Co-Culture Strategy of Lactobacillus kefiranofaciens HL1 for Developing Functional Fermented Milk

Our previous studies indicated that Lactobacillus kefiranofaciens HL1, isolated from kefir grain, has strong antioxidant activities and anti-aging effects. However, this strain is difficult to use in isolation when manufacturing fermented products due to poor viability in milk. Thus, the purpose of this study was to apply a co-culture strategy to develop a novel probiotic fermented milk rich in L. kefiranofaciens HL1. Each of four selected starter cultures was co-cultured with kefir strain HL1 in different media to evaluate their effects on microbial activity and availability of milk fermentation. The results of a colony size test on de Man, Rogosa and Sharpe (MRS) agar agar, microbial viability, and acidification performance in MRS broth and skimmed milk suggested that Lactococcus lactis subsp. cremoris APL15 is a suitable candidate for co-culturing with HL1. We then co-cultured HL1 and APL15 in skimmed milk and report remarkable improvement in fermentation ability and no negative impact on the viability of strain HL1 or textural and rheological properties of the milk. Through a co-culture strategy, we have improved the viability of kefir strain HL1 in fermented skimmed milk products and successfully developed a novel milk product with a unique flavor and sufficient probiotics.

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FERMENTED MILK PRODUCT FOR SPORTS NUTRITION

Human Sport Medicine ◽

10.14529/hsm19s117 ◽

2019 ◽

Vol 19 (S1) ◽

pp. 128-136

Author(s):

L Zakharova ◽

I Pushmina ◽

V Pushmina ◽

M Kudriavtsev ◽

S Sitnichuk

Keyword(s):

Whey Protein ◽

Fermented Milk ◽

Sports Nutrition ◽

Starter Cultures ◽

Whey Protein Concentrate ◽

Milk Product ◽

Protein Concentrate ◽

Milk Whey ◽

Milk Whey Protein ◽

Fermented Milk Product

Aim. The article deals with the study of technological approaches to the quality and development of a functional fermented milk product for specialized sports nutrition. Materials and methods. Skim milk, whey protein concentrate, and starter cultures (DELVO-YOG®, DIRECT SET) were used for the study. Bifidobacteria (Bifidobacterium animalis) were introduced as a probiotic. Creatine monohydrate (Creatine Powder C4N9O2N3, USA) was used as an element of sports nutrition. The fruit fillers (“Green Apple”, “Blueberry”, produced by “Zuegg”, Italy) were also introduced into the product. The research was conducted using standard and original methods, mathematical modeling, and statistical processing of experimental data. Results. The obtained data on the physiological effects of proteins, fats, carbohydrates, and nutrients, enhancing the adaptive capacity to the physical and neuro-emotional stress, indicate the need for their use in the technology of specialized food for athletes. Technological approaches to the quality of a functional fermented milk product for sports nutrition were studied. Based on a combination of milk, whey protein concentrate, starter cultures, and bifidobacteria, a fermented milk product was designed. This product is intended for athletes during training, competition, and recovery. The introduction of creatine monohydrate into the milk base allowed enriching the product with amino acids. Conclusion. This study is of great interest in terms of getting yogurts with the textural properties similar to the traditional ones but with the use of functional ingredients. The study also contributes to the development of food technologies and products for sports nutrition.

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Creation of a fermented milk product using the association of probiotic cultures

Food processing industry ◽

10.52653/ppi.2021.8.8.009 ◽

2021 ◽

pp. 36-39

Author(s):

Ирина Владимировна Рожкова ◽

Анна Васильевна Бегунова ◽

Юлия Игоревна Крысанова

Keyword(s):

