scholarly journals Sensory Changes and Listeria monocytogenes Behavior in Sliced Cured Pork Loins during Extended Storage

Foods ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 621
Author(s):  
Rita Silva ◽  
Jorge Pereira ◽  
Margarida Rouxinol ◽  
Luis Patarata
Keyword(s):  
Listeria Monocytogenes ◽  
Shelf Life ◽  
Challenge Test ◽  
Meat Products ◽  
Consumer Acceptance ◽  
Prolonged Storage ◽  
Wine Aroma ◽  
Pork Meat ◽  
Sensory Changes ◽  
Microbial Challenge

Cured pork loins are sausages with a production tradition in several regions worldwide. They are made from one of the noblest cuts of pork, and for this reason cured loins are one of the most expensive pork meat products. Establishing the correct shelf life allows products to be accepted by the consumer, and to avoid the costs associated with shorter shelf lives. The aim of this study is: (1) to establish proper shelf life by evaluating the willingness of participants to consume and the sensory modifications that occur during prolonged storage via Check All That Apply (CATA) questions; and (2) to study the behavior of Listeria monocytogenes through a microbial challenge test. Sliced cured pork loins can be stored at 6 ± 1 °C for 105 days while maintaining a consumer acceptance of more than 75%. The freshness loss was associated mainly with a decrease in aromatic notes (particularly the smoke and cured aroma), and with the appearance of spoiled characteristics, specifically a sour/vinegar aroma and acidic taste that were detected by a reduced proportion of participants. The freshness evaluation was positively influenced by the typical characteristics of cured products, such as color and a garlic and wine aroma. Sour/vinegar aroma and acidic taste were the attributes most associated with higher freshness penalization. During the period of the test, Listeria monocytogenes inoculated onto the cured loin slices did not grow.

scholarly journals Microbiological Safety and Organoleptic Quality of Homogenized Sausages Manufactured with Commercial Functional Additives

2021 ◽  
Vol 11 (24) ◽  
pp. 11662
Author(s):  
Anna Fudali ◽  
Iwona Chełmecka ◽  
Anna Marietta Salejda ◽  
Grażyna Krasnowska
Keyword(s):  
Listeria Monocytogenes ◽  
Meat Products ◽  
Consumer Acceptance ◽  
Organoleptic Quality ◽  
Microbiological Safety ◽  
Mesophilic Bacteria ◽  
Functional Additives ◽  
High Level ◽  
Consumer Assessment

The aim of the study was to compare the microbiological safety and sensory quality of meat products manufactured with commercial functional additives. Four functional additives (AFX, AE100, PANA4, FPRX) were used in industrial conditions in the production of homogenized meat products (thick wiener). In order to determine the microbiological safety of final products, the total number of aerobic mesophilic bacteria and the number of Listeria monocytogenes were measured. Consumer assessment and quantitative flavor profiling (QFP) were used for analysis of organoleptic quality. After 7 days of storage, it was found that the effectiveness of the selected additives against the growth of aerobic mesophilic bacteria was unsatisfactory. Only after application of PANA4 did the product not show undesirable changes that would disqualify it from consumption. Each of the functional additives used had a high level of efficacy in inhibiting the growth of Listeria monocytogenes. Meat products with PANA4 addition had the highest consumer acceptance of the overall appearance. The best intensity of flavor bouquet, meat aroma and color, assessed by QFP method, was characterized by the samples with AFX and PANA4 in their recipe.

scholarly journals Effect of chitosan coating on the shelf life of ready-to-eat bovine meatballs and the control of Listeria monocytogenes growth on their surface during refrigeration storage

2019 ◽  
Vol 70 (2) ◽  
pp. 1495
Author(s):  
D. ANTONIADOU ◽  
A. GOVARIS ◽  
I. AMBROSIADIS ◽  
D. SERGELIDIS
Keyword(s):  
Listeria Monocytogenes ◽  
Shelf Life ◽  
Meat Products ◽  
Inhibitory Effect ◽  
Viable Count ◽  
Sensory Characteristics ◽  
Microbiological Analysis ◽  
Chitosan Coating ◽  
Chitosan Membranes ◽  
Refrigeration Storage

