scholarly journals High Hydrostatic Pressure Assisted by Celluclast® Releases Oligosaccharides from Apple By-Product

Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1058
Author(s):  
Rocío De la Peña-Armada ◽  
María José Villanueva-Suárez ◽  
Pilar Rupérez ◽  
Inmaculada Mateos-Aparicio
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Dietary Fibre ◽  
Hplc Method ◽  
Water Soluble ◽  
Food Ingredient ◽  
Promising Alternative ◽  
Cellulase Treatment ◽  
Time Required ◽  
Green Procedure

A novel and green procedure consisting of high hydrostatic pressure (HHP) aided by a commercial cellulase (Celluclast®) has been applied to valorise the apple by-product, a valuable source of dietary fibre but mainly composed by insoluble fibre. Optimal conditions for solubilisation of dietary fibre were first determined at atmospheric pressure as 2% (w/v) of substrate concentration and 20 Endo-Glucanase Units of cellulase. Monitoring of polysaccharides and oligosaccharides released from apple by-product was carried out by means of a newly validated HPLC method with refractive index detector. A synergistic effect was observed when the combined HHP plus cellulase treatment was used. Thus, the application of 200 MPa at 50 °C for 15 min enabled a significant increase in the release of water-soluble polysaccharides (1.8-fold) and oligosaccharides (3.8-fold), as well as a considerable decrease in the time required (up to 120-fold), compared to control at 0.1 MPa. Therefore, this technology could be a promising alternative approach to transform an industrial by-product into a novel rich-in-oligosaccharide food ingredient and a step forward into shaping the world of prebiotics.

scholarly journals A Novel Proteomic Analysis of the Modifications Induced by High Hydrostatic Pressure on Hazelnut Water-Soluble Proteins

Foods ◽  
2014 ◽  
Vol 3 (2) ◽  
pp. 279-289 ◽  
Author(s):  
Nuria Prieto ◽  
Carmen Burbano ◽  
Elisa Iniesto ◽  
Julia Rodríguez ◽  
Beatriz Cabanillas ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Proteomic Analysis ◽  
Soluble Proteins ◽  
Water Soluble

Valorisation Approach for the Soybean By-Product Okara Using High Hydrostatic Pressure

2019 ◽  
Vol 15 (6) ◽  
pp. 548-550 ◽  
Author(s):  
Inmaculada Mateos-Aparicio ◽  
Elena Pérez-López ◽  
Pilar Rupérez
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Dietary Fibre ◽  
New Technologies ◽  
Scale Up ◽  
Industrial Transformation ◽  
Novel Approach ◽  
Soluble Dietary Fibre ◽  
Carcinogenic Effects ◽  
The Rich

Okara is a perishable, cheap and abundant by-product derived from soybean after extracting the soluble fraction for tofu or soybean drink, mainly known as soymilk, production. Nowadays, Okara is mostly discarded: landfill and incineration, but a useful alternative for valorisation would be to use it as a valuable source of dietary fibre. However, it presents low soluble dietary fibre (SDF) content responsible for prebiotic and anti-carcinogenic effects, so an easy industrial transformation to maximize its SDF content would be most interesting for this purpose. Different approaches can be used to increase SDF content, such as chemical or enzymatic treatments with food-grade enzymes at atmospheric pressure, but these conventional methods present some disadvantages as that the chemical procedures are pollutant and the extractions normally are incomplete, and the enzymatic methods could be expensive to scale-up. On the other hand, currently, consumers are demanding for safer, more natural and minimally-processed foods. This request has led researchers and manufacturers to develop new technologies, and within these, high hydrostatic pressure (HHP) is one of the top-10 most popular emerging technologies applied in the field of food science. The effect of HHP, and more recently, the combined effect of HHP and enzymatic treatment on okara by-product have been studied, showing that this novel approach, should also be considered in order to stabilise other agro-food byproducts -due to their perishable character- as well as to improve the functionality of the rich-ininsoluble dietary fibre from vegetable residues.

Antimicrobial effect of water‐soluble chitosans with high hydrostatic pressure

1991 ◽  
Vol 5 (1) ◽  
pp. 45-57 ◽  
Author(s):  
Anne M. Papineau ◽  
Dallas G. Hoover ◽  
Dietrich Knorr ◽  
Daniel F. Farkas
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Antimicrobial Effect ◽  
Water Soluble ◽  
Effect Of Water

Application of preliminary high-pressure processing for improving bioactive characteristics and reducing antigenicity of whey protein hydrolysates

2021 ◽  
pp. 108201322110221
Author(s):  
Ana PM Landim ◽  
Natália K Matsubara ◽  
José E da Silva-Santos ◽  
Caroline Mellinger-Silva ◽  
Amauri Rosenthal
Keyword(s):  
Hydrostatic Pressure ◽  
Antioxidant Capacity ◽  
Whey Protein ◽  
High Hydrostatic Pressure ◽  
Bioactive Peptides ◽  
Soluble Proteins ◽  
High Pressure Processing ◽  
Promising Alternative ◽  
Pre Treatment

This study investigated the use of Novo Pro-D® (NPD) and Ficin (FC) as alternative proteases for the production of bioactive peptides with reduced allergenicity from whey protein concentrate (WPC). In addition, the use of high hydrostatic pressure processing as pre-treatment of WPC and its impact on the final characteristics of hydrolysates were also evaluated. NPD treatments generated hydrolysates with a 98% reduction of soluble proteins, greater in vitro antioxidant capacity, and less immunoreactivity when compared to FC ones. However, pre-treatment was an essential tool to improve WPC hydrolysis when FC was used, resulting in hydrolysates with less soluble proteins, enhanced antioxidant capacity, and less allergenicity compared with conventional hydrolysis. As for NPD, the pre-treatment of WPC improved the in vitro antioxidant capacity and resulted in a 100% reduction in immunoreactivity to β-lactoglobulin in a shorter processing time. Importantly, bioactive peptides generated by FC displayed an improved ability to induce in vitro arterial relaxation, compared with those obtained from NPD process. Therefore, this study provides innovative evidence regarding how the proteases used for production of whey hydrolysates can improve its biological effects, and discloses the use of high hydrostatic pressure combined with enzymatic hydrolysis as a promising alternative to produce hydrolysates with improved properties.

scholarly journals Devitalizing Effect of High Hydrostatic Pressure on Human Cells—Influence on Cell Death in Osteoblasts and Chondrocytes

2020 ◽  
Vol 21 (11) ◽  
pp. 3836
Author(s):  
Janine Waletzko ◽  
Michael Dau ◽  
Anika Seyfarth ◽  
Armin Springer ◽  
Marcus Frank ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Death ◽  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Cell Damage ◽  
Water Soluble ◽  
Cartilage Tissue ◽  
Initial Assessment ◽  
Tetrazolium Salt ◽  
Tissue Samples

Chemical and physical processing of allografts is associated with a significant reduction in biomechanics. Therefore, treatment of tissue with high hydrostatic pressure (HHP) offers the possibility to devitalize tissue gently without changing biomechanical properties. To obtain an initial assessment of the effectiveness of HHP treatment, human osteoblasts and chondrocytes were treated with different HHPs (100–150 MPa, 250–300 MPa, 450–500 MPa). Devitalization efficiency was determined by analyzing the metabolic activity via WST-1(water-soluble tetrazolium salt) assay. The type of cell death was detected with an apoptosis/necrosis ELISA (enzyme-linked immune sorbent assay) and flow cytometry. Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) were carried out to detect the degree of cell destruction. After HHP treatment, the metabolic activities of both cell types decreased, whereas HHP of 250 MPa and higher resulted in metabolic inactivation. Further, the highest HHP range induced mostly necrosis while the lower HHP ranges induced apoptosis and necrosis equally. FESEM and TEM analyses of treated osteoblasts revealed pressure-dependent cell damage. In the present study, it could be proven that a pressure range of 250–300 MPa can be used for cell devitalization. However, in order to treat bone and cartilage tissue gently with HHP, the results of our cell experiments must be verified for tissue samples in future studies.

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