Salmonella Typhimurium ◽

Lactobacillus Acidophilus ◽

Lactobacillus Reuteri ◽

Lactobacillus Rhamnosus ◽

Fermented Milk ◽

Starter Cultures ◽

Milk Product ◽

Milk Products ◽

Fermented Milk Product ◽

Fermented Milk Products

Кисломолочные продукты приобретают популярность во всем мире благодаря их полезным и функциональным свойствам. Проблема разработки и широкого использования кисломолочных продуктов приобретает огромное значение в период пандемии и экологического кризиса. Полезные свойства кисломолочных продуктов зависят от состава заквасок, используемых для их производства. Вид и количество используемых в составе закваски штаммов обуславливает бифункциональный эффект пробиотического кисломолочного продукта. Из молочнокислых бактерий Lactobacillus являются наиболее изученными пробиотическими микроорганизмами с широким спектром физиолого-биохимических свойств. Для разработки кисломолочного продукта была использована ассоциация заквасочных культур, состоящая из Lactobacillus rhamnosus TR, Lactobacillus reuteri LR1 и Lactobacillus acidophilus H-9 в соотношении 1:6:1, которая обладает функциональным потенциалом. В работе научно обоснованы технологические режимы производства пробиотического кисломолочного продукта c использованием указанной выше ассоциации культур, которая обеспечивает сквашивание пастеризованного обезжиренного молока за 7-8 ч при внесении 5-7 % инокулята, обеспечивая определенные органолептические показатели. При этом количество клеток пробиотических культур составляет для L. acidophilus Н-9 1,1×10 КОЕ/см, для L. reuteri LR 1 (2,1±1,3)×10КОЕ/см, для L. rhamnosus TR (1,37±0,98)×10 КОЕ/см. Показано, что разработанный кисломолочный продукт обладает антагонистической активностью к S. aureus АТСС 6538 и Salmonella typhimurium NCTC 00074, а также антиоксидантной и АПФ-ингибирующей активностью, что характеризует его бифункциональные свойства. Диаметр зоны ингибирования роста S. aureus АТСС 6538 составляет 15,25±0,35 см, а Salmonella typhimurium NCTC 00074 10,5±0,71 см. Антиоксидантная активность разработанного продукта составляет 570,64±18 мкмоль TЭ/мг, а АПФ ингибирующая активность - IC 1,23±1,6 мг белка/см. Fermented milk products are gaining popularity all over the world due to their useful and functional properties. The problem of the development and widespread use of fermented milk products becomes of great importance during a pandemic and an ecological crisis. The beneficial properties of fermented milk products depend on the composition of the starter cultures used for their production. The type and quantity of strains used in the starter culture determine the bifunctional effect of the probiotic fermented milk product. Of the lactic acid bacteria, Lactobacillus is the most studied probiotic genus with a wide range of physiological and biochemical properties. For the development of a fermented milk product, an association of starter cultures was used, consisting of Lactobacillus rhamnosus TR, Lactobacillus reuteri LR1, and Lactobacillus acidophilus H-9 in a ratio of 1: 6:1, which has a functional potential. The work scientifically substantiates the technological modes of production of a probiotic fermented milk product using the above-mentioned association of cultures, which ensures the fermentation of pasteurized skim milk in 7-8 hours with the introduction of 5-7 % inoculum, providing certain organoleptic characteristics. In this case, the cells number of probiotic cultures for L. acidophilus H-9 is 1.1×10 CFU/cm, for L. reuteri LR 1 (2.1±1.3)×10 CFU/cm, for L. rhamnosus TR (1.37±0.98)×10 CFU/cm. It was shown that the developed fermented milk product has an antagonistic activity to S. aureus ATCC 6538 and Salmonella typhimurium NCTC 00074, as well as antioxidant and ACE-inhibiting activity, which characterizes its bifunctional properties. The diameter of the growth inhibition zone for S. aureus ATCC 6538 is 15.25±0.35 cm and for Salmonella typhimurium NCTC 00074 10.5±0.71 cm. The antioxidant activity of the developed product is 570.64±18 μmol TE/mg, and ACE inhibitory activity IC50 1.23±1.6 mg protein/cm.

