scholarly journals Development and Validation of a Gene-Targeted dCAPS Marker for Marker-Assisted Selection of Low-Alkaloid Content in Seeds of Narrow-Leafed Lupin (Lupinus angustifolius L.)

Genes ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 428 ◽  
Author(s):  
Magdalena Kroc ◽  
Katarzyna Czepiel ◽  
Paulina Wilczura ◽  
Monika Mokrzycka ◽  
Wojciech Święcicki
Keyword(s):  
Marker Assisted Selection ◽  
Dry Weight ◽  
Promoter Sequence ◽  
Lupinus Angustifolius ◽  
Alkaloid Content ◽  
Accelerate Development ◽  
Dcaps Marker ◽  
Marker Band ◽  
Lupin Seeds ◽  
Development And Validation

Low-alkaloid content is an important breeding target to improve the quality of lupin seeds. An APETALA2/ethylene response transcription factor, RAP2-7, is likely a candidate gene for the major alkaloid locus iucundus, and plays a crucial role in regulation of seed alkaloid content in narrow-leafed lupin (NLL; Lupinus angustifolius L.). Here, we exploited a single-nucleotide polymorphism within RAP2-7 credibly associated with seed alkaloid content, to develop the co-dominant derived cleaved amplified polymorphic sequence (dCAPS) marker iuc_RAP2-7. Marker validation in 202 NLL accessions demonstrated that seed alkaloid content ≥0.9% of the seed dry weight was associated with the high-alkaloid marker band (Iucundus genotypes), whereas alkaloid content up to 0.5% of the seed dry weight was associated with the low-alkaloid marker band (iucundus genotypes). Within a given detection limit, iuc_RAP2-7 unambiguously identified all but three low-alkaloid accessions. The latter accessions apparently have a different regulatory mechanism for seed alkaloid content because the RAP2-7 gene/putative promoter sequence and expression of alkaloid-associated genes in the leaves of the three ambiguous accessions were similar to those of bitter Iucundus lines. We consider the iuc_RAP2-7 marker is a powerful tool that will facilitate NLL marker-assisted selection by rapid rejection of bitter Iucundus genotypes and thus accelerate development of new low-alkaloid cultivars.

scholarly journals COLORIMETRIC VS. CHROMATOGRAPHIC ANALYSES OF ALKALOIDS IN LUPIN SEEDS

2018 ◽  
Vol 78 ◽  
pp. 63-67
Author(s):  
Paulina Wilczura ◽  
Wojciech Święcicki ◽  
Katarzyna Kamel ◽  
Wiesław Wasiak
Keyword(s):  
Bitter Taste ◽  
Lupinus Albus ◽  
Colorimetric Method ◽  
Dry Weight ◽  
Alkaloid Content ◽  
Estimation Methods ◽  
Material Development ◽  
Colorimetric Test ◽  
Lupin Seeds ◽  
Accurate Quantification

A characteristic trait of lupins is a production of alkaloids, which are a toxic and bitter taste compound ofseeds. Due to the lack of fast, sensitive and inexpensive screening techniques to identify and reject high alkaloidplant material, development of suitable tools is important challenges for lupins breeding and seed production.The aim of this study was to compare two alkaloid content estimation methods in Lupinus angustifoliusL. and Lupinus albus L.During the Wagner’s colorimetric test, which is recommended by the UPOV, seed halves were stained onfour colors depending on the alkaloid content but only the level of 0.5% – 0.6% showed clear color change.Gas chromatography allowed accurate quantification and qualification of alkaloid content.Since safe alkaloid content for consumption is 0.02% of seed dry weight, colorimetric method is less usefulfor dividing lupin cultivars into sweet and bitter, than gas chromatography but can be used as a screeningtechnique.

Adaptation of Lupinus angustifolius L. and L. pilosus Murr. to calcareous soils

1999 ◽  
Vol 50 (6) ◽  
pp. 1027 ◽  
Author(s):  
J. D. Brand ◽  
C. Tang ◽  
A. J. Rathjen
Keyword(s):  
Calcareous Soils ◽  
South Australia ◽  
Clay Content ◽  
Dry Weight ◽  
Lupinus Angustifolius ◽  
Nutrient Concentrations ◽  
Carbonate Content ◽  
Soil Factors ◽  
Shoot Dry Weight ◽  
Caco3 Content

