The In Vivo Antioxidant and Hepatoprotective Actions of Selected Chinese Teas

Tea is a popular beverage and shows very strong in vitro antioxidant activity. However, the relationship among in vitro and in vivo antioxidant activities in teas is seldom reported. In this study, in vivo antioxidant and hepatoprotective activities of 32 selected Chinese teas were evaluated on a mouse model with acute alcohol-induced liver injury. The results showed that most teas significantly reduced the levels of alanine transaminase, aspartate transaminase, alkaline phosphatase, triacylglycerol, and total bilirubin in the sera of mice at a dose of 400 mg/kg. In addition, most teas greatly decreased the malondialdehyde level and increased the levels of superoxide dismutase, glutathione peroxidase, and glutathione in the liver of mice, indicating the antioxidant and hepatoprotective activities of teas. Furthermore, the in vivo antioxidant activity of dark tea was stronger than that of green tea, opposite to the results of the in vitro study. Among these 32 teas, Black Fu Brick Tea, Pu-erh Tea, and Qing Brick Tea showed the strongest antioxidant and hepatoprotective activities. Moreover, total phenolic content as well as the contents of epicatechin, gallocatechin gallate, and chlorogenic acid were found to contribute, at least partially, to the antioxidant and hepatoprotective actions of these teas. Overall, teas are good dietary components with antioxidant and hepatoprotective actions.

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Estimation of Total Phenolics and Flavonoid Content and In-vitro Antioxidant Activity of Drypetes sepiaria Stem (Wight and Arn.) Pax and K. Hoffm.

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v5i1.8865 ◽

2015 ◽

Vol 5 (1) ◽

Author(s):

Packia M. ◽

Daffodil D. ◽

Tresina S. ◽

Mohan R.

Keyword(s):

Antioxidant Activity ◽

Total Phenolics ◽

Antioxidant Activities ◽

Reducing Power ◽

Model Systems ◽

Aluminium Chloride ◽

Total Phenolic ◽

Ethanol Extracts ◽

In Vitro Antioxidant Activity

The total phenolics, flavonoids and in vitro antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extracts of stem of Drypetes sepiaria were determined using various antioxidant model systems viz, DPPH, hydroxyl, superoxide, ABTS and reducing power. Total phenolic content was estimated by Folin-Ciocalteau method. Flavonoids were determined by Aluminium chloride method. The total phenolics and flavonoids contents were found to be 0.81 g 100 g-1 and 1.12g 100 g-1 respectively in the methanol extract. Among the solvent tested, methanol and ethanol extracts of D. sepiaria stem showed potent in vitro antioxidant activities. This investigation explored that D. sepiaria stem is a potential source of natural antioxidant.

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QUANTITATIVE ANALYSIS OF NATURAL ANTIOXIDANTS AND FREE RADICAL SCAVENGING ACTIVITIES OF CRUCIFERAE VEGETABLES

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i4.17159 ◽

2017 ◽

Vol 10 (4) ◽

pp. 470

Author(s):

Jamuna K S ◽

Ramesh C K ◽

Aditya Rao Sj ◽

Paramesha M ◽

Riaz Mahmood

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Extraction Procedure ◽

Radical Scavenging ◽

Natural Antioxidants ◽

Plant Materials ◽

Cruciferous Vegetable ◽

The Relationship ◽

In Vitro Antioxidant Activity

Objective: The aim of this research program is to evaluate the antioxidant potentialities comprehensively utilizing commonly available cruciferous vegetables of India, viz., cabbage, cauliflower, kohlrabi, and radish.Methods: The plant materials were procured and processed for cold extraction procedure using 70% ethanol. The extracts were primarily assessedfor their phytoconstituents and further for their in vitro antioxidant activity using various qualitative and quantitative estimations. The results of quantitative estimations were expressed in terms of equivalence with respective standards, and the antioxidant potentiality of various scavenging and chelating activities were expressed in terms of 50% effective concentration. Further, correlation studies were made between quantitative and qualitative assays to study the relationship between the effects of different phytoconstituents groups.Results: In this study, the results revealed that all the four cruciferous vegetable extracts possess potential antioxidant activities. Among the extractsunder study kohlrabi has recorded superior antioxidant potential than others. It is clear from the study that the tested cruciferous vegetablesmanifested differential expression of antioxidant capacity due to their phytoconstituents.Conclusion: From the results, it can be concluded that the Cruciferae vegetables have potent antioxidant activity contributing to the use for healthbenefits in addition to their nutritive role as vegetable.Keywords: Cruciferae vegetables, Phytochemicals, In vitro antioxidant activity, Correlation.