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A Big World in Small Grain: A Review of Natural Milk Kefir Starters

Microorganisms ◽

10.3390/microorganisms8020192 ◽

2020 ◽

Vol 8 (2) ◽

pp. 192 ◽

Cited By ~ 5

Author(s):

Fatemeh Nejati ◽

Stefan Junne ◽

Peter Neubauer

Keyword(s):

Environmental Parameters ◽

Fermented Milk ◽

Starter Cultures ◽

Milk Product ◽

Microbial Composition ◽

Kefir Grain ◽

Kefir Grains ◽

Natural Milk ◽

The Impact ◽

Fermented Milk Product

Milk kefir is a traditional fermented milk product whose consumption is becoming increasingly popular. The natural starter for kefir production is kefir grain, which consists of various bacterial and yeast species. At the industrial scale, however, kefir grains are rarely used due to their slow growth, complex application, bad reproducibility and high costs. Instead, mixtures of defined lactic acid bacteria and sometimes yeasts are applied, which alter sensory and functional properties compared to natural grain-based milk kefir. In order to be able to mimic natural starter cultures for authentic kefir production, it is a prerequisite to gain deep knowledge about the nature of kefir grains, its microbial composition, morphologic structure, composition of strains on grains and the impact of environmental parameters on kefir grain characteristics. In addition, it is very important to deeply investigate the numerous multi-dimensional interactions among different species, which play important roles on the formation and the functionality of grains.

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Evaluation of Some Factors with Significant Influence on Functionality and Viability of Probiotic Strains LA-5 Â® and L. Casei 431 Â® in Multiple Starter Cultures in Mesophilic Fermented Milk

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Agriculture ◽

10.15835/buasvmcn-agr:8783 ◽

2012 ◽

Vol 69 (2) ◽

Author(s):

Daniela PARASCHIV ◽

Aida VASILE

Keyword(s):

Lactococcus Lactis ◽

Fermented Milk ◽

Starter Cultures ◽

Milk Product ◽

Lactococcus Lactis Subsp ◽

Commercial Code ◽

Long Time ◽

Probiotic Strains

Abstract.Â For a very long time, probiotics have been selected on the basis of their suitability to foodâ€™s environment and technological procedures, as well as survival ability in vitro and also in vivo. These criteria are still used. The aim of this study was to select, using the Plakett-Burman design of experiments model, of three significant factors, which influence the multiplication ability and viability of probiotic strains Lactobacillus acidophilus (commercial code LA-5Â®) and Lactobacillus casei subsp. paracasei (commercial code L. casei 431Â®) in fermented mesophilic milk product (commercial named Sana) obtained by fermentation with a multiple culture with mesophilic lactic starter Flora Danica Chr. Hansen commercial starters (Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. diacetylactis and Leuconostoc mesenteroides subsp. cremoris). By statistical analysis the most important factors (independent variables)Â with influence on the probiotic bacteria were selected for the optimized. These are the temperature of fermentation; the ratio between probiotic strains and Flora Danica and the addition of prebiotic Fagopyrum esculentum flour. In the future experiments the optimum values of these parameters will be optimized by using response surface methodology, in order to establish the conditions in which can be obtain the mesophilic fermented milk products with probiotic action in vivo.Â

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Effects of Fructose and Oligofructose Addition on Milk Fermentation Using Novel Lactobacillus Cultures to Obtain High-Quality Yogurt-like Products

Molecules ◽

10.3390/molecules26195730 ◽

2021 ◽

Vol 26 (19) ◽

pp. 5730

Author(s):

Dorota Zielińska ◽

Katarzyna Marciniak-Lukasiak ◽

Marcelina Karbowiak ◽

Piotr Lukasiak

Keyword(s):