Edible chitosan coating on the surface of ready-to-eat (RTE) bovine meatballs was evaluated for its effect on their shelf life and the control of Listeria monocytogenes at 5 °C. L. monocytogenes was inoculated onto the surface of RTE bovine meatballs with and without edible chitosan coating. The samples were stored at 5 °C. Total aerobic viable count (TVC) and the bacterial counts of L. monocytogenes, lactic acid bacteria and Enterobacteriaceae were determined on days 0,1,7,14,21 and 28. The sensory characteristics were also evaluated at the same time spots by semi trained panelists. The results of the microbiological analysis depicted that the use of edible chitosan membranes reduced all of the microbial populations that were enumerated, and retarded their growth leading to the conclusion that they can prolong the shelf life of these products by 14 days. Moreover, the population of the inoculated L. monocytogenes was about 2 log CFU/g lower in the meatballs coated with chitosan, indicating an inhibitory effect of chitosan in the growth of L. monocytogenes. The sensory analysis showed that the samples coated with chitosan were satisfactorily accepted by the panelists even at day 28, in contrast to the samples without chitosan (control samples) which were unacceptable at day 14. These results indicate that edible chitosan coatings represent a potential agent in controlling L. monocytogenes on the surface of RTE meatballs as well as other RTE meat products, prolonging their shelf life without affecting their sensory characteristics.

Fate of Listeria monocytogenes in Commercial Ham, Formulated with or without Antimicrobials, under Conditions Simulating Contamination in the Processing or Retail Environment and during Home Storage

2007 ◽  
Vol 70 (2) ◽  
pp. 378-385 ◽  
Author(s):  
ALEXANDRA LIANOU ◽  
IFIGENIA GEORNARAS ◽  
PATRICIA A. KENDALL ◽  
KEITH E. BELK ◽  
JOHN A. SCANGA ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Shelf Life ◽  
Growth Rates ◽  
Meat Products ◽  
Storage Conditions ◽  
Risk Assessments ◽  
Worst Case ◽  
Potassium Lactate ◽  
Original Product ◽  
Pathogen Populations

Commercial cured ham formulated with or without potassium lactate and sodium diacetate was inoculated with Listeria monocytogenes and stored to simulate conditions of processing, retail, and home storage. The ham was sliced, inoculated with a 10-strain composite of L. monocytogenes (1 to 2 log CFU/cm2), vacuum packaged, and stored at 4°C to simulate contamination following lethality treatment at processing (first shelf life). After 10, 20, 35, and 60 days of storage, packages were opened, samples were tested, and bags with remaining slices were reclosed with rubber bands. At the same times, portions of original product (stored at 4°C in original processing bags) were sliced, inoculated, and packaged in delicatessen bags to simulate contamination during slicing at retail (second shelf life). Aerobic storage of both sets of packages at 7°C for 12 days was used to reflect domestic storage conditions (home storage). L. monocytogenes populations were lower (P < 0.05) during storage in ham formulated with lactate-diacetate than in product without antimicrobials under both contamination scenarios. Inoculation of ham without lactate-diacetate allowed prolific growth of L. monocytogenes in vacuum packages during the first shelf life and was the worst case contamination scenario with respect to pathogen numbers encountered during home storage. Under the second shelf life contamination scenario, mean growth rates of the organism during home storage ranged from 0.32 to 0.45 and from 0.18 to 0.25 log CFU/cm2/day for ham without and with lactate-diacetate, respectively, and significant increases in pathogen numbers (P < 0.05) were generally observed after 4 and 8 days of storage, respectively. Regardless of contamination scenario, 12-day home storage of product without lactate-diacetate resulted in similar pathogen populations (6.0 to 6.9 log CFU/cm2)(P ≥ 0.05). In ham containing lactate-diacetate, similar counts were found during the home storage experiment under both contamination scenarios, and only in 60-day-old product did samples from the first shelf life have higher (P < 0.05) pathogen numbers than those found in samples from the second shelf life. These results should be useful in risk assessments and for the establishment of “sell by” and “consume by” date labels for refrigerated ready-to-eat meat products.

Efficacy of pulsed light for shelf-life extension and inactivation of Listeria monocytogenes on ready-to-eat cooked meat products

2011 ◽  
Vol 12 (3) ◽  
pp. 275-281 ◽  
Author(s):  
Eva Hierro ◽  
Elvira Barroso ◽  
Lorenzo de la Hoz ◽  
Juan A. Ordóñez ◽  
Susana Manzano ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Shelf Life ◽  
Meat Products ◽  
Life Extension ◽  
Pulsed Light ◽  
Shelf Life Extension ◽  
Cooked Meat ◽  
Cooked Meat Products

scholarly journals Growth potential of Listeria monocytogenes in veal tartare

2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Simone Stella ◽  
Cristian Bernardi ◽  
Erica Tirloni
Keyword(s):  
Lactic Acid ◽  
Listeria Monocytogenes ◽  
Lactic Acid Bacteria ◽  
Shelf Life ◽  
Challenge Test ◽  
Growth Potential ◽  
Daily Maximum ◽  
Initial Concentration ◽  
Spoilage Bacteria ◽  
Sampling Times