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Influence of the Storage in Bottle on the Antioxidant Activities and Related Chemical Characteristics of Wine Spirits Aged with Chestnut Staves and Micro-Oxygenation

Molecules ◽

10.3390/molecules27010106 ◽

2021 ◽

Vol 27 (1) ◽

pp. 106

Author(s):

Sheila Oliveira-Alves ◽

Sílvia Lourenço ◽

Ofélia Anjos ◽

Tiago A. Fernandes ◽

Ilda Caldeira ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Total Phenolic ◽

Chemical Characteristics ◽

Low Molecular Weight ◽

Nitrogen Control ◽

Frap Assay ◽

Phenolic Index ◽

In Vitro Antioxidant Activity

Different ageing technology of wine spirits (WSs) has been investigated, but little has been published on the chemical evolution of aged WS during storage in bottle. The purpose of this study was to examine how 12 months of storage in bottle affected the evolution of antioxidant activity (DPPH, FRAP and ABTS assays), total phenolic index (TPI) and low molecular weight (LMW) compounds content of the WSs aged through alternative technology using three micro-oxygenation levels (MOX) and nitrogen control (N). Results revealed the ability of phenolic compounds from aged WSs to scavenge free radicals during storage in bottle. Among the in vitro antioxidant-activity methods, FRAP assay was the more effective to differentiate WSs according to the ageing technology. Concerning the overall influence of storage in bottle on antioxidant activity, and TPI and LMW compounds content, the higher results were obtained for the MOX modalities (O15, O30 and O60), which showed a similar evolution. In summary, this study provides innovative information, demonstrating that the differences between the aged WSs imparted throughout the ageing process (resulting from different MOX levels) were mostly retained, and only slight modifications during storage in bottle were found.

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Antioxidant and genotoxic properties of Maytenus dasyclada: a comparative study in relation to Maytenus reference species

Brazilian Journal of Biology ◽

10.1590/1519-6984.18113 ◽

2015 ◽

Vol 75 (2) ◽

pp. 471-476 ◽

Cited By ~ 3

Author(s):

RL. Cansian ◽

GB. Kubiak ◽

L. Borsatti ◽

AA. Mielniczki-Pereira ◽

SS. Roman ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Pearson Correlation ◽

Total Phenolic ◽

Reference Species ◽

Carbonyl Content ◽

Ic50 Values ◽

In Vitro Antioxidant Activity

In these work the in vitro antioxidant activity and the in vivo genotoxicity of M. dasyclada was compared to the reference species M. aquifolium and M. ilicifolia. M. dasyclada showed in vitro antioxidant activity comparable to M. aquifolium but lower than M. ilicifolia, being that a inverse Pearson correlation between DPPH IC50 values and total phenolic content was detected (–0.932). The carbonyl content of M. dasyclada and M. aquifolium extracts promoted a similar increase in protein oxidation in vivo, while M. ilicifolia no altered the carbonyl levels. The comet assay demonstrated that the three analyzed species promoted a low and similar level of genotoxicity; which is compatible with DNA damage induced by other medicinal plants and is partially recovered by a co-treatment with vitamin C. The data showed M. dasyclada as antioxidant activity in vitro, and that its genotoxic and pro-oxidant effects in vivo are comparable to the Maytenus reference species.

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In vitro antioxidant activity, total phenolic and flavonoid contents of aqueous and alcoholic extract of bilwadi agad

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2020.sp1.184 ◽

2020 ◽

Vol 12 (sp1) ◽

Keyword(s):

Antioxidant Activity ◽

Total Phenolic ◽

Alcoholic Extract ◽

Phenolic And Flavonoid ◽

In Vitro Antioxidant Activity

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Determination of in vitro antioxidant activity and in vivo antineoplastic effects against Ehrlich ascites carcinoma of methanolic extract of Sphagneticola calendulacea (L.) Pruski

Heliyon ◽

10.1016/j.heliyon.2021.e07228 ◽

2021 ◽

pp. e07228

Author(s):

Md. Wasim Bari ◽

Ariful Islam ◽

Md. Monirul Islam ◽

Mst Julia Sultana ◽

Rashida Afroz ◽

...