Sensory Quality ◽

Microbiological Quality ◽

Fermented Milk ◽

Quality Attributes ◽

Starter Cultures ◽

Fermented Foods ◽

Milk Products ◽

Milk Fermentation ◽

Fermented Milk Products ◽

Traditional Fermented Foods

The incorporation of prebiotics in fermented milk products is one of the best ways to promote health benefits while improving their sensory characteristics at the same time. The aim of this study was to evaluate the effects of the addition of fructose and oligofructose (1% and 2%) on the physicochemical, rheological, sensory, and microbiological quality attributes of fermented milk products inoculated with indigenous probiotic starter cultures of Lactobacillus isolated from Polish traditional fermented foods. The samples were evaluated during 35 days of refrigerated storage. The oligofructose and fructose caused increases in the populations of bacteria in comparison to the control fermented milk products without the addition of saccharides. The degrees of acidification in different fermented milk samples, as well as their viscosity, firmness, syneresis, and color attributes, changed during storage. The highest overall sensory quality levels were observed for the samples supplemented with L. brevis B1 and oligofructose. This study is the first attempt to compare the influences of different sugar sources on the physicochemical, rheological, sensory, and microbiological quality attributes of fermented milk products.

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Properties of Lactic Acid Microorganisms: Long-Term Preservation Methods

Food Processing Techniques and Technology ◽

10.21603/2074-9414-2018-4-30-38 ◽

2019 ◽

Vol 48 (4) ◽

pp. 30-38 ◽

Cited By ~ 1

Author(s):

Ольга Кригер ◽

Olga Kriger ◽

Светлана Носкова ◽

Svetlana Noskova

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Milk Production ◽

Freezing Temperature ◽

Fermented Milk ◽

Starter Cultures ◽

Residual Pressure ◽

Milk Product ◽

Multienzyme Complexes

Among the relevant studies on lactic acid bacteria there are projects devoted to the multienzyme complexes of starter cultures, new competitive bacterial concentrates and their use in fermented functional milk products. In fermented milk production, the process of albuminolysis has a significant impact on the consistency, taste, and smell of the product. Therefore, lactic acid bacteria with high proteolytic properties are of the greatest interest for fermented milk industry. The present research features long- term methods for preservation of the properties of lactic acid microorganisms. The experiment defined the regime parameters of combined starter lyophilisation. The results prove that fermented milk production requires high-quality starter strains. The authors developed a long-term method for preservation of properties of particular strains of lactic acid microorganisms. The method presupposes freeze-drying with the following parameters: freezing temperature – minus 25°C in a protective 5%-glycerol medium (90 minutes); the drying temperature – 30°C (6 hours); refrigerating load – 5.45 kW/m²; residual pressure – 0.6‒0.8 kPa, bed depth – 2 mm. The authors also developed the necessary documentation (No. 9225-096-02054145-2013), procedures, and formula of the fermented milk product with a combined direct application starter.

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Application of starter cultures on milk and milk-containing mixtures

Proceedings of the Voronezh State University of Engineering Technologies ◽

10.20914/2310-1202-2021-2-102-107 ◽

2021 ◽

Vol 83 (2) ◽

pp. 102-107

Author(s):

E. V. Ivanova ◽

N. V. Romanova ◽

O. Y. Ilina

Keyword(s):

Milk Fat ◽

Raw Materials ◽

Starter Culture ◽

Food Additives ◽

Meat Products ◽

Fermented Milk ◽

Starter Cultures ◽

Milk Product ◽

Organoleptic Properties ◽

Dairy Proteins

Milk-containing products occupy a large place in the range of popular products. Replacing milk fat or protein with vegetable fat can reduce the biological value of the product and its organoleptic properties. the effect of direct application starter cultures on raw materials that use functionally necessary ingredients (structure-forming agents, salt, sugar, etc.), food additives (thickeners, stabilizers, preservatives, etc.) and a variety of non-dairy components (non-dairy fats, non-dairy proteins, meat products, fruits, vegetables, spices, herbs, nuts, etc.) is ambiguous. The paper deals with the use of homogeneous and combined starter cultures in the production of fermented milk products from natural and normalized milk and milk-containing mixtures with a substitute for milk fat. The introduction of components of plant origin into the product leads to a decrease in the content of milk protein in the mixtures, which in turn leads to the formation of clots with a non-uniform consistency. Slow acid formation in milk-containing mixtures indicates a decrease in milk sugar in them with this type of normalization in fat, relative to the normalized milk according to the classical technology. This pattern was revealed both when using starter cultures on lactic acid thermophilic streptococci, and on combined starter cultures. In this paper, it is proposed to use Streptococcus salivarius subsp in ferments for milk-containing mixtures. thermophilus strain ST-95. The introduction of the ST-95 strain into the starter culture made it possible to obtain from the milk-containing mixture a fermented milk product with well-pronounced organoleptic properties, a stable clot structure and sufficiently high taste properties. The greatest positive effect was obtained on combined ferments.