In the present study the growth potential of Listeria monocytogenes in veal tartare was evaluated. A challenge test was performed on three tartare batches at 8°C, aiming to evaluate the growth potential of the pathogen. The data indicated the absence of a significant growth (δ<0.5 log cfu/g) during the entire period. When considering intermediate sampling times, an increase of 0.56 log cfu/g was detected after five days of storage in one of the batches. Microflora of veal tartare was dominated by lactic acid bacteria, that increased gradually during the trial, reaching counts up to 7 Log CFU/g in two of the three batches considered. Spoilage bacteria were present (especially Pseudomonas spp., yeasts and Enterobacteriaceae) but in very low counts and with a limited increase during the period considered. Finally, daily maximum tolerable L. monocytogenes counts were calculated to highlight the maximum acceptable load to avoid the overcoming of the legal limit of 100 CFU/g: a total increase of 0.95 log cfu/g in 12 days of shelf-life was estimated, obtaining a “safety initial concentration” at t0 of 10 CFU/g of the pathogen.

Effect of Inhibitory Extracts Derived from Liquid Smoke Combined with Postprocess Pasteurization for Control of Listeria monocytogenes on Ready-to-Eat Meats†

2007 ◽  
Vol 70 (12) ◽  
pp. 2749-2756 ◽  
Author(s):  
SARITHA GEDELA ◽  
RACHEL K. GAMBLE ◽  
SUNITA MACWANA ◽  
JOSEPH R. ESCOUBAS ◽  
PETER M. MURIANA
Keyword(s):  
Listeria Monocytogenes ◽  
Shelf Life ◽  
Meat Products ◽  
Radiant Heat ◽  
Department Of Agriculture ◽  
Log Reduction ◽  
Liquid Smoke ◽  
Heat Pasteurization ◽  
Inspection Service ◽  
The U.S

Surface pasteurization was examined in combination with low-phenolic antimicrobial extracts derived from liquid smoke to inhibit and prevent the growth of Listeria monocytogenes during the shelf life of ready-to-eat meats. In preliminary trials with retail frankfurters, one smoke derivative (2-min dip) produced a 0.3-log reduction of L. monocytogenes and a 1-min inbag pasteurization (73.9°C) produced a 2.9-log reduction, whereas a combination of the two treatments produced a 5.3-log reduction that resulted in no detectable Listeria by week 3 under accelerated shelf-life conditions (10°C). In trials with frankfurters manufactured without lactate or diacetate that were treated with a shortened 1-s dip, this smoke extract and one with reduced smoke flavor and color both produced a &gt;4.5-log reduction of L. monocytogenes on frankfurters when heated at 73.9°C for 1 min, with no recoverable Listeria detected for 10 weeks when stored at 6.1°C. When deli turkey breast chubs manufactured without lactate, diacetate, or nitrite were treated with a 1-s dip in combination with radiant-heat pasteurization (270°C), growth of L. monocytogenes was retarded but not prevented. However, in a similar study in which smoke extract treatment of deli turkey breast was combined with in-bag postpackage pasteurization (water submersion at 93.3°C), a 60-, 45-, or even 30-s heat treatment resulted in a 2- to 3-log reduction of L. monocytogenes, with no growth on the meat during 10 weeks of storage at 6.1°C. These findings indicate that reduced-acid low-phenolic antimicrobial liquid smoke derivatives combined with surface pasteurization are capable of reducing or preventing growth of L. monocytogenes to meet the criteria for the U.S. Department of Agriculture Food Safety and Inspection Service Alternative 1 process for ready-to-eat deli meat products manufactured without lactate or diacetate.

Prevalence and Genetic Diversity of Listeria monocytogenes in Vacuum-Packaged Ready-to-Eat Meat Products at Retail Markets in Latvia

2009 ◽  
Vol 72 (6) ◽  
pp. 1283-1287 ◽  
Author(s):  
AIVARS BĒRZIŅŠ ◽  
MARGARITA TERENTJEVA ◽  
HANNU KORKEALA
Keyword(s):  
Listeria Monocytogenes ◽  
Shelf Life ◽  
Meat Products ◽  
Processing Plant ◽  
Meat Processing ◽  
Pork Products ◽  
Beef Products ◽  
Processing Plants ◽  
Serotype 1 ◽  
Packaged Beef