Keyword(s):

Antioxidant Activity ◽

Methanolic Extract ◽

Ehrlich Ascites Carcinoma ◽

Ehrlich Ascites ◽

Sphagneticola Calendulacea ◽

In Vitro Antioxidant Activity

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Evaluation of Antioxidant Properties ofPhaulopsis fascisepalaC.B.Cl. (Acanthaceae)

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/nem098 ◽

2009 ◽

Vol 6 (2) ◽

pp. 227-231 ◽

Cited By ~ 28

Author(s):

S. A. Adesegun ◽

A. Fajana ◽

C. I. Orabueze ◽

H. A. B. Coker

Keyword(s):

Ascorbic Acid ◽

Leaf Extract ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Chelating Ability

The antioxidant activities of crude extract ofPhaulopsis fascisepalaleaf were evaluated and compared with α-tocopherol and BHT as synthetic antioxidants and ascorbic acid as natural-based antioxidant.In vitro, we studied its antioxidative activities, radical-scavenging effects, Fe2+-chelating ability and reducing power. The total phenolic content was determined and expressed in gallic acid equivalent. The extract showed variable activities in all of thesein vitrotests. The antioxidant effect ofP. fascisepalawas strongly dose dependent, increased with increasing leaf extract dose and then leveled off with further increase in extract dose. Compared to other antioxidants used in the study, α-Tocopherol, ascorbic acid and BHT,P. fascisepalaleaf extract showed less scavenging effect on α,α,-diphenyl-β-picrylhydrazyl (DPPH) radical and less reducing power on Fe3+/ferricyanide complex but better Fe2+-chelating ability. These results revealed thein vitroantioxidant activity ofP.fascisepala.Further investigations are necessary to verify these activitiesin vivo.

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An Overview of Structural Aspects and Health Beneficial Effects of Antioxidant Oligosaccharides

Current Pharmaceutical Design ◽

10.2174/1381612824666180517120642 ◽

2020 ◽

Vol 26 (16) ◽

pp. 1759-1777 ◽

Cited By ~ 1

Author(s):

Tatiane F. Vieira ◽

Rúbia C. G. Corrêa ◽

Rosely A. Peralta ◽

Regina F. Peralta-Muniz-Moreira ◽

Adelar Bracht ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Sugar Content ◽

Animal Health ◽

Monosaccharide Composition ◽

Degree Of Polymerization ◽

Chemical Diversity ◽

In Vivo Studies

Background: Non-digestible oligosaccharides are versatile sources of chemical diversity, well known for their prebiotic actions, found naturally in plants or produced by chemical or enzymatic synthesis or by hydrolysis of polysaccharides. Compared to polyphenols or even polysaccharides, the antioxidant potential of oligosaccharides is still unexplored. The aim of the present work was to provide an up-to-date, broad and critical contribution on the topic of antioxidant oligosaccharides. Methods: The search was performed by crossing the words oligosaccharides and antioxidant. Whenever possible, attempts at establishing correlations between chemical structure and antioxidant activity were undertaken. Results: The most representative in vitro and in vivo studies were compiled in two tables. Chitooligosaccharides and xylooligosaccharides and their derivatives were the most studied up to now. The antioxidant activities of oligosaccharides depend on the degree of polymerization and the method used for depolymerization. Other factors influencing the antioxidant strength are solubility, monosaccharide composition, the type of glycosidic linkages of the side chains, molecular weight, reducing sugar content, the presence of phenolic groups such as ferulic acid, and the presence of uronic acid, among others. Modification of the antioxidant capacity of oligosaccharides has been achieved by adding diverse organic groups to their structures, thus increasing also the spectrum of potentially useful molecules. Conclusion: A great amount of high-quality evidence has been accumulating during the last decade in support of a meaningful antioxidant activity of oligosaccharides and derivatives. Ingestion of antioxidant oligosaccharides can be visualized as beneficial to human and animal health.

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