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Preparation of low-lactose fermented milk products for baby food

Food processing industry ◽

10.52653/ppi.2021.6.6.001 ◽

2021 ◽

pp. 23-26

Author(s):

Татьяна Алексеевна Антипова ◽

Светлана Валерьевна Фелик ◽

Надежда Леонидовна Андросова ◽

Сергей Владимирович Симоненко

Keyword(s):

Lactococcus Lactis ◽

Lactobacillus Acidophilus ◽

Fermentation Process ◽

Fermented Milk ◽

Starter Cultures ◽

Lactobacillus Bulgaricus ◽

Lactose Hydrolysis ◽

Milk Product ◽

Lactococcus Lactis Subsp ◽

Lactose Fermentation

Статья посвящена получению кисломолочного продукта для питания детей с лактазной недостаточностью. Одним из наиболее распространенных способов получения низколактозных продуктов является применение гидролиза лактозы с использованием ферментных препаратов. Целью работы было изучение процесса сквашивания низколактозного молочного продукта. Учитывая, что при использовании методов ферментирования лактозы продукт приобретает выраженный сладкий вкус, обусловленный наличием глюкозы, требовалось установить заданное количество лактозы при достижении оптимальных органолептических показателей. Результаты исследований свидетельствуют об изменении органолептических и физико-химических показателей образцов в результате гидролиза лактозы. Вкусовые проявления сладости в продукте появляются при степени гидролиза лактозы 50 % и усиливаются при дальнейшем проведении гидролиза. Для проведения процесса сквашивания применяли следующие виды заквасочных культур: Lactobacillus acidophilus, Treptococcus thermophilus, Lactobacillus bulgaricus, Streptococcus thermophiles, Lactococcus lactis subsp. lactis и Lactococcus lactis subsp. Сremoris. Процесс сквашивания во всех образцах низколактозного молока имеет свои особенности, характерные для используемой заквасочной культуры. Следует отметить, что заквасочные культуры, включающие ацидофильную палочку, интенсифицируют процесс сквашивания исследуемых образцов. Содержание лактозы после окончания процесса сквашивания в исследуемых образцах составило 1,35; 1,4 %, что соответствует требованиям ТР ТС 027/2012 «О безопасности отдельных видов специализированной пищевой продукции, в том числе диетического лечебного и диетического профилактического питания». The article is devoted to the preparation of a fermented milk product for the nutrition of children with lactase deficiency. One of the most common methods of obtaining low-lactose products is the use of lactose hydrolysis using enzyme preparations. The aim of the work was to study the process of fermentation of low-lactose dairy product. Given that when using the methods of lactose fermentation, the product acquires a pronounced sweet taste due to the presence of glucose, it was necessary to set a given amount of lactose when achieving optimal organoleptic parameters. The results of the studies indicate a change in the organoleptic and physico-chemical parameters of the samples as a result of lactose hydrolysis. The taste manifestations of sweetness in the product appear at the degree of lactose hydrolysis of 50 % and increase with further hydrolysis. The following types of starter cultures were used for the fermentation process: Lactobacillus acidophilus; Treptococcus thermophilus, Lactobacillus bulgaricus; Streptococcus thermophiles; Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. Cremoris. The fermentation process in all samples of low-lactose milk has its own characteristics characteristic of the starter culture used. It should be noted that starter cultures, including Acidophilus bacillus, intensify the process of fermentation of the studied samples. The lactose content after the end of the fermentation process in the studied samples was 1.35; 1.4 %, which corresponds to the requirements of TR CU 027/2012 «On the safety of certain types of specialized food products, including dietary therapeutic and dietary preventive nutrition».

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