leaf and provided a strong support for the separation and purification of mulberry leaf extract.

scholarly journals Preparative Separation of Alkaloids from Stem of Euchresta tubulosa Dunn. by High-Speed Counter-Current Chromatography Using Stepwise Elution

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4602 ◽  
Author(s):  
Weixin Li ◽  
Huan Wang ◽  
Aiwen Dong
Keyword(s):  
High Speed ◽  
Methylene Chloride ◽  
Liquid Extraction ◽  
Preparative Separation ◽  
Target Compound ◽  
Two Phase ◽  
Chinese Herbal ◽  
Liquid Liquid Extraction ◽  
Counter Current ◽  
First Time

Euchresta tubulosa Dunn. is a Chinese herbal medicine with biological activity, but there are few studies on its components at present. Alkaloids in the stem of Euchresta tubulosa Dunn. were isolated and purified by high-speed counter-current chromatography (HSCCC) using stepwise elution. First of all, liquid-liquid extraction (methylene chloride-methanol-water, 5:1:4, v/v) was used for the preliminary enrichment. According to the partition coefficient (K) of a target compound in a series of different two-phase solvents, the final result was that carbon tetrachloride-methylene chloride-methanol-water (2:3:3:2, v/v) (1) and methylene chloride-methanol-water (5:3:2, v/v) (2) were suitable for the HSCCC using stepwise elution. As a result, the purity was all higher than 93% and matrine (1), oxymatrine (2), N-formyl cytisine (3), and N-acetyl cytisine (4) can be eluted at one time by this mode. Cytisine-type alkaloids were isolated for the first time in this plant. Finally, the applicability of the mode was verified.

scholarly journals HPLC-DAD-APCI-MS as a Tool for Carotenoid Assessment of Wild and Cultivated Cherry Tomatoes

Horticulturae ◽  
2021 ◽  
Vol 7 (9) ◽  
pp. 272
Author(s):  
Lina M. Londoño-Giraldo ◽  
Mónica Bueno ◽  
Eduardo Corpas-Iguarán ◽  
Gonzalo Taborda-Ocampo ◽  
Alejandro Cifuentes
Keyword(s):  
Methyl Tert Butyl Ether ◽  
Cherry Tomato ◽  
Wild Cherry ◽  
Butyl Ether ◽  
Commercial Activities ◽  
Lycopene Content ◽  
Carotenoid Profile ◽  
First Time ◽  
Functional Viewpoint ◽  
Cherry Tomatoes

Carotenoids are naturally occurring fat-soluble pigments found in many organisms. Because of their extensively conjugated carbon–carbon double bond system, carotenoids are potent antioxidants. Although the most abundant carotenoid and best singlet oxygen quencher found in red tomatoes is lycopene, carotenoid profiles may vary between genotypes. The objective of this work was to perform carotenoid profile indentification using HPLC-DAD-APCI-MS in ten wild cherry tomato accessions and one cultivated tomato. A mixture of hexane/acetone/ethanol (50:25:25) and 0.1% BHT was used for carotenoid extraction. For separation, a C30 column at 30 °C with a gradient consisting of methanol, methyl-tert-butyl ether, and water was used for their analysis. Ten major carotenoids were quantified within cherry tomato samples. All accessions present different profiles and quantities of carotenoids. Wild red tomatoes had more lycopene content that commercial tomato, whereas yellow tomatoes present no lycopene. From a functional viewpoint, higher concentrations of carotenoids that could play an antioxidant activity were measured from accessions IAC401, IAC426, LA1480, IAC391, and LA2692. This trait means that these germplasms may be targets for commercial activities. To the best of our knowledge, this is the first time that HPLC-DAD-APCI-MS has been used to analyze these accessions of wild cherry tomatoes that are both functionally promising and suitable for projects with social implementation at a local scale.

scholarly journals High Selective Composite Polyalkylmethylsiloxane Membranes for Pervaporative Removal of MTBE from Water: Effect of Polymer Side-chain

Polymers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1213
Author(s):  
Ilya Borisov ◽  
Ivan Podtynnikov ◽  
Evgenia Grushevenko ◽  
Olga Scharova ◽  
Tatiana Anokhina ◽  
...  
Keyword(s):  
Composite Membrane ◽  
Separation Factor ◽  
Concentration Polarization ◽  
Composite Membranes ◽  
Methyl Tert Butyl Ether ◽  
Side Chain ◽  
Butyl Ether ◽  
Water Separation ◽  
Selective Layer ◽  
First Time

For the first time, the effect of the side-chain in polyalkylmethylsiloxane towards pervaporative removal of methyl tert-butyl ether (MTBE) from water was studied. The noticeable enhancement of separation factor during the pervaporation of 1 wt.% MTBE solution in water through the dense film (40–50 µm) can be achieved by substitution of a methyl group (separation factor 111) for heptyl (161), octyl (169) or decyl (180) one in polyalkylmethylsiloxane. Composite membrane with the selective layer (~8 µm) made of polydecylmethylsiloxane (M10) on top of microfiltration support (MFFK membrane) demonstrated MTBE/water separation factor of 310, which was 72% greater than for the dense film (180). A high separation factor together with an overall flux of 0.82 kg·m−2·h−1 allowed this M10/MFFK composite membrane to outperform the commercial composite membranes. The analysis of the concentration polarization modulus and the boundary layer thickness revealed that the feed flow velocity should be gradually increased from 5 cm·s−1 for an initial solution (1 wt.% of MTBE in water) to 13 cm·s−1 for a depleted solution (0.2 wt.% of MTBE in water) to overcome the concentration polarization phenomena in case of composite membrane M10/MFFK (Texp = 50 °C).

scholarly journals Efficient Separation of Phytochemicals from Muehlenbeckia volcanica (Benth.) Endl. by Polarity-Stepwise Elution Counter-Current Chromatography and Their Antioxidant, Antiglycation, and Aldose Reductase Inhibition Potentials

Molecules ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 224
Author(s):  
Guang-Lei Zuo ◽  
Hyun Yong Kim ◽  
Yanymee N. Guillen Quispe ◽  
Zhi-Qiang Wang ◽  
Seung Hwan Hwang ◽  
...  
Keyword(s):  
Aldose Reductase ◽  
High Speed ◽  
High Performance ◽  
Shikimic Acid ◽  
Aldose Reductase Inhibition ◽  
Mobile Phases ◽  
Wide Range ◽  
Therapeutic Properties ◽  
Counter Current ◽  
First Time

Muehlenbeckia volcanica (Benth.) Endl. (M. volcanica), native to South America, is a traditional Peruvian medicinal plant that has multi-therapeutic properties; however, no phytochemicals have been identified from it yet. In this study, a five-step polarity-stepwise elution counter-current chromatography (CCC) was developed using methanol/water (1:5, v/v) as the stationary phase and different ratios of n-hexane, ethyl acetate, and n-butanol as mobile phases to separate the compounds from the 70% methanol extract of M. volcanica, by which six compounds with a wide range of polarities were separated in a single run of CCC and were identified as gallic acid, protocatechuic acid, 4,4′-dihydroxy-3,3′-imino-di-benzoic acid, rutin, quercitrin, and quercetin. Then, two compounds from the fractions of stepwise elution CCC were separated using conventional high-speed CCC, pH-zone-refining CCC, and preparative high-performance liquid chromatography, and identified as shikimic acid and miquelianin. These compounds are reported from M. volcanica for the first time. Notably, except for shikimic acid, all other compounds showed anti-diabetic potentials via antioxidant, antiglycation, and aldose reductase inhibition. The results suggest that the polarity-stepwise elution CCC can be used to efficiently separate or fractionate compounds with a wide range of polarities from natural products. Moreover, M. volcanica and its bioactive compounds are potent anti-diabetic agents.

Preparative separation of flavonoid glycosides and flavonoid aglycones from the leaves of Platycladus orientalis by REV-IN and FWD-IN high-speed counter-current chromatography

2019 ◽  
Vol 11 (33) ◽  
pp. 4260-4266
Author(s):  
Qian Liu ◽  
Yanling Geng ◽  
Xiao Wang ◽  
Jia Li ◽  
Jinqian Yu
Keyword(s):  
High Speed ◽  
Flavonoid Glycosides ◽  
Preparative Separation ◽  
Flavonoid Aglycones ◽  
Platycladus Orientalis ◽  
Counter Current ◽  
First Time

Three flavonoid glycosides and four flavonoid aglycones were isolated and purified for the first time from the leaves of Platycladus orientalis via two different elution modes of high-speed counter-current chromatography.

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