Nine groups of different retail ready-to-eat vacuum-packaged meat products from 10 Baltic meat processing plants were analyzed for presence and numbers of Listeria monocytogenes at the end of shelf life. A total of 38 (18%) of 211 samples tested positive for L. monocytogenes serotype 1/2a (88%) or 1/2c (12%). The prevalence of L. monocytogenes in cold-smoked, sliced, vacuum-packaged beef and pork products (42%) was significantly higher than in cooked, sliced, vacuum-packaged meat products (0.8%) (P &lt; 0.001). Enumeration of L. monocytogenes showed that 84% of the positive samples contained &lt;100 CFU/g upon expiry of product shelf life. The numbers of L. monocytogenes exceeded 100 CFU/g only in cold-smoked, sliced, vacuum-packaged beef products. Identical pulsed-field gel electrophoresis types were recovered from different production lots of cold-smoked vacuum-packaged beef and pork products produced by the same meat processing plant, demonstrating L. monocytogenes contamination as a recurrent problem within one meat processing plant.

scholarly journals Antimicrobial and Antioxidative Effects of Plant Powders in Raw and Cooked Minced Pork

Foods ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 661 ◽  
Author(s):  
Dea Anton ◽  
Julia Koskar ◽  
Piret Raudsepp ◽  
Kadrin Meremäe ◽  
Tanel Kaart ◽  
...  
Keyword(s):  
Lipid Oxidation ◽  
Shelf Life ◽  
Microbial Growth ◽  
Challenge Test ◽  
Meat Products ◽  
Thiobarbituric Acid ◽  
Growth Potential ◽  
Plant Materials ◽  
Plant Powders ◽  
The Impact

It is a challenge for scientists to find new plant-based food constituents simultaneously possessing antimicrobial and antioxidative properties to prolong the shelf life of meat products. In this study, various plant powders and their blends were added to minced pork to carry out a complex study of their effect on sensory characteristics, microbial growth, and lipid oxidation of the meat in raw and cooked forms during storage. Microbiological shelf life parameters were evaluated by determining the total counts of microorganisms, yeasts, and molds. The growth potential of Listeria monocytogenes was estimated by challenge testing. The impact on lipid oxidation processes was assessed using thiobarbituric acid reactive substances (TBARS) and high performance liquid chromatography (HPLC) methods. The results showed that the blend of rhubarb petioles and tomato powder added a pleasant color and a combined taste to the product, similar to the taste of salt. In raw samples, considerable microbial growth inhibition was achieved with rhubarb petioles, tomato, and their mixture. Nine treatments of cooked samples had a stronger inhibitory effect on microbial growth compared to control treatments. Among all plant powders, tomato was the most effective inhibitor of yeast and mold growth. However, the challenge test revealed that L. monocytogenes growth in cooked samples was not inhibited during shelf life. In raw samples, rhubarb roots combined with blackcurrant or chokeberry berries effectively inhibited lipid oxidation, and in cooked samples, rhubarb petioles showed a similar effect. In conclusion, it was found that powdered plant materials are well suited for use as antimicrobial and antioxidative agents in minced meat products.

scholarly journals Prevalence of Listeria monocytogenes in pork-meat and other processed products from the Colombian swine industry

2012 ◽  
pp. 2827-2833 ◽  
Author(s):  
Andrea Gamboa-Marín ◽  
Sonia Buitrago M ◽  
Karol Pérez-Pérez ◽  
Marcela Mercado R ◽  
Raúl Poutou-Piñales ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Listeria Monocytogenes ◽  
Multiplex Pcr ◽  
Meat Products ◽  
Stratified Sampling ◽  
Pork Meat ◽  
Swine Industry ◽  
Processed Products

ABSTRACTObjective. To determine the prevalence of L. monocytogenes in pork carcasses, meat cuts, and meat products (“chorizo”, sausage and ham). Materials and methods. Stratified sampling was implemented in meat-processed products. We analyzed 566 (37%) carcasses, 472 (31%) meat cuts, and 481, (32%) meat-processed products, distributed as follows: 169 (11%) sausage, 163 (11%) ham, and 149 (10%) “chorizo”, for a total of 1519 (100%) samples in a period of 18 months. The samples were processed using the ISO-17604, ISO-11290-1 and the USDA/FSIS (MLG-8.03) methods. Genus and species were confirmed by multiplex-PCR. Results. We obtained isolates of L. monocytogenes from 21 carcasses (10%), 160 (76%) from meat deboning, 10 (5%) from ham, 6 (3%) from “chorizo”, and 13 (6%) from sausage. The prevalence found was 3.7% and 33.9% in carcasses and meat deboning respectively. The prevalence in the meat-processed products was 4.03% in “chorizo”, 6.13% in ham and 7.69% in sausage. The overall prevalence of L. monocytogenes in the study was 13.82%. Conclusions. We found L. monocytogenes in different products analyzed, with particular interest in ham and sausage since both are consumed without previous heat treatment

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