Current varieties of narrow-leafed lupin (Lupin angustifolius L.) are poorly adapted to alkaline and calcareous soils found commonly throughout the south-estern Australian cropping zone. Apot experiment compared the growth of Lupinus angustifolius cv. Gungurru with L. pilosus P20954 in a range of soils collected throughout South Australia. The soils displayed a range of texture (clay, 3–82%), pH (1:5 soil:H2O, 7·0–9·6), and calcium carbonate content (CaCO3, 0–47%). Potting mix (pH 5·8) was used as the control. The plants were grown for 7 weeks with weekly measurements of chlorosis score and leaf number. At harvest, dry weights were recorded and the youngest fully expanded leaves were analysed for nutrient concentrations. The line P20954 grew much better in all the soils than Gungurru in terms of plant dry weight relative to the control soil, this being particularly evident in the calcareous soils. Chlorosis score correlated highly with shoot dry weight for Gungurru, but not for P20954. The main soil factor contributing to the chlorosis score of Gungurru was CaCO3 content, whereas none of the soil factors significantly affected P20954, although in Weeks 2 and 3 chlorosis score correlated with CaCO3 content. The dry weight of Gungurru was affected by a combination of factors including clay content, pH, and CaCO3 content, whereas the dry weight of P20954 was affected by most of the soil factors measured. The dry weight of P20954 was positively correlated with aluminium and magnesium concentrations. Concentrations of all nutrients were above critical levels for both genotypes grown in all soils. The results indicate that L. pilosus has the potential to be grown in areas where current varieties of L. angustifolius are poorly adapted.

scholarly journals Functional Analysis Reveals the Regulatory Role of PpTST1 Encoding Tonoplast Sugar Transporter in Sugar Accumulation of Peach Fruit

2020 ◽  
Vol 21 (3) ◽  
pp. 1112 ◽  
Author(s):  
Peng ◽  
Wang ◽  
Ogutu ◽  
Liu ◽  
Liu ◽  
...  
Keyword(s):  
Sugar Content ◽  
Subcellular Location ◽  
Promoter Sequence ◽  
Sugar Accumulation ◽  
Sugar Transporter ◽  
Peach Fruit ◽  
Accumulation Pattern ◽  
Dcaps Marker ◽  
Significant Difference

Sugar content is related to fruit sweetness, and the complex mechanisms underlying fruit sugar accumulation still remain elusive. Here, we report a peach PpTST1 gene encoding tonoplast sugar transporter that is located in the quantitative trait loci (QTL) interval on Chr5 controlling fruit sucrose content. One derived Cleaved Amplified Polymorphic Sequence (dCAPS) marker was developed based on a nonsynonymous G/T variant in the third exon of PpTST1. Genotyping of peach cultivars with the dCAPS marker revealed a significant difference in fruit sugar content among genotypes. PpTST1 is located in the tonoplast, and substitution of glutamine by histidine caused by the G/T variation has no impact on subcellular location. The expression profile of PpTST1 exhibited a consistency with the sugar accumulation pattern, and its transient silencing significantly inhibited sugar accumulation in peach fruits. All of these results demonstrated the role of PpTST1 in regulating sugar accumulation in peach fruit. In addition, cis-elements for binding of MYB and WRKY transcript factors were found in the promoter sequence of PpTST1, suggesting a gene regulatory network of fruit sugar accumulation. Our results are not only helpful for understanding the mechanisms underlying fruit sugar accumulation, but will also be useful for the genetic improvement of fruit sweetness in peach breeding programs.

Fruit development and associated changes in the distribution of dry weight and nitrogen in Lupinus angustifolius cv. Uniharvest and L. consentinii selection CB12

1976 ◽  
Vol 16 (80) ◽  
pp. 387 ◽  
Author(s):  
P Farrington
Keyword(s):  
Nitrogen Content ◽  
Fruit Development ◽  
Seed Weight ◽  
Dry Matter ◽  
Reproductive Development ◽  
Dry Weight ◽  
Lupinus Angustifolius ◽  
Rapid Fall

Reproductive development, and the distribution of dry matter and nitrogen were followed in field plantings of Lupinus angustifolius cv. Uniharvest and L. cosentinii selection CB12 from the start of flowering until maturity. L. cosentinii (CBI 2) commenced flowering one week earlier, but developed one less order of inflorescences and fewer flowers than L. angustfiolius (Uniharvest) ; yet it set more pods and produced more seed. In both species seeds did not commence to fill until the leaves began to fall shortly after flowering ended. Seeds in pods on all orders of inflorescence filled concurrently. The increase in seed weight coincided with a rapid fall in the nitrogen content of other fractions of the tops.

scholarly journals Development and Validation of High-Resolution Melting Markers Derived from Rysto STS Markers for High-Throughput Marker-Assisted Selection of Potato Carrying Rysto

2016 ◽  
Vol 106 (11) ◽  
pp. 1366-1375 ◽  
Author(s):  
Xianzhou Nie ◽  
Darcy Sutherland ◽  
Virginia Dickison ◽  
Mathuresh Singh ◽  
Agnes M. Murphy ◽  
...  
Keyword(s):  
High Resolution ◽  
High Throughput ◽  
Marker Assisted Selection ◽  
High Resolution Melting ◽  
Chromosome Region ◽  
Sts Markers ◽  
Reverse Primer ◽  
Selection For ◽  
Development And Validation ◽  
Selection Of

Sequence analysis of the chromosome region harboring the sequence-tagged site (STS) markers YES3-3A and YES3-3B for Rysto, a gene responsible for extreme resistance to Potato virus Y (PVY) in potato, was performed in tetraploid potato ‘Barbara’ (Rrrr) and ‘AC Chaleur’ (rrrr) as well as their progeny selections. Three and two sequence variants were identified in Barbara resistant (R) selections and AC Chaleur susceptible (S) selections, respectively. Further analysis indicates that the variant with a 21-nucleotide (nt) deletion is likely the chromosome copy harboring the STS markers. Two primer pairs, one targeting the region containing a 20-nt deletion and the other targeting the region anchoring the YES3-3A reverse primer, were designed. As anticipated, pair one produced two visible fragments in Barbara-R bulk and one visible fragment in AC Chaleur-S bulk; pair two produced one visible fragment in all samples. When subjected to high-resolution melting (HRM) analysis, two distinct melting profiles for R and S samples were observed. Analysis of 147 progeny of Barbara × AC Chaleur revealed 72 and 75 progeny with R and S melting profiles, respectively, which was consistent with YES3-3A and YES3-3B assays and phenotyping analysis, thus demonstrating the potential of HRM profiles as novel molecular markers for Rysto. The efficacy of the newly developed HRM markers for high-throughput marker-assisted selection for Rysto-conferred resistance to PVY was validated further with three populations involving Barbara as the R parent.

scholarly journals Lamalbid, Chlorogenic Acid, and Verbascoside as Tools for Standardization of Lamium album Flowers—Development and Validation of HPLC–DAD Method

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1721
Author(s):  
Monika E. Czerwińska ◽  
Eleonora Kalinowska ◽  
Dominik Popowski ◽  
Agnieszka Bazylko
Keyword(s):  
Chlorogenic Acid ◽  
High Performance ◽  
Dry Weight ◽  
Upper Respiratory Tract ◽  
Topical Medication ◽  
Traditional Medicines ◽  
Array Detection ◽  
Upper Respiratory ◽  
Development And Validation ◽  
Lamium Album

Preparations from the flowers or herb of the white dead nettle (Lamium album L.) are recommended for the treatment of upper respiratory tract disorders or as a topical medication for mild inflammation of the throat, mouth, and skin. Taking into consideration the significance of L. album in traditional medicines across Europe, as well as the lack of studies describing the quantities of their most abundant constituents, we aimed to design a high-performance liquid chromatography coupled with diode-array detection (HPLC–DAD) method for potential standardization procedures of extracts from flowers of L. album. The HPLC–DAD method was developed and validated for quantification of iridoids (lamalbid), phenolic acids/depsides (chlorogenic acid), phenylpropanoids (verbascoside), and flavonoids (rutin; quercetin malonylhexoside; tiliroside) in aqueous and ethanolic-aqueous extracts of Lamii albi flos. The method was specific, accurate, and precise. Lamalbid was the most abundant compound both in aqueous (39.09 ± 1.02 m/g dry weight) and ethanolic-aqueous (26.66 ± 0.64 m/g dry weight) extracts. The quantities of selected compounds, except for chlorogenic acid and tiliroside, were higher in the aqueous extract than in the ethanolic-aqueous one. In conclusion, the method developed allowed for quantitation of compounds from different classes. In particular, chlorogenic acid and verbascoside have been proposed as reference compounds for routine quantitative control of Lamii albi flos.

Equilibrium between basic nitrogen compounds in lupin seeds with differentiated alkaloid content

2001 ◽  
Vol 57 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Tadeusz Aniszewski ◽  
Danuta Ciesiołka ◽  
Krzysztof Gulewicz
Keyword(s):  
Alkaloid Content ◽  
Nitrogen Compounds ◽  
Basic Nitrogen ◽  
Lupin Seeds

Agronomical and chemical variability of Colchicum autumnale accessions

2006 ◽  
Vol 86 (2) ◽  
pp. 547-555 ◽  
Author(s):  
A. Poutaraud and P. Girardin
Keyword(s):  
Genetic Variability ◽  
Medicinal Plant ◽  
Key Words ◽  
Vegetative Propagation ◽  
Dry Weight ◽  
Alkaloid Content ◽  
Colchicum Autumnale ◽  
Chemical Variability ◽  
Vegetative Multiplication ◽  
Key Words Colchicine

Meadow saffron (Colchicum autumnale) is an undomesticated plant from whose seeds the alkaloids colchicine and colchicoside are extracted and used pharmaceutically. Research was conducted towards domesticating meadow saffron in which inter- and intra-genetic variability of accessions was studied. Colchicine, 3-demethylcolchicine and colchicoside were assayed in plants of six accessions collected from natural sites in eastern France. These were cultivated at two sites and studied during 1 or 2 yr. Plants from 70 initial transplanted corms of one accession were studied individually for 4 yr to determined intra-accession variability and, as spontaneous vegetative multiplication of corms occurred, comparisons among plants from one initial transplanted corm were also made. For the main characteristics, seed dry weight (SDW) per plant, alkaloid content and colchicine:colchicoside ratio, the inter-accession variability was lower than the intra-accession variability. The results suggest that vegetative propagation of selected genotypes could improve alkaloid content by about 80% and improve SDW per plant by about 300%. Key words: Colchicine, colchicoside, Colchicum autumnale, medicinal plant, genetic